FR2273280A1 - Measurement of antigens and antibodies - in biological fluids by immuno diffusion techniques with antibodies marked with enzymes - Google Patents

Measurement of antigens and antibodies - in biological fluids by immuno diffusion techniques with antibodies marked with enzymes

Info

Publication number
FR2273280A1
FR2273280A1 FR7419100A FR7419100A FR2273280A1 FR 2273280 A1 FR2273280 A1 FR 2273280A1 FR 7419100 A FR7419100 A FR 7419100A FR 7419100 A FR7419100 A FR 7419100A FR 2273280 A1 FR2273280 A1 FR 2273280A1
Authority
FR
France
Prior art keywords
antibodies
antigens
biological fluids
enzyme
enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7419100A
Other languages
French (fr)
Other versions
FR2273280B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bpifrance Financement SA
Original Assignee
Agence National de Valorisation de la Recherche ANVAR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agence National de Valorisation de la Recherche ANVAR filed Critical Agence National de Valorisation de la Recherche ANVAR
Priority to FR7419100A priority Critical patent/FR2273280A1/en
Publication of FR2273280A1 publication Critical patent/FR2273280A1/en
Application granted granted Critical
Publication of FR2273280B1 publication Critical patent/FR2273280B1/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/559—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody through a gel, e.g. Ouchterlony technique

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Low concns. of antigens are measured by marking the antibodies with a stable enzyme of high specific activity which retains the major part of its activity after coupling, such as glucose oxidase or alkaline phosphatase, the enzyme being coupled to the antibody with a bridging agents such as glutaraldehyde, and the coupled enzyme is coloured on a plate, the plates then being used to measure antigen concn. corresponding to the marked antibody, by direct or indirect gel immunodiffusion techniques. Method is used in the testing of biological fluids such as cerebrospinal fluid, saliva, urine, intestinal liq., serum etc. The procedure is more sensitive that known immunodiffusion methods.
FR7419100A 1974-05-31 1974-05-31 Measurement of antigens and antibodies - in biological fluids by immuno diffusion techniques with antibodies marked with enzymes Granted FR2273280A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7419100A FR2273280A1 (en) 1974-05-31 1974-05-31 Measurement of antigens and antibodies - in biological fluids by immuno diffusion techniques with antibodies marked with enzymes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7419100A FR2273280A1 (en) 1974-05-31 1974-05-31 Measurement of antigens and antibodies - in biological fluids by immuno diffusion techniques with antibodies marked with enzymes

Publications (2)

Publication Number Publication Date
FR2273280A1 true FR2273280A1 (en) 1975-12-26
FR2273280B1 FR2273280B1 (en) 1976-12-24

Family

ID=9139551

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7419100A Granted FR2273280A1 (en) 1974-05-31 1974-05-31 Measurement of antigens and antibodies - in biological fluids by immuno diffusion techniques with antibodies marked with enzymes

Country Status (1)

Country Link
FR (1) FR2273280A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2380553A1 (en) * 1977-02-09 1978-09-08 Hidematsu Hirai METHOD OF DETECTION OF ANTIGENIC SUBSTANCES AND MATERIAL FOR THE DETECTION
EP0167340A2 (en) * 1984-06-26 1986-01-08 Kyowa Medex Co. Ltd. Method for assaying a biocomponent
EP0281477A1 (en) * 1987-03-03 1988-09-07 Biosys S.A. Method for the detection and counting of particulate antigens in a liquid sample, notably Clostridium tyrobutyricum spores in milk

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2380553A1 (en) * 1977-02-09 1978-09-08 Hidematsu Hirai METHOD OF DETECTION OF ANTIGENIC SUBSTANCES AND MATERIAL FOR THE DETECTION
EP0167340A2 (en) * 1984-06-26 1986-01-08 Kyowa Medex Co. Ltd. Method for assaying a biocomponent
EP0167340A3 (en) * 1984-06-26 1988-11-17 Kyowa Medex Co. Ltd. Method for assaying a biocomponent
EP0281477A1 (en) * 1987-03-03 1988-09-07 Biosys S.A. Method for the detection and counting of particulate antigens in a liquid sample, notably Clostridium tyrobutyricum spores in milk
WO1988006734A1 (en) * 1987-03-03 1988-09-07 Biosys S.A. Qualitative and quantitative determination of particulate antigens, in particular spores of clostridium tyrobutyricum
FR2611912A1 (en) * 1987-03-03 1988-09-09 Biosys Sa METHOD FOR DETECTION AND DETECTION OF PARTICULATE ANTIGENS IN A LIQUID SAMPLE, IN PARTICULAR CLOSTRIDIUM TYROBUTYRICUM SPORES IN MILK

Also Published As

Publication number Publication date
FR2273280B1 (en) 1976-12-24

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