JP3230232B2 - Filter set - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a filter set for recovering mainly leukocytes from blood.

[0002]

2. Description of the Related Art It has been known that chemotherapy for hematopoietic tumors such as leukemia and solid tumors causes side effects such as hematopoietic disorders. In order to eliminate such side effects, bone marrow transplantation treatment for transplanting hematopoietic stem cells and / or hematopoietic progenitor cells contained in bone marrow and peripheral blood, peripheral blood stem cell transplantation treatment, and the like have been performed. More recently,
It has been found that cord blood also contains hematopoietic stem cells and / or hematopoietic progenitor cells, and a therapeutic method of transplanting the hematopoietic stem cells and / or hematopoietic progenitor cells is also expected. Usually, blood used for these transplantation treatments is cryopreserved from the time of collection until the time of transplantation. Here, if erythrocytes are mixed in the blood to be cryopreserved, the erythrocytes will cause hemolysis after thawing, thereby causing side effects. Therefore, it is necessary to remove the erythrocytes in the transplanted blood before freezing. As a method for removing red blood cells from transplanted blood, conventionally, a centrifugal separation method utilizing the difference in specific gravity between red blood cells and leukocytes derived from hematopoietic stem cells and / or hematopoietic progenitor cells, or leukocytes by allowing blood to pass therethrough using a leukocyte removal filter A method is known in which leukocytes derived from hematopoietic stem cells and / or hematopoietic progenitor cells captured by a removal filter are passed through a washing solution to be collected.

[0003]

However, the centrifugal separation method requires skill so as not to disturb the interface between separated blood components, and the recovery method using a filter requires a method of recovering hematopoietic stem cells and / or hematopoietic progenitor cells. Since the packing density of the filter material is increased in order to capture leukocytes at a high concentration, there is a drawback that the leukocytes adhered to the filter material are hardly peeled off when the washing is performed with a washing solution, and the recovery rate is poor. The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a filter set for efficiently collecting leukocytes mainly from blood.

[0004]

Means for Solving the Problems The inventors of the present invention have conducted intensive studies to achieve the above object, and as a result, have stored a bag body filled with a filter material therein, and compressed the bag body in a thickness direction. The present inventors have found that by combining a flexible tube with a flexible tube, it is possible to efficiently recover mainly leukocytes from blood and arrived at the present invention. That is, the present invention
A bag body having a blood inlet and a blood outlet and filled with a filter material therein, and a flexible tube body that stores the bag body in a compressed state in a thickness direction, wherein the bag body is the tube. It is a filter set characterized by being removable from the body. As the tube body, a heat-shrinkable tube, a tube having the same length as the bag body, and having a smaller volume than the bag body can be used. Here, when the tube body is a heat-shrinkable tube, it is preferable that a break portion is provided, and when the tube body has the same length as the bag body and has a smaller volume than the bag body, Preferably, at least one end of the tube body is provided with a grip for detaching the tube body from the bag body.

[0005]

Embodiments of the present invention will be described with reference to the drawings. FIG. 1 is a perspective view showing one embodiment of the filter set of the present invention, FIG. 2 is a longitudinal sectional view of the filter set shown in FIG. 1, and FIG. 3 is a perspective view showing another embodiment of the filter set of the present invention. It is.

As shown in FIGS. 1, 2 and 3, the filter set comprises a bag 1 filled with a filter material 11, a tube 21 connected to a blood inlet 12 of the bag 1, and It comprises a tube 22 connected to the blood outlet 13 and a flexible tube 3 or 4 for accommodating the bag 1 in a compressed state in the thickness direction.

The bag 1 is made of a flexible sheet, and its edge is welded and formed by a method such as heat welding, high frequency welding, ultrasonic welding, or solvent welding. As the material of the bag 1, synthetic resins such as soft polyvinyl chloride, ethylene-vinyl acetate copolymer, styrene-butadiene-styrene copolymer, polyurethane, polyamide, polyester, polyethylene and polypropylene are preferably employed.

The bag 1 is filled with a filter material 11 as shown in FIG. The filter material 11 is
It is mainly for capturing leukocytes from blood, and synthetic fibers made of polyester, polypropylene, polyethylene, polymethyl methacrylate, polyamide and the like, and natural fibers such as cotton are preferably used. Filter material 1
The fiber diameter of 1 is preferably in the range of 0.1 to 40 μm, and if it is less than 0.1 μm, the fiber spacing per unit area tends to be small and the filtration resistance tends to be large.
If it exceeds m, the fiber volume tends to increase, and the amount of excess blood component adsorbed tends to increase. In addition, filter material 11
The filling amount of may be an amount which can obtain a capture rate of a degree which a normal leukocyte removal filter has in a compressed state.

The blood inlet 12 and the blood outlet 13 are arranged on opposite sides with respect to the filter material 11, and blood flowing from the blood inlet 12 passes through the filter material 11 and flows out of the blood outlet 13. It has become.
Similarly, the washing liquid flowing from the blood inlet 12 or the blood outlet 13 passes through the filter material 11 and flows out from the blood outlet 13 or the blood inlet 12. The washing solution is for washing out and collecting blood components attached to the filter material 11, and is a saline solution, Hanks' solution, Dulbecco's salt buffer, or the like, or a solution obtained by adding human serum albumin or an anticoagulant thereto. Are preferred.

