JPH1190184A - Method for collecting and recovering microorganism and hollow-fiber membrane module for collecting microorganism - Google Patents

Abstract

PROBLEM TO BE SOLVED: To improve recovery efficiency and concn. efficiency of treated water at the time of collecting and recovering the microorganism in water by providing a stage for passing water into a hollow part from the outer membrane face side of each hollow-fiber membrane constituting a hollow-fiber membrane bundle to collect microorganism on the outer membrane face and a stage for recovering the microorganism collected on the outer membrane face. SOLUTION: The open end of the hollow-fiber membrane bundle 1 of a hollow-fiber membrane module communicating with the hollow part of each membrane is supported and fixed with a potting agent 2, and the bundle 1 is arranged in a vessel member A. When microorganism is collected and recovered, a sample water is introduced into the module from a water inlet 4 and filled in a region 7. The supplied water is permeated through the membrane from the outer membrane face side into the hollow part, introduced into a region 6 and finally discharged from a water intake 3. After the water is passed for a specified time, the water retained in the region 7 is recovered, a vessel member B is detached to expose the bundle 1, the bundle is cleaned, the cleaning soln. and the recovered water from the region 7 are joined, and the collected material is recovered.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a hollow fiber membrane module useful for analyzing and identifying microorganisms existing in water, in particular, pathogenic protozoa such as Cryptosporidium, and a method for capturing microorganisms using the same. .

[0002]

2. Description of the Related Art Tap water often has a bad smell of mold and mold due to contamination of a water source year by year, and its taste as water is often remarkably reduced. In recent years, as a further problem,
Cryptosporidium, a protozoan parasitic on cattle, pigs, etc., is often mixed in tap water, causing large numbers of infected people. It is said that Cryptosporidium is mixed into tap water because sewage from livestock farmers flows into surface water and contaminates the water source of tap water, but it is not clear yet.

[0003] Cryptosporidium is resistant to chemicals because it is wrapped in a bale-shaped membrane called oocysts having a major axis of 7 to 8 µm and a minor axis of 5 to 6 µm. It is very difficult to completely remove the water at the water treatment plant. The oocyst contains four sporosoids, and the oocysts ingested into the body, the sporosoids are cysted in the intestine and parasitized on the intestinal lining. The main symptom in infected individuals is watery diarrhea, which can lead to death in infected individuals with weak immunity.

The outline of a conventional method for analyzing Cryptosporidium is described below. (1) Collection process: 1 for raw tap water such as river surface water
A sample of 10 to 1400 liters is used as a membrane filter (absolute pore size 1 to 140 liters).
３3 μm) or a 10-inch wound spool cartridge (nominal 1 μm) to collect on the membrane surface. At this time, the filtration flow rate must not exceed 4 liters / minute. (2) Induction step: A 10-inch wound spool cartridge is divided into three parts, each of which is washed by hand in 1 liter of a washing liquid, and collected matter is collected. In the case of a membrane filter, it is recovered by washing or dissolving the filter. (3) Concentration and purification: Usually, a solution of sucrose, Tween 80, sodium dodecyl sulfate, and potassium citrate is used, and concentrated and purified by centrifugation using a density gradient. (4) Detection: The concentrated and purified sample is filtered through a membrane filter, and the oocyst is stained with an antibody by a direct or indirect fluorescent antibody method. This is inspected with an epifluorescence microscope and identified from the size and shape.

[0005]

In the above-mentioned conventional detection method, the recovery efficiency in all the processing steps is low, and the detection error is 55% when judged as negative and 18% when judged as positive. It is very large and is pointed out as a problem to be improved. These problems are considered to be mainly due to the following points. Oocysts are likely to remain on the membrane surface during collection / recruitment, and the collection efficiency is reduced. In particular, in the case of a thread wound cartridge, since the supplement enters the inside, the reduction in the collection rate tends to be particularly remarkable. A large amount of washing liquid is required to collect the microorganisms captured on the membrane surface, and the concentration rate is low. Skilled techniques are required in the derivation process and the detection process, but there is a shortage of skilled inspection technicians.

Further, in the above-mentioned method, the membrane surface often becomes clogged before sufficient water is passed through the sample. Especially,
In the case of a membrane filter, since the membrane area cannot be increased, there is also a problem in the process efficiency that clogging occurs with a flow rate of 10 liters or more.

