JPH11313887A - Blood component collection instrument - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an instrument for collecting leucocyte captured by a filter, at high yield. SOLUTION: A blood component collection instrument comprises a filtration part 3 charged with a filter 2 for permeating erythrocyte but capturing leucocyte, and a housing 4 for accommodating a pressing member 1 for pressing the filter 2. A sidewall of the housing 4 is provided with a blood inflow port and a blood outflow port communicating with the filtration part 3, and an upper wall of the housing 4 is provided with a means for moving the pressing member 1 vertically.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood component collection device for collecting leukocytes from a blood mother liquor containing a blood component, and more particularly to a device derived from hematopoietic stem cells and / or hematopoietic progenitor cells from umbilical cord blood, bone marrow and peripheral blood. The present invention relates to a blood component collecting device for collecting white blood cells.

[0002]

2. Description of the Related Art Conventionally, in the treatment of frequent transfusion patients, fever, which is a side effect caused by an alloimmune reaction by leukocytes,
Blood products from which leukocytes have been removed have been used for blood transfusions to prevent non-hemolytic fever reactions such as chills and scars. Such blood products from which leukocytes have been removed have been produced by centrifugation utilizing the difference in specific gravity of blood components or by using a leukocyte removal filter. Among them, the method using a leukocyte removal filter is widely used because the operation is simple and a blood product having a high leukocyte removal rate can be obtained. The leukocytes adsorbed on the filter have been discarded together with the filter.

On the other hand, for hematopoietic disorders caused by chemotherapy for leukemia, cancer, etc., bone marrow transplantation therapy and peripheral blood stem cell transplantation therapy are performed, and hematopoiesis contained in bone marrow and peripheral blood is Hematopoietic disorders have been overcome by transplanting stem cells and hematopoietic progenitor cells into patients. In recent years, it has been found that cord blood contains hematopoietic stem cells and hematopoietic progenitor cells, and development of cord blood stem cell transplantation therapy is also expected. In order to obtain hematopoietic stem cells and hematopoietic progenitor cells to be transplanted into a patient suffering from hematopoietic disorders from a blood mother liquor containing these blood components, cryopreserved cells are thawed and produced (WO96 / No. 17514). If erythrocytes are mixed in the cryopreserved blood, the erythrocytes will lyse at the time of thawing, causing a side effect. Therefore, it is necessary to remove the erythrocytes from the frozen blood in advance. Also, conventionally, removal of platelets from blood has not been a problem, but in recent years removal of cells causing complications in allogeneic transplantation, removal of cancer cells in autologous transplantation,
Since platelets aggregate and adhere to these cells and reduce the cell separation efficiency, it is preferable to also remove platelets from the blood.

[0004] Conventionally, as a method for obtaining leukocytes from whole blood, plasma is brought into contact with a leukocyte separation filter made of a fibrous substance, and leukocytes and other blood components are separated therefrom through a blood cell suspension. A method for collecting leukocytes captured in a separation filter is known (Japanese Patent Publication No. 58-54131). In addition, bone marrow, as a method of collecting leukocytes derived from hematopoietic stem cells and hematopoietic progenitor cells obtained by removing red blood cells and platelets from the blood mother liquor of peripheral blood, a cell population containing red blood cells and hematopoietic stem cells and hematopoietic progenitor cells
JP-A-8-104643 discloses a method in which a liquid is passed through a filter that transmits red blood cells and captures white blood cells, and then induces a liquid flow in a direction opposite to the flowing direction to collect the captured white blood cells. I have. Also, JP-A-9-121849 discloses that
A cell population containing erythrocytes, hematopoietic stem cells, monocytes, granulocytes,
After passing through the red blood cells and passing through the first capturing means for capturing white blood cells, the captured white blood cells are recovered using the recovered liquid,
A method of capturing the collected leukocytes, capturing monocytes and granulocytes, passing the collected leukocytes through a second capturing means passing through the hematopoietic stem cells, and obtaining hematopoietic stem cells flowing out of the capturing means is introduced.

