JPH11290059A - Incubator filled with carbon dioxide - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an antimicrobial carbon dioxide incubator carrying an antimicrobial agent on an inner tank and equipped with a UV light lamp. SOLUTION: This carbon dioxide incubator carries an antimicrobial agent on an inner tank and is equipped with a UV light lamp. Therein, the antimicrobial agent is carried by either of kneading, coating, printing, host stamping, adhering and plating methods. The antimicrobial agent to be carried is selected from the group consisting of organic antimicrobial agents, antimicrobial agents (haloaryl sulfone-based antimicrobial agents, iodopropargyl- based antimicrobial agents, N-haloalkylthio-based antimicrobial agents, benzothoazole-based antimicrobial agents, pyridine-based antimicrobial agents, 8-oxyquinoline-based antimicrobial agents, benzothiazole-based antimicrobial agents, triazine and thiadiazine-based antimicrobial agents, anilide-based antimicrobial agents, adamantine-based antimicrobial agents, dithiocarbamate- based antimicrobial agents, inorganic salt-based antimicrobial agents, brominated indanone-based antimicrobial agents, benzyl bromacetate or the like) used in coatings, and inorganic antimicrobial agents.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a carbon dioxide gas incubator characterized in that an antibacterial agent is carried in an inner tank, and a carbon dioxide gas incubator characterized by installing an ultraviolet lamp and an ultraviolet lamp. The present invention relates to a light-shielding culture container for use in a carbon dioxide gas incubator, and a method for culturing cells using them.

[0002]

2. Description of the Related Art At present, an inner tank of a carbon dioxide incubator used for culturing cells is not subjected to antibacterial treatment and antifungal treatment. Also, no ultraviolet lamp is installed. The device is used under conditions of high temperature and high humidity of 37 ° C., and is designed so that carbon dioxide gas flows into the inside of the culture vessel.
Therefore, if a long time elapses after purchase, a large number of bacteria and fungi that prefer high temperature and high humidity will proliferate in the inner tank, and as a result, bacterial and fungal contamination will frequently occur in flasks and petri dishes used for cell culture, Significant economic losses.

[0003]

SUMMARY OF THE INVENTION An object of the present invention is to solve the problems of bacterial contamination and fungal contamination which frequently occur in the conventional carbon dioxide gas incubator. That is, a carbon dioxide incubator characterized by carrying an antibacterial and antifungal agent in the inner tank, a carbon dioxide incubator characterized by installing an ultraviolet lamp, a light-shielded culture vessel, and cultivation of cells using them It is an object to provide a method.

[Means for Solving the Problems]

