JPS5621599A - Kit for determination of phosphoric acid using pyruvate oxidase, and method for determination using the same - Google Patents
Kit for determination of phosphoric acid using pyruvate oxidase, and method for determination using the sameInfo
- Publication number
- JPS5621599A JPS5621599A JP9839179A JP9839179A JPS5621599A JP S5621599 A JPS5621599 A JP S5621599A JP 9839179 A JP9839179 A JP 9839179A JP 9839179 A JP9839179 A JP 9839179A JP S5621599 A JPS5621599 A JP S5621599A
- Authority
- JP
- Japan
- Prior art keywords
- phosphoric acid
- acid
- specimen
- determination
- kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 title abstract 14
- 229910000147 aluminium phosphate Inorganic materials 0.000 title abstract 7
- 108010042687 Pyruvate Oxidase Proteins 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 abstract 4
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 abstract 2
- VWWQXMAJTJZDQX-UHFFFAOYSA-N Flavine adenine dinucleotide Natural products C1=NC2=C(N)N=CN=C2N1C(C(O)C1O)OC1COP(O)(=O)OP(O)(=O)OCC(O)C(O)C(O)CN1C2=NC(=O)NC(=O)C2=NC2=C1C=C(C)C(C)=C2 VWWQXMAJTJZDQX-UHFFFAOYSA-N 0.000 abstract 2
- JLTDJTHDQAWBAV-UHFFFAOYSA-N N,N-dimethylaniline Chemical compound CN(C)C1=CC=CC=C1 JLTDJTHDQAWBAV-UHFFFAOYSA-N 0.000 abstract 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 abstract 2
- VWWQXMAJTJZDQX-UYBVJOGSSA-N flavin adenine dinucleotide Chemical compound C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1CO[P@](O)(=O)O[P@@](O)(=O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C2=NC(=O)NC(=O)C2=NC2=C1C=C(C)C(C)=C2 VWWQXMAJTJZDQX-UYBVJOGSSA-N 0.000 abstract 2
- QRMZSPFSDQBLIX-UHFFFAOYSA-N homovanillic acid Chemical compound COC1=CC(CC(O)=O)=CC=C1O QRMZSPFSDQBLIX-UHFFFAOYSA-N 0.000 abstract 2
- 229940107700 pyruvic acid Drugs 0.000 abstract 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 abstract 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 abstract 1
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 abstract 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 abstract 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 abstract 1
- 241000192001 Pediococcus Species 0.000 abstract 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 abstract 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 239000001110 calcium chloride Substances 0.000 abstract 1
- 229910001628 calcium chloride Inorganic materials 0.000 abstract 1
- 229910001424 calcium ion Inorganic materials 0.000 abstract 1
- 239000012295 chemical reaction liquid Substances 0.000 abstract 1
- 238000004737 colorimetric analysis Methods 0.000 abstract 1
- 235000014113 dietary fatty acids Nutrition 0.000 abstract 1
- 239000000194 fatty acid Substances 0.000 abstract 1
- 229930195729 fatty acid Natural products 0.000 abstract 1
- 150000004665 fatty acids Chemical class 0.000 abstract 1
- 238000005558 fluorometry Methods 0.000 abstract 1
- 239000011565 manganese chloride Substances 0.000 abstract 1
- 230000036284 oxygen consumption Effects 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 229960002363 thiamine pyrophosphate Drugs 0.000 abstract 1
- 235000008170 thiamine pyrophosphate Nutrition 0.000 abstract 1
- 239000011678 thiamine pyrophosphate Substances 0.000 abstract 1
- YXVCLPJQTZXJLH-UHFFFAOYSA-N thiamine(1+) diphosphate chloride Chemical compound [Cl-].CC1=C(CCOP(O)(=O)OP(O)(O)=O)SC=[N+]1CC1=CN=C(C)N=C1N YXVCLPJQTZXJLH-UHFFFAOYSA-N 0.000 abstract 1
- 210000002700 urine Anatomy 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9839179A JPS5621599A (en) | 1979-07-31 | 1979-07-31 | Kit for determination of phosphoric acid using pyruvate oxidase, and method for determination using the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9839179A JPS5621599A (en) | 1979-07-31 | 1979-07-31 | Kit for determination of phosphoric acid using pyruvate oxidase, and method for determination using the same |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5621599A true JPS5621599A (en) | 1981-02-28 |
JPS6140400B2 JPS6140400B2 (enrdf_load_stackoverflow) | 1986-09-09 |
Family
ID=14218537
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP9839179A Granted JPS5621599A (en) | 1979-07-31 | 1979-07-31 | Kit for determination of phosphoric acid using pyruvate oxidase, and method for determination using the same |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5621599A (enrdf_load_stackoverflow) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6265179B1 (en) | 2000-02-01 | 2001-07-24 | Molecular Probes, Inc. | Detection of phosphate using coupled enzymatic reactions |
-
1979
- 1979-07-31 JP JP9839179A patent/JPS5621599A/ja active Granted
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6265179B1 (en) | 2000-02-01 | 2001-07-24 | Molecular Probes, Inc. | Detection of phosphate using coupled enzymatic reactions |
Also Published As
Publication number | Publication date |
---|---|
JPS6140400B2 (enrdf_load_stackoverflow) | 1986-09-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA1136027A (en) | Method for determining a substance in a biological fluid and reagent combination for use in the method | |
CA1148456A (en) | Process and reagent for determining triglycerides | |
Berti et al. | Enzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine | |
JPS5581586A (en) | Acidic uricase | |
CA1227113A (en) | Enzymatic atp/fmn assay | |
DE3065827D1 (en) | Method of stabilizing an enzyme solution for use in total cholesterol determination, stabilized solution and test kit therefor | |
EP0533183B1 (en) | Composition for the measurement of potassium ion concentration | |
EP0183381B1 (en) | Method for the determination of cholesterol | |
JPS55108297A (en) | Determining method of free fatty acid | |
JPS5621599A (en) | Kit for determination of phosphoric acid using pyruvate oxidase, and method for determination using the same | |
GB2028500A (en) | Method for determining a transminase in a biological fluid and reagent combinations for use in the methods | |
CA2045704A1 (en) | Stable aqueous nadh reagent and kit | |
Winartasaputra et al. | Fluorometric and colorimetric enzymic determination of triglycerides (triacylglycerols) in serum. | |
Graves et al. | Metabolism of Pyruvate by Epididymal-Like Bovine Spermatozoa | |
JPH042240B2 (enrdf_load_stackoverflow) | ||
Bieth et al. | A colorimetric method for measuring the esterolytic activity of elastase | |
DE3403250C2 (de) | Neue hochempfindliche enzymatische Testmethode | |
JPS56164797A (en) | Improved method of determining components in samples | |
CA1235635A (en) | Process and reagent for the determination of glycerol | |
AU2680788A (en) | Self-indicating and improved resolution analyses employing stoichiometric chemical substraction | |
JP2534859B2 (ja) | 生体物質のエンザイムアツセイ | |
EP0045032B1 (en) | A triglyceride analysis composition and a method for triglyceride determination | |
JPS55148100A (en) | Determination of aminotransferase using novel reagent kit for determination of hydrogen peroxide | |
JPS55104899A (en) | Reagent for determining triglyceride in serum | |
CA2000963A1 (en) | Method of assaying magnesium |