JPS5621599A - Kit for determination of phosphoric acid using pyruvate oxidase, and method for determination using the same - Google Patents

Kit for determination of phosphoric acid using pyruvate oxidase, and method for determination using the same

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Publication number
JPS5621599A
JPS5621599A JP9839179A JP9839179A JPS5621599A JP S5621599 A JPS5621599 A JP S5621599A JP 9839179 A JP9839179 A JP 9839179A JP 9839179 A JP9839179 A JP 9839179A JP S5621599 A JPS5621599 A JP S5621599A
Authority
JP
Japan
Prior art keywords
phosphoric acid
acid
specimen
determination
kit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP9839179A
Other languages
Japanese (ja)
Other versions
JPS6140400B2 (en
Inventor
Hideo Misaki
Kazuo Matsuura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyo Jozo KK
Original Assignee
Toyo Jozo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyo Jozo KK filed Critical Toyo Jozo KK
Priority to JP9839179A priority Critical patent/JPS5621599A/en
Publication of JPS5621599A publication Critical patent/JPS5621599A/en
Publication of JPS6140400B2 publication Critical patent/JPS6140400B2/ja
Granted legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To determine phosphoric acid in biospecimen such as serum, urine, etc., other phosphoric acid-containing specimen, or a phosphoric acid-producing reaction system, by reacting the specimen with a system containing pyruvic acid and pyruvate oxidase, and measuring the produced H2O2 or consumed O2.
CONSTITUTION: The objective kit is composed of (A) pyruvate oxidase obtained from the cells of Pediococcus, etc. and (B) pyruvic acid, and optionally (C) flavine- adenine dinucleotide (FAD), thiamine pyrophosphate, and calcium ions, etc. e.g. one or more ion-generating salts selected from e.g. CaCl2, CoCl2, MnCl2, etc. A necessary amount of the reaction liquid is taken out from the kit, and made to react with a specimen at 20W40°C for 5W60min. The amount of the phosphoric acid in the specimen can be determined either by determining the oxygen consumption shown in the formula, or by determining the produced H2O2 by colorimetry or fluorometry using 4-aminoantipyrine and phenol or N,N-dimethylaniline, homovanillic acid, etc. as an indicator. The method is useful not only for the determination of phosphoric acid or a phsophoric acid-producing system, but also for the deterination of phosphatase activity, fatty acid, or a component necessary for the examina tion of biospecimen which generates phosphoric acid by the reaction.
COPYRIGHT: (C)1981,JPO&Japio
JP9839179A 1979-07-31 1979-07-31 Kit for determination of phosphoric acid using pyruvate oxidase, and method for determination using the same Granted JPS5621599A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP9839179A JPS5621599A (en) 1979-07-31 1979-07-31 Kit for determination of phosphoric acid using pyruvate oxidase, and method for determination using the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP9839179A JPS5621599A (en) 1979-07-31 1979-07-31 Kit for determination of phosphoric acid using pyruvate oxidase, and method for determination using the same

Publications (2)

Publication Number Publication Date
JPS5621599A true JPS5621599A (en) 1981-02-28
JPS6140400B2 JPS6140400B2 (en) 1986-09-09

Family

ID=14218537

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9839179A Granted JPS5621599A (en) 1979-07-31 1979-07-31 Kit for determination of phosphoric acid using pyruvate oxidase, and method for determination using the same

Country Status (1)

Country Link
JP (1) JPS5621599A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6265179B1 (en) 2000-02-01 2001-07-24 Molecular Probes, Inc. Detection of phosphate using coupled enzymatic reactions

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6265179B1 (en) 2000-02-01 2001-07-24 Molecular Probes, Inc. Detection of phosphate using coupled enzymatic reactions

Also Published As

Publication number Publication date
JPS6140400B2 (en) 1986-09-09

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