[0004] The structure of the present invention for achieving such an object comprises the following technical means (1) to (9). (1) A carbon dioxide incubator characterized in that an antibacterial and antifungal agent is carried on the entire inner tank (ceiling, shelf, shelf support, side surface, internal door, bottom, container for water). (2) The loading of the antibacterial and antifungal agent is performed by kneading, painting, printing,
The carbon dioxide incubator according to (1), which is formed by any one of hot-stapping, sticking, and plating. (3) The antibacterial agent to be supported is an organic antibacterial antifungal agent (formaldehyde condensing bactericide, thiazolone, quaternary ammonium salt, phosphonium salt, pyridinium salt, halogen group bonded to carbon to which electron-withdrawing group is bonded). Compound, aromatic compound having halogen group activated by electron withdrawing group, compound having carbon-carbon double bond or triple bond activated by halogen atom, trihalomethylthio compound, thiocyanato compound, benzimidazole derivative , Phenols, organic arsenic antibacterials, organic tin antibacterials, organic copper antibacterials, organic iodine antibacterials, isothiazoline antibacterials, pyrithione antibacterials, nitrile antibacterials, triazine antibacterials, haloalkylthios Antibacterial agents, paint antibacterial agents (haloallyl sulfone antibacterial agents, iodopropargyl) Antimicrobials, N- haloalkylthio antimicrobial agent, Benz thiazole antibacterial agent, Nitochiriru antibacterial agent, pyridine-based antibacterial agent, 8-oxyquinoline-based antimicrobial agent,
Benzothiazole antibacterial, triazine and thiadiazine antibacterial, anilide antibacterial, adamantane antibacterial, dithiocarbide antibacterial, inorganic salt antibacterial, brominated indanone antibacterial, benzyl bromacetate), inorganic antibacterial The carbon dioxide incubator according to (1), which is selected from the group consisting of agents. (4) The loading of the antibacterial and antifungal agent is based on additives (plasticizer, antioxidant, stabilizer, ultraviolet absorber, antistatic agent, flame retardant, colorant, pigment, foaming agent, active agent, release agent, (1) The carbon dioxide incubator according to (1), which is carried out together with at least one of a polymerization initiator, an emulsifier, an emulsion stabilizer, and a filler. (5) The carbon dioxide incubator according to (1), wherein an ultraviolet lamp is provided in the inner tank. (6) A light-shielding culture container for use in the carbon dioxide incubator according to (5). (7) A method for culturing cells using the carbon dioxide incubator according to (1) or (5). (8) A method for culturing cells using the light-shielding culture container according to (6). (9) The carbon dioxide incubator according to (1) or (5), wherein a disinfectant filter is provided at an inlet of the carbon dioxide in the inner tank.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION The carbon dioxide gas incubator of the present invention is characterized in that an antibacterial agent is carried on the entire inner tank. A method in which a coating containing an antibacterial agent containing an ultraviolet-curable resin as a main component is applied as a top coat on the tank surface, and the coating is cured by irradiating ultraviolet rays. (2) A method in which a paint containing an antibacterial agent dissolved in an organic solvent is applied to the inner tank surface of a carbon dioxide gas incubator, and the coating film is dried and cured. (3) A method of attaching a plate containing an antibacterial agent to the inner tank surface of a carbon dioxide incubator. (4) A method of kneading an antibacterial agent into metal and plastic constituting an inner tank of a carbon dioxide gas incubator. (5) A method of printing an antibacterial agent on the inner tank surface of a carbon dioxide incubator. (6) A method of carrying an antibacterial agent on the inner tank surface of a carbon dioxide incubator by hot-stapping. (7) A method of carrying an antibacterial composition comprising an inorganic or organic particle surface coated with an antibacterial metal by electroless plating on the inner tank surface of a carbon dioxide gas incubator. And so on. A carbon dioxide incubator carrying an antibacterial agent on the inner tank surface by other methods is also included in the present invention.

[0006] The antibacterial agent used in the present invention is an organic antibacterial agent (formaldehyde condensing bactericide, thiazolone, quaternary ammonium salt, phosphonium salt, pyridinium salt, halogen on the carbon to which the electron-withdrawing group is bonded). Compound having a bonded group, aromatic compound having a halogen group activated by an electron withdrawing group, compound having a carbon-carbon double bond or triple bond activated by a halogen atom, trihalomethylthio compound, thiocyanato compound ,
Benzimidazole derivatives, phenols, organic arsenic antibacterials, organic tin antibacterials, organic copper antibacterials, organic iodine antibacterials, isothiazoline antibacterials, pyrithione antibacterials, nitrile antibacterials, triazine antibacterials , Haloalkylthio antibacterial agents), paint antibacterial agents (haloallyl sulfone antibacterial agents, iodopropargyl antibacterial agents, N-haloalkylthio antibacterial agents, benzothiazole antibacterial agents, nitritolyl antibacterial agents, pyridine antibacterial agents, 8-oxyquinoline antibacterial agents, benzothiazole antibacterial agents, triazine and thiadiazine antibacterial agents, anilide antibacterial agents, adamantane antibacterial agents, dithiocarbide antibacterial agents, inorganic salt antibacterial agents, brominated indanone antibacterial agents, Benzyl brom acetate), inorganic antibacterial agents Preferred.