[0005]

However, among the methods of recovering leukocytes captured by these filters, the method of flowing the recovered liquid in the same direction as the first flow direction is the method of recovering leukocytes captured between fibers. In order to wash away the white blood cells, it was necessary to increase the liquid pressure of the recovery liquid, and it was difficult to recover leukocytes at a sufficient recovery rate. Further, the method of inducing a liquid flow in the direction opposite to the first liquid flow direction and collecting the captured leukocytes is different from the method of flowing the liquid flow in the same direction as the first liquid flow direction described above in terms of fiber. Although the gap was disturbed, the recovery rate of leukocytes was increased, but the captured leukocytes could not be sufficiently recovered. An object of the present invention is to provide a device for recovering leukocytes captured by a filter in high yield.

[0006]

That is, the present invention relates to a housing containing a filter filled with a filter for transmitting red blood cells and capturing white blood cells and a pressing member for pressing the filter, wherein the filter is provided on a side wall of the housing. A blood component collection instrument provided with a blood inflow port and a blood outflow port communicating with the section, and further provided with means for moving the pressing member up and down on the upper wall of the housing.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The filter for permeating red blood cells and capturing white blood cells according to the present invention refers to a fibrous or porous aggregate, for example, regeneration of synthetic fibers such as polyester, polyamide, polyacrylonitrile, cellulose, acetate and the like. It is an aggregate of fibers, inorganic fibers such as glass, natural fibers such as cotton, porous materials such as foamed molded products, sintered bodies, and spherical materials such as hydroxyapatite beads. When platelets are allowed to pass through the filter together with red blood cells, a polymerizable polymer having a nonionic hydrophilic group such as hydroxyethyl methacrylate on the surface of the material constituting the aggregate as described in JP-B-6-51060. Coating a copolymer of a compound and a polymerizable compound having a basic nitrogen-containing functional group such as diethylaminoethyl methacrylate, or a polymerizable compound having a hydroxyl group as described in JP-A-1-249063. It is obtained by forming fibers of a modified polymer obtained by copolymerizing or graft-polymerizing a polymerizable compound having a methacrylic acid group.

When the filter is made of a fiber aggregate, the filter is made of fibers having a fiber diameter of 25 microns or less, preferably 10 microns or less, and more preferably 0.5 to 3 microns. If the fiber diameter exceeds 25 microns, the yield of leukocytes captured by the filter tends to decrease. Further, the bulk density of the fiber aggregate is 0.05 to 0.50 g / cm ³ , preferably 0.08 to 0.30 g / cm ³ , more preferably 0.10 to 0.20 g / cm ³
It is. When the bulk density is less than 0.05 g / cm ^3, tend to yield leukocytes trapped in the filter is low, the bulk density exceeds 0.50 g / cm ^3, slow the flow rate of blood through the filter Tend to be. As a fiber aggregate, 1
The layer may be, for example, a laminate of two or more nonwoven fabrics formed from fibers having a fiber diameter of 25 microns or less.

When the filter is composed of a multi-layered fiber assembly, at least one layer is formed of fibers having a fiber diameter of 25 μm or less and has a bulk density of 0.05 to 0.50 g / cm ^3. . The multilayer structure has two to six layers. A fiber aggregate having a large fiber diameter and bulk density is arranged in a layer near the blood inlet, and a fiber aggregate having a small fiber diameter and bulk density is arranged in a layer near the blood outlet. This is preferred because the leukocytes are trapped in order from the largest diameter. For example, the filter fiber assembly of the first layer bulk density formed from textiles fiber diameter 10 microns is 0.23 g / cm ^3, a bulk density of the second layer is formed from a fiber diameter of 3.5 micron fibers
In the case of a multi-layered fiber assembly consisting of a fiber aggregate of 0.11 g / cm ³ and a bulk density of 0.12 g / cm ³ formed of fibers having a fiber diameter of 1.5 microns, Is a substance having a large diameter in blood, monocytes and granulocytes are captured in the second layer, and lymphocytes are captured in the third layer. Examples of the multi-layered fiber assembly include, for example, 12 non-woven fabrics in which the first layer is made of fiber having a fiber diameter of 10 microns, three non-woven fabrics in which the second layer is made of fiber having a fiber diameter of 3.5 micron, and the third layer is made of a fiber diameter. The bulk density of two non-woven fabrics consisting of 1.8 micron fibers is 0.11 g /
fiber assembly is cm ^3, and or bulk density first layer two nonwoven and second layer of fibers 10 micron diameter fibers are a nonwoven fabric 14 sheets of fiber diameter 3.5 microns fiber 0.13 g / cm ³ and the like. When the filter material is made of a porous material, the pore size is preferably 1 to 500 microns.