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems in the conventional method, and greatly improves the recovery efficiency, the concentration efficiency of treated water, and the amount of treated water to increase the detection sensitivity, An object of the present invention is to provide a microorganism capturing method capable of reducing errors and a hollow fiber module for capturing microorganisms used in the method.

[0008] Another object of the present invention is to provide a microorganism capable of simplifying the operation of capturing microorganisms to be detected on the membrane surface and recovering the microorganisms from the membrane surface without relying on skilled techniques. An object of the present invention is to provide a capturing method and a hollow fiber membrane module for capturing microorganisms used in the capturing method.

[0009]

The method for capturing and recovering microorganisms according to the present invention is directed to a method for capturing and recovering microorganisms existing in water, wherein the method comprises the steps of: The method is characterized by comprising a step of capturing microorganisms on the outer membrane surface by passing water, and a step of collecting microorganisms captured on the outer membrane surface.

[0010] Further, the hollow fiber membrane module for capturing microorganisms of the present invention is a hollow fiber membrane module for capturing microorganisms from water, wherein the hollow fiber membrane module has a water supply port and a water intake port.
A hollow fiber membrane bundle, a fixing member for fixing the open end of the hollow fiber membrane constituting the hollow fiber membrane bundle while maintaining the open state, and communicating with the hollow portion through the open end of the hollow fiber membrane, and An intake-port side region that also communicates with the intake port, a water-supply-side region that is in contact with an outer membrane surface that forms a microbe capturing surface of the hollow fiber membrane, and that communicates with the water-supply port is provided. The water supply side area is divided, and the outer membrane surface is exposed by making the container portion covering the outer membrane surface forming the microorganism capturing surface of the hollow fiber membrane bundle detachable.

According to the method of the present invention, since the hollow fiber membrane is used for the filter, the hollow fiber membrane module can have a larger membrane area, for example, as compared with a thread wound cartridge of the same 10-inch size. Therefore, a large amount of sample water can be passed, and the concentration ratio of the sample water can be improved, so that even microorganisms existing at a very low concentration in water can be captured.

Further, by washing the hollow fiber membrane capturing the microorganisms on the outer membrane surface with a washing solution, the captured microorganisms can be easily collected from the outer surface of the hollow fiber into the washing solution. For example, the portion covering the hollow fiber membrane bundle of the hollow fiber membrane module is made detachable, this portion is removed during the washing operation, the hollow fiber membrane bundle is exposed, immersed in the cleaning solution, and captured by shaking. The microorganisms can be easily collected. At this time, the volume of the cleaning liquid is about three liters in the case of the thread wound cartridge, in which the cartridge is divided into three parts, and each of them requires about 3 liters of cleaning liquid for rinsing even in 1 liter of cleaning liquid. In the case of a module, treatment with about 1 liter of a cleaning solution is sufficient. That is, the washing efficiency can be increased and the amount of the washing solution can be reduced, and the burden on the centrifugation step after washing can be reduced. Furthermore, the collection efficiency can be improved by improving the concentration ratio by increasing the membrane area and improving the operability in the washing step and the like, and as a result, it becomes possible to capture microorganisms existing at a lower concentration.

The present invention is particularly useful, for example, for capturing and recovering microorganisms such as Cryptosporidium, which have a problem even if present in a trace amount in tap water.

[0014]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below with reference to the drawings. FIG. 1 is a sectional view schematically showing an example of a hollow fiber membrane module for capturing microorganisms according to the present invention.
The hollow fiber membrane bundle 1 in this hollow fiber membrane module is obtained by bending a large number of hollow fiber membranes into a U-shape and bundling one end (upper side in FIG. 1) so that each end is converged. The open end communicating with the hollow portion of each hollow fiber membrane is supported and fixed by a potting agent (fixing member) 2, whereby the hollow fiber membrane bundle 1 is arranged in the container member A. Further, the container member A
Has an intake 3 for taking out water from the region 6 to the outside of the container. The fixed state of the open ends of the hollow fiber membranes is as shown in FIG. 2, and the area 6 communicating with the hollow portion of each hollow fiber membrane via each open end, and the outer membrane surface of each hollow fiber membrane The contacting region 7 is separated by the potting agent 2, and the mass transfer between these regions is performed only through the surface of the hollow fiber membrane.

The container member B functions as a cover for the hollow fiber membrane bundle 1 and has a water supply port 4 for supplying sample water to the region 7. The container member B is detachably attached to the container member A.
-It is fitted by a fitting method such as a screw structure via a ring, so that the inside of the container can be hermetically sealed at portions other than the water intake port 3 and the water supply port 4.

