JPS55101050A - Simple dispensation measuring method for hemoglobin glucoside in blood - Google Patents

Simple dispensation measuring method for hemoglobin glucoside in blood

Info

Publication number
JPS55101050A
JPS55101050A JP707179A JP707179A JPS55101050A JP S55101050 A JPS55101050 A JP S55101050A JP 707179 A JP707179 A JP 707179A JP 707179 A JP707179 A JP 707179A JP S55101050 A JPS55101050 A JP S55101050A
Authority
JP
Japan
Prior art keywords
phosphoric
ion
eluate
ion density
acid buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP707179A
Other languages
Japanese (ja)
Inventor
Shoji Maruyama
Suehiro Hiyoshi
Yasumitsu Miyamoto
Toshisada Ishido
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SOGO SEIBUTSU IGAKU KENKYUSHO
SOGO SEIBUTSU IGAKU KENKYUSHO KK
Original Assignee
SOGO SEIBUTSU IGAKU KENKYUSHO
SOGO SEIBUTSU IGAKU KENKYUSHO KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SOGO SEIBUTSU IGAKU KENKYUSHO, SOGO SEIBUTSU IGAKU KENKYUSHO KK filed Critical SOGO SEIBUTSU IGAKU KENKYUSHO
Priority to JP707179A priority Critical patent/JPS55101050A/en
Publication of JPS55101050A publication Critical patent/JPS55101050A/en
Pending legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE: To make it possible to take a measurement easily in a short time through simple operation, by measuring the absorbance of an eluate, by dispensing a sample by three weak acid ion-exchange resin columns balanced by three kinds of buffer liquids with specific pH and ion density.
CONSTITUTION: Into columns 7W9 filled with weak acid ion-exchange resins 4W6 balanced by phosphoric-acid buffer liquid 1 of pH 6.5W6.7 and ion density 0.01W 0.5M, phosphoric-acid buffer liquid 2 of pH 6.7W6.9 and ion density 0.02W0.1M, and phosphoric-acid buffer liquid 3 of pH 6.0W8.0 and ion density 0.1W1.5M, the same quantity of hemolysis inspecting objective 13 is added. Mext, a photoelectric colorimetric measurement of the 410nm absorbance of eluate 10W12 obtained as an eluate from corresponding phosphoric-acid buffer liquids 1W3 is taken. As a result, the measurement time can be reduced to approximate one-tenth in comparison with conventional one and about 100 inspecting objectives can be measured at a time.
COPYRIGHT: (C)1980,JPO&Japio
JP707179A 1979-01-26 1979-01-26 Simple dispensation measuring method for hemoglobin glucoside in blood Pending JPS55101050A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP707179A JPS55101050A (en) 1979-01-26 1979-01-26 Simple dispensation measuring method for hemoglobin glucoside in blood

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP707179A JPS55101050A (en) 1979-01-26 1979-01-26 Simple dispensation measuring method for hemoglobin glucoside in blood

Publications (1)

Publication Number Publication Date
JPS55101050A true JPS55101050A (en) 1980-08-01

Family

ID=11655837

Family Applications (1)

Application Number Title Priority Date Filing Date
JP707179A Pending JPS55101050A (en) 1979-01-26 1979-01-26 Simple dispensation measuring method for hemoglobin glucoside in blood

Country Status (1)

Country Link
JP (1) JPS55101050A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5872055A (en) * 1981-10-26 1983-04-28 Sogo Seibutsu Igaku Kenkyusho:Kk Simple measuring method for hemoglobin a1 in blood
EP0183181A2 (en) * 1984-11-28 1986-06-04 CHEMICAL LABORATORIES S.r.l. Process for the analysis of haemoglobin Hba2
JPH01199166A (en) * 1988-02-04 1989-08-10 Nippon Chemiphar Co Ltd Method for simple and precise differential measurement of saccharified hemoglobin in blood

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5872055A (en) * 1981-10-26 1983-04-28 Sogo Seibutsu Igaku Kenkyusho:Kk Simple measuring method for hemoglobin a1 in blood
JPH0231350B2 (en) * 1981-10-26 1990-07-12 Sogo Seibutsu Igaku Kenkyusho Kk
EP0183181A2 (en) * 1984-11-28 1986-06-04 CHEMICAL LABORATORIES S.r.l. Process for the analysis of haemoglobin Hba2
JPH01199166A (en) * 1988-02-04 1989-08-10 Nippon Chemiphar Co Ltd Method for simple and precise differential measurement of saccharified hemoglobin in blood

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