[0010] The blood mother liquor referred to in the present invention refers to human blood, animal blood, bone marrow, peripheral blood, umbilical cord blood, etc., and the blood components are derived from monocytes, granulocytes, lymphocytes, hematopoietic stem cells, hematopoietic progenitor cells. White blood cells, red blood cells, platelets and the like. As a cleaning solution,
Physiological saline, Hank's solution (HBSS), Dulbeccoline (D-PB
Buffer solutions such as S) and those obtained by adding a protein such as phytoserum albumin or an anticoagulant to these buffer solutions. The present invention allows the blood mother liquor containing these blood components to pass through a filtration unit inside a housing filled with a filter that transmits red blood cells and captures white blood cells, and further increases the internal volume of the filtration unit. This is a blood component collection instrument for collecting white blood cells by passing a washing solution. The filter may be directly filled in the filtering portion inside the rigid housing, or may be filled in a bag made of a flexible resin having a blood inflow tube and a blood outflow tube, and the bag may be filled in the filtering portion inside the rigid housing. It may be configured to be accommodated in the storage device. The filter made of the flexible resin is compressed and filled with the filter, and the inner volume of the bag increases as the inner volume of the filtering section increases.

Examples of the housing material include synthetic resins such as polycarbonate, polystyrene, polyolefin, and rigid polyvinyl chloride, and metals such as stainless steel and aluminum. The bag is formed by welding the edges of two sheets made of a flexible resin, and having a blood inlet,
A rigid port at the blood outlet is attached. Examples of the flexible resin constituting the bag include polyester, polyolefin, polyurethane, ethylene-vinyl acetate copolymer, and soft polyvinyl chloride.

The present invention is characterized in that after the blood mother liquor has passed through the inside of the filter, the internal volume of the filtration section inside the housing is further increased, and the washing solution is passed therethrough to collect the white blood cells captured by the filter. And The size at which the filtration section inside the housing expands is such that the internal volume of the filtration section after the blood mother liquor passes through the filter is at least as large as the internal volume of the filtration section before the blood mother liquor passes through the filter. 1.10 times, preferably 1.20 to 1.80 times, more preferably 1.
It is 30 to 1.60 times. If the magnification for enlarging the filtration section is less than 1.10, the white blood cells captured by the filter tend not to be sufficiently collected, and if the magnification is too large, the housing tends to be large.

[0013] The blood component collection device of the present invention includes a filtration unit filled with a filter that transmits red blood cells and captures white blood cells;
A pressure member that presses the filter is housed therein; a blood inlet and a blood outlet that communicate with the filtration unit are provided on the housing side wall; Is provided. The pressing means is moved downward by the moving means, and after the blood mother liquor containing the blood component is passed through the filtration unit, the pressing member is moved upward to increase the volume of the filtration unit. Then, leukocytes are collected by passing the washing solution.