A tube 21 is connected to the blood inlet 12, and a tube 22 is connected to the blood outlet 13. Examples of the connection method include welding, bonding, and connection using a connector. In the case of connection by a connector, the bag 1 and the tubes 21 and 22 are aseptically connected at the time of use. Incidentally, the filter set of the present invention is usually adopted in a configuration in which one ends of tubes 21 and 22 are connected to the bag body 1 and a blood bag (not shown) is connected to the other ends of the tubes 21 and 22. But the tube 21,
It is also possible to use a syringe or the like that can be connected to the blood inlet 12 and the blood outlet 13 without connecting the 22.

The tube body 3 shown in FIG. 1 is a heat-shrinkable tube molded in a single-layer or multi-layer structure with a synthetic resin such as polyvinyl chloride, polyester, polypropylene, polyethylene, and polystyrene. The bag 1 is contracted by heating and presses the bag 1 in a desired contracted state. At one end (the tube 22 side) of the tube body 3, for example, a V-shaped cut 31 may be provided as a break, and a perforation 32 may be provided from the cut 31 in the longitudinal direction. After the blood is passed through the bag 1, the tube 3 can be easily broken along the perforations 32. Although not particularly limited, the tube body 3 is usually formed to a thickness of about 10 to 100 μm.

As shown in FIG. 3, a tube body having a length equal to that of the bag body 1 and having a smaller volume than the bag body 1 is adopted as shown in FIG. It can also be done. The tube body 4 may be provided at one end (on the tube 22 side) with a grip portion 41 for removing the tube body 4 from the bag body 1. The tube body 4 can be removed by sliding after the blood is passed through the bag body 1. When the bag body 1 is supported by one hand and the grip portion 41 is pulled by the other hand, the tube body 4 is pulled. ,
It can be easily slid and removed from the bag 1. In addition, as a material of the tube body 4, a synthetic resin such as polyvinyl chloride, polyethylene, or polypropylene is preferably employed. The grip portion 41 may be formed of a synthetic resin such as polypropylene or polyethylene. However, when the grip portion 41 is provided in a rib shape along the edge of the tube body 4 as in this example, the same as the tube body 4 is used. It is preferable to be formed integrally with the material. Note that the tube body is not limited to the shapes shown in FIGS. 1 and 3 as long as the bag body 1 can be compressed so as to obtain a capture rate of a level which a normal leukocyte removal filter has.

Next, a method of recovering leukocytes derived from hematopoietic stem cells and / or hematopoietic progenitor cells from cord blood will be described with reference to FIG. First, umbilical cord blood is passed from the blood inlet 12 to the blood outlet 13 with the bag 1 accommodated in the tube 3. At this time, the filter material 11 is compressed so as to have an appropriate gap, so that leukocytes derived from hematopoietic stem cells and / or hematopoietic progenitor cells can be reliably captured. Next, the tube body 3 is separated from the broken portion 31 by a perforation 32.
The bag 1 is taken out of the tube body 3 and the washing solution is supplied from the blood outlet 13 to the blood inlet 1
Pass through to 2. At this time, since the compression of the filter material 11 is released and the gap is increased, the leukocytes derived from the hematopoietic stem cells and / or hematopoietic progenitor cells adhered to the filter material 11 are easily peeled off, and are easily washed off and recovered with the washing liquid. be able to. In addition, since the gap between the filter materials 11 is large, the cleaning liquid easily passes and the time required for the passage of the cleaning liquid can be reduced. Here, the cleaning liquid may flow from the blood inlet 12 or from the blood outlet 13. Further, the washing solution containing leukocytes derived from hematopoietic stem cells and / or hematopoietic progenitor cells is once collected in a blood container, and then separated by a method such as centrifugation or passing through a filter again.
Alternatively, hematopoietic progenitor cells are collected. The filter used here captures granulocytes and monocytes, but hematopoietic stem cells and / or
Alternatively, those that allow hematopoietic progenitor cells to pass through are preferably employed.

[0014]

As is apparent from the above description, the filter set of the present invention enables efficient collection of blood components attached to the filter material.
Further, the time required for passing the cleaning liquid can be reduced.

[Brief description of the drawings]

FIG. 1 is a perspective view showing one embodiment of a filter set of the present invention.

FIG. 2 is a longitudinal sectional view of the filter set shown in FIG.

FIG. 3 is a perspective view showing another embodiment of the filter set of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Bag body 11 Filter material 12 Blood inflow port 13 Blood outflow port 21, 22 Tube 3, 4 Tube body 31 Cut 32 Perforation 41 Holding part

──────────────────────────────────────────────────続 き Continued on the front page (58) Fields surveyed (Int. Cl. ⁷ , DB name) A61M 1 / 02,1 / 34 A61B 5/15 A61K 35/14 G01N 33/48

Claims (5)

(57) [Claims] 1. A bag having a blood inlet and a blood outlet and filled with a filter material therein, and a flexible tube for accommodating the bag in a compressed state from a thickness direction. A filter set, wherein a bag is removable from the tube. 2. The filter set according to claim 1, wherein the tube is a heat-shrinkable tube. 3. The filter set according to claim 2, wherein a break portion is provided in the tube body. 4. The tube body has a length equal to the bag body,
The filter set according to claim 1, wherein the filter set has a smaller volume than the bag body. 5. The filter set according to claim 4, wherein at least one end of the tube body is provided with a grip for removing the tube body from the bag body.