The hollow fiber membrane constituting the hollow fiber membrane bundle 1 only needs to have a pore diameter capable of capturing microorganisms to be captured and permeable to water. For example, the hollow fiber membrane is used for water filtration. Various hydrophilic hollow fiber membranes can be used.
The hydrophilic hollow fiber membrane may be a hollow fiber membrane made of a hydrophilic material or a hollow fiber membrane obtained by hydrophilizing the surface of pores of a hollow fiber membrane made of a hydrophobic material by various methods.

Specific examples of the hydrophilic hollow fiber membrane include a porous polyethylene hollow fiber membrane having a surface coated with an ethylene-vinyl alcohol copolymer.

The selection of the pore size of the hollow fiber membrane is appropriately set according to the size of the microorganism to be captured. For example, in the capture of cryptosporidium, a hollow fiber membrane having a maximum pore size of 2 μm or less is preferably used. be able to. In other words, the oocyst of Cryptosporidium is 5μ even if it is small.
m, but the oocyst has some flexibility,
It has been reported that 7% passed through a 3 μm membrane filter, and a hollow fiber membrane having a maximum pore diameter of 2 μm or less is preferable for safety.

In the example of FIG. 1, the hollow fiber membrane is fixed with a potting agent in a state of being bent in a U-shape, but the method of fixing the hollow fiber membrane is not limited to this. For example, a method in which both open ends of the hollow fiber membrane are fixed so as to bridge between opposed fixing members while maintaining the open state, one end of the hollow fiber membrane is sealed, and the other end is opened. For example, a method of fixing to a fixing member can be adopted. In consideration of the operability and the like in the cleaning operation, a U-shaped focusing and fixing method as shown in FIG. 1 is preferable.

Further, the hollow fiber membrane bundle 1 more preferably has a hydrophilic portion and a hydrophobic portion. The hydrophobic portion, if necessary, present in the hollow fiber membrane bundle 1 prevents the gas in the sample water from permeating and prevents the gas from remaining on the outer membrane surface of the hollow fiber membrane to lower the filtration efficiency. ,
It is particularly preferably used when gas retention is likely to occur. The ratio of the hydrophobic portion to the entire hollow fiber membrane bundle is 1% to 20%.
% (The ratio of the volume of the hydrophobic portion to the total volume of all the hollow fiber membranes constituting the hollow fiber membrane bundle). If the amount is less than 1%, there is a case where the chance of contact with the gas is small and the effect of degassing by providing the hydrophobic portion cannot be sufficiently obtained, and if the amount is more than 20%, the flow rate of water decreases. There is. In order to provide a hydrophobic portion in the hollow fiber membrane bundle 1, for example, a method in which a hydrophilic hollow fiber membrane and a hydrophobic hollow fiber membrane are mixed and bundled, a method in which a hydrophobic hollow fiber membrane is partially hydrophilized to form a hydrophilic hollow fiber membrane, A method using this as a hollow fiber membrane having a fiber membrane portion and a hydrophobic hollow fiber membrane portion can be used.

Specific examples of the hydrophobic hollow fiber membrane include a hollow fiber membrane made of polyolefin such as polyethylene and polypropylene.

The filling rate of the hollow fiber membrane in the container is 10% to
It is preferably in the range of 50% (volume ratio), and if the filling factor is less than 10%, the volume of the module must be increased as a whole in order to obtain an effective effective membrane area and processing capacity. Therefore, the applicability to a case where a compact module is required is reduced. In addition, the filling rate is 50
%, The chance of contact between the hollow fiber membranes is reduced as much as possible, the effective membrane area is maintained without reduction, and the trapped matter captured by the hollow fiber membrane is more efficiently washed. Preferred for achieving a recovery operation.

The capture and recovery of microorganisms by the hollow fiber membrane module shown in FIG. 1 can be performed, for example, as follows. First, the sample water is introduced into the module from the water supply port 4 and filled in the area 7. The supplied water permeates into the hollow portion from the outer membrane surface side of the hydrophilic hollow fiber membrane, is guided to the region 6, and is finally taken out from the water inlet 3. The water flow pressure is set according to the configuration of the module to be used, for example, the pore diameter of the hollow fiber membrane, the effective membrane area provided by the hollow fiber membrane bundle, etc. For this purpose, for example, a pressure that allows water to flow at 1.0 kg / cm ² or less is preferable, and 0.5 kg / c 2
A pressure that allows water to flow at m ² or less is more preferable. When the water flow pressure exceeds 1.0 kg / cm ² , the trapped matter adheres to the surface of the hollow fiber membrane and cannot be completely washed, causing a decrease in the recovery rate. There is also.

