JPS55120519A - Protease inhibiting substance-2 and its preparation - Google Patents

Protease inhibiting substance-2 and its preparation

Info

Publication number
JPS55120519A
JPS55120519A JP2669679A JP2669679A JPS55120519A JP S55120519 A JPS55120519 A JP S55120519A JP 2669679 A JP2669679 A JP 2669679A JP 2669679 A JP2669679 A JP 2669679A JP S55120519 A JPS55120519 A JP S55120519A
Authority
JP
Japan
Prior art keywords
protein
give
molecular weight
fraction
inhibiting substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2669679A
Other languages
Japanese (ja)
Other versions
JPS6410520B2 (en
Inventor
Ikunori Koshiyama
Danji Fukushima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NODA SANGYO KAGAKU KENKYUSHO
Original Assignee
NODA SANGYO KAGAKU KENKYUSHO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by NODA SANGYO KAGAKU KENKYUSHO filed Critical NODA SANGYO KAGAKU KENKYUSHO
Priority to JP2669679A priority Critical patent/JPS55120519A/en
Publication of JPS55120519A publication Critical patent/JPS55120519A/en
Publication of JPS6410520B2 publication Critical patent/JPS6410520B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Peptides Or Proteins (AREA)

Abstract

NEW MATERIAL:A protase inhibiting substance-II only inhibiting trypsin, stable in PH of 3W10 at a temperature below 70°C. A molecular weight, 28,500. An isolectric point, PH 4.65. A sedimentation constant (S°20, W), 2.68S. A diffusion constant (D 20, W), 9.21×10-7cm2/sec. Elemental analysis: C, 42.35wt%; H, 6.85wt%; N, 12.26wt.
USE: A biochemical reagent. A drug for acute pancreatitis.
PROCESS: Water or a hydrochloric acid solution is added to soybeans or defatted soybeans or their powder and the prepared product is adjust to PH or 5.4W5.8 to extract protein. The extracted liquid is precipitated isoelectrically to give a sedimentary protein, which undergoes gel filtration or ultrafiltration so that a high-molecular weight protein fraction is removed from the protein. The prepared 2S fraction is adsorbed on an anion exchanger and the resulting product is subjected to fractionation by the concentration difference of salt to give the desired compound.
COPYRIGHT: (C)1980,JPO&Japio
JP2669679A 1979-03-09 1979-03-09 Protease inhibiting substance-2 and its preparation Granted JPS55120519A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2669679A JPS55120519A (en) 1979-03-09 1979-03-09 Protease inhibiting substance-2 and its preparation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2669679A JPS55120519A (en) 1979-03-09 1979-03-09 Protease inhibiting substance-2 and its preparation

Publications (2)

Publication Number Publication Date
JPS55120519A true JPS55120519A (en) 1980-09-17
JPS6410520B2 JPS6410520B2 (en) 1989-02-22

Family

ID=12200545

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2669679A Granted JPS55120519A (en) 1979-03-09 1979-03-09 Protease inhibiting substance-2 and its preparation

Country Status (1)

Country Link
JP (1) JPS55120519A (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5329909A (en) * 1976-08-28 1978-03-20 Kirin Brewery Trypsin inhibitor and its preparation

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5329909A (en) * 1976-08-28 1978-03-20 Kirin Brewery Trypsin inhibitor and its preparation

Also Published As

Publication number Publication date
JPS6410520B2 (en) 1989-02-22

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