JP6293389B2 - Microbicide control agent and acaricide composition - Google Patents

Description

  The present invention relates to a microbial control agent, an antibacterial agent, an algal control agent, a biofilm formation inhibitor, a microbial control method, an antibacterial method, an algae control method, a biofilm formation suppression method, an acaricide composition, an acaricide member and an acaricidal method. About.

  Techniques for removing microorganisms and mites adhering to food, water, daily necessities, animals, plants, sludge, etc. are extremely important from the viewpoint of environmental hygiene and durability. Conventionally, in air purification technology, water treatment technology, plant factories, food industry, methods for controlling microorganisms by filtration membranes such as microfiltration membranes and reverse osmosis membranes, metals such as silver, copper, zinc, antibiotics or Removal of microorganisms is performed by a method using an antibacterial substance such as an organic compound typified by agricultural chemicals such as imidazole and thiazole. As an example of these, a technique has been proposed in which an antibacterial effect is imparted to a fiber product by bringing the liquid containing silver (including at least one of silver ions and silver colloid particles) into contact with the fiber product and removing the liquid component. (For example, see Patent Document 1).

JP 2007-31857 A

  However, in the method of controlling the amount of microorganisms using the above-described filtration membrane, problems such as high pressure loss and clogging occur, and it is difficult to efficiently remove microorganisms and the like. In addition, in the technique of performing antibacterial action using silver ions as disclosed in Patent Document 1, there is a problem in that silver ions are eluted and there is a problem in the durability of the antibacterial effect, and the environment is affected by the elution of silver ions. was there. In addition, the microorganism control agent using such silver ions has been required to further improve the antibacterial performance.

  The present invention has been made in view of the above, and provides a microbial control agent that is excellent in microbial control performance, in particular, antibacterial performance, algal control performance and biofilm formation suppression performance, and in which metal elution is suppressed. With the goal. Moreover, this invention is excellent in the performance which suppresses generation | occurrence | production and increase of a tick, and it aims at providing an acaricide composition, an acaricide member, and an acaricidal method.

  As a result of intensive studies to achieve the above object, the present inventor has found that the above object can be achieved by a combination of specific metal particles, and has completed the present invention.

That is, the present invention includes, for example, the subject matters described in the following sections.
Item 1. A microbial control agent comprising platinum particles and metal particles other than platinum.
Item 2. Item 2. The microorganism control agent according to Item 1, wherein the metal particles include at least one selected from the group consisting of silver particles, copper particles, nickel particles, and zinc particles.
Item 3. Item 2. The microorganism control agent according to Item 1, wherein the metal particles include at least one selected from the group consisting of silver particles and copper particles.
Item 4. Item 4. The microorganism control agent according to any one of Items 1 to 3, wherein an average particle size of the platinum particles and the metal particles is 0.1 to 1000 nm.
Item 5. Item 5. The microorganism control agent according to any one of Items 1 to 4, wherein the platinum particles and the metal particles are supported on a substrate.
Item 6. Microbial control agent according to said platinum particles and the metal particles are supported on the substrate in 0.01~20,000ng / cm ^2, Item 5.
Item 7. Item 7. The microorganism control agent according to Item 5 or 6, wherein the substrate is formed of a resin.
Item 8. The microorganism control according to Item 7, wherein the resin includes at least one selected from the group consisting of polystyrene resin, vinyl chloride resin, fluorine resin, ABS resin, PET resin, polycarbonate resin, urea resin, olefin resin, and phenol resin. Agent.
Item 9. Any of the above items 5 to 8, wherein the substrate is formed into any one of a sheet shape, a plate shape, a block shape, a net shape, a punching sheet shape, a granular shape, a rod shape, a crushed shape, and a dish shape. The microorganism control agent according to Item 1.
Item 10. Item 10. An antibacterial agent comprising the microorganism control agent according to any one of items 1 to 9.
Item 11. The algae control agent containing the microorganism control agent of any one of said items 1-9.
Item 12. A biofilm formation inhibitor comprising the microorganism control agent according to any one of Items 1 to 9.
Item 13. A microorganism control method for imparting a microorganism control function to an object using the microorganism control agent according to any one of Items 1 to 9.
Item 14. The antibacterial method of giving antimicrobial property to a target object using the microorganism control agent of any one of said items 1-9.
Item 15. An algae control method for imparting alga control to an object using the microorganism control agent according to any one of items 1 to 9.
Item 16. The biofilm formation suppression method of giving a biofilm formation inhibitory effect to a target object using the microorganism control agent of any one of said items 1-9.
Item 17. An acaricidal composition comprising platinum particles and metal particles other than platinum.
Item 18. Item 18. The acaricidal composition according to Item 17, wherein the metal particles include at least one selected from the group consisting of silver particles, copper particles, nickel particles, and zinc particles.
Item 19. Item 19. The acaricidal composition according to Item 17 or 18, wherein the metal particles include at least one selected from the group consisting of silver particles and copper particles.
Item 20. Item 20. The acaricidal composition according to any one of Items 17 to 19, wherein an average particle size of the platinum particles and the metal particles is 0.1 to 1000 nm.
Item 21. The tick-proof member (however, a beekeeping member is excluded) using the acaricide composition of any one of said item | term 17-20.
Item 22. 21. A tick-proof method for imparting an anti-mite function to an object (excluding beekeeping members) using the acaricidal composition according to any one of items 17 to 20.

  The microbial control agent according to the present invention contains platinum particles and metal particles other than platinum, so that the microbial control performance, especially antibacterial performance, anti-algae performance, Excellent formation suppression performance, and metal elution is also suppressed. Therefore, the above-mentioned microbial control agent exhibits excellent microbial control performance, in particular, antibacterial property, anti-algae performance and biofilm formation suppression performance, and also improves durability and sustainability by suppressing metal elution. The impact on the environment can also be reduced. In addition, by treating the substrate with the microorganism control agent according to the present invention, three different effects of an antibacterial effect, an algal control effect, and a biofilm formation inhibitory effect can be obtained together. By using the microorganism control agent according to the present invention, the processing effort can be halved and the processing cost can be reduced, which is very useful.

  The acaricidal composition according to the present invention includes a platinum particle and a metal particle other than platinum, thereby exhibiting a tick generation suppressing function and a tick increase suppressing function that cannot be achieved with platinum particles and the above metal particles alone. It becomes possible to do. The mite-preventing member using the mite-killing composition is excellent in the performance of suppressing the occurrence and increase of mites, so it can be used for all materials that require mite-prevention and mite-killing, for example, as life-related materials, Suitable for tatami, carpets, wall materials, floor materials, ceiling materials, furniture, home appliances, food containers, food packaging materials, kitchen products, etc. Or as a member to be stored (excluding beekeeping members).

It is the result of the reference test example 1, and has shown the relationship between breeding time and the number of living honeybee mites. It is the result of the reference test example 2, and has shown the relationship between breeding time and the number of living honeybee mites.