When the water supply for a predetermined time is completed, the water remaining in the region 7 in the container is recovered, the container member B is removed to expose the hollow fiber membrane bundle, and the hollow fiber membrane bundle is washed. Collected matter can be collected by combining the collected water. The method of cleaning the hollow fiber membrane bundle may be any method that can collect the captured microorganisms and does not damage the captured microorganisms.The method may be such that the hollow fiber membrane bundle is immersed in a cleaning solution and stirred or shaken. A washing method, a method of pressing and washing with a glass rod or the like, a method of supplying a washing liquid from the water intake port 3 of the container member A and leaching the washing liquid to the outer membrane surface of the hollow fiber membrane to backwash, and a method of immersing the washing liquid in the washing liquid. A method of sonic cleaning can be used, and two or more of these methods can be combined. Water can be suitably used as the washing water.

The recovered liquid containing the captured microorganisms can be processed according to a conventional method including centrifugation and used for the intended analysis.

[0026]

The present invention will be described in more detail with reference to the following examples.

Reference Example 1 A high-density polyethylene (HB530 manufactured by Mitsubishi Chemical Corporation) was supplied to a hollow fiber manufacturing nozzle having a circular discharge port.
The yarn was spun at a discharge temperature of 180 ° C. and a take-up speed of 35 m / min to obtain a hollow fiber undrawn yarn. At this time, the spinning draft is 8
It was 6.

The undrawn hollow fiber thus obtained was heated at 125 ° C. for 12 hours.
After annealing for a time, stretch 1.25 times at room temperature,
Subsequently, in a heating furnace heated to 119 ° C., hot drawing was performed until the draw ratio from the undrawn yarn became 6 times, to obtain a hydrophobic porous hollow fiber membrane. The resulting hydrophobic porous hollow fiber membrane had an outer diameter of 380 μm and a thickness of 55 μm. When this hydrophobic porous hollow fiber membrane was observed with a scanning electron microscope, micropores of 0.4 to 0.5 μm were confirmed.

REFERENCE EXAMPLE 2 Sixteen hydrophobic porous hollow fiber membranes obtained in Reference Example 1 were combined and ethylene-vinyl alcohol copolymer (ethylene content 44 mol%, Nippon Synthetic Chemical Industry Co., Ltd.) maintained at 70 ° C. (Soarnol A, manufactured by Co., Ltd.) was immersed in a 1.3% by weight solution (solvent ethanol / water = 75/25; volume ratio) for 20 seconds, and then excessively adhered to the outer surface of the hydrophobic hollow fiber membrane by a ceramic guide. After squeezing the polymer solution,
It was started at a rising angle of 90 ° C. in an atmosphere having an ethanol vapor concentration of about 40% by volume, allowed to stay there for 80 seconds, and then dried with 55 ° C. hot air to obtain a hydrophilic fiber-treated hollow fiber membrane. The hydrophilization treatment is performed continuously through a series of the above steps, and the running speed of the hollow fiber membrane is 15 m / m.
min. The obtained hydrophilic hollow fiber membrane has a configuration in which a hydrophilic copolymer is coated on a hydrophobic material, and when this is observed with a scanning electron microscope, it is 0.6 to 0.7.
μm micropores were confirmed.

Example 1 A loop obtained by combining one hydrophobic porous hollow fiber membrane produced in Reference Example 1 and 16 hydrophilic porous hollow fiber membranes produced in Reference Example 2 was cut into a loop. A hollow fiber membrane module for capturing microorganisms having the configuration shown in FIG. 1 according to the following specifications was produced by a conventional method.

[0031]

[Table 1] When the river water of the Yada River flowing through Nagoya City in Aichi Prefecture was flowed at a flow rate of 4 L / min through this hollow fiber membrane module for capturing microorganisms, the water flow pressure loss increased to 0.5 kg / cm ² at an integrated flow rate of 800 L. Then, the water flow was stopped. At this time, the evaporation residue concentration of the Yada river water was 30 ppm. In addition, the concentration of the evaporation residue is measured by an ordinary method, and the same applies to the following Examples and Comparative Examples.

