JPS54122715A - Separation and purification of factor inhibiting mutagenesis of mutagenic agent from cabbage juice - Google Patents

Separation and purification of factor inhibiting mutagenesis of mutagenic agent from cabbage juice

Info

Publication number
JPS54122715A
JPS54122715A JP2884078A JP2884078A JPS54122715A JP S54122715 A JPS54122715 A JP S54122715A JP 2884078 A JP2884078 A JP 2884078A JP 2884078 A JP2884078 A JP 2884078A JP S54122715 A JPS54122715 A JP S54122715A
Authority
JP
Japan
Prior art keywords
mutagenesis
factor
cellulose
cabbage juice
exchange cellulose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2884078A
Other languages
Japanese (ja)
Other versions
JPS603287B2 (en
Inventor
Kazuyoshi Morita
Tsuneo Kata
Tadashi Inoue
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kanebo Ltd
Original Assignee
Kanebo Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kanebo Ltd filed Critical Kanebo Ltd
Priority to JP53028840A priority Critical patent/JPS603287B2/en
Priority to US05/940,089 priority patent/US4191752A/en
Publication of JPS54122715A publication Critical patent/JPS54122715A/en
Publication of JPS603287B2 publication Critical patent/JPS603287B2/en
Expired legal-status Critical Current

Links

Landscapes

  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PURPOSE: To separate and purify a factor which inhibits the mutagenesis of mutagenic substances such as heat-decomposition product of amino acids, etc., by treating a supernatant liquid obtained by the ultra-centrifugation of cabbage juice, successively with an anion exchange cellulose, a cation exchange cellulose, and a molecular sieve.
CONSTITUTION: Cabbage juice is centrifuged to remove tissue fragments, and further ultra-centrifuged to obtain supernatant liquid. The liquid is made to contact with an anion exchange cellulose, e.g. DEAE-cellulose, etc., and the unadsorbed fraction is subjected to column chromatography using a cation exchange cellulose e.g. carboxymethylcellulose as adsorbent, and the active fraction eluted at low salt concentration is collected. After optional ultrafiltration to remove low molecular components (MW≤30,000), the active fraction is treated with a molecular sieve such as Sephacryl S-200, etc., to obtain the objective mutagenesis-inhibiting factor. The factor is a proteinous substance having characteristic absorptions at 280 nm and 404 nm, stable to heat and unstable with a protease, and having a molecular weight of about 48,000.
COPYRIGHT: (C)1979,JPO&Japio
JP53028840A 1978-03-09 1978-03-14 Method for isolating and purifying factors that inhibit mutagenicity of mutagenic substances from cabbage use Expired JPS603287B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP53028840A JPS603287B2 (en) 1978-03-14 1978-03-14 Method for isolating and purifying factors that inhibit mutagenicity of mutagenic substances from cabbage use
US05/940,089 US4191752A (en) 1978-03-09 1978-09-06 Isolation of anti-mutagenic factor from cabbage juice

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP53028840A JPS603287B2 (en) 1978-03-14 1978-03-14 Method for isolating and purifying factors that inhibit mutagenicity of mutagenic substances from cabbage use

Publications (2)

Publication Number Publication Date
JPS54122715A true JPS54122715A (en) 1979-09-22
JPS603287B2 JPS603287B2 (en) 1985-01-26

Family

ID=12259555

Family Applications (1)

Application Number Title Priority Date Filing Date
JP53028840A Expired JPS603287B2 (en) 1978-03-09 1978-03-14 Method for isolating and purifying factors that inhibit mutagenicity of mutagenic substances from cabbage use

Country Status (1)

Country Link
JP (1) JPS603287B2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4474771A (en) * 1979-11-26 1984-10-02 Kanebo Ltd. High-molecular-weight substance having desmutagenic activity extracted from the root of burdock, and process for separating and purifying said substance
JP2000041624A (en) * 1998-08-04 2000-02-15 Koba Kazuaki Dried food of cabbage juice and cooking of fish utilizing the same

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4474771A (en) * 1979-11-26 1984-10-02 Kanebo Ltd. High-molecular-weight substance having desmutagenic activity extracted from the root of burdock, and process for separating and purifying said substance
JP2000041624A (en) * 1998-08-04 2000-02-15 Koba Kazuaki Dried food of cabbage juice and cooking of fish utilizing the same

Also Published As

Publication number Publication date
JPS603287B2 (en) 1985-01-26

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