[0007] The amount of the antibacterial agent to be carried in the inner tank of the carbon dioxide gas incubator is not particularly limited, but it is meaningless if it is so small that no antibacterial activity is exhibited. Also, there is no point in having a large amount that is not economically acceptable. In view of these facts, the amount of the antibacterial agent carried in the inner tank of the carbon dioxide incubator is 1 to 7 times the total weight of the carrier such as a paint or a plate.
Preferably it is 0 weight percent.

[0008] The thickness of the antibacterial agent to be carried in the inner tank of the carbon dioxide gas incubator may be appropriately determined so that the desired antibacterial performance can be obtained within a range in which peeling does not easily occur. Further, as the type of the antibacterial agent to be carried in the inner tank of the carbon dioxide gas incubator, it is preferable to select an antibacterial agent which does not lose its antibacterial activity due to decomposition or the like during the carrying process.

The antibacterial agent to be carried in the inner tank of the carbon dioxide gas incubator is a material for supporting the antibacterial agent in the inner tank of the carbon dioxide gas incubator and, if necessary, additives such as a coloring agent and a material for maintaining appearance quality. May be included. Additives used together with the antibacterial agent to be supported in the inner tank of the carbon dioxide incubator include plasticizers (dioctyl phthalate, eposified soybean oil, cresol phosphate, butyl stearate, acetyl tributyl citrate, etc.), oxidation Inhibitors (dibutylhydroxytoluene, tofenlphosphite, mercaptobenzothiazole, etc.), stabilizers (metal soap, inorganic acid salt, urea, organic acid salt, etc.), UV absorbers (phenyl salicylate, benzotriazole, 2-hydroxy) Benzophenone, etc.),
Antistatic agents (N-acyl sarcosine, N, n-bis (2
-Hydroxyethyl) arylamine, etc.), flame retardants (chlorinated paraffin, bisphenol A tetrabromide, polybrominated biphenyl, bischloropropyl, etc.), coloring agents (titanium oxide, barium sulfate, calcium carbonate, carbon black, ultramarine, ben) Digii yellow, toluidine red, phthalocyanine blue, etc.), blowing agents (citric acid, adipic acid, azodicarboxylic amide, butane, pentane, difluorodichloromethane, N, N-dinitrosopentamethylenetetramine, etc.), activator, mold release (Polyorganosiloxane, paraffin, zinc montan wax, etc.), polymerization initiator (benzoyl peroxide, dicumyl peroxide, etc.), emulsifier (sodium alkylbenzene sulfonate, polyethylene glycol, etc.), emulsifier (bentonite, methyl) Cellulose, gelatin, and barium sulfate), filler (barium sulfate, calcium sulfate,
Clay, kaolin, silica, magnesium oxide, etc.) are conceivable.

The ultraviolet lamp installed in the inner tank of the carbon dioxide incubator can be installed on the ceiling, the side wall, or on each tray. Further, the ultraviolet lamp can be fixed and installed in advance, or a cordless ultraviolet lamp can be installed.

A light-shielding culture vessel used in a carbon dioxide incubator is used to protect cultured cells from ultraviolet rays emitted from an ultraviolet lamp installed in the carbon dioxide incubator. The light-shielding culture vessel is provided with a light-shielding property by coating a normal commercial culture vessel with a light-shielding paint or by packaging the light-shielding paint.

The disinfecting filter installed at the outlet of carbon dioxide in the inner tank of the carbon dioxide incubator can be obtained by carrying an antibacterial agent on the filter. As the antibacterial agent,
It is possible to carry an antibacterial agent of the same type as that carried on the inner tank of the carbon dioxide incubator. As a disinfecting filter that does not carry an antibacterial agent, a fine-grained filter that allows only carbon dioxide gas to pass (but does not allow bacteria and fungi to pass) can be considered.

[0013]

The present invention will be described in more detail with reference to examples.
The present invention is not limited at all by the examples.

Embodiment 1 -Application of non-aqueous acrylic resin paint to inner tank of carbon dioxide gas incubator-Non-aqueous acrylic resin paint (Daisosyl Vexel: Daiso) in inner tank including shelf plate of carbon dioxide incubator (CH-16M manufactured by Hitachi, Ltd.) Manufactured) is 300 g / m ^2.
And dried at 20 ° C./65 RH for one week and dried.