FIG. 1 shows an embodiment of the blood component collecting instrument of the present invention.
The description will be made using the blood component sampling instrument of the present invention. FIG. 1 shows a state in which the upper lid part (14) and the lower lid part (15) are completely screwed together to form a housing (4), and the filter (2) is pressed to a stage before the blood mother liquor flows. FIG. 2 is a schematic view of a blood component collecting instrument, in which a lower lid (15) has a filtering part (3) containing a filter (2), and a side wall of the blood inlet (10) and a blood outlet (11); Is provided, and a pressing member (1) is placed on the filter (2). The pressing member (1) has a bar-shaped member (5) extending vertically upward, and the tip of the bar-shaped member (5) has a head (7) having a larger diameter than the bar-shaped member (5); A male screw is formed on the peripheral wall of the head (7). The upper lid (14) has a recess (6) formed in the upper wall of the housing (4), and has a hole (19) formed at the bottom thereof so that the bar-shaped member (5) can move up and down.

In this blood component collecting instrument, in order to move the pressing member (1) up and down, a cylindrical body having an outer diameter which can be inserted into the recess (6), and a head (7) on the inner wall of the cylindrical body. This is performed using a nut portion (8) having a handle portion (27) formed with a female screw (9) that can be screwed with the male screw.
The pressing member (1) is in the shape of a disk, and the periphery thereof is O
A ring (18) is provided to slide the inner wall of the lower lid (15) in a liquid-tight manner. Female screw (17) is formed on the inner wall of the upper lid (14) opening, and male screw (16) is formed on the outer wall of the lower lid opening.
And the male screw (16) are screwed together to form the housing (4). In FIG. 1, the housing (4) is formed by screwing, but may be formed by fitting and bonding. In FIG. 1, the rod-shaped member (5) has a head (7) at the upper end, and has a structure in which a male screw is formed on the peripheral wall of the head (7).
The external thread may be formed on the peripheral wall of the rod-shaped member (5). In FIG. 1, the female screw (9) of the nut (8)
A locking portion may be provided in the nut portion (8) so that the screw engagement with the male screw of the head (7) of the rod-shaped member (5) is not loosened when fixed.

In order to collect leukocytes from a blood mother liquor using the blood component collecting instrument shown in FIG. 1, a nut (8) is inserted into the recess (6), and a female screw (9) of the nut (8) is inserted. Is screwed into a male screw of the head (7) of the rod-like member (5), and the nut-like part (8) is rotated, whereby the rod-like member (5) moves downward, and the pressing member (1) Press (2). After the filtration unit (3) has a predetermined volume, the blood mother liquor is passed from the blood inflow tube (12) to the filter (2) through the blood inlet (10) to capture leukocytes in the filter (2). . Then, rotate the nut (8) in the reverse direction,
After moving the rod-shaped member (5) upward to increase the volume of the filtration part (3), the washing liquid is passed through the filter (2), and the white blood cells captured by the filter (2) are removed together with the washing liquid. The blood flows out from the blood outflow tube (13) through the blood outlet (10) and is collected.

FIG. 2 is a schematic view showing another embodiment of the blood component collecting device of the present invention, in which the filter (32) is pressed to a stage before the blood mother liquor is passed, and the state of the blood component collecting device is shown. Is shown. The filter (32) is housed in a bag (31) made of a flexible resin, and the bag (31) connects the blood inflow tube (42) and the blood outflow tube (43). The blood inflow tube (42) and the blood outflow tube (43) are connected to the outside through a blood inlet (44) and a blood outlet (45) formed on the side wall of the housing (30). A pressing member (33) is placed above the filter (32). Above the pressing member (33), a locking portion (35) is accommodated, and a chamber (34) in which a first hole (41) through which a rod-shaped member (36) penetrates is formed is provided at an upper portion. The locking portion (35) is formed at the bottom of the rod-shaped member (36), has a larger diameter than the rod-shaped member (36), and can rotate freely in the chamber (34). Male screw (39) on the periphery of the rod-shaped member (36)
Is formed, and is screwed with a female screw of a second hole (40) formed in the upper wall of the housing (30) to move the rod-shaped member (36) up and down. A head (37) having a diameter larger than that of the rod-shaped member (36) is provided at the upper end of the rod-shaped member (36), and is connected to a handle (38) for rotating the rod-shaped member (36). In FIG. 2, since the filter (32) is accommodated in the bag (31) at the periphery of the pressing member (33), the O-ring (18) is not necessarily the same as the pressing member (1) in FIG. Not necessary.

