JPH1175832A - Microorganism mixture and degradation of oil and fat - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a mixture of photosynthetic bacteria, comprising Chromatium buderi, etc., and capable of efficiently degrading and removing oil and fat in a waste water in a grease trap without affecting the environment and a human body. SOLUTION: This mixture of photosynthetic bacteria is constituted of Chromatium buderi, Amoebobacter roseus, Rhodopseudomonas acidophila and Rhodopseudomonas capsula. An oil and fat in a waste water 11 in a grease trap 1 are preferably degraded and removed by adding a surfactant, a supplying source of a dissolved oxygen, a photosynthetic bacterium nutrient and the mixture of the photosynthetic bacteria as a pretreatment of the waste water 11 in the grease trap 1.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for effectively decomposing and removing fats and oils in wastewater in a grease trap without affecting the environment and the human body.

[0002]

2. Description of the Related Art A large amount of oil is used every day in a place having a commercial kitchen such as a restaurant, a hotel, a catering center, and a hospital. Therefore, the wastewater also contains a large amount of oil. The oils and fats contained in the drainage solidify below the freezing point, adhere to the drainage pipe, and cause clogging of the drainage pipe. In addition, if the wastewater is directly poured into a large-scale sewage treatment plant, it may exceed the treatment capacity of the sewage treatment plant and may further reduce the treatment capacity of the treatment plant. In addition, if wastewater containing oils and fats flows into a river, it causes pollution of the river.

[0003] In order to solve such a situation, the
In 1999, the Ministry of Construction Notification No. 1597 required the installation of a grease strap, which is a grease trap. That is, "A. When sewage contains oils, gasoline, earth and sand, and other substances that may significantly impair the function of piping facilities for drainage or damage the piping facilities for drainage,
Provide interceptors in effective locations. B. Grease from sewage,
A structure that can effectively separate gasoline, earth and sand, etc. Was required. Such a grease trap has an inflow port into which waste water flows from the kitchen and a drain port for discharging the waste water in the grease trap to a drain pipe or a septic tank, and the inside of the tank is divided into three to four layers to increase the residence time. This is a water storage tank having a structure in which fats and oils are trapped in the trap. However, although the grease trap has the function of separating or collecting oil from water, it does not have the ability to decompose oil, so it is necessary to periodically collect and remove the separated and accumulated grease from the grease trap. Is required.

[0004] As a method for collecting and removing oils and fats from wastewater, a method is employed in which a business operator scoops and collects coagulated oil manually, or entrusts a collecting company to collect oil with a vacuum machine. Was. The fats and oils collected by the collector are then disposed of at an industrial waste disposal site. However, industrial waste treatment plants nationwide have already reached the limit of their processing capacity, and construction of a new industrial waste treatment plant is difficult due to environmental impacts and opposition from the residents. For this reason, some companies have illegally dumped the collected oil into the ocean, and there has been a situation in which the necessity of installing a grease trap to prevent the pollution of rivers and eventually the ocean is rendered meaningless.

[0005] Wastewater containing oils and fats is suitable for propagation of various bacteria. For this reason, the grease trap easily becomes a hotbed of various bacteria, and some of the various bacteria have pathogenicity, which is problematic in terms of hygiene. In addition, odors caused by propagation of various bacteria and decay of wastewater in the grease trap are also problems.

For this reason, there is a demand for a method that not only separates and accumulates fats and oils with a grease trap but also decomposes fats and oils in waste water to lower the molecular weight.

[0007] Wastewater treatment methods can be broadly divided into two types: physicochemical treatment and biological treatment. When organic components in wastewater are to be treated, biological treatment is cheaper and more efficient than physicochemical treatment.

[0008]

SUMMARY OF THE INVENTION An object of the present invention is to provide a method for effectively decomposing and removing fats and oils in wastewater in a grease trap without affecting the environment and the human body.