Next, the container members A and B (see FIG. 1) of the hollow fiber membrane module for capturing microorganisms are divided, water remaining in the container member B is recovered, and the hollow fiber membrane of the container member A is further reduced to 1 liter. After washing with water for washing for 5 minutes using a glass rod, the hollow fiber membrane was taken out of the washing water, and the outer membrane surface of the hollow fiber membrane was washed with 0.1 liter of fresh washing water. The retained water in the container member B was 0.4 liter, the total amount of the collected liquid combined with the cleaning liquid obtained by the two washing operations was 1.5 liter, and the concentration ratio was about 530 times. The concentration of the evaporated residue in the recovered liquid is about 15250 ppm, and the concentration of the evaporated residue in the recovered liquid calculated from the concentration of the evaporated residue in the Yada River water, the amount of water flow, and the amount of the recovered liquid is about 16000 ppm, so that it is 95% or more. It was confirmed that it was a recovery rate.

Example 2 In the same manner as in Example 1, a hollow fiber membrane module for capturing microorganisms was produced, and the river water of the Yada River was passed. that time,
When water flow was performed at a flow rate of 4 liters / minute, the water flow pressure loss increased to 0.5 kg / cm ² at an integrated flow rate of 800 liters, and water flow was stopped. At this time, the evaporation residue concentration of the Yada river water was 30 ppm.

Next, the container members A and B (see FIG. 1) of the hollow fiber membrane module for capturing microorganisms are divided, the water remaining in the container member B is recovered, and the washing water 1 is passed through the water inlet of the container member A. The liter is passed at a rate of 1 liter / minute in the reverse direction of the trapping water, and the glass fiber is used to wash the hollow fiber membrane in the washing liquid that has passed through the hollow fiber membrane for 5 minutes while passing water. did. After pulling up the hollow fiber membrane from the washing solution, the outer surface of the hollow fiber membrane was washed with 0.1 L of fresh washing water under running water. The retained water in the container member B was 0.4 liter, the total amount of the collected liquid combined with the cleaning liquid obtained by the two washing operations was 1.5 liter, and the concentration ratio was about 530 times. The concentration of the evaporation residue in the recovered liquid is about 15,800 ppm, and the concentration of the evaporation residue in the recovered liquid calculated from the evaporation residue concentration, the amount of water flow, and the amount of the recovered liquid is about 16,000 p.
pm, it was confirmed that the recovery was about 99%.

Example 3 In the same manner as in Example 1, a hollow fiber membrane module for capturing microorganisms was produced, and the water of the Yada River was passed. that time,
When water flow was performed at a flow rate of 4 liters / minute, the water flow pressure loss increased to 0.5 kg / cm ² at an integrated flow rate of 800 liters, and water flow was stopped. At this time, the evaporation residue concentration of the Yada river water was 30 ppm.

Next, the container members A and B (see FIG. 1) of the hollow fiber membrane module for capturing microorganisms are divided, water remaining in the container member B is recovered, and the hollow fiber membrane of the container member A is further reduced to 1 liter. Was subjected to ultrasonic cleaning for 5 minutes in an ultrasonic cleaning tank containing cleaning water. Thereafter, the hollow fiber membrane was pulled out of the ultrasonic cleaning tank, and the outer membrane surface of the hollow fiber membrane was washed with 0.1 L of fresh washing water. The retained water in the container member B was 0.4 liter, the total amount of the collected liquid combined with the cleaning liquid obtained by the two washing operations was 1.5 liter, and the concentration ratio was about 530 times. Evaporation residue concentration of the recovered liquid is about 15800 pp
m, and the concentration of the evaporation residue of the recovered liquid calculated from the concentration of the evaporation residue, the amount of water flow, and the amount of the recovered liquid is approximately 160
Since it was 00 ppm, it was confirmed that the recovery was about 99%.

Example 4 The procedure of Example 1 was repeated except that a hydrophilic hollow fiber membrane was used in place of the hydrophobic hollow fiber membrane. went. that time,
When water flow was performed at a flow rate of 4 liters / minute, the water flow pressure loss increased to 0.5 kg / cm ² at an integrated flow rate of 450 liters, and water flow was stopped. When the surface of the hollow fiber membrane was observed after the stop, bubbles were observed between the hollow fiber membranes. At this time, the evaporation residue concentration of the Yada river water was 30 ppm.