In order to collect white blood cells from the blood mother liquor using the blood component collection device shown in FIG. 2, by rotating the handle (38), the rod-like member (36) moves downward, and the pressing member (33) moves. ) Presses the filter (32). After the bag (31) has a predetermined volume, the blood mother liquor is transferred to the blood inflow tube (42).
Through the bag (31) to capture leukocytes in the filter (32). Thereafter, the handle (38) is rotated in the reverse direction to move the rod-shaped member (39) upward to increase the volume of the bag (31), and then the washing liquid is passed through the bag (31), and the filter (32)
The leukocytes trapped in the blood flow through the blood outlet (45) and the blood outflow tube (43) to the outside together with the washing liquid, and are collected.

FIG. 3 is an explanatory diagram of a method for collecting blood components using the blood component collection device of FIG. A filter (2) in which whole blood is pressed from the blood bag (21) containing whole blood through the three-way cock (25), through the blood inflow tube (12), and to the predetermined volume in FIG. Flows into the filtration unit (3) containing the. White blood cells are trapped in the fiber gap, for example, inside the filter (2), while red blood cells pass through the fiber assembly and pass through the blood outlet (11) to the blood outflow tube.
(13) It is stored in the blood bag (23) via the three-way cock (26). The blood child bag (23) can contain platelets in addition to red blood cells, depending on the filter material.

Next, the handle (27) shown in FIG. 1 is rotated in the reverse direction to move the rod-shaped member (5) upward, thereby increasing the volume of the filter (3). Thereafter, the washing liquid flows from the washing liquid bag (22) into the blood component collecting instrument (20) through the three-way cock (25). At this time, the washing liquid is temporarily stopped from flowing into the leukocyte storage bag (24) with the three-way cock (26), the washing liquid is filled in the filter (2), the fiber gap is widened, and then the three-way cock (26) Is preferably opened in the direction in which it flows into the leukocyte storage bag (24). The leukocytes trapped in the fiber gap inside the filter (2) of the blood component collecting instrument (20) are washed with the washing liquid by the increase in the fiber gap and the flow pressure of the washing liquid, and pass through the three-way cock (26). Are stored in a white blood cell storage bag (24).

[0021]

EXAMPLES Examples of the present invention will be specifically described below with reference to examples. Example 1 In the blood component collection device of FIG.
Is composed of a disc-shaped three-layer nonwoven fabric (diameter: 4.86 cm) formed from polyethylene terephthalate fiber. When the filter (2) is pressed by the pressing member (1) to a predetermined volume, the structure of the three-layer nonwoven fabric is such that the first layer of the upper layer has a bulk density of 0.23 g / cm formed of fibers having a fiber diameter of 10 microns.
³ , the second layer of the intermediate layer is a non-woven fabric having a bulk density of 0.11 g / cm ³ in which the second layer is formed from fibers having a fiber diameter of 3.5 microns, and the third layer of the lower layer is formed of fibers having a fiber diameter of 1.5 microns. Consists of a nonwoven fabric of 0.12 g / cm ³ . Its volume ratio is 30:
At 22:48, the overall thickness was 8.1 mm. 100 ml of bovine blood containing an ACD solution as an anticoagulant is allowed to flow at a flow rate of 5 ml / min to capture white blood cells inside the filter (2), and red blood cells pass through the filter (2) and pass through the blood bag (23). Was housed in The recovery rate of red blood cells recovered in the blood child bag (23) was 95%, and the recovery rate of platelets was 19%. Then
After closing the three-way cock (26), the handle (27) in FIG. 1 was reversely rotated to move the pressing member (1) upward. After that, the filter (2) is filled with physiological saline to widen the fiber gap, and then 150 ml of physiological saline is flowed through the filtration part (3) at a flow rate of 5 ml / min, and stored in the leukocyte storage bag (24). did. Table 1 shows the expansion rate of the volume of the filtration unit (3) filled with the filter (2) due to the upward movement of the pressing member (1) and the recovery rate of the white blood cells stored in the white blood cell storage bag (24). Show.