[0009]

Means for Solving the Problems After diligent research, the present inventor has found a combination of microorganisms that efficiently decomposes and removes oils and fats in wastewater in a grease trap, and has completed the present invention. That is, in the present invention, the mixture of the photosynthetic bacteria of the present invention is Chromatium buderi, Amoebobacter roseus, Rhodop.
seudomonas acidophila, and Rhodopseudomonas cap
Provide a mixture of photosynthetic bacteria composed of sulata.
Furthermore, the present invention provides a method for decomposing and removing fats and oils in wastewater in a grease trap by supplying a mixture of the photosynthetic bacteria to wastewater in a grease trap. In this case, it is preferable to add a surfactant, a dissolved oxygen source, a photosynthetic bacteria nutrient, and a mixture of photosynthetic bacteria as pretreatment of the wastewater in the grease trap. Preferably, air is blown into the wastewater in the grease trap to make it aerobic, and at the same time, the fat and oil in the wastewater is decomposed while stirring the wastewater.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION The mixture of photosynthetic bacteria of the present invention is Ch
romatium buderi, Amoebobacter roseus, Rhodopseud
omonas acidophila, and Rhodopseudomonas capsula
consisting of ta. The former two are purple sulfur bacteria and the latter two are purple non-sulfur bacteria, both of which have excellent fat and oil decomposability.

The mixture ratio of each photosynthetic bacterium of the mixture of the present invention is in the range of all kinds being equal to either more or less, but is preferably 3: 2: 3: 2 to 2: 1: 1: 2, or It is contained in a ratio of 1: 1: 1: 0.5. The mixture of photosynthetic bacteria of the present invention may be solid or liquid. When the photosynthetic bacterium mixture of the present invention is a solid (dried cells), it is preferably used after being suspended in an appropriate liquid medium, buffer or the like. The mixture of the present invention contains the above photosynthetic bacteria at a certain ratio, when each microorganism alone decomposes oils and fats in the wastewater in the grease trap, and also, Chromatium buderi, Amoebobacter roseus, Rhod
opseudomonas acidophila, and Rhodopseudomonas c
It exerts an excellent action of decomposing fats and oils as compared to a state in which at least one of apsulata is missing.

Conventionally, when wastewater treatment is performed biologically, activated sludge is generally used. However, since the details of the microorganisms living in the activated sludge are unknown, the reproducibility of the treatment capacity is low, and And it is not possible to selectively decompose fats and oils. On the other hand, the mixture of the above specific microorganisms of the present invention has an extremely high ability to decompose fats and oils, has excellent reproducibility, and is easy to control.

When the microorganisms used in the present invention are used for treating fats and oils in wastewater, the fats and oils, that is, lipids are first hydrolyzed into fatty acids and glycerin. Fatty acids are decomposed to acetyl-CoA by β-oxidation, and acetyl-CoA is
Enter CA cycle. Glycerin is converted to glycerol 3-phosphate by glycerokinase and then to dihydroxyacetone phosphate by glycerol 3-phosphate dehydrogenase and enters the glycolysis system. Thus, the mixture of photosynthetic bacteria of the present invention can efficiently decompose and remove fats and oils.

The present invention also provides a method for supplying the mixture of the photosynthetic bacteria to wastewater in a grease trap to decompose and remove oils and fats in the wastewater.

Hereinafter, the present invention will be described in detail with reference to a conceptual diagram of an example of an embodiment of the present invention in FIG. In the treatment of the fats and oils in the drainage 11 according to the present invention, the purification liquid 25 is added to the drainage 11 flowing into the grease trap 1 from the kitchen by the purification liquid supply means 2 near the inflow port provided in the grease trap, and at the same time, The ventilation is performed by the ventilation means 3, and the wastewater 11 and the purifying liquid 25 are stirred in the grease trap, whereby the fats and oils in the wastewater 11 are decomposed by the photosynthetic bacteria in the purifying liquid 25. At this time, since the lower part of the grease trap is open and communicated as described above, the purifying liquid 25 is added, and the drainage 11 in the first tank 14 stirred by ventilation is further aerated and stirred in the second tank 15. Is done. The wastewater 11 aerated and agitated in the second tank 15 is further aerated and agitated in the third layer 16, flows out of the drain port, and is sent as treated water 13 to a sewer pipe or a purification tank.

The purifying liquid 25 filled in the tank 21 is sent by the operation of a pump 24, and impurities such as dust are removed by a strainer 22, and is added to drainage in a grease trap through a liquid sending line 23. You.

