JPH11221025A - Solid water for replenishing water to animal and feeding the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a means of feeding replenishing water, with standing the vibration of transportation such as a transport vehicle, enabling water to be safely replenished to experimental animals or pets, and also enabling water to be easily and safely fed to pets or hunting dogs such as field trial dogs under the circumstance of no means of replenishing water within reach. SOLUTION: This solid water for animals comprises water and a gelling agent, being not in the form of solution but holding solidity, therefore with standing the vibration of transportation such as a transport vehicle and affording a gelled water replenisher with its solidity retained. This solid water can be willingly taken by animals when they are in transportation within a closed space or there is not water replenishing spot within reach, leading to preventing the animals from stress and maintaining their healthy conditions, Intake of this solid water is high with its slight variance throughout the time period of transportation when it has a jelly strength of 50-1,000 g/cm<2> .

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to hydration solid water for feeding to animals, and more particularly to hydration when transporting experimental animals and hydration when transporting pets (pets). And solid water for simple hydration during use of a hunting dog such as a field trial dog or during field trial training.

[0002]

2. Description of the Related Art Laboratory animals use GLP (Good Laboratory Pr
actice) and pharmacodynamic (Non GLP) tests. Monkeys, dogs, cats, rats, mice, guinea pigs or ferrets are known as the experimental animals,
Taking an experimental dog as an example, in general, dogs aged 5 to 6 months are subjected to the above test (GLP test /
(Non-GLP test), for example, to a drug safety laboratory of a pharmaceutical company, a central research laboratory, a contract laboratory, a university, a laboratory of a government agency, or the like. When transporting,
The dog should be kept in isolation from the outside world so that the dog is not susceptible to various infections and external sensitization during transportation, and should be fasted the day before the day of transportation and should only be hydrated during transportation. Is commonly done. There are various methods for hydration, and these methods supply drinking water as a liquid.

[0003]

The time required for transporting the experimental animals is often relatively long. If the experimental animals being transported are not replenished with feed and water, the animals are subjected to strong stress. At the time of arrival, there are cases where it is weakened by dehydration. Therefore, it is desirable to supply only a necessary amount of water during transportation, as described above. However, the current hydration method is a method of supplying water from an automatic water supply device provided in a cage for holding laboratory animals.

[0004] However, the vibration of the transport vehicle (with suspension) sometimes causes water to spill on the floor when water is supplied from the automatic water supply device, and the environment inside the transport vehicle may be deteriorated. Also,
Due to the material, form and nature of the automatic waterer, the animals may be injured by the vibration of the transport vehicle.

[0005] As described above, there are some points to be improved in the drinking method using the automatic water supply device provided in the cage for holding the experimental animal in the transport vehicle. Therefore, there is no best way to rehydrate experimental animals being transported by transport vehicle. At present, experimental animals such as beagle dogs may be weakened when they arrive at their destination.

Also, due to the recent pet boom, dogs, cats,
The breeding of small birds, ornamental fish, and other pets (pets) is thriving, but during the transportation of living organisms from breeding grounds to sales bases (retail stores such as pet shops), and from the sales bases to real breeders' homes ( The pet is subjected to the same stress as in the case of an experimental animal due to being trapped in the transport vehicle for a long time, for example, during transportation to a pet owner's house. In addition, pets are often accompanied by real breeders (pet owners) and go on outdoor trips using camper cars. For example, pets are kept in vehicles for a long time, The same stress is applied as in the case of experimental animals.

Further, in the hunting season, if a hunting dog is not in the water near the hunting ground while using the hunting ground, it is difficult to supply water, and there is a need for a method for easily supplying water.
There is also a demand for not only hunting dogs but also hydration during field trial training.

[0008] Against this background of the problems of the prior art, an object of the present invention is to withstand the vibration of a transportation means such as a transportation vehicle and to safely hydrate a laboratory animal or a pet animal. It is to provide make-up water dispensing means and to easily and safely supply water to pets or hunting dogs in situations where there is no means of hydration at hand.

[0009]

Means for Solving the Problems In order to solve the above problems, the present inventors have developed solid water for hydration of animals containing water and a gelling agent. The solid water of the present invention is solid water for hydration of an animal containing water and a gelling agent, and the solid water for hydration is used when transporting an experimental animal in a closed vehicle, transporting a companion animal, or performing a field trial. It can be provided for hydration during use of a hunting dog such as a dog or during field trial training.