  Hereinafter, embodiments of the present invention will be described in detail.

(Microbicide control agent and acaricide composition)
The microorganism control agent and acaricide composition of this embodiment contain platinum particles and metal particles other than platinum. Hereinafter, the above “metal particles other than platinum” may be simply abbreviated as “metal particles”.

  The platinum particles are particles composed of platinum (Pt). The platinum particles are usually formed of platinum element, but may contain platinum oxide. In addition, the platinum particles may contain an alloy of platinum and another metal element.

  The metal particles are not particularly limited, but are preferably at least one selected from the group consisting of silver particles, copper particles, nickel particles, and zinc particles. In this case, since the toxicity is low and the antibacterial performance and the acaricidal performance are excellent, the effect of preventing or removing the generation of microorganisms and the effect of suppressing the generation and increase of mites are increased.

  The metal particles are usually formed of at least one metal element selected from the group consisting of silver particles, copper particles, nickel particles, and zinc particles, for example, like the platinum particles. The metal particles may contain an oxide of the metal element. In addition, the metal particles may include an alloy composed of the metal element and another metal element.

  For example, the metal particles may be composed of only one of silver particles, copper particles, nickel particles, and zinc particles, or may be composed of two or more.

  The metal particles preferably include at least one selected from the group consisting of silver particles and copper particles. In this case, since the antibacterial performance is particularly excellent, the effect of preventing or removing the generation of microorganisms is further increased. The metal particles are particularly preferably silver particles. In this case, in addition to excellent microbial control performance and acaricidal performance, metal particles with high safety are used and the above effects are exhibited at an extremely low concentration. And irritation are extremely low.

  The average particle diameter of the platinum particles and the metal particles is not particularly limited, and can be, for example, nanoparticles of 0.1 nm or more, for example, 1000 nm or less. In this case, the antibacterial performance and the effect of acaricide are easily exhibited, and when the platinum particles and the metal particles are supported on the base material, the platinum particles and the metal particles are easily supported on the base material. . In addition, when the particle diameter is 0.1 nm or more, these metals can be easily formed into fine particles, and when the particle diameter is 1000 nm or less, the liquid dispersion or the aqueous dispersion for supporting the carrier It is easy to suppress sedimentation of metal particles in the production process, and handling is difficult. In addition, the average particle diameter of platinum particle | grains and the said metal particle here means the result measured with the zeta potential measuring device (Zeta Sizer nano ZS90, product made by Malvern).

  The shapes of the platinum particles and the metal particles are not particularly limited, and examples thereof include spherical particles, polygonal particles, fibrous particles, needle-like particles, flake-like particles, and porous particles. The platinum particles and the metal particles may be in an aggregated state.

  In the microbial control agent and the acaricide composition, the content of the platinum particles and the metal particles is not limited. From the viewpoint that the antibacterial performance and the acaricidal performance of the microbial control agent are further increased, the platinum particles and the metal particles have a content ratio of 100 when the total mass of the platinum particles and the metal particles is 100. The number of platinum particles is 95 or less, preferably 90 or less, more preferably 80 or less, and particularly preferably 70 or less, preferably 10 or more, more preferably 20 or more, and particularly preferably 30 or more.

  The form of the microbial control agent and the acaricide composition of the present embodiment is not particularly limited as long as it contains platinum particles and the metal particles. For example, the microorganism control agent and the acaricide composition may be in a form (dispersion) in which platinum particles and the metal particles are dispersed in a medium. In this case, the medium is usually a liquid, and water, lower alcohol, or a mixed solvent thereof is exemplified.

  In the microbial control agent and the acaricide composition of this embodiment, platinum particles and the metal particles may be supported on a substrate. In this case, a simple method (for example, immersion in a liquid and microwave treatment described later) may impart antibacterial properties, algae-proofing properties, biofilm formation inhibition performance, acaricidal and anti-mite functions, etc. to the substrate. it can. In the microorganism controlling agent and the acaricidal composition, the form in which the platinum particles and the metal particles are supported on the base material may be referred to as “microorganism controlling member”.

  The type of substrate is not particularly limited. From the viewpoint that the platinum particles and the metal particles are more easily supported and can be easily applied as a microbial control agent for various products, the base material is preferably formed of a resin.

  When the substrate is formed of a resin, the resin is made of polystyrene resin, vinyl chloride resin, fluorine resin, ABS resin, PET resin, polycarbonate resin, urea resin, polylactic acid resin, olefin resin, and phenol resin. It is preferable to include at least one selected from the group. When the base material is formed by including any of these resins, the platinum particles and the metal particles are easily carried on the base material, and high antibacterial performance and acaricidal performance can be exhibited. Examples of the fluororesin include PTFE (polytetrafluoroethylene). Examples of the olefin resin include polyethylene and polypropylene resin.

  When the substrate is formed of a resin, the polycarbonate resin is a metal particle to be used because the polycarbonate resin exhibits a sufficient microbial control effect and acaricidal effect even if the supported amount of platinum particles and the metal particles is small. This is preferable in terms of economic efficiency.

The amount of platinum particles and metal particles supported on the substrate is not limited, but may be, for example, 0.001 ng / cm ² or more, preferably 0.01 ng / cm ² or more, more preferably 0.00. and at 1 ng / cm ² or more, and is, for example, be a 100,000ng / cm ² or less, preferably 50,000ng / cm ² or less, more preferably the group in an amount of 20,000 ng / cm ² or less It is preferable to be carried on a material. By supporting the platinum particles and the metal particles on the substrate in such a loading amount, the antibacterial performance of the microorganism control agent and the acaricidal performance of the acaricide composition can be sufficiently exhibited. In addition, the carrying amount (ng / cm ² ) on the substrate referred to in this specification is the total carrying amount of the carrying amount of platinum particles (ng / cm ² ) and the carrying amount of the metal particles (ng / cm ² ). Indicates.

  Examples of the shape of the substrate include a sheet shape, a plate shape, a block shape, a net shape, a punching sheet shape, a granular shape, a rod shape, a crushed shape, a dish shape, and various product shapes according to applications. If it is any of these shapes, the range which can apply a microbe control agent and an acaricide composition will become wider, and it will become easy to provide antibacterial performance and acaricide performance. For example, if the shape of the base material is a sheet shape, the thickness is not limited at all, and the substrate can be formed to a thickness according to the application. The base material may have pores inside such as a hollow shape or a porous shape.

  In the microorganism control agent and the acaricide composition of the present embodiment, other materials may be added as long as the effects of the present invention are not inhibited. Other materials include, for example, platinum particles and particles composed of metal elements other than the metal particles, other microorganism control agents (antibacterial agents, bactericides, antiseptics, algae control agents, fungicides, etc.), pH Examples thereof include regulators, antioxidants, light stabilizers, antifoaming agents, dispersion stabilizers, and lubricants.