<< Embodiment 2. -Mold inoculation and culture inside the non-aqueous acrylic resin paint-coated carbon dioxide incubator-On the entire surface of each of the seven shelves inside the non-aqueous acrylic resin paint-coated carbon dioxide incubator prepared in Example 1 , Mold ( Aspergillus niger S-1, As
pergillus terreus S-3, Pen
icillumcitrinum S-5, Penic
illum funiculosum S-6, Cla
dsporioides S-8, Clioclazi
um virus S-10, Chaetomiu
m. lobosum S-11) is inoculated one by one (10 ⁸ cells / plate), and the ceiling, side walls and bottom surface, and the vats with water are mixed with each mold and inoculated with the same amount as the shelf. The cells were cultured for 3 months while supplying carbon dioxide at 37 ° C. At the time of mold cultivation, each shelf contains a complete nutrient solution 2 containing inorganic nutrients and sucrose.
The bacterial culture dish containing 0 ml was placed with the lid open. The complete nutrient solution was supplied as needed to keep 20 ml at all times. One month, two months, and three months later, shelves, ceilings, side walls and bottom surfaces, and a bat for watering were observed with a loupe. As a result, molds inoculated from any part of the carbon dioxide incubator were not detected at all after one month, two months, and three months (international standard IEC68-2-).
No growth of mold was observed by the method according to Method 2 of 10. ). In addition, a disinfecting filter carrying a phosphonium compound (tri-n-butyl-n-hexadecylphosphonium chloride: manufactured by Nippon Chemical Industry Co., Ltd.) was installed at the outlet of the carbon dioxide gas inside the carbon dioxide gas incubator.

Embodiment 3 -Sticking of titanium dioxide photocatalyst precipitation strengthened glass plate inside carbon dioxide gas incubator-Titanium sol is coated on a tempered glass (100cm x 100cm) by dip coating method, immersed in a simulated body fluid at 40 ° C for 48 hours, An apatite layer was deposited. The tempered glass plate was cut into an appropriate size and affixed to the ceiling, side, bottom, and shelf inside a carbon dioxide incubator (CH-16M manufactured by Hitachi, Ltd.).

<< Embodiment 4. -Inoculation and cultivation of mold inside the carbon dioxide gas incubator with titanium dioxide photocatalyst precipitation strengthened glass plate attached-Seven shelves each in the carbon dioxide incubator with titanium dioxide photocatalyst precipitation strengthened glass plate attached in Example 3 All over,
Mold ( Aspergillus niger S-1, A
spergillus terreus S-3, Pe
nicil - lum citrinum S-5, Pe
nicillum funicu - losum S-
6, Cladsporioides S-8, Clio
cla - dium virenus S-10, Cha
etomium globo - sum S-11)
Each type is inoculated (10 ⁸ cells / plate), and the ceiling, side walls and bottom surface, and the vats filled with water are mixed with each mold and inoculated in the same amount as the shelf, and carbon dioxide gas is supplied at 37 ° C. While illuminating the ultraviolet lamp, the cells were cultured for 3 months. During mold cultivation, each shelf contained 20 ml of a complete nutrient solution to which inorganic nutrients and sucrose were added.
The bacterial culture dish was placed with the lid open. The complete nutrient solution was supplied as needed to keep 20 ml at all times. One month, two months, and three months later, shelves, ceilings, side walls and bottom surfaces, and a bat for watering were observed with a loupe. As a result, 1
After 2 months, 2 months, and 3 months, no mold inoculated from any part of the carbon dioxide incubator was detected at all (by a method according to Method 2 of International Standard IEC68-2-10, No mold growth was observed.) At the outlet of the carbon dioxide gas inside the carbon dioxide gas incubator, a disinfecting filter carrying a pyridinium compound was installed.