Since the solid water of the present invention is not a solution but has a solid property, it is a gelled water replenishing agent which does not break the solid property by withstanding the vibration of a transportation means such as a transport vehicle. The solid water of the present invention can be favorably consumed by hunting dogs such as experimental animals, companion animals, and field trial dogs when transported in an enclosed space or when there is no place for hydration nearby. As the raw material water used for the solid water of the present invention, tap water is sufficient, but water with higher purity may be used.

Gelling agents used for solidifying water include those having a gel-forming ability, such as carrageenan, gelatin, pectin, tamarind sea gum, alginate, and galactomannan, and complexes thereof. To which guar gum, locust bean gum, tara gum and other thickening polysaccharides are added.

Phosphates, saccharides, various metal salts and the like may be added to enhance the dispersibility and water solubility of the components of the gelling agent. In addition, gelation is performed according to the taste of hunting dogs such as experimental animals, companion animals and field trial dogs, and the blood biochemical properties of animals receiving solid water (affected by the degree of dehydration and stress). It is possible to adjust the concentration of the agent component so as to have an appropriate hardness (jelly strength).

Further, in order to enhance the palatability of the obtained solid water, all additives having a palatability enhancing effect such as various flavors and various extracts can be used. Similarly to the gelling agent concentration, it is possible to adjust the blending amount in accordance with the taste of the target animal group and the blood biochemical property value of the animal being fed. It is also possible to add functional substances typified by oligosaccharides, catechins or dietary fiber, various vitamins, minerals or various pharmaceuticals to the solid water.

The amount of the jelly strength and the palatability enhancer affects the amount of intake and the state of intake (change in amount of intake per hour / degree of recovery of dehydrated state, etc.). Therefore, it is necessary to adjust according to the attributes of the experimental animal (such as a pet animal or an experimental animal) and the feeding time (how long it takes to ingest).

Before transporting an animal to be tested by a transport vehicle so as not to cause vomiting during transport, the animal is in a fasted state. However, when fasting animals rapidly ingest fluid,
Undesirably, it causes diarrhea and vomiting. Also, if the animal consumes almost all of the supplied solid water shortly after feeding, it may not be possible to take in water in the second half of the long transport period. From this, it is most preferable that after the animal ingests a certain amount of water immediately after feeding, the intake gradually decreases and thereafter becomes a stable intake, and there is little variation in the intake over the entire transportation time zone, and Those with a high intake are preferred.

As a result of intensive studies, the present inventors have found that the jelly strength of solid water is a factor for selecting solid water having a relatively small intake and a relatively high intake over the entire transportation time zone. Was found. That is, jelly strength are those appropriate to the to feed less animal variation throughout the 50 to 1000 g / cm ² about a solid water transportation time period, when the jelly strength is less than 50 g / cm ^2, in the vehicle If the shape retention during transportation cannot be maintained, and if the jelly strength exceeds 1000 g / cm ² , the palatability of the animal will be extremely reduced. In particular, jelly strength 100
４５０450 g / cm ² , more preferably 200 to 300 g
It has been found that the solid water in the range of / cm ² has a small variation in the intake of the animal over the entire transportation time zone and a high intake.

When the kind of the gelling agent or the mixing ratio of a plurality of gelling agents is changed, the concentration of the gelling agent greatly changes. Therefore, the gelling agent concentration cannot be used as a general index that does not vary in the intake amount over the entire transportation time regardless of the type of the gelling agent, the mixing ratio of the gelling agent, and the like. However, the jelly strength does not change as much as the gelling agent concentration even if the type of gelling agent, the mixing ratio of the gelling agent to be mixed, etc. change variously. It can be used as an index for obtaining. Further, the amount of the palatability enhancer varies depending on the palatability attraction of the attractant used, but is suitably about 0.01 to 5% by weight.

The solid water of the present invention can withstand the vibration of a vehicle, maintain its shape-retaining property, and can safely and sufficiently supply water to experimental animals during transportation which have been confined in an enclosed space for a long time. The hemoglobin value and hematocrit value when the experimental animal that ingested the solid water of the present invention arrived at the destination did not increase, so the solid water of the present invention prevented the stress of the animal and improved the health condition. It has been found that it has helped to maintain it sufficiently.

The solid water for hydration to be supplied to the animal of the present invention is not limited to simple hydration for transportation of experimental animals,
It can also be used as solid water for simple hydration during transportation of pets (pets) and during hunting dogs such as field trial dogs or for simple hydration during field trial training. It promotes metabolism in the body and is effective in maintaining health.