  Examples of the other microorganism control agents include, for example, isothiazoline compounds, benzimidazole compounds, triazole compounds, nitroalcohol compounds, dithiol compounds, thiophene compounds, haloacetylene compounds, phthalimide compounds, haloalkylthio compounds. , Phenylurea compounds, triazine compounds, guanidine compounds, quaternary ammonium salt compounds, and the like.

  Specific examples of the isothiazoline-based compound include 2-methyl-4-isothiazolin-3-one, 2-n-octyl-4-isothiazolin-3-one, and 5-chloro-2-methyl-4-isothiazoline-3. -One, 5-chloro-2-n-octyl-4-isothiazolin-3-one, 4-chloro-2-n-octyl-4-isothiazolin-3-one, 4,5-dichloro-2-n-octyl -4-isothiazolin-3-one, 2-methyl-4,5-trimethylene-4-isothiazolin-3-one, 1,2-benzisothiazolin-3-one, Nn-butyl-1,2-benzisothiazoline -3-one etc. are mentioned.

  Specific examples of the benzimidazole compound include methyl-2-benzimidazole carbamate (common name: carbendazim), ethyl-2-benzimidazole carbamate, 2- (4-thiazolyl) benzimidazole, and the like.

  Specific examples of triazole compounds include α-butyl-α- (2,4-dichlorophenyl) -1H-1,2,4-triazole-1-ethanol (common name: hexaconazole), α- [ 2- (4-Chlorophenyl) ethyl] -α- (1,1-dimethylethyl) -1H-1,2,4-triazole-1-ethanol (common name: tebuconazole), and α- (4-chlorophenyl)- α- (1-cyclopropylethyl) -1H-1,2,4-triazole-1-ethanol (common name: cyproconazole), 1-[[2- (2,4-dichlorophenyl) -4-n- Propyl-1,3-dioxolan-2-yl] methyl] -1H-1,2,4-triazole (common name: propiconazole) and the like.

  Specific examples of the nitroalcohol compound include 2-bromo-2-nitropropane-1,3-diol, 2,2-dibromo-2-nitro-1-ethanol, and the like.

  Specific examples of the dithiol-based compound include 4,5-dichloro-1,2-dithiol-3-one.

  Specific examples of the thiophene compound include 3,3,4-trichlorotetrahydrothiophene-1,1-dioxide, 3,3,4,4-tetrachlorotetrahydrothiophene-1,1-dioxide and the like.

  Specific examples of the haloacetylene compound include N-butyl-3-iodopropiolic acid amide, 3-iodo-2-propynyl-N-butylcarbamate, and the like.

  Specific examples of the phthalimide-based compound include N-1,1,2,2-tetrachloroethylthio-tetrahydrophthalimide (Captafol), N-trichloromethylthio-tetrahydrophthalimide (Captan), and N-dichlorofluoromethylthiophthalimide (Fluorolpet). N-trichloromethylthiophthalimide (Folpet) and the like.

  Specific examples of the haloalkylthio compounds include N-dimethylaminosulfonyl-N-tolyl-dichlorofluoromethanesulfamide (Tolylfluoride), N-dimethylaminosulfonyl-N-phenyl-dichlorofluoromethanesulfamide (Dichlofluoride). ) And the like.

  Specific examples of the phenylurea compound include 3- (3,4-dichlorophenyl) -1,1-dimethylurea.

  Specific examples of the triazine compound include 2-methylthio-4-t-butylamino-6-cyclopropylamino-s-triazine.

  Specific examples of the guanidine-based compound include 1,6-di- (4'-chlorophenyldiguanide) -hexane, polyhexamethylene biguanidine hydrochloride and the like.

  Specific examples of the quaternary ammonium salt compound include hexadecyltrimethylammonium bromide, hexadecyltrimethylammonium chloride, benzalkonium chloride, di-n-decyl-dimethylammonium chloride, 1-hexadecylpyridinium chloride and the like. It is done.

  These other microorganism control agents can be used alone or in combination of two or more as required. Preferably, it is one or two or more compounds selected from the group consisting of benzimidazole compounds, triazole compounds, haloacetylene compounds and salts thereof, particularly preferably methyl-2-benzimidazole carbamate, 2- (4-thiazolyl) benzimidazole, α- [2- (4-chlorophenyl) ethyl] -α- (1,1-dimethylethyl) -1H-1,2,4-triazole-1-ethanol, α- One or two selected from the group consisting of butyl-α- (2,4-dichlorophenyl) -1H-1,2,4-triazole-1-ethanol and 3-iodo-2-propynyl-N-butylcarbamate It is.

  In addition, when other microbial control agents are included, the blending ratio is not particularly limited and is appropriately selected depending on the dosage form, purpose and application. For example, the total amount of platinum particles and metal particles other than platinum is 100 parts by weight. The total amount of the microorganism control agent is 1 to 10000 parts by weight, preferably 10 to 1000 parts by weight.

  Examples of the pH adjuster include acids and bases. Specific examples of the acid include hydrochloric acid, nitric acid, sulfuric acid, lactic acid, acetic acid, citric acid and the like. Specific examples of the base include sodium hydroxide, potassium hydroxide, sodium carbonate, triethylamine, trimethylamine, ammonia and the like. These pH adjusters can be used individually by 1 type, or can be used in mixture of 2 or more types as needed.

  Examples of the antioxidant include phenolic antioxidants and amine antioxidants. Specific examples of the phenolic antioxidant include 2,6-di-t-butyl-4-methylphenol and 2,2'-methylenebis (4-methyl-6-t-butylphenol). Specific examples of amine-based antioxidants include alkyldiphenylamine and N, N′-di-s-butyl-p-phenylenediamine. These antioxidants can be used individually by 1 type, or can be used in mixture of 2 or more type as needed.

  Examples of the light stabilizer include hindered amine light stabilizers such as bis (2,2,6,6-tetramethyl-4-piperidyl) sebacate. A light stabilizer can be used individually by 1 type, or can mix and use 2 or more types as needed.

  As an antifoamer, a silicone type antifoamer, an organic type antifoamer, etc. are mentioned, for example. Specific examples of the organic antifoaming agent include surfactants, polyethers and higher alcohols. An antifoamer can be used individually by 1 type, or can mix and use 2 or more types as needed.

  The method for producing the microbial control agent and the acaricide composition is not particularly limited. For example, when the microbial control agent and the acaricidal composition are in a form in which platinum particles and the metal particles are dispersed in the above-mentioned medium, platinum particles, the metal particles and the medium are prepared and mixed in a predetermined blending amount. Thus, a microbial control agent and an acaricidal composition can be prepared. In preparing the microbial control agent and the acaricide composition, a commercially available mixer or disperser may be used as appropriate. In the case of such a microorganism control agent and acaricide composition, the total amount of platinum particles and the metal particles is 0.00001 parts by weight or more, preferably 0.0001 parts by weight with respect to 100 parts by weight of the medium. Part or more, more preferably 0.001 part by weight or more, for example, 50 parts by weight or less, preferably 10 parts by weight or less, more preferably 1 part by weight or less. In this case, the mixing ratio of the platinum particles and the metal particles (the mass of the platinum particles: the mass of the metal particles) is, for example, 5 or more when the total mass of the platinum particles and the metal particles is 100. The number is preferably 10 or more, more preferably 20 or more, particularly preferably 30 or more, and for example, 95 or less, preferably 90 or less, more preferably 80 or less, and particularly preferably 70 or less.