The volume expansion rate is determined by filtering whole blood (2).
Loosen the screw to the thickness (distance from the bottom of the lower lid to the pressing member) of the filter (2) before passing the liquid through,
This is the ratio of the distance from the bottom of the lower lid to the pressing member when the pressing member is moved upward. The leukocyte recovery rate is a ratio of the number of leukocytes in the physiological saline contained in the leukocyte containing bag (24) to the number of leukocytes in the blood contained in the blood bag (21).

[0023]

[Table 1]

As is evident from Table 1, as the volume expansion rate increases, the leukocyte recovery rate increases.

Example 2 A non-woven fabric formed from fibers having a fiber diameter of 10 microns and a non-woven fabric formed from fibers having a fiber diameter of 1.5 microns
After immersion in a 0.25% ethanol solution of a copolymer of hydroxyethyl methacrylate and diethylaminoethyl methacrylate, the first layer is made of a non-woven fabric having a fiber diameter of 10 microns.
A filter was obtained in which the second layer was a nonwoven fabric having a fiber diameter of 1.5 microns. The first layer is the upper layer and the second layer is the lower layer (volume ratio 60:40) to form a disk-shaped filter (diameter 3.82 cm, thickness 8.6 mm), which is collected as shown in FIG. The instrument was housed in a low-density polyethylene bag (31). The handle (38) of the blood component collecting instrument of FIG. 2 was rotated to move the rod-shaped member (36) downward to press the filter (32). When the bag (31) is pressed to a predetermined volume by the pressing member (33), the structure of the two-layer nonwoven fabric is as follows.
10 microns, a bulk density of 0.23 g / cm ³ nonwoven, second layer fiber diameter 1.5 microns, a bulk density is a nonwoven fabric of 0.12 g / cm ^3.

50 ml of umbilical cord blood containing a heparin solution as an anticoagulant is allowed to flow at a flow rate of 5 ml / min to capture leukocytes inside the filter.
And was stored in the blood bag (23). The recovery rate of red blood cells recovered in the blood child bag (23) was 89%, and the recovery rate of platelets was 72%. Next, after closing the three-way cock (26), the handle (38) in FIG.
3) was moved upward. Then, pour the saline (3
1) Fill the inside and widen the fiber gap, then flow 120 ml of physiological saline at a flow rate of 5 ml / min into the bag (31) containing the filter (32), and store leukocytes from the blood outflow tube (43). Housed in bag (24). Table 2 shows the expansion rate of the volume of the bag (31) due to the upward movement of the pressing member (33) and the recovery rate of the white blood cells stored in the white blood cell storage bag (24).

[0027]

[Table 2]

As is evident from Table 2, as the volume expansion rate increases, the leukocyte recovery rate increases.

[0029]Example 3 In the blood component collection device shown in FIG.
Fibrous aggregate of disk-shaped two-layer structure of terephthalate fiber
(5.6 cm diameter) was used. 2
The layer is 0.82 thick formed of fibers having a fiber diameter of 10 μm.
2 mm nonwoven fabric (upper layer) and 3.5 μm fiber
30 non-woven fabrics of 0.38 mm thickness formed from fibers (bottom
Layers). When the filter is pressed by a pressing member,
Bulk density is 0.19g / cm^ThreeMet. AC as anticoagulant
120 ml of bovine blood containing solution D was flowed at a flow rate of 5 ml / min.
Through the filter to capture leukocytes inside the filter,
Red blood cells were allowed to pass. Then, add 40 ml of physiological saline
After passing through the filter, the red blood cells and remaining
And platelets flowed out. Next, the internal volume of the filter is reduced.
Bulk density 0.11g / cm ^ThreeAfter expanding to bovine serum albumin
80 ml of physiological saline containing
Collected. Change the internal volume of the filter for comparison.
Instead, the physiological saline was added to the filter in the same manner as in the above method.
Leukocytes were collected by washing in a luter. Table of leukocyte yield
3 is shown.