As the purifying liquid 25, a mixture (Solution D) of the photosynthetic bacteria of the present invention is used. Further, when the degree of contamination of the wastewater is severe, it is preferable to use a dissolved oxygen supply, open all (solution B) manufactured by Dutrel Laboratory. This addition also supplies minerals necessary for the growth of photosynthetic bacteria. In the case of further contamination, addition of a photosynthetic bacterial nutrient, preferably a mixture (solution C) of blood meal and BN bacteria (a product of Meiji Seika Co., Ltd.), which is a kind of Bacillus subtilis, and a surfactant (solution A) are also possible. Good. Further, when bacteria-derived protein accumulates, a protease, for example, an enzyme such as pepsin may be used.

When the solution D and the solution B, the solution C, the solution A, the enzyme and the like are used in combination, it is preferable that each solution is put in a separate tank. Therefore, in this case, the purified liquid supply means 2 having a plurality of tanks is used, or the plurality of purified liquid supply means 2 is used. When a plurality of types of purification liquids 25 are used, it is preferable that the liquid supply line 23 is also separate.

When the inflow and outflow of the wastewater is inactive, for example, it is preferable to add the purified water 25 in batches or in divided portions after the closing of business, because the treatment efficiency is high and it is preferable that the wastewater 11 flows into the grease trap for 24 hours. When the purification is performed continuously, the purification liquid 25 may be added in portions at appropriate time intervals. When only the D liquid is used as the purifying liquid 25,
Purification liquid 25 is added in an amount of 10 ml to 50 ml, preferably 10 ml, per day to 100 g of the grease trap. The number of operations and the operation time of these pumps 24 can be controlled by a timer.

Further, according to the present method, the inside of the grease trap 1 is periodically or irregularly observed after a certain period of time after the implementation, and the degree of aeration and / or photosynthesis is determined in accordance with the degree of decomposition of oil and fat in the grease strap 1. Bacterial mixture (D
Liquid), or at least one of the other liquids A, B, C or enzyme. By observing the inside of the grease trap 1 with a sensor and changing the degree of ventilation and / or the type of liquid and the amount of liquid supplied by an electric signal, the oil and fat in the drainage can be automatically automatically used for a long time by using the method of the present invention. Can be decomposed and removed.

Further, the air sent from the diagram from blower 31 is passed through the air supply line 32 and discharged from the diffuser pipe 33 by the ventilation means 3, whereby the drainage 11 in the green strap 1 is aerated and agitated.

The air supplied by the diamond from blower 31 is discharged through a number of holes provided in the air diffuser 33 through the air supply line 32 into the drainage in the grease trap, thereby uniformly discharging the drainage in the grease trap. Stir with aeration. As a result, the inside of the grease trap is maintained under aerobic conditions, the growth environment of the photosynthetic bacteria added by the purification solution supply means 2 is adjusted, and the oils and fats are agitated by aeration, emulsified and dispersed in the wastewater, whereby the photosynthesis is performed. Decomposition by bacteria becomes easy. In addition, propagation of anaerobic germs is suppressed, and generation of offensive odor is suppressed. For this reason, it is preferable that the ventilation means 3 is always operated even when the purification device supply means 2 is not operating. The ventilation rate is 10 L / 100 L / min to 40 L / 100 L / min, preferably 15 L / 100 L / min.
L / min to 25 L / 100 L / min. The present apparatus may have a plurality of ventilation means, and when a large amount of ventilation is used, it is preferable to use a plurality of ventilation means rather than to use a single ventilation means because more uniform ventilation and stirring can be achieved.

The grease strap to which the method of the present invention is applied is not particularly limited in terms of shape, number of tanks, and capacity.
It can be suitably used for a grease strap of about 00L to 500L.

The method according to the invention is preferably carried out after the pretreatment of the drainage in the grease trap. For the pretreatment, a starter kit which is a pretreatment kit composed of four kinds of liquids A to D is used.

Among the constituent liquids of the starter kit, the liquid A is a surfactant. Solution A eliminates bacteria that have survived in the grease trap. The liquid B is a dissolved oxygen supply source, preferably an open oar manufactured by Doutrell Laboratory, and supplies oxygen to wastewater to replenish minerals. Many of the bacteria growing in the grease trap before the treatment are anaerobic, and these bacteria can be killed by increasing the dissolved oxygen concentration. A is detoxified by deuterium supplied by the solution B. Solution C is a photosynthetic bacterial nutrient, and is preferably a mixture of blood meal and BN bacteria (Meiji Seika). The nutrients supplied by solution C will be the nutrients for the photosynthetic bacteria that are subsequently supplied. Solution D is a mixture of pure cultured photosynthetic bacteria, preferably Chroma
tium buderi, Amoebobacter roseus, Rhodopseudomon
Asacidophila and Rhodopseudomonas capsulata cultured to steady state are preferably 3: 2:
A mixture of photosynthetic bacteria mixed at a ratio of 3: 2 to 2: 1, 1: 1: 2, or 1: 1: 1: 0.5 and suspended in a concentrated active solution at a 2-4 fold dilution. is there. The amount of liquids A to D depends on the degree of contamination of the wastewater in the grease trap.
Usually, adding 250 ml of each liquid is sufficient for pretreatment.

