JPS55120519A - Protease inhibiting substance-2 and its preparation - Google Patents
Protease inhibiting substance-2 and its preparationInfo
- Publication number
- JPS55120519A JPS55120519A JP2669679A JP2669679A JPS55120519A JP S55120519 A JPS55120519 A JP S55120519A JP 2669679 A JP2669679 A JP 2669679A JP 2669679 A JP2669679 A JP 2669679A JP S55120519 A JPS55120519 A JP S55120519A
- Authority
- JP
- Japan
- Prior art keywords
- protein
- give
- molecular weight
- fraction
- inhibiting substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Plant Substances (AREA)
- Peptides Or Proteins (AREA)
Abstract
NEW MATERIAL:A protase inhibiting substance-II only inhibiting trypsin, stable in PH of 3W10 at a temperature below 70°C. A molecular weight, 28,500. An isolectric point, PH 4.65. A sedimentation constant (S°20, W), 2.68S. A diffusion constant (D 20, W), 9.21×10-7cm2/sec. Elemental analysis: C, 42.35wt%; H, 6.85wt%; N, 12.26wt.
USE: A biochemical reagent. A drug for acute pancreatitis.
PROCESS: Water or a hydrochloric acid solution is added to soybeans or defatted soybeans or their powder and the prepared product is adjust to PH or 5.4W5.8 to extract protein. The extracted liquid is precipitated isoelectrically to give a sedimentary protein, which undergoes gel filtration or ultrafiltration so that a high-molecular weight protein fraction is removed from the protein. The prepared 2S fraction is adsorbed on an anion exchanger and the resulting product is subjected to fractionation by the concentration difference of salt to give the desired compound.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2669679A JPS55120519A (en) | 1979-03-09 | 1979-03-09 | Protease inhibiting substance-2 and its preparation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2669679A JPS55120519A (en) | 1979-03-09 | 1979-03-09 | Protease inhibiting substance-2 and its preparation |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS55120519A true JPS55120519A (en) | 1980-09-17 |
JPS6410520B2 JPS6410520B2 (en) | 1989-02-22 |
Family
ID=12200545
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2669679A Granted JPS55120519A (en) | 1979-03-09 | 1979-03-09 | Protease inhibiting substance-2 and its preparation |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS55120519A (en) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5329909A (en) * | 1976-08-28 | 1978-03-20 | Kirin Brewery | Trypsin inhibitor and its preparation |
-
1979
- 1979-03-09 JP JP2669679A patent/JPS55120519A/en active Granted
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5329909A (en) * | 1976-08-28 | 1978-03-20 | Kirin Brewery | Trypsin inhibitor and its preparation |
Also Published As
Publication number | Publication date |
---|---|
JPS6410520B2 (en) | 1989-02-22 |
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