[0020]

Embodiments of the present invention will be described. The solid water that is a gelled water replenisher is produced as follows. That is, after a predetermined amount of a carrageenan formulation as a gelling agent is mixed while stirring tap water, the mixture is heated and stirred to completely dissolve the gelling agent.
The melt is filled into a plastic film (vinylidene chloride film) bag, ligated, and the resulting sausage-type filling is sterilized at 100 ° C. for 50 minutes. Cool until solidified to obtain a solid water product.

Selection of the optimum concentration of the gelling agent in the solid water produced using a carrageenan preparation (Ace Agar AG-103C (Miyagi Chemical Industry Co., Ltd.)) as the gelling agent, and confirmation of the taste and intake of the solid water. The next test for
~ Test 7. Was done.

Test 1. Test Method Fasted and water-fasted animals (beagle dogs) were divided into three groups each consisting of 10 animals, to which 0.5%, 1.5% and 3.0% gelling agents were given, respectively, for 4 hours. Intake and body weight measurements were taken after 8 and 24 hours.

Table 1 shows the results of the intake, blood biochemical properties and the average weight of each group. Hereinafter, the solid water intake, the average value thereof, and the body weight of the animals shown in each table including Table 1 are all shown in gram units. In addition, RB in each table
C, represents WBC, Hb, respectively Ht erythrocytes (× 10 ⁶ cells / 100 ml), white blood cells (× 10 ³ / 100ml), hemoglobin (× g / 100 ml), the measurement of hematocrit (%). When the WBC value rises from the normal value, it is considered that trauma or inflammation has occurred in the body, and when the values of Hb and Ht rise, dehydration appears and it is estimated that there is stress.

[0024]

[Table 1]

As shown in Table 1, the palatability was evaluated for 0 to 4 hours, 4 to 8 hours, depending on the gelling agent concentration in the solid water.
The test was performed for 8-24 hours and 0-24 hours of intake. As shown in Table 1, solid water having a gelling agent concentration of 0.5%
g, and the intake in each of the above times was 67.4.
g, 7.0 g, 9.5 g, and 83.9 g. Also,
When 94 g of solid water having a 1.5% gelling agent concentration was given, the intake amount during each time was 41.2, 5.0, 5.0, 5
1.2 g. In addition, when 93 g of solid water having a gelling agent concentration of 3.0% was given, the intake amount during each time was 14.7.
g, 1.7 g, 16.1 g, and 33.5 g.

According to the results, if the gelling agent concentration (in this case, there is a certain correlation between the gelling agent concentration and the jelly strength because it is solid water composed of the same gelling agent component), it is ingested if it is too high. It was found that the amount of intake was greatest when the amount was reduced and when the amount of solid water was 0.5% gelling agent. Although a 0.5% gelling agent concentration is considered to be an appropriate concentration, the jelly strength is weak and the shape retention cannot be maintained, and there is a possibility of spilling.

Further, the above test 1. As for the amount of gelling agent given in 24 hours, 4 individuals at 0.5% gelling agent concentration and 4 cases at 1.5% gelling agent concentration showed that The amount was also examined next.

Test 2. Fasted and water-fasted animals (beagle dogs) were divided into 5 groups, each group consisting of 7 animals, and a carrageenan preparation (Ace Agar AG-10) was used as a control and gelling agent.
0.7%, 0.3% by using 3C (Miyagi Chemical Industry Co., Ltd.).
Give solid water at 9%, 1.1% and 1.3% gelling agent concentration respectively, intake 0-4 hours, 4-8 hours, 8-24 hours and 0-24 hours intake, blood tests and Body weight was measured. Table 2 shows the results of the intake of each group and the average value of body weight measurement.

[0029]

[Table 2]

When 189 g of solid water having a gelling agent concentration of 0.7% was given, the intake amount at each time was 45 g, 5 g,
8 g, 58 g of solid water 1 having a gelling agent concentration of 0.9%
When 90g is given, the intake amount at each time is 41g, 3g,
19 g and 63 g. Further, when 188 g of solid water having a gelling agent concentration of 1.1% was given, the amount of intake per hour was 30.
g, 28 g, 38 g, and 97 g. When 194 g of solid water having a 1.3% gelling agent concentration was given, the intake amounts at each hour were 29 g, 15 g, 71 g, and 114 g.