  On the other hand, the method for producing a microbial control agent and an acaricidal composition in which platinum particles and the metal particles are supported on a substrate is not particularly limited. For example, a dispersion of platinum particles and the metal particles is prepared in advance, and the platinum particles and the metal particles are supported on the substrate by using the dispersion to support the platinum particles and the metal particles on the substrate. A microbial control agent and an acaricide composition which are supported can be produced. Examples of the method for supporting the platinum particles and the metal particles from the dispersion on the substrate include a method of immersing the substrate in the dispersion, a method of spraying or applying the dispersion to the substrate, a vapor deposition method, and the like. However, it is not limited to these.

  As described above, when producing a microbial control agent and an acaricide composition by a method of immersing a substrate in the dispersion, by irradiating microwaves in a state where the substrate is immersed in the dispersion. The platinum particles and the metal particles may be fixed to the base material. Similarly, when producing a microorganism control agent and an acaricide composition by spraying or applying the dispersion to the substrate, microwaves are irradiated after forming a coating of the dispersion by spraying or application. Thus, the platinum particles and the metal particles may be fixed to the substrate.

  By irradiating with microwaves as described above, uniform coating can be achieved in a relatively short time, and the support of the platinum particles and the metal particles on the substrate becomes stronger, and platinum can be applied to the substrate without using a binder. It becomes possible to carry the particles and the metal particles. Thereby, it becomes possible for a microorganism control agent and an acaricide composition to exhibit the more excellent antibacterial effect and acaricide effect. In addition, since the platinum particles and the metal particles are more firmly supported on the base material, there is an advantage that elution of the metal element to the outside is further suppressed.

In the case of irradiation with microwaves, the irradiation intensity can be 0.005 to 0.1 W / cm ³ . For microwave irradiation, for example, a commercially available microwave irradiation apparatus may be used, or a microwave oven may be used. Moreover, when irradiating a microwave, you may heat suitably. What is necessary is just to set the irradiation time of a microwave suitably according to the irradiation intensity | strength of a microwave, for example, can be 0.1 to 60 minutes.

  As described above, the microorganism control agent and the acaricide composition of the present embodiment are composed of platinum particles and the metal particles, and are excellent in antibacterial performance and acaricide performance.

  For example, in the microorganism control agent of this embodiment, each antibacterial activity value against S. aureus and Escherichia coli in an antibacterial test according to JIS Z2801 can be 2.0 or more.

  The antibacterial performance can be evaluated in accordance with the above JIS Z2801, and a generally known film adhesion method can be employed. In this film contact method, a liquid containing bacteria is dropped on the surface of a measurement sample, and the liquid is covered with a film. After 24 hours, bacteria are collected from the sample, and the number of viable bacteria is measured. The measurement result is compared with the number of viable bacteria in the unprocessed product to calculate the antibacterial activity value.

  In addition to the film adhesion method, a shake method may be employed. In this method, the sample is immersed in a suspension of bacteria and shaken to bring the bacteria into contact with the sample and measure the number of viable bacteria. The measurement result is compared with the number of viable bacteria in the unprocessed product to calculate the antibacterial activity value. Such a shake method can be adopted for a sample to which the film adhesion method cannot be applied.

  In the microorganism control agent of the present embodiment, each antibacterial activity value against S. aureus and Escherichia coli measured by the film adhesion method can be 2.0 or more.

  The microorganism control agent (dispersion liquid containing platinum particles and metal particles other than platinum) according to the present embodiment is, for example, an antibacterial treatment and sterilization of a container for culturing cells such as a petri dish (hereinafter referred to as “cell culture container”). Can be used for processing.

  The cell culture container surface-treated with the microorganism control agent of this embodiment is excellent in antibacterial performance. Therefore, conventionally, as a pretreatment for culturing cells, it has been necessary to carry out antibacterial treatment by a method such as heating or ultraviolet irradiation of a cell culture container. When the cell culture container is used, the cells can be cultured without such pretreatment, so that it is possible to save time and labor for the pretreatment.

  Further, even when a cell culture container subjected to antibacterial treatment or the like with the microorganism control agent of the present embodiment is used, cells can be cultured without affecting cell proliferation.

  In addition, by using a cell culture container that has been antibacterial treated with the microorganism control agent of the present embodiment, it is possible to prevent other bacteria from being mixed before and after culturing and during culturing. It is difficult and can culture cells efficiently. That is, it is possible to omit the addition of antibiotics used to prevent contamination of bacteria during cultivation.

  The cell culture container may be subjected to antibacterial treatment or the like with the microorganism control agent of this embodiment immediately before culturing the cells. Alternatively, after the cell culture container is produced or simultaneously with the production of the cell culture container, an antibacterial treatment or the like may be performed with the microorganism control agent of the present embodiment. Further, the antibacterial treatment is not easily affected by heat treatment or ultraviolet irradiation treatment.

  Various types of cells can be cultured in the cell culture container that has been antibacterial treated with the microorganism control agent of the present embodiment. The type of cell is not limited.

  The microorganism control agent of this embodiment has algae generation suppression performance, and the effect can be visually confirmed by a test in a plant cultivation container as shown in Examples described later.

  The microorganism control agent of the present embodiment has a biofilm formation inhibitory performance, and it can be confirmed that biofilm formation is remarkably small (a level that cannot be visually confirmed at all) by a test using a sample plate as shown in Examples described later. Biofilms are also called biofilms or slimes. Generally, microorganisms adhere to and grow on the surface of substances in the aqueous system to produce high-molecular substances such as polysaccharides, proteins, and nucleic acids from inside microbial cells. A product produced by a microorganism is a structure. Biofilm formation can cause significant adverse effects in various industries due to contamination and corrosion of equipment and flow paths (pipelines, dredging, etc.), system blockages, product defects, and energy loss.

  The acaricidal composition of this embodiment has acaricidal and acaricidal performance, and as shown in the examples described later, the growth of mites is achieved by treating the substrate formed of resin or the like with the acaricidal composition. Can be suppressed. Thus, the acaricide composition of this embodiment can provide an acaricidal function to an object.

