JPS54122715A - Separation and purification of factor inhibiting mutagenesis of mutagenic agent from cabbage juice - Google Patents
Separation and purification of factor inhibiting mutagenesis of mutagenic agent from cabbage juiceInfo
- Publication number
- JPS54122715A JPS54122715A JP2884078A JP2884078A JPS54122715A JP S54122715 A JPS54122715 A JP S54122715A JP 2884078 A JP2884078 A JP 2884078A JP 2884078 A JP2884078 A JP 2884078A JP S54122715 A JPS54122715 A JP S54122715A
- Authority
- JP
- Japan
- Prior art keywords
- mutagenesis
- factor
- cellulose
- cabbage juice
- exchange cellulose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Plant Substances (AREA)
- Peptides Or Proteins (AREA)
Abstract
PURPOSE: To separate and purify a factor which inhibits the mutagenesis of mutagenic substances such as heat-decomposition product of amino acids, etc., by treating a supernatant liquid obtained by the ultra-centrifugation of cabbage juice, successively with an anion exchange cellulose, a cation exchange cellulose, and a molecular sieve.
CONSTITUTION: Cabbage juice is centrifuged to remove tissue fragments, and further ultra-centrifuged to obtain supernatant liquid. The liquid is made to contact with an anion exchange cellulose, e.g. DEAE-cellulose, etc., and the unadsorbed fraction is subjected to column chromatography using a cation exchange cellulose e.g. carboxymethylcellulose as adsorbent, and the active fraction eluted at low salt concentration is collected. After optional ultrafiltration to remove low molecular components (MW≤30,000), the active fraction is treated with a molecular sieve such as Sephacryl S-200, etc., to obtain the objective mutagenesis-inhibiting factor. The factor is a proteinous substance having characteristic absorptions at 280 nm and 404 nm, stable to heat and unstable with a protease, and having a molecular weight of about 48,000.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53028840A JPS603287B2 (en) | 1978-03-14 | 1978-03-14 | Method for isolating and purifying factors that inhibit mutagenicity of mutagenic substances from cabbage use |
US05/940,089 US4191752A (en) | 1978-03-09 | 1978-09-06 | Isolation of anti-mutagenic factor from cabbage juice |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53028840A JPS603287B2 (en) | 1978-03-14 | 1978-03-14 | Method for isolating and purifying factors that inhibit mutagenicity of mutagenic substances from cabbage use |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS54122715A true JPS54122715A (en) | 1979-09-22 |
JPS603287B2 JPS603287B2 (en) | 1985-01-26 |
Family
ID=12259555
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP53028840A Expired JPS603287B2 (en) | 1978-03-09 | 1978-03-14 | Method for isolating and purifying factors that inhibit mutagenicity of mutagenic substances from cabbage use |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS603287B2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4474771A (en) * | 1979-11-26 | 1984-10-02 | Kanebo Ltd. | High-molecular-weight substance having desmutagenic activity extracted from the root of burdock, and process for separating and purifying said substance |
JP2000041624A (en) * | 1998-08-04 | 2000-02-15 | Koba Kazuaki | Dried food of cabbage juice and cooking of fish utilizing the same |
-
1978
- 1978-03-14 JP JP53028840A patent/JPS603287B2/en not_active Expired
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4474771A (en) * | 1979-11-26 | 1984-10-02 | Kanebo Ltd. | High-molecular-weight substance having desmutagenic activity extracted from the root of burdock, and process for separating and purifying said substance |
JP2000041624A (en) * | 1998-08-04 | 2000-02-15 | Koba Kazuaki | Dried food of cabbage juice and cooking of fish utilizing the same |
Also Published As
Publication number | Publication date |
---|---|
JPS603287B2 (en) | 1985-01-26 |
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