That is, the solid water at the concentration of 0.7% and 0.9% of the gelling agent has an intake of 0 to 4 hours that is approximately 70% of the intake of 0 to 24 hours, and a 1.1% gelation. In the case of solid water having the agent concentration, the intake amount for 0 to 4 hours, 4 to 8 hours, and 8 to 24 hours was almost 30% of the intake amount for 0 to 24 hours.
The 1.3% gelling agent concentration of the solid water was such that the intake for 8 to 24 hours was 60% of the intake for 0 to 24 hours.

The solid water at 0.7% and 0.9% gelling agent concentration is ingested most of the time within 4 hours of feeding, and 1.1%
Solid water with a% gelling agent concentration was taken almost on average at each time zone, and solid water with a 1.3% gelling agent concentration tended to be taken after 8 hours. Therefore, the optimum concentration of the gelling agent (thickening agent) represented by the carrageenan preparation in solid water is 1.1.
%Met.

The amount of the gelling agent in the solid water is as follows:
Individuals who ingested all of the supplied amount had 0.7% gelling agent concentration and 0.9% gelling agent concentration in one case each, and solid water with 1.1% gelling agent concentration in two cases. Since three cases were observed with a 3% gelling agent concentration, 200 g is considered appropriate for a 1.3% gelling agent concentration, but 100 g is sufficient for short-distance transportation.

When the dehydration state of the animal was checked by hematocrit, the control showed an increase of 18%, but there was no significant change in each group to which solid water was given, and it is considered that the effect of solid water was obtained. .

Next, in the above test 1. And test 2. Based on the data obtained in, it was examined whether there was a significant difference in the intake amount depending on the jelly strength of the solid water. As shown in Table 3, seven types of gelling agent concentrations were changed as solid water, and sump NO. Prepare 1-7,
The intake amount and its variation in the time periods of 0 to 4 hours, 4 to 8 hours, 8 to 16 hours, and 16 to 24 hours were examined. The results are shown in Table 3 and FIG. Since the jelly strength of each sample is composed of the same component, it corresponds to the gelling agent concentration of each sample.

[0036]

[Table 3]

As described above, before the target animal group is transported by the transport vehicle, it is fasted to prevent vomiting. Therefore, the amount of meal immediately after the supply of the solid water in the transport vehicle increases. However, if a subject animal in a fasted state rapidly ingests water immediately after feeding, it causes diarrhea and vomiting, which is not preferable. In addition, if the target animal ingests almost all of the supplied solid water immediately after feeding, there is a possibility that hydration may not be possible in the latter half of the long transport period. From this, samples 1 and 2 have a large average intake, but the intake time period is the first 0.
It concentrates on ~ 4 hours, which is not desirable. Most preferably, after taking some water immediately after feeding, the intake gradually decreases, and thereafter is stable and high.

As is clear from Table 3 and FIG. 1, the solid water of Samples 3 to 6 has a relatively large intake, and
4 hours, 4 to 8 hours) food amount and 8 to 16 hours, 16 to
It is preferable that the amount of meal for 24 hours has little variation. The jelly strength of the solid water at this time is 100 to 450.
g / cm ^2, more preferably a solid water having a jelly strength in the range of 200-30Og / cm ² was found to be optimal. In addition, the unit of the jelly strength of the solid water in the tables below Table 3 is g / cm ² .

As described above, there is a correlation between the jelly intensity and the ingestion time zone. When the jelly intensity is reduced, the peak value of the ingested amount is obtained relatively early after the supply, but when the jelly intensity is increased, the jelly intensity is increased. It was found that the time period during which this amount of intake peaked was delayed, and that adjustment to an appropriate intensity tended to average the amount of intake in each time period.

From this fact, it has been expected that when the state of being confined in the closed space during transportation is long, the jelly strength can be increased and the intake time can be delayed. In this test, the jelly strength at which the water can be replenished regardless of the transportation time and the ingestion time zone is stable is, in this test example, a gelling agent concentration of 1.1% and a jelly strength of 20%.
It was 0 g / cm ² .

Next, from the results shown in Table 3 and FIG. 1, it was confirmed whether or not there was a difference in the state of improvement of the state of solid water intake and dehydration of the animal depending on the type of the gelling agent to be added. Test 3. Based on the idea that there is a correlation between the jelly strength of solid water and the amount of intake even if the type is changed. And test 4. Was done.

Test 3. Fasted and fasted animals (beagle dogs) were divided into 5 groups as follows, divided into 5 groups, and given solid water containing a gelling agent for 0 hour, 4 hours, 8 hours, and 16 hours.
Intake at 24 hours and 24 hours, blood chemistry tests and body weight measurements were performed. Gelatin 483 manufactured by Miyagi Chemical Co., Ltd. was used as the gelling agent.

