JPS57106850A - Measuring method of material concentration and enzyme electrode for its practice - Google Patents

Measuring method of material concentration and enzyme electrode for its practice

Info

Publication number
JPS57106850A
JPS57106850A JP55181843A JP18184380A JPS57106850A JP S57106850 A JPS57106850 A JP S57106850A JP 55181843 A JP55181843 A JP 55181843A JP 18184380 A JP18184380 A JP 18184380A JP S57106850 A JPS57106850 A JP S57106850A
Authority
JP
Japan
Prior art keywords
electrode
enzyme
film
immobilized
conc
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP55181843A
Other languages
Japanese (ja)
Inventor
Masao Koyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toshiba Corp
Original Assignee
Toshiba Corp
Tokyo Shibaura Electric Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toshiba Corp, Tokyo Shibaura Electric Co Ltd filed Critical Toshiba Corp
Priority to JP55181843A priority Critical patent/JPS57106850A/en
Publication of JPS57106850A publication Critical patent/JPS57106850A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes

Abstract

PURPOSE:To measure quickly conc. of substrates n sample soln. by measuring electric resistance or electric conductivity of carriers of immobilized enzyme. CONSTITUTION:An insulating material 4 is provided between the electrodes which are provided with a cylindrical electrode 2 having 6mm.phi outside diameter and rod shape electrode having 3mm.phi outside diameter. And, on the end face of lower part of electrode 2 and 3, 30mum thickness immobilized urease film 5, of which carrier is made of cellulose triacetate, is disposed and are fixed with sealing material 6. Further, as said film 5, the film, in which reactant consisting of polyatomic amine and glutanic aldehyde are contd. in the cellulose triacetate and the enzyme (urease) is immobilized chemicaly by utilizing the polar radical caused by above-mentioned compounds, is used. The enzyme electrode formed by such a manner is immersed in buffer soln. of phosphate and, thereby, for example, the conc. of urea having 10<-3>-10<-1>M range are mesured in a short time (within 1min) by such electrode.
JP55181843A 1980-12-24 1980-12-24 Measuring method of material concentration and enzyme electrode for its practice Pending JPS57106850A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55181843A JPS57106850A (en) 1980-12-24 1980-12-24 Measuring method of material concentration and enzyme electrode for its practice

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55181843A JPS57106850A (en) 1980-12-24 1980-12-24 Measuring method of material concentration and enzyme electrode for its practice

Publications (1)

Publication Number Publication Date
JPS57106850A true JPS57106850A (en) 1982-07-02

Family

ID=16107779

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55181843A Pending JPS57106850A (en) 1980-12-24 1980-12-24 Measuring method of material concentration and enzyme electrode for its practice

Country Status (1)

Country Link
JP (1) JPS57106850A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1984003562A1 (en) * 1983-03-11 1984-09-13 Matsushita Electric Ind Co Ltd Biosensor
JPS6150262U (en) * 1984-09-05 1986-04-04
EP0437789A2 (en) * 1990-01-19 1991-07-24 Gambro Ab A system, respectively a method, for measuring the concentration of a substance in a solution or dispersion thereof, whereby the substance consists of a compound which is non-electrically conducting or very slightly conducting
JP2007010321A (en) * 2005-06-28 2007-01-18 Sony Corp Biosensor

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1984003562A1 (en) * 1983-03-11 1984-09-13 Matsushita Electric Ind Co Ltd Biosensor
JPS6150262U (en) * 1984-09-05 1986-04-04
EP0437789A2 (en) * 1990-01-19 1991-07-24 Gambro Ab A system, respectively a method, for measuring the concentration of a substance in a solution or dispersion thereof, whereby the substance consists of a compound which is non-electrically conducting or very slightly conducting
EP0437789A3 (en) * 1990-01-19 1992-06-24 Gambro Ab A system, respectively a method, for measuring the concentration of a substance in a solution or dispersion thereof, whereby the substance consists of a compound which is non-electrically conducting or very slightly conducting
JP2007010321A (en) * 2005-06-28 2007-01-18 Sony Corp Biosensor
JP4696723B2 (en) * 2005-06-28 2011-06-08 ソニー株式会社 Biosensor

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