  In the microorganism control agent and the acaricide composition of the present embodiment, since the platinum particles and the metal particles are included, the antibacterial performance and the acaricide performance are excellent. In the above, even if the platinum particles and the metal particles have a low concentration, good antibacterial performance and acaricidal performance can be exhibited. Therefore, as described above, the microbial control agent and the miticide composition in which the platinum particles and the metal particles are supported on the base material have high antibacterial performance and high algal control performance even if the supported amount is smaller than before. It is possible to develop high biofilm formation inhibiting ability and acaricidal performance. Especially when the metal particles contain at least one selected from the group consisting of silver particles, copper particles, nickel particles and zinc particles, antibacterial performance and acaricidal performance are excellent, even if the loading amount is smaller, High antibacterial performance, high antialgae performance, high biofilm formation suppression capability and high acaricidal performance are manifested. That is, by treating the substrate with the microorganism control agent or the acaricide composition according to the present invention, it is possible to obtain four different effects of the antibacterial effect, the algae control effect, the biofilm formation inhibitory effect, and the acaricide effect. . In addition, these metal particles have the advantage of extremely high safety.

  Since antibacterial products that demand the construction of platinum and other metals alone are commercially available, platinum particles alone or the metal particles alone were considered to have a certain antibacterial effect, but the present invention was completed. In the tests conducted in the process, each metal particle alone had no effect. In contrast, by including both the platinum particles and the metal particles as in the microbial control agent and the acaricide composition of the present embodiment, excellent microbial control performance that cannot be achieved with each metal particle alone is achieved. And synergistically improves antibacterial performance.

  Furthermore, since the microbial control agent and the acaricide composition are configured to contain platinum particles and the metal particles, there is a very low possibility that conventional metal ions will be eluted. Therefore, the microbial control agent and the acaricide composition can also reduce the influence on the environment.

  According to the microorganism control agent of the present embodiment, since it has excellent antibacterial performance and metal elution is also reduced, it is easy to impart a microorganism control function to an object, and as a method for controlling microorganisms Suitable for use. In addition, the use of the above-mentioned microorganism control agent is very useful because the processing effort can be halved and the processing cost can be reduced. Therefore, the above microorganism control agent is used for industrial purposes (for example, hydrous pulp, coated paper, paper coating liquid, paint, binder, adhesive, latex, ink, etchant, soaking water, wood, fiber, wood In addition to flour, plastics, cement admixtures, sealing agents, resin emulsions, building materials, etc., many industrial products or industrial raw materials), especially agricultural fields such as planting where safety is a priority, foods such as antibacterial bottles, etc. It can be applied to various fields such as fields, physics and chemistry materials such as cell culture substrates, and is particularly useful as an antibacterial agent, an algaeproofing agent, and a biofilm formation inhibitor.

  Moreover, according to the acaricide composition of this embodiment, this can be applied to a target object to form an acaricidal member. Such a tick-proof member is formed with platinum particles and metal particles other than platinum contained in the mite-killing composition, and therefore has excellent performance in suppressing the occurrence and increase of mites, so Can be used for all materials that require ticks, for example, suitable for life-related materials such as tatami, carpets, wall materials, flooring materials, ceiling materials, furniture, household appliances, food containers, food packaging materials, kitchen products, etc. For example, as an industrial material, it is suitable as a member for growing or storing plants, animals, insects, etc. (excluding beekeeping members).

  EXAMPLES Hereinafter, although an Example demonstrates this invention more concretely, this invention is not limited to the aspect of these Examples.

1. Confirmation of Antibacterial Effect (Preparation Example 1) Preparation of Platinum Particle Dispersion Solution 0.212 g of potassium chloroplatinate (K ₂ PtCl ₄ ) was dissolved in 50 mL of pure water to prepare a potassium chloroplatinate solution. Separately, 0.129 g of sodium citrate was dissolved in 50 mL of pure water to dissolve a 0.01 mol / L sodium citrate aqueous solution, and 0.881 g of ascorbic acid was dissolved in 50 mL of pure water to give 0.1 mol. / L ascorbic acid aqueous solution was prepared respectively. After adding 50 mL of the above potassium chloroplatinate aqueous solution to 850 mL of pure water, 50 mL of 0.01 mol / L sodium citrate aqueous solution and 50 mL of 0.1 mol / L ascorbic acid aqueous solution were added, respectively, and stirred at 100 rpm for about 5 minutes. And reacted. Next, ion exchange membrane treatment was performed to remove ionic impurities in the platinum particle dispersion, and a colloidal solution containing platinum particles was obtained. The particle diameter of the platinum particles measured with a zeta potential measuring device (Zeta Sizer Nano ZS90, manufactured by Malvern) was 130 nm. Moreover, the purity of platinum in the platinum particles measured by TEM / EDS (HITACHI H-7100, acceleration voltage 100 kV) was 100%.

  The platinum concentration of the dispersion obtained by the above method was confirmed by ICP-MS (ElanDRCII manufactured by Perkin Elmer) and further diluted with water to obtain a dispersion (dispersion 1) having a platinum particle concentration of 100 mg / L. It was.

Preparation Example 2 Preparation of Silver Particle Dispersion Solution 0.157 g of silver nitrate (AgNO ₃ ) was dissolved in 50 mL of pure water to prepare a silver nitrate aqueous solution. Separately, 10 wt% ammonia water and 0.881 g of ascorbic acid were dissolved in 50 mL of pure water to prepare 0.1 mol / L ascorbic acid aqueous solutions. 50 mL of the above 0.01 mol / L silver nitrate aqueous solution was added to 900 mL of pure water, and then adjusted to pH 11 with 10 wt% ammonia water. Furthermore, 0.1 mol / L and 1 mL of ascorbic acid aqueous solution were added, and it was made to react by stirring at 100 rpm for about 5 minutes. Next, an ion exchange membrane treatment was performed to remove ionic impurities in the silver particle dispersion, thereby obtaining a colloidal solution containing silver particles. The particle diameter measured by a zeta potential measuring device (Zeta Sizer Nano ZS90, manufactured by Malvern) was 130 nm. Further, the purity of silver in the silver particles measured by TEM / EDS (HITACHI H-7100, acceleration voltage 100 kV) was 100%.

  The silver concentration of the dispersion obtained by the above method is confirmed by ICP-MS (ElanDRCII manufactured by Perkin Elmer), and further diluted with water to obtain a dispersion (dispersion 2) having a silver particle concentration of 100 mg / L. It was.

(Preparation Examples 3 to 7)
By mixing the dispersion liquid 1 and the dispersion liquid 2 obtained in Preparation Example 1 and Preparation Example 2 at the blending ratios shown in Table 1, respectively, a mixed liquid of platinum particles and silver particles for use in Examples described later. (Preparation Examples 3 to 7, respectively) were obtained.

(Preparation Example 8)
Dispersion liquid 1 (platinum particle dispersion liquid) obtained in Preparation Example 1 and dispersion liquid 3 in which nano copper (manufactured by Sigma, catalog number 774111, particle diameter 40-60 nm, 99%) is dispersed in water The mixture was quantitatively mixed to prepare a mixed solution in which the mixing ratio of platinum particles and copper particles was 70/30 by mass ratio and the total concentration of both metal particles was 100 mg / L.