[0030]

[Table 3]

As is clear from Table 3, the leukocyte recovery rate was improved by reducing the bulk density of the filter at the time of recovery.

Example 4 A filter was used in the same manner as in Example 3 except for the amount of bovine blood.
Leukocytes were collected by passing 50 ml of bovine blood containing the CD solution. Table 4 shows the leukocyte recovery rate.

[0033]

[Table 4]

As is clear from Table 4, the leukocyte recovery rate was improved by reducing the bulk density of the filter at the time of recovery.

[0035]

The blood component collecting device of the present invention is a simple and compact device, and can collect white blood cells captured by the filter in high yield.

[Brief description of the drawings]

FIG. 1 is a schematic view of a blood component collecting instrument showing one embodiment of the present invention.

FIG. 2 is a schematic view of a blood component collecting instrument according to another embodiment of the present invention.

FIG. 3 is an explanatory diagram of collecting white blood cells using the device of the present invention.

[Explanation of symbols]

 1, 33 pressing member 2, 32 filter 3 filtration part 4, 30 housing 5, 36 rod-shaped member 6 recessed part 7 head 8 nut part 12, 42 blood inflow tube 13, 43 blood outflow tube 20 blood component collection instrument 21 blood bag 22 Cleaning solution bag 23 Blood child bag 24 White blood cell storage bag 27, 38 Handle 31 bags

Claims (8)

[Claims] 1. A housing containing a filter section filled with a filter that transmits red blood cells and traps white blood cells, and a pressing member that presses the filter, wherein a blood inlet and blood are provided on a side wall of the housing and communicate with the filtering section. A blood component collection instrument provided with an outlet, and further provided with means capable of moving the pressing member up and down on the upper wall of the housing. 2. The pressing member moves downward while the blood mother liquor containing the blood component passes through the filtering portion, and then the pressing member moves upward to increase the volume of the filtering portion. The blood component collection device according to claim 1, wherein the leukocytes are collected by passing through a washing solution. 3. A means for vertically moving the pressing member includes a bolt portion formed of a rod-shaped member having a male thread formed on a peripheral wall extending vertically above the pressing member, and a concave portion formed on an upper wall of the housing. And a cylindrical body having an outer diameter capable of being inserted into the recess, wherein the inner wall of the cylindrical body can be screwed with a male screw of the bolt part. 3. The blood component collection device according to claim 1, comprising a nut portion having an internal thread. 4. A means for moving the pressing member up and down includes a rod-shaped member extending vertically above the pressing member and having a bottom portion formed with a male screw on a peripheral wall formed of a locking portion, and containing the locking portion. A pressing member provided with a chamber in which a first hole through which the bar-shaped member penetrates is formed, and a second hole formed in an upper wall of the housing, and an inner wall of the second hole is provided with a male screw of the bar-shaped member. 3. The blood component collection device according to claim 1, wherein a female screw that can be screwed is formed. 5. The blood component collection device according to claim 1, wherein the filter is housed in a bag made of a flexible resin, and the bag connects a blood inflow tube and a blood outflow tube. 6. The blood component collecting instrument according to claim 1, wherein the pressing member has a disk shape, and a peripheral wall of the pressing member is provided with an O-ring capable of sliding on the inner wall of the housing in a liquid-tight manner. . 7. The filter is a fiber having a fiber diameter of 25 microns or less and a bulk density of 0.05 to 0.50 g / cm ^3.
The blood component collecting instrument according to any one of claims 1 to 6, which comprises a fiber assembly of (1). 8. The filter as claimed in claim 1, wherein the filter comprises a multilayer fiber assembly.
And at least one layer has a fiber diameter of 25 microns or less.
And a bulk density of 0.05 to 0.50 g / cm ³Fiber
The blood component according to any one of claims 1 to 7, which is an aggregate.
Collection equipment.