Next, in the same manner as in the third embodiment,
Collection of retained water in the inside, ultrasonic washing and running water washing were performed. The retained water in the container member B was 0.4 liter, and the total amount of the collected liquid including the cleaning liquid obtained by the two washing operations was 1.5 liters, and the concentration ratio was about 300 times. The concentration of the evaporation residue in the recovered liquid is about 8900 ppm, and the concentration of the evaporation residue in the recovered liquid calculated from the concentration of the evaporation residue in the Yada River water, the amount of water flow, and the amount of the recovered liquid is about 9000 ppm. It was confirmed that it was a recovery rate.

COMPARATIVE EXAMPLE 1 River water from the Yada River was passed through a 10-inch polypropylene thread wound cartridge having a nominal pore size of 1 μm at a flow rate of 4 liters / minute in the same manner as in Examples 1 to 4.
At the time of liter, the water pressure increased to 0.5 kg / cm ² , and the water flow was stopped. At this time, the evaporation residue concentration of the Yada river water was 30 ppm.

Next, the water remaining in the cartridge holder was collected, and the cartridge was further divided into three parts in the vertical direction, and each was washed with 1 liter of water for washing using a glass rod for 5 minutes. The amount of water retained in the cartridge holder was 1.7 liters, the total amount of the collected liquid combined with the cleaning liquid was 4.7 liters, and the concentration ratio was 43 times. The concentration of the evaporated residue in the recovered liquid is about 230 ppm, and the calculated value from the concentration of the evaporated residue in the Yada River water and the concentration ratio is about 2 ppm.
Since it was 75 ppm, it was confirmed that the recovery was about 85%.

As described above, according to the results of Examples 1 to 4 and Comparative Example 1, according to the method for capturing and recovering microorganisms using the hollow fiber membrane module for capturing microorganisms of the present invention, the recovery rate is 10 times lower than that of a 10-inch wound spool cartridge. It is clear that the percentage is improved by more than%. In addition, in the concentration ratio, when the hydrophobic hollow fiber membrane was mixed, a particularly remarkable effect was obtained as the concentration ratio was about 12 times as large as that of the 10-inch wound spool cartridge. Furthermore, it was found that the integrated flow rate was 2-3 times higher, and it was possible to capture microorganisms existing in water at lower concentrations. On the other hand, the total amount of the recovered liquid can be reduced to about 1/3, and the subsequent centrifugation step can be reduced.

As a method for cleaning the hollow fiber membrane, an ultrasonic cleaning method is preferable because the operation is simple and the recovery rate is high. However, when the ultrasonic treatment may have an adverse effect on the captured microorganisms, it is desirable to wash by push washing or back washing.

[0043]

According to the present invention, since a hollow fiber membrane is used as a membrane for capturing microorganisms, the membrane area can be increased and a large amount of sample water can be passed, so that the establishment of detection of microorganisms is improved. The derivation operation is also simplified, and the microorganisms can be captured and recovered at a high recovery rate. Further, since the washing operation can be simplified and the amount of the collected liquid can be reduced as compared with the conventional method, the burden on the laboratory technician can be reduced.

[Brief description of the drawings]

FIG. 1 is a schematic sectional view of an example of a hollow fiber membrane module for capturing microorganisms used in the present invention.

FIG. 2 is a partial cross-sectional view for explaining a fixed state of a hollow fiber membrane end of a hollow fiber membrane module.

[Explanation of symbols]

 REFERENCE SIGNS LIST 1 hollow fiber membrane bundle 2 potting agent 3 water inlet 4 water inlet 5 O-ring 6 water inlet side area 7 water inlet side area A hollow fiber membrane fixed side container member B container member constituting hollow fiber membrane cover

Claims (23)