(Preparation Example 9)
A predetermined amount of the dispersion 1 (platinum particle dispersion) obtained in Preparation Example 1 and a dispersion 4 in which nickel particles (manufactured by Sigma, catalog part number 577995, particle size <100 nm, 99%) are dispersed in water The mixture was mixed to prepare a mixed solution in which the mixing ratio of platinum particles and nickel particles was 70/30 in mass ratio, and the total concentration of both metal particles was 100 mg / L.

(Preparation Example 10)
A predetermined amount of the dispersion 1 (platinum particle dispersion) obtained in Preparation Example 1 and zinc oxide particles (manufactured by Sigma, catalog part number 721085, particle size <130 nm, 40%) as the dispersion 5 were mixed to obtain platinum particles. And a mixture of zinc oxide particles in a mass ratio of 70/30 and a total concentration of both metal particles of 100 mg / L.

  Table 2 shows the compositions of Preparation Examples 8 to 10.

Example 1
8 parts by mass of a base material made of polytetrafluoroethylene resin (PTFE resin) (2 × 3 cm net shape and 1 mm square opening), washed with pure water, and mixed in Preparation Example 3 above It was immersed in the liquid. After that, the mixed solution in which the base material was immersed was placed in a test microwave device (Multiwave 3000 manufactured by PerkinElmer) and subjected to microwave irradiation treatment. Microwave irradiation conditions were as follows: irradiation wavelength 2.45 GHz, irradiation power 400 W, irradiation time 7 min. Thereafter, the base material was pulled up, washed with pure water, and dried overnight at room temperature to obtain a microorganism control agent.

(Examples 2 to 8)
A microorganism control agent was obtained in the same manner as in Example 1 except that the mixed solution prepared in Preparation Example 3 was replaced with the mixed solution prepared in Preparation Examples 4 to 10 (Examples 2 to 8 respectively).

(Comparative Example 1)
A microorganism control agent was obtained in the same manner as in Example 1, except that the mixed solution prepared in Preparation Example 3 was replaced with the dispersion 1 obtained in Preparation Example 1.

(Comparative Example 2)
A microorganism control agent was obtained in the same manner as in Example 1 except that the mixed liquid prepared in Preparation Example 3 was replaced with the dispersion 2 obtained in Preparation Example 2.

(Antimicrobial evaluation)
The test specimens obtained in each Example and Comparative Example were subjected to an antibacterial test by a film adhesion method according to a measurement method based on JIS Z2801. Moreover, as a microbial cell, it tested using each of yellow staphylococcus and colon_bacillus | E._coli. The results are shown in Table 3.

Example 9
A microbial control agent in which platinum particles and silver particles were supported on a base material was obtained in the same procedure as in Example 2 except that the base material was made of polycarbonate (PC resin) instead of the base material made of PTFE resin. .

(Example 10)
A microorganism control agent was obtained in the same procedure as in Example 2 except that the mixture obtained in Preparation Example 4 was diluted to 1/10 with water and used.

(Example 11)
A microorganism control agent was obtained in the same procedure as in Example 10 except that the base material was made of polycarbonate (PC resin) instead of the base material made of PTFE resin.

(Example 12)
As a microwave irradiation condition, a microorganism control agent was obtained in the same procedure as in Example 2, except that the treatment with an irradiation wavelength of 2.45 GHz, an irradiation power of 400 W, and an irradiation time of 7 min was performed three times instead of once.

(Example 13)
A microorganism control agent was obtained in the same procedure as in Example 12 except that the substrate was made of polycarbonate (PC resin) instead of the PTFE resin substrate.

(Example 14)
Change to a polystyrene (PS resin) base material instead of a PTFE resin base material, and as a microwave irradiation condition, treatment with an irradiation wavelength of 2.45 GHz, an irradiation power of 400 W, and an irradiation time of 7 min at a time Instead, the microorganism control agent was obtained in the same procedure as in Example 2, except that the procedure was performed 5 times.

  In Examples 1 to 5 and Examples 9 to 14, the platinum / silver fine particle mixed solution before and after microwave irradiation was sampled, and a silver ion measuring instrument (AGT-131, manufactured by Nippon Ion Co., Ltd.) was used. When the silver ion concentration was confirmed, all were below the detection limit.

<Evaluation method>
(Measurement of metal loading)
About the microorganism control agent produced by said each Example and comparative example, the metal carrying amount was measured according to the sulfuric acid ashing method. Specifically, (1) sulfuric acid is added to the sample, carbonization and ashing are carried out, (2) aqua regia dissolution, (3) after ashing, aqua regia is added and dissolution treatment is carried out, (4 ) The amount of metal in the resulting solution was measured using ElanDRCII manufactured by PerkinElmer.

(Measurement of metal elution amount)
Add 10 mL of ion-exchanged water and the microorganism control agent prepared in Example 2 to a 100 mL Erlenmeyer flask, place in a constant temperature shaker at 35 ° C., and shake for 24 hours under conditions of an amplitude of 30 mm and a horizontal shake of 150 rpm. did. The amount of metal in the obtained immersion liquid was measured by IPC-MS (ElanDRCII manufactured by Perkin Elmer Co.), thereby quantifying the amount of metal elution from the substrate.

(Measurement of antibacterial activity value)
According to the measurement method based on JIS Z2801, the antibacterial property test was done by each of the film adhesion method and the shake method. Moreover, as a microbial cell, it tested using each of yellow staphylococcus and colon_bacillus | E._coli.

  Prior to measuring the antibacterial activity value of the microbial control agent obtained in each Example, the antibacterial activity test of the dispersion obtained in Preparation Example 4 was carried out. showed that. Considering this result, it can be seen that the dispersion itself containing platinum particles and silver particles also has an excellent antibacterial action.

  Table 4 shows the amount of metal (platinum and silver) supported on the base material in the microbial control agents produced in each Example and Comparative Example. In any of the examples, it can be seen that platinum and silver are supported in a certain amount on the substrate.

  Table 5 shows the measurement results of the metal elution amount from the microorganism control agent prepared in Example 2. As a result, the amount of metal elution from the single substance of the microorganism control agent of Example 2 was below the detection limit. Nevertheless, as described later, the microorganism control agent of Example 2 exhibits an excellent antibacterial effect.

  Table 6 shows the antibacterial activity value against bacterial cells (yellow staphylococci and Escherichia coli) in the film adhesion method. Although the base material made of PC resin (Examples 9, 11, and 13) had a smaller metal loading than the base material made of PTFE resin (Example), the same excellent antibacterial performance was exhibited.