Embodiment 5 FIG. -Inoculation and cultivation of bacteria into the inside of the carbon dioxide gas incubator with the titanium dioxide photocatalyst-precipitated glass plate attached thereon-Six shelves each of the inside of the carbon dioxide gas incubator with the titanium dioxide photocatalyst-precipitated glass plate attached prepared in Example 3 All over,
Escherichia coli ( Escherichia coli ), Pseudomonas aeruginosa ( Psudomonas aeruginosa ), Staphylococcus aureus ( Staphylococcus aur)
eus ), methicillin-resistant Staphylococcus aureus, Salmonella typhimuriu
m ), Bacillus cereus
Vibrio parahaemolyticus ( Vibrio pa - rahaemol)
yticus ) (10 ⁸ cells /
plate) and supply carbon dioxide gas at 37 ° C.
The ultraviolet lamp was turned on and the cells were cultured for 72 hours. After 72 hours, press a food stamp (manufactured by Nissui Pharmaceutical Co., Ltd .: for general bacteria) at any 10 places on each shelf and use a 37 ° C incubator for 24 hours.
Cultured for hours. After 24 hours of culture, the inoculum was not detected on the food stamp. At the outlet of the carbon dioxide gas inside the carbon dioxide gas incubator, a disinfecting filter carrying a silver-based antibacterial agent was installed.

Embodiment 6 FIG. »-Application of inorganic antibacterial agent to inside of carbon dioxide gas incubator-Inorganic antibacterial agent (Frescella: manufactured by Shinto V Ceramics Co., Ltd.)
Was mixed with a paint (manufactured by Cashew Co., Ltd.) so as to have a coating film concentration of 20 w%. Next, a carbon dioxide incubator (CH-CH, manufactured by Hitachi, Ltd.) was used with a Weider spray gun (manufactured by Iwata Paint Co., Ltd.).
16M) The inner ceiling, sides, bottom and shelf were spray-painted to a thickness of 10 micrometers.

Embodiment 7 -Inoculation and cultivation of mold inside carbon dioxide incubator with inorganic antibacterial applied to inner tank-Each of 7 in the carbon dioxide incubator prepared in Example 6 with inorganic antibacterial applied to inner tank Mold ( As)
pergillus niger S-1, Asper
gillus terreus S-3, Penici
llum citrinum S-5, Penicil
lum funiculosum S-6, Clads
porioides S-8, Cliocldium
virus S- 10, Chaetomium
globosum S-11) (10 inoculations)
⁸ cells / plate) The ceiling, side walls and bottom surface, and a water-filled bat are mixed with each mold and inoculated with the same amount as the shelf, while supplying carbon dioxide gas at 37 ° C.
Cultured for 3 months. At the time of mold cultivation,
20 ml of complete nutrient solution containing inorganic nutrients and sucrose
, The bacterial culture dish was placed with the lid open. The complete nutrient solution was supplied as needed to keep 20 ml at all times. One month, two months, and three months later, shelves, ceilings, side walls and bottom surfaces, and a bat for watering were observed with a loupe. As a result, no mold inoculated from any part of the inside of the carbon dioxide incubator was detected at all after one month, two months, and three months (according to method 2 of International Standard IEC68-2-10). No mold growth was observed by the method described above.) At the outlet of the carbon dioxide in the carbon dioxide incubator, a disinfecting filter carrying an inorganic antibacterial agent (Frescella) was installed.

Embodiment 8 FIG. -Application of phosphonium compound inside carbon dioxide incubator-Phosphonium compound (tri-n-butyl-n-hexadecylphosphonium chloride: manufactured by Nippon Chemical Industry Co., Ltd.) and DOP
Was mixed with a paint (manufactured by Cashew Co.) so that the coating film concentration was 30% by weight. Next, 10 parts were applied to the ceiling, side, bottom, and shelf inside the carbon dioxide incubator (CH-16M, manufactured by Hitachi, Ltd.) using a Weider spray gun (manufactured by Iwata Paint Co., Ltd.).
It was spray-painted to a thickness of a micrometer.