[Claims] 1. A method for capturing and recovering microorganisms existing in water, wherein water is passed from the outer membrane surface side of each hollow fiber membrane constituting the hollow fiber membrane bundle into the hollow portion, so that the microorganisms are deposited on the outer membrane surface. A method for capturing and recovering microorganisms, comprising a step of capturing and a step of recovering microorganisms captured on the outer membrane surface. 2. The hollow fiber membrane is fixed to a fixing member while keeping its open end open, and the outer membrane surface of the hollow fiber membrane is connected to a region communicating with the hollow portion of the hollow fiber membrane. The method for capturing and recovering microorganisms according to claim 1, wherein the method is arranged in a hollow fiber membrane module in which a contact area is divided. 3. The method for capturing and recovering microorganisms according to claim 2, wherein the filling rate of the hollow fiber membrane into the module is in a range of 10 to 50%. 4. The method for capturing and recovering microorganisms according to claim 2, wherein the hollow fiber membrane bundle includes a hollow fiber membrane having one end closed and the other end fixed to the fixing member as an open end. . 5. The hollow fiber membrane bundle includes a hollow fiber membrane having both ends fixed to the fixing member as open ends.
Or the method of capturing and collecting microorganisms according to 3. 6. The hollow fiber membrane is bent into a U-shape to form the hollow fiber membrane bundle, and the open end of each hollow fiber membrane is fixed to one end of the hollow fiber membrane bundle by the fixing member. The method for capturing and recovering microorganisms according to claim 5, which is performed. 7. The method for collecting and capturing microorganisms according to claim 1, wherein a hollow fiber membrane portion for ventilation is mixed. 8. The method for capturing and recovering microorganisms according to claim 7, wherein the ratio of the hollow fiber membrane portion for ventilation in the hollow fiber bundle is in the range of 10 to 20%. 9. The method for capturing and recovering microorganisms according to claim 1, wherein the recovery of the microorganisms captured on the outer membrane surface of the hollow fiber membrane is performed by washing the hollow fiber membrane. 10. The method of capturing and recovering microorganisms according to claim 9, wherein the cleaning treatment is performed in a state where the hollow fiber membrane is immersed in a cleaning liquid. 11. The method for capturing and recovering microorganisms according to claim 9, wherein the cleaning treatment is performed by ultrasonic cleaning with the hollow fiber membrane immersed in a cleaning liquid. 12. The method for capturing and recovering microorganisms according to claim 9, wherein the washing treatment is performed by a back washing treatment in which water is passed from the inside of the hollow portion of the hollow fiber membrane toward the outer membrane surface. 13. The method for capturing and recovering microorganisms according to claim 1, wherein the maximum pore size of the hollow fiber membrane is 2 μm or less. 14. A pressure for passing water through the hollow fiber membrane is 1.0.
The method for capturing and recovering microorganisms according to any one of claims 1 to 13, wherein the weight is not more than kg / cm ² . 15. A hollow fiber membrane module for capturing microorganisms from water, comprising a hollow fiber membrane bundle and a hollow fiber membrane constituting the hollow fiber membrane bundle in a container having a water supply port and a water intake port. A fixing member for fixing the open end of the hollow fiber membrane while keeping the open state; a water intake side region communicating with the hollow part through the open end of the hollow fiber membrane, and also communicating with the water intake; A water supply side region that is in contact with the outer membrane surface that forms the trapping surface and communicates with the water supply port, wherein the water intake side region and the water supply side region are separated, and microorganisms in the hollow fiber membrane bundle are provided. A hollow fiber membrane module for capturing microorganisms, wherein the outer membrane surface is exposed by detachably attaching a container portion covering the outer membrane surface forming the capturing surface. 16. The hollow fiber membrane module for capturing microorganisms according to claim 15, wherein a filling rate of the hollow fiber membrane into the module is in a range of 10 to 50%. 17. The hollow fiber for capturing microorganisms according to claim 15, wherein the hollow fiber membrane bundle includes a hollow fiber membrane having one end closed and the other end fixed to the fixing member as an open end. Membrane module. 18. The hollow fiber membrane module for capturing microorganisms according to claim 15, wherein the hollow fiber membrane bundle includes a hollow fiber membrane having both ends fixed to the fixing member as open ends. 19. The hollow fiber membrane is folded in a U-shape to form the hollow fiber membrane bundle, and the open end of each hollow fiber membrane is fixed to one end of the hollow fiber membrane bundle by the fixing member. The hollow fiber membrane module for capturing microorganisms according to claim 18, which is provided. 20. The hollow fiber membrane module for capturing microorganisms according to any one of claims 15 to 19, wherein a hollow fiber membrane portion for ventilation is mixed. 21. The ratio of the hollow fiber membrane portion for ventilation in the hollow fiber bundle is in the range of 10 to 20%.
The hollow fiber membrane module for capturing microorganisms according to item 1. 22. A water passing pressure through the hollow fiber membrane is 1.0.
The hollow fiber membrane module microorganism capture according to any one of kg / cm ² or less is claims 15 to 21. 23. The hollow fiber membrane module for capturing microorganisms according to claim 15, wherein a maximum pore diameter of the hollow fiber membrane is 2 μm or less.