JPS55129745A - Enzyme electrode - Google Patents

Enzyme electrode

Info

Publication number
JPS55129745A
JPS55129745A JP3815779A JP3815779A JPS55129745A JP S55129745 A JPS55129745 A JP S55129745A JP 3815779 A JP3815779 A JP 3815779A JP 3815779 A JP3815779 A JP 3815779A JP S55129745 A JPS55129745 A JP S55129745A
Authority
JP
Japan
Prior art keywords
enzyme
electrode
substance
substrate
immobilization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP3815779A
Other languages
Japanese (ja)
Other versions
JPS6116020B2 (en
Inventor
Shiro Nankai
Kenichi Nakamura
Takashi Iijima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Panasonic Holdings Corp
Original Assignee
Matsushita Electric Industrial Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Matsushita Electric Industrial Co Ltd filed Critical Matsushita Electric Industrial Co Ltd
Priority to JP3815779A priority Critical patent/JPS55129745A/en
Publication of JPS55129745A publication Critical patent/JPS55129745A/en
Publication of JPS6116020B2 publication Critical patent/JPS6116020B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To obtain the enzyme electrode of arbitrary shape, easy for enzyme immobilization, high in activity, and possible for continuous and repetitive usage, by using the film forming substance scattering conductive substance and immobilizing enzyme on the conductive film formed on the substrate surface.
CONSTITUTION: The conductive film 3 scattering carbon, noble metal, metal carbide or conductive metal oxide in the film forming substance such as alkali metal silicate, synthetic resin and rubber, is formed on the surface enclosing the platinum lead 2 at one end of the substrate 1 such as glass tube. The substance having amino base such as albumin is mixed on the film 3 in advance for easy cross-link immobilization of enzyme. The enzyme is immobilized on the layer 3 by using glutaraldehyde, and the enzyme electrode 10 is made as the immobilization enzyme layer 4. The electrode 10 is immersed in the buffer solution 11 including the substance specifically reacted with enzyme and the concentration of substrate is measured by using the unit consisting of the reference electrode 7, salt bridge 8, pair electrode 9, potentiostat 6 and recorder 5. Thus, the analysis of substrate can be made by using only slight and expensive enzyme.
COPYRIGHT: (C)1980,JPO&Japio
JP3815779A 1979-03-29 1979-03-29 Enzyme electrode Granted JPS55129745A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP3815779A JPS55129745A (en) 1979-03-29 1979-03-29 Enzyme electrode

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP3815779A JPS55129745A (en) 1979-03-29 1979-03-29 Enzyme electrode

Publications (2)

Publication Number Publication Date
JPS55129745A true JPS55129745A (en) 1980-10-07
JPS6116020B2 JPS6116020B2 (en) 1986-04-26

Family

ID=12517566

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3815779A Granted JPS55129745A (en) 1979-03-29 1979-03-29 Enzyme electrode

Country Status (1)

Country Link
JP (1) JPS55129745A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63222256A (en) * 1987-03-12 1988-09-16 Kokuritsu Shintai Shiyougaishiya Rihabiriteeshiyon Center Immobilization of biofunctional substance and electrode using the same
JPS63307350A (en) * 1987-05-20 1988-12-15 ケンブリッジ　ライフ　サイエンシズ　ピーエルシー Enzyme electrode
JPS6432160A (en) * 1987-07-29 1989-02-02 Terumo Corp Enzyme sensor and production thereof

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63222256A (en) * 1987-03-12 1988-09-16 Kokuritsu Shintai Shiyougaishiya Rihabiriteeshiyon Center Immobilization of biofunctional substance and electrode using the same
JPS63307350A (en) * 1987-05-20 1988-12-15 ケンブリッジ　ライフ　サイエンシズ　ピーエルシー Enzyme electrode
JPS6432160A (en) * 1987-07-29 1989-02-02 Terumo Corp Enzyme sensor and production thereof
JPH0583149B2 (en) * 1987-07-29 1993-11-24 Terumo Corp

Also Published As

Publication number Publication date
JPS6116020B2 (en) 1986-04-26

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