2. Confirmation of anti-algae effect (Example 15)
Plant cultivation container (As One Violamo polycarbonate square bottle, material: polycarbonate, capacity: 150 mL) After washing the interior with pure water, the mixture (platinum / silver particle dispersion) prepared in Preparation Example 4 is 100 times with pure water. Diluent was added. Then, it installed in the test microwave apparatus (Multiwave3000 by PerkinElmer), and performed the microwave irradiation process. Microwave irradiation conditions were as follows: irradiation wavelength 2.45 GHz, irradiation power 400 W, irradiation time 7 min. The platinum / silver particle dispersion in the container was removed, the interior of the container was washed with pure water, and then dried overnight at room temperature to obtain a plant cultivation container having platinum particles and silver particles supported on the inner surface.

(Example 16)
As the microwave irradiation conditions, platinum particles and silver particles were formed on the inner surface in the same procedure as in Example 15 except that the treatment with an irradiation wavelength of 2.45 GHz, an irradiation power of 400 W, and an irradiation time of 7 min was performed twice instead of once. A plant cultivation container on which was supported was obtained.

(Comparative Example 3)
A plant cultivation container having platinum particles supported on the inner surface was obtained in the same procedure as in Example 15 except that the dispersion was changed to Dispersion 1 (Preparation Example 1) containing only platinum particles but not silver particles. .

(Comparative Example 4)
A plant cultivation container having silver particles supported on the inner surface was obtained in the same procedure as in Example 15 except that the dispersion was changed to Dispersion 2 (Preparation Example 2) containing only silver particles but not platinum particles. .

(Algae prevention test)
The plant culture solution (100-fold diluted solution of OAT House No. 8 manufactured by OAT Agrio) was put into the containers prepared in Examples 15 to 16 and Comparative Examples 3 to 4, where cucumber seedlings were cultivated for 8 days, The occurrence of algae was confirmed visually.

  Table 7 shows the results of the algae control results.

  From Table 7, there was no generation of algae in Examples 15 and 16, and from this result, it was confirmed that the microbial control agent in which platinum and silver particles were supported on the base material exhibited an algal control effect.

3. Confirmation of biofilm formation inhibitory effect (Example 17)
The microorganism control agent (PTFE base material carrying platinum and silver particles) produced in Example 2 was cut into 5 cm × 5 cm to obtain test pieces.

(Example 18)
A microorganism control agent in which platinum and silver particles are supported on a substrate in the same manner as in Example 2, except that a platinum-silver particle dispersion obtained by diluting the mixed solution prepared in Preparation Example 4 with pure water 10 times was used. (PTFE base material carrying platinum and silver particles) was prepared and cut into 5 cm × 5 cm to obtain a test piece.

(Example 19)
A microorganism control agent (platinum and platinum) in which platinum and silver particles are supported on a substrate in the same manner as in Example 2 except that the mixed solution prepared in Preparation Example 4 was diluted 100 times with pure water. A PTFE base material carrying silver particles was prepared, and this was cut into 5 cm × 5 cm to obtain test pieces.

(Comparative Example 5)
Neither platinum nor metal particles were supported, and a PTFE base material was cut into 5 cm × 5 cm to obtain a test piece.

(Confirmation test of biofilm suppression effect)
10 L of tap water was put into a constant temperature water tank of a constant temperature water tank with an external circulation device, and self-circulated without being circulated to an external circulation target. The test piece of any of Examples 17 to 19 and Comparative Example 5 is immersed in this constant temperature water bath, and 8 hours from 9:00 to 17:00 every day is set as the operating time, and the operating temperature is in the range from room temperature to 40 ° C. I drove for 10 days. Thereafter, the formation of a biofilm (film-like substance) on the surface of each test piece was visually evaluated.

  Table 8 shows a confirmation test of the biofilm suppression effect.

  From Table 8, in Examples 17-19, there was no formation of a biofilm, and from this result, it was confirmed that a biofilm formation inhibitory effect was manifested in the microbial control agent in which platinum and silver particles were supported on a substrate. It was done.

4). Confirmation of mite killing and mite prevention effect (1)
(Example 20)
The test was conducted according to JIS L 1920: 2007. Specifically, a processed or unprocessed sample is spread on a sample bottle, 50 to 80 animals / 0.1 g of mite medium (Yake leopard mite) is dispersed in each sample, and then left for 4, 6 and 8 weeks, By counting the number of surviving ticks in the tube bottle, the growth inhibition rate of ticks was calculated. The processed sample was prepared by treating PE resin pellets (Φ10 mm × L10 mm) as a substrate with an aqueous dispersion (No. 1 to 4) of the acaricide composition having the composition ratio shown in Table 9. All samples were prepared by microwave irradiation (output 400 W, time 14 min).

Then, the mite repellent rate was calculated by the following formula.
Repelling rate (%) = [(A−B) / A] × 100
Here, A represents the number of attracted ticks of the unprocessed sample, and B represents the number of attracted ticks of the processed sample.

  Table 10 shows the No. shown in Table 9. 1-No. The repelling rate in the processed sample processed with the sample of 4 is shown.

  From this result, it was confirmed that an excellent mite-preventing effect was exhibited in the mite-proof member in which platinum and silver particles were supported on the base material.

5. Confirmation of mite killing and mite prevention effect (2)
(Reference Test Example 1)
A PLA (polylactic acid resin) honeycomb (manufactured by Kunimune Co., Ltd.) was used. By immersing in 1 (mixed dispersion of platinum particles and silver particles), a PLA honeycomb carrying platinum particles and silver particles was obtained as a spleen-proof spleen.

  The obtained tick-proof honeycomb was placed in an acrylic cage (width 40 cm, depth 10 cm, height 20 cm), and five bee wings were placed in an incubator (manufactured by Panasonic) at 30 ° C. and 70% humidity. The animals were reared after adjusting to the conditions. The survival rate of the honeybee mite was measured 2 hours, 4 hours, 6 hours and 24 hours after the breeding. This operation was repeated 6 times to calculate the average survival rate.

  FIG. 1 shows the relationship between rearing time and the number of living honey bee mites. For comparison, the results of tests similar to those for anti-tick nest spleens were also applied to untreated PLA honeycombs, that is, honeycombs not carrying platinum particles and silver particles (hereinafter referred to as “blank nest spleens”). (In each elapsed time of FIG. 1 bar graph, the left bar graph shows the number of surviving ticks of the mite-preventing spleen, and the right bar graph shows the number of surviving ticks of the blank nest spleen).

  From this result, it can be seen that the number of honey bee mite ticks decreased with the passage of breeding time in the tick-proof nest spleen compared with the blank nest spleen. Therefore, it can be said that the honeycomb treated with the acaricide composition containing platinum particles and silver particles has an acaricidal effect and an acaricidal effect.

