JPS5497692A - Linear mannan - Google Patents
Linear mannanInfo
- Publication number
- JPS5497692A JPS5497692A JP421478A JP421478A JPS5497692A JP S5497692 A JPS5497692 A JP S5497692A JP 421478 A JP421478 A JP 421478A JP 421478 A JP421478 A JP 421478A JP S5497692 A JPS5497692 A JP S5497692A
- Authority
- JP
- Japan
- Prior art keywords
- water
- cells
- treatment
- dialyzed
- precipitated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
PURPOSE: To prepare linear mannan useful as an injection for treatment of malignant tumor, free of a phosphoric acid group and proteins and having excellent anti- tumor activity, by extracting saccharomyces yeast cells, and separating and purifying the extract.
CONSTITUTION: A saccharomyces yeast, e.g. Saccharomyces cerevisiae, etc., is cultured, and the cultured cells are taken out, e.g. by centrifugal separation, etc., and washed. The cells are extracted with hot water at 100W150°C, and the extract is dialyzed and concentrated; crude polysaccharide is precipitated by adding a water-soluble solvent, e.g. ethanol, etc., to the concentrate. The polysaccharide is treated with Fehling's solution, etc., subsequently with a strong acid-type ion exchange resin, thereafter neutralized with a base, e.g. sodium carbonate, etc., concentrated, and dialyzed in flowing water. After the protein-removing treatment, the product is precipitated in a water-soluble solvent and filtrated. The desired neutral linear mannan is prepared by a column chromatography treatment of the aobove products.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP421478A JPS5497692A (en) | 1978-01-20 | 1978-01-20 | Linear mannan |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP421478A JPS5497692A (en) | 1978-01-20 | 1978-01-20 | Linear mannan |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5497692A true JPS5497692A (en) | 1979-08-01 |
Family
ID=11578362
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP421478A Pending JPS5497692A (en) | 1978-01-20 | 1978-01-20 | Linear mannan |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5497692A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MD4216C1 (en) * | 2012-08-15 | 2013-11-30 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Strain of Saccharomyces cerevisiae yeast - producer of mannans |
-
1978
- 1978-01-20 JP JP421478A patent/JPS5497692A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MD4216C1 (en) * | 2012-08-15 | 2013-11-30 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Strain of Saccharomyces cerevisiae yeast - producer of mannans |
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