Embodiment 9 -Mold Inoculation and Culture into Inside of Carbon Dioxide Incubator with Inner Tank Coated with Phosphonium Compound-Seven shelves of each of the insides of the carbon dioxide incubator prepared with the inner tank coated with phosphonium compound prepared in Example 8 Mold ( Aspergillus niger S-1, As)
pergillus terreus S-3, Pen
icllumcitrinum S-5, Penic
llum funiculosum S-6, Clads
porioides S-8, Cliocldium
virus S-10, Chaetomium
globosum S-11) (10 inoculations)
⁸ cells / plate) The ceiling, side walls and bottom surface, and a water-filled bat are mixed with each mold and inoculated with the same amount as the shelf, while supplying carbon dioxide gas at 37 ° C.
Cultured for 3 months. At the time of mold cultivation,
20 ml of complete nutrient solution containing inorganic nutrients and sucrose
, The bacterial culture dish was placed with the lid open. The complete nutrient solution was supplied as needed to keep 20 ml at all times. One month, two months, and three months later, shelves, ceilings, side walls and bottom surfaces, and a bat for watering were observed with a loupe. As a result, no mold inoculated from any part of the inside of the carbon dioxide incubator was detected at all after one month, two months, and three months (according to method 2 of International Standard IEC68-2-10). No mold growth was observed by the method described above.) At the outlet of the carbon dioxide in the carbon dioxide incubator, a disinfecting filter carrying an inorganic antibacterial agent (Frescella) was installed.

Embodiment 10 FIG. -Culture of animal cells inside the carbon dioxide gas incubator with titanium dioxide photocatalyst deposition-enhanced glass plate attached-On the shelf installed in the carbon dioxide gas incubator with titanium dioxide photocatalyst-deposited glass plate attached prepared in Example 3, 37 V79 cells (for cytotoxicity test) were used in two commercially available cell culture flasks having a capacity of 10 ml (manufactured by Becton Dickinson: one of the two flasks was light-shielded with aluminum foil). While supplying carbon dioxide at ゜ C,
The ultraviolet lamp was turned on and the cells were cultured for 72 hours. After 72 hours, V79 cells were observed under a microscope. As a result, the V79 cells cultured in the flask protected from light by the aluminum foil survived and proliferated, but all the V79 cells cultured in the flask protected from the light died.

<< Comparative Example 1. »-Inoculation and cultivation of mold inside the CO2 incubator without antibacterial treatment in the inner tank-7 each in the CO2 incubator without antibacterial treatment in the inner tank
Mold on the whole shelf ( Aspergillus ni)
ger S-1, Aspergillus terre
us S-3, Penicillum citrinu
m S-5, Penicillum funiculo
sum S-6, Cladsporoides S-
8, Cliocladium virus S-1
0, Chaetomium globosum S-
11) inoculated one by one (10 ⁸ cells / plat)
e) In addition, each mold was mixed and inoculated in the ceiling, the side wall and the bottom surface, and the vats filled with water to the same amount as the shelves, and cultured for 3 months while supplying carbon dioxide gas at 37 ° C. . At the time of mold culture, a bacterial culture dish containing 20 ml of a complete nutrient solution containing inorganic nutrients and sucrose was placed on each shelf with the lid open. The complete nutrient solution was supplied as needed to keep 20 ml at all times. One month, two months, and three months later, shelves, ceilings, side walls and bottom surfaces, and a bat for watering were observed with a loupe. As a result, one month, two months, and three months later, the inoculated mold was detected from the entire surface of the carbon dioxide incubator (International Standard IEC68-).
Mold growth was observed by a method similar to Method 2 of 2-10. ). Note that a disinfecting filter was not installed at the outlet of the carbon dioxide gas inside the carbon dioxide gas incubator.