Control F1 2% gelatin (equivalent to 70 g / cm ² as jelly strength) F2 4% gelatin (equivalent to 150 g / cm ² as jelly strength) F3 4.5% gelatin (equivalent to 300 g / cm ² as jelly strength) ) F4 5% gelatin (corresponding to 450 g / cm ² as jelly strength) F5 6% gelatin (corresponding to 700 g / cm ² as jelly strength) The results are shown in Table 4.

[0044]

[Table 4]

Test 4 Fasted and fasted animals (beagle dogs) were divided into 5 groups as follows, divided into 5 groups, and given solid water containing a gelling agent for 0 hour, 4 hours, 8 hours, and 16 hours.
Time and 24-hour intake, blood tests and body weight measurements were performed. Pectin 111 manufactured by Miyagi Chemical Co., Ltd. was used as the gelling agent.

Control F1 1% pectin (equivalent to 60 g / cm ² as jelly strength) F2 2% pectin (equivalent to 200 g / cm ² as jelly strength) F3 2.5% pectin (equivalent to 300 g / cm ² as jelly strength) ) F4 3.0% pectin (corresponding to jelly strength of 500 g / cm ² ) F5 3.5% pectin (corresponding to jelly strength of 1000 g / cm ² ) The results are shown in Table 5.

[0047]

[Table 5]

From the results of Tables 4 and 5, it was confirmed that there was no difference in the state of improvement in the state of solid water intake and dehydration of animals even when the type of gelling agent added was changed. The above test 3. And test 4. Tables 6 and 7 were created based on the results shown in Tables 4 and 5 to examine the degree of variation in the correlation between the gelling agent jelly strength and the ingestion time zone. did.

[0049]

[Table 6]

[0050]

[Table 7] Tables 6 and 7 are graphed and shown in FIGS. 2 and 3, respectively.

Test 3. Test 4. In both cases, the same tendency as in the case of using the carrageenan preparation was observed between the jelly intensity and the ingestion time zone, and there was no difference in the ingestion state depending on the type of the gelling agent. Also, when gelatin or pectin was used as the gelling agent, it was confirmed that the optimum jelly strength was in the range of 100-450 g / cm ² , as in the case of using the carrageenan preparation.

From this, it was found that the state of ingestion of the solid water of the present invention was little affected by the type of gelling agent and was affected by the jelly strength. Next, an experiment was conducted to confirm the palatability and intake amount when beef flavor was added to solid water.

Test 5 In the test, a group of fasted and water-fasted animals (beagle dogs) was used as a group, and a carrageenan preparation (Ace Agar AG-103C (Miyagi Chemical Industry Co., Ltd.)) was used as a gelling agent. %
The solidified water divided into a total of 6 groups in which the concentration of the gelling agent and the concentration of the beef flavor A (beef flavor C11765 (Ogawa fragrance)) and the concentration of beef flavor B (beef flavor C11766 (Ogawa fragrance)) were changed. At 0, 4, 8, and 24 hours after the administration, the intake, blood biochemical properties and body weight were measured.

1. 1. Control (no gelling agent and beef flavor added) 2. Blank (only 1.1% gelling agent is added) F1 (1.1% gelling agent + 0.1% beef flavor A) 4. F2 (1.1% gelling agent + 0.3% beef flavor A) 5. F3 (1.1% gelling agent + 0.5% beef flavor A) 6. F4 (1.1% gelling agent + 0.3% beef flavor B) Table 8 shows the results of the intake of each group, the average value of the blood biochemical property test and the weight measurement.

[0055]

[Table 8]

As shown in Table 8, the taste of solid water obtained by adding a gelling agent and beef flavor to solid water was evaluated from 0 to 4.
Hours, 4-8 hours, 8-24 hours and 0-24 hours of intake.

The intake of each group is F for 0 to 4 hours.
1, F2 and F4 had 42-54% of intake, but F3
Had a slightly higher intake of 63%. In addition, the intake of F4 was almost the same as that of the blank (without addition of beef flavor) in 4 to 8 hours, but the intake of F1, F2, and F3 was 4-1.
It was as low as 3%. Eight to twenty-four hours were 15 to 35%, almost the same intake. In addition, F3, F4 in 0-24 hours
Had almost the same intake as the blank, but F1 and F2
Ingestion was low.

