JPS56106153A - Method for determination of alpha1-microglobulin by enzyme immunity measurement method - Google Patents

Method for determination of alpha1-microglobulin by enzyme immunity measurement method

Info

Publication number
JPS56106153A
JPS56106153A JP824980A JP824980A JPS56106153A JP S56106153 A JPS56106153 A JP S56106153A JP 824980 A JP824980 A JP 824980A JP 824980 A JP824980 A JP 824980A JP S56106153 A JPS56106153 A JP S56106153A
Authority
JP
Japan
Prior art keywords
alpha1
microglobulin
human
beads
antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP824980A
Other languages
Japanese (ja)
Inventor
Yoshihiro Koyamaishi
Kanzo Maeda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
FUJI ZOKI SEIYAKU
Fujirebio Inc
Original Assignee
FUJI ZOKI SEIYAKU
Fujirebio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by FUJI ZOKI SEIYAKU, Fujirebio Inc filed Critical FUJI ZOKI SEIYAKU
Priority to JP824980A priority Critical patent/JPS56106153A/en
Publication of JPS56106153A publication Critical patent/JPS56106153A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To perform highly accurate measurement with ease by using polystyrene beads bonded with anti-human-alpha1-microglobulin alpha-1-microglobulin antibody and anti-human-alpha1-microglobulin antibody marked with peroxidase. CONSTITUTION:Polystyrene beads are immersed in the anti-human-alpha1-microglobulin antibody soln. obtd. by applying a general method for separating and refining antibody protein to antiserums, whereby anti-human-alpha1-microglobulin antibody bond polystyrene beads are formed. Said polystyrene beads are immersed in a test tube contg. the liquid to be tested so that the alpha1-microglobulin (antigen) in the liquid to be tested is bound to the anti-human-alpha1-microglobulin insolubilized and stuck on the beads, after which the beads are washed and are immersed in a separately prepd. anti-human-alpha1-microglobulin antibody soln marked with peroxidase, so that the anti-human-alpha1-microglobulin antibody marked with peroxidase is bound to the antigen bound to the anti-human-alpha1-microglobulin antibody on the beads. Thence, the beads are washed and are immersed in an enzyme substrate soln. to allow the beads to form a color developement, and the absorbance thereof is measured, whereby the antigen is determined.
JP824980A 1980-01-29 1980-01-29 Method for determination of alpha1-microglobulin by enzyme immunity measurement method Pending JPS56106153A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP824980A JPS56106153A (en) 1980-01-29 1980-01-29 Method for determination of alpha1-microglobulin by enzyme immunity measurement method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP824980A JPS56106153A (en) 1980-01-29 1980-01-29 Method for determination of alpha1-microglobulin by enzyme immunity measurement method

Publications (1)

Publication Number Publication Date
JPS56106153A true JPS56106153A (en) 1981-08-24

Family

ID=11687858

Family Applications (1)

Application Number Title Priority Date Filing Date
JP824980A Pending JPS56106153A (en) 1980-01-29 1980-01-29 Method for determination of alpha1-microglobulin by enzyme immunity measurement method

Country Status (1)

Country Link
JP (1) JPS56106153A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6153192A (en) * 1990-08-06 2000-11-28 Boehringer Mannheim Gmbh Peptides with a characteristic antigenic determinant of α1-microglobulin
JP2018081027A (en) * 2016-11-17 2018-05-24 新日本無線株式会社 Method for modifying surface of electrode for biosensor

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6153192A (en) * 1990-08-06 2000-11-28 Boehringer Mannheim Gmbh Peptides with a characteristic antigenic determinant of α1-microglobulin
JP2018081027A (en) * 2016-11-17 2018-05-24 新日本無線株式会社 Method for modifying surface of electrode for biosensor

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