<< Comparative Example 2. »-Inoculation and cultivation of bacteria inside the carbon dioxide incubator without antibacterial treatment in the inner tank-6 each in the carbon dioxide incubator without antibacterial treatment in the inner tank
E. coli ( Escherichia c)
oli ), Pseudomonas aerug ( Psudomonas aerug )
inosa ), Staphylococcus aureus ( Staphyloco)
ccus aureus), methicillin-resistant Staphylococcus aureus, Salmonella (Salmo - nella typ
himurium ), Bacillus cereus ( Bacillus )
cereus ) Vibrio parahaemolyticus ( Vibrio para )
haemolyti - cus ) (1 each)
0 ⁸ cells / plate) and, while supplying carbon dioxide gas at 37 ° C, turns off the UV lamp, and cultured for 72 hours. After 72 hours, a food stamp (manufactured by Nissui Pharmaceutical Co., Ltd .: for general bacteria) was pressed at any 10 places on each shelf, and cultured in an incubator at 37 ° C for 24 hours. After culturing for 24 hours, all of the inoculated bacteria were detected on the food stamp.
Note that a disinfecting filter was not installed at the outlet of the carbon dioxide gas inside the carbon dioxide gas incubator.

[0026]

According to the present invention, an inner tank is provided by a carbon dioxide incubator characterized by carrying an antibacterial agent in the inner tank and a carbon dioxide incubator characterized by installing an ultraviolet lamp in the inner tank. Bacteria and fungi can be prevented from multiplying, and as a result, there is no possibility of bacterial or fungal contamination occurring in a flask or a petri dish used for culturing cells. In addition, the cultured cells can be protected from ultraviolet rays by the light-shielding culture container according to the present invention.

Claims (9)

[Claims] 1. A carbon dioxide incubator having an antibacterial and antifungal agent carried on the entire inner tank (ceiling, shelf, shelf support, side surface, internal door, bottom, container for water). 2. The carbon dioxide incubator according to claim 1, wherein the loading of the antibacterial and antifungal agent is carried out by any one of kneading, painting, printing, hot stamping, sticking, and plating. . 3. An antibacterial agent to be carried, comprising an organic antibacterial antifungal agent (formaldehyde condensing bactericide, thiazolone, quaternary ammonium salt, phosphonium salt, pyridinium salt,
A compound in which a halogen group is bonded to the carbon to which the electron-withdrawing group is bonded, an aromatic compound having a halogen group activated by the electron-withdrawing group, a carbon-carbon double bond or triple activated by a halogen atom Compounds having bonds, trihalomethylthio compounds, thiocyanato compounds, benzimidazole derivatives, phenols, organic arsenic antibacterial agents,
Organotin antibacterial agents, organocopper antibacterial agents, organic iodine antibacterial agents, isothiazoline antibacterial agents, pyrithione antibacterial agents, nitrile antibacterial agents, triazine antibacterial agents, haloalkylthio antibacterial agents), paint antibacterial agents ( Haloallyl sulfone antibacterial agent, iodopropargyl antibacterial agent, N-haloalkylthio antibacterial agent, benzthiazole antibacterial agent, nitritolyl antibacterial agent, pyridine antibacterial agent, 8-oxyquinoline antibacterial agent, benzothiazole antibacterial agent , Triazine and thiadiazine antibacterial agents, anilide antibacterial agents, adamantane antibacterial agents, dithiocarbide antibacterial agents, inorganic salt antibacterial agents, brominated indanone antibacterial agents, benzyl bromacetate), and inorganic antibacterial agents The carbon dioxide incubator according to claim 1, wherein the carbon dioxide incubator is selected from a group. 4. An antibacterial and antifungal agent loaded with additives (plasticizer, antioxidant, stabilizer, ultraviolet absorber, antistatic agent,
The carbonic acid according to claim 1, wherein the carbonic acid is used together with at least one of a flame retardant, a colorant, a pigment, a foaming agent, an activator, a release agent, a polymerization initiator, an emulsifier, an emulsion stabilizer, and a filler. Gas incubator. 5. The carbon dioxide incubator according to claim 1, wherein an ultraviolet lamp is provided in the inner tank. 6. A light-shielding culture vessel for use in the carbon dioxide incubator according to claim 5. 7. A method for culturing cells using the carbon dioxide incubator according to claim 1. 8. A method for culturing cells using the light-shielding culture container according to claim 6. 9. The carbon dioxide incubator according to claim 1, wherein a disinfecting filter is provided at an inlet of the carbon dioxide in the inner tank.