[0026] The starter kit is prepared by introducing the liquid A into the grease strap 5 minutes after supplying the liquid A,
To D solution in order. With the starter kit, the environment inside the green strap is adjusted so that photosynthetic bacteria can grow. Aeration is 10L / 100L / min-40L / 1
00 L / min, preferably 15 L / 100 L / min to 25
Perform at L / 100 L / min.

[0027]

【Example】

(Performance Test) The wastewater in the 300-L capacity grease trap provided in the inn was pretreated with a starter kit, and then the apparatus shown in FIG. 1 was operated for 24 hours to perform the wastewater treatment. The pH (about 25 ° C.), the amount of suspended solids, the biochemical oxygen demand, and the n-hexane extract were measured for the wastewater before treatment with the starter kit and the wastewater after treatment with the device. The results are shown in the table below. The measurement was performed according to JIS K0102.
Performed in accordance with

[0028]

[Table 1]

From the above results, it has been clarified that the method of the present invention can effectively remove and remove fats and oils.

(Safety Test) A mixture of photosynthetic bacteria was cultured on a 1.8 L scale, and the safety of the culture medium after the culture was examined as shown in Table 2 below.

[0031]

The above inspections were performed by the following methods. pH: conforms to the glass electrode method of JIS K 0102 Cadmium concentration: conforms to the ICP emission analysis method of JIS K 0102 Cyanide compound concentration: pyridine- of JIS K 0102
Pyrazolone absorption spectrophotometry Organic phosphorus concentration: Gas chromatographic method according to JIS K 0102 Lead concentration: Based on ICP emission analysis method according to JIS K 0102 Hexavalent chromium concentration: Diphenylcarbazide absorption spectrophotometry according to JIS K 0102 Arsenic concentration: JIS K 0102 Hydride generation atomic absorption spectrometry Total mercury concentration: Reduction vaporization atomic absorption spectrometry according to JIS K 0102 Alkyl mercury concentration: Gas chromatography method according to JIS K 0102 Polychlorinated biphenyl concentration: JIS K 0102

As is clear from the results shown in Table 2, the wastewater treated by the method of the present invention was found to be safe and harmless to the human body.

[0034]

According to the present invention, the treatment of fats and oils in wastewater using a mixture of photosynthetic bacteria having a specific composition is performed. Can be effectively decomposed and removed. Thus, it is not necessary to collect the solidified oil in the grease trap as in the related art, so that labor can be reduced and environmental pollution can be prevented. Also, cleaning inside the grease strap becomes extremely simple.

[Brief description of the drawings]

FIG. 1 is a conceptual diagram of an apparatus for performing the method of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Grease strap 11 Drainage 12 Partition plate 13 Treated water 14 1st tank 15 2nd tank 16 3rd tank 2 Purified liquid supply means 21 Tank 22 Strainer 23 Liquid feed line 24 Pump 25 Purified liquid 26 Discharge port 3 Ventilation means 31 Diafrom Blower 32 Air supply line 33 Air diffuser

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification code FI C12R 1:01)

Claims (3)

[Claims] (1) Chromatium buderi, Amoebobacter roseu
s, Rhodopseudomonas acidophila, and Rhodopseudo
A mixture of photosynthetic bacteria composed of monas capsulata. 2. A method for supplying a mixture of the photosynthetic bacteria according to claim 1 to waste water in a grease trap to decompose and remove oils and fats in the waste water in the grease trap. 3. The pretreatment of wastewater in the grease trap includes a surfactant, a dissolved oxygen supply source, a photosynthetic bacteria nutrient,
3. The method for decomposing and removing oils and fats in wastewater in a grease trap according to claim 2, wherein the mixture of the photosynthetic bacteria according to claim 1 is added.