6). Confirmation of mite killing and mite prevention effect (3)
(Reference Test Example 2)
The mite-preventing spleen obtained in the confirmation of the mite-killing and mite-preventing effect (2) and the blank nest spleen are placed in an acrylic cage (width 40 cm, depth 10 cm, height 20 cm). The animals were housed and reared in an incubator (manufactured by Panasonic) under conditions of 30 ° C. and 70% humidity. After 2 hours, 4 hours, 6 hours, and 24 hours after breeding, it was confirmed in which nest spleen the honeybee mite was present. This operation was repeated 6 times to calculate the average number of honey bee mites in each nest spleen.

  FIG. 2 shows the relationship between breeding time and the number of honey bee mite survivals (the left bar graph shows the number of mite-preventing spleen survival ticks and the right bar graph shows blank nest spleen survival at each elapsed time in FIG. 2). Shows the number of ticks).

  From this result, it can be seen that, as the breeding time elapses, the number of honey bee mite survivors in the mite-preventing spleen is greatly reduced compared to the blank nest spleen. Therefore, it can be said that the honeycomb treated with the acaricide composition containing platinum particles and silver particles has a repellent effect against mites.

7). Confirmation of cell culture (Example 21)
After washing a polystyrene resin (PS resin) dish (tissue culture dish “FALCON3001”, diameter 35 mm), which is a cell culture container, with pure water, the sample No. shown in Table 9 was obtained. The dish was subjected to antibacterial treatment by being immersed in a dispersion containing platinum particles and silver particles. Thereafter, the mixed solution in which the dish was immersed was placed in a test microwave device (Multiwave 3000 manufactured by PerkinElmer) and subjected to microwave irradiation treatment. Microwave irradiation conditions were as follows: irradiation wavelength 2.45 GHz, irradiation power 400 W, irradiation time 7 min. Thereafter, the dish was pulled up, further immersed in 70% EtOH for several minutes, the dish was pulled up, and then sterilized by irradiation with ultraviolet rays for about 1 hour. Thereby, an antimicrobially treated cell culture container was obtained.

Using the cell culture vessel, cells were cultured under the following conditions.
Cell conditions-HepG2 cells (human hepatoblastoma cells)
-Medium: WE + 10% FBS
・ Culture period: 5 days (without medium change)
Cell seeding density 1.5 × 10 ⁵ cells / dish
(1.6 × 10 ⁴ cells / cm ² )
Culture conditions-Volume of culture solution: 2 ml / dish (35 mm)
Culture: 37 ° C., 5% CO ₂ atmosphere.

(Example 22)
A dispersion containing platinum particles and silver particles was prepared as Sample No. A cell culture vessel was obtained under the same conditions as in Example 21 except that the cells were changed to 1, and cells were cultured.

(Blank test)
Cells were cultured under the same conditions as in Example 21 except that the antibacterial treatment with the dispersion containing platinum particles and silver particles was not performed.

  Table 11 shows the amounts of platinum and silver supported on the cell culture container, and also shows the antibacterial results and the value of albumin secretion activity.

  The supported amounts of platinum and silver were quantified using ICP-MS after dissolving the dish to obtain a metal-containing liquid. The supported amounts of platinum and silver were measured in the same manner as the above-mentioned “Measurement of metal supported amount”.

  The value of albumin secretion activity is an index indicating the activity of cells. The value of albumin secretion activity was measured by the following method. First, the amount of albumin secreted into the culture medium was quantified by enzyme-labeled immunoassay (ELISA), and the albumin secretion rate per unit cell number was converted from this quantified value. The number of cells was calculated by DNA-DAPI (4,6-diaminodino-2-phenylindole, manufactured by Wako Pure Chemical Industries, Ltd.) fluorescence method. That is, a calibration curve between DNA extracted from certain cells and the fluorescence intensity of DNA-DAPI was prepared, and the number of cells cultured was calculated based on this relationship. Based on the number of cells, the albumin secretion rate per unit cell was calculated.

The antibacterial evaluation was judged according to the following criteria from the antibacterial activity value by the shake method (35 ° C. ± 1 ° C.) according to the measurement method in accordance with JIS Z2801. E. coli was used as the microbial cell.
◯: The antibacterial activity value was 2.0 or more and had excellent antibacterial properties.
X: The antibacterial activity value was less than 2.0, and it did not have antibacterial properties.

  From Table 11, since the cell culture container of Examples 21-22 has high antibacterial property, since the activity of a cell is equivalent to a blank test, antibacterial treatment does not affect the culture of a cell. I understand that.

  Furthermore, using the cell culture vessel obtained in Example 22, NIH3T3 cells (mouse fibroblasts), HeLa cells (human cervical cancer cells), and hMSCs (human mesenchymal stem cells) were cultured under the same conditions. However, no influence on the culture of these cells was observed (it was confirmed that these cells were cultured without problems).

Claims (14)

Including the micro-biological control agent, a Bomozai,
The microorganism control agent includes platinum particles and at least one metal particle selected from the group consisting of silver particles, copper particles, nickel particles and zinc particles,
An anti-algae agent wherein the platinum particles are 10 or more and 90 or less when the total mass of the platinum particles and the metal particles is 100 . The algae deterrent according to claim 1, wherein the metal particles include at least one selected from the group consisting of silver particles and copper particles. The anti-algae agent according to claim 1 or 2, wherein the platinum particles and the metal particles have an average particle diameter of 0.1 to 1000 nm. The anti-algae agent of any one of Claims 1-3 with which the said platinum particle and the said metal particle are carry | supported on the base material. The platinum particles and the metal particles are 0.01 to 20,000 ng / cm. ² The algae-proofing agent according to claim 4, which is carried on the substrate. The anti-algae agent of Claim 4 or 5 in which the said base material is formed with resin. The algae according to claim 6, wherein the resin includes at least one selected from the group consisting of polystyrene resin, vinyl chloride resin, fluorine resin, ABS resin, PET resin, polycarbonate resin, urea resin, olefin resin, and phenol resin. Agent. The base material according to any one of claims 4 to 7, wherein the base material is formed into any one of a sheet shape, a plate shape, a block shape, a net shape, a punching sheet shape, a granular shape, a rod shape, a crushed shape, and a dish shape. The algae preventer according to item 1. Thereby imparting antialgal the object using microbial control agent, a algae method,
The microorganism control agent includes platinum particles and at least one metal particle selected from the group consisting of silver particles, copper particles, nickel particles and zinc particles,
An anti-algae method wherein the platinum particles are 10 or more and 90 or less when the total mass of the platinum particles and the metal particles is 100. The algae prevention method according to claim 9, wherein the platinum particles and the metal particles are supported on a base material. Comprising platinum particles, and silver particles child, when the total mass of platinum particles and the silver particles 100, the platinum particles is 10 or more and 90 or less, acaricidal composition. The acaricidal composition according to claim 11, wherein the platinum particles and the silver particles have an average particle diameter of 0.1 to 1000 nm. A tick-proof member (excluding a beekeeping member) using the acaricidal composition according to claim 11 or 12 . The mite prevention method which provides a target object (however, except a beekeeping member) the mite prevention function using the acaricide composition of Claim 11 or 12 .

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