As described above, the addition of beef flavor was expected to increase the intake, but the intake of F4 at each time and the intake for 0 to 24 hours was almost the same as that of the blank. When the rate of increase of the hematocrit value was observed, F1 was the same as the blank, but showed an increasing tendency as the addition amounts of F2, F3 and beef flavor increased. F4
Although hematocrit was high, hematocrit was increased.

Test 5 as described above. In this case, the addition of beef flavor was expected to increase the intake, but no effect was observed. In addition, it is generally known that the addition of beef flavor to feed increases the intake. In the beagle dog breeding facility where the test was carried out, no beef flavor addition effect was observed due to favorable conditions, but it can be expected that the beef flavor addition effect can be expected during transportation or in another breeding facility and dog breed. ing. Next, the confirmation test of the palatability and intake amount when the beef flavor was added to the solid water containing the gelling agent was performed again.

Test 6 In the test, a group of fasted and water-fasted animals (beagle dogs) was used as a group, and a carrageenan preparation (Ace Agar AG-103C (Miyagi Chemical Industry Co., Ltd.)) was used as a gelling agent. %
The solid water divided into four groups in which the concentration of the blank of the gelling agent and the concentration of the beef flavor A and the beef flavor B were changed was given, and the intake amounts at 0 hours, 4 hours, 8 hours and 24 hours, blood test, and Body weight was measured.

1. Control 2. 2. Blank (1.1% gelling agent) F1 (1.1% gelling agent + 0.4% beef flavor A) 4. F2 (1.1% gelling agent + 0.6% beef flavor A) 5. F3 (1.1% gelling agent + 0.2% beef flavor B) 6. F4 (1.1% gelling agent + 0.4% beef flavor B) Table 9 shows the results of intake, blood test and average weight measurement of each group.

[0062]

[Table 9]

As shown in Table 9, the intake of each group was 0% to 4 hours, F1 and F3 were 36%, F2 was 66%, and F4 was 97.9. % Were almost ingested.

At 4 to 8 hours, the intake of F2 was almost the same as that of the blank, but the intake of F1 and F3 was as low as 8 to 9%. Since F4 was consumed almost in 0 to 4 hours, 4 to 8
Time intake was low.

For 8 to 24 hours, the intake was 1 to 2% for F1 to F4. In addition, F1 is F0 for 0 to 24 hours and 47 for F3.
%, The intake was low, but F2 was high at 87%, and all F4 was consumed.

As described above, the addition of beef flavor did not increase the intake of F1 and F3, but showed a clear increase in F2 and F4. In particular, F4 consumed 100% of the given amount. I was In addition, there was no particular change in each item (RBC, WBC, Hb, Ht) of the blood biochemical property test.

Previous test Although no increase in intake was observed in the results of the above compared to solid water without beef flavor despite the addition of beef flavor, this test 6. Beef flavors A and B
By increasing the concentration by 0.1% in both cases, a clear increase in the intake was observed in the F2 and F4 groups as compared with the blank.

From the above, it was found that 0.6% beef flavor A was added to 1.1% gelling agent, or 0.4% beef flavor B was added to 1.1% gelling agent. Was found to increase. Next, a confirmation test of palatability and intake when beef flavor was added to the gelling agent-containing solid water was performed.

Test 7. Fasted and water-fasted animals (beagle dogs) were divided into seven groups as follows, and divided into seven groups as described below, and solid water containing a gelling agent (carrageenan preparation (Ace Agar AG-103C (Miyagi Chemical Industry Co., Ltd.)) was given. , 0 hours, 4 hours, 8 hours and 24 hours later, intake, blood tests and body weight measurements were performed.

Control blank (1.1% gelling agent concentration) About 200 g F1 (1.1% gelling agent + 0.4% beef flavor A) About 200 g F2 (1.1% gelling agent + 0.6% beef) Flavor A) about 200 g F3 (1.1% gelling agent + 0.2% beef flavor B) about 200 g F4 (1.1% gelling agent + 0.4% beef flavor B) about 200 g F5 (1.1% gel Agent + 0.4% beef flavor B) About 400 g Test 7. Table 10 shows the results obtained by examining the palatability of the beef flavor for 0 to 4 hours, 4 to 8 hours, 8 to 24 hours, and 0 to 24 hours.

[0071]

[Table 10]

As is clear from Table 10, the intake of each group was 0% to 4 hours, F1 and F3 were 36%, F2 was 66%, and F4 was 9%.
Almost 7.9% had taken it. In addition, the intake of F2 was almost the same as that of the blank in 4 to 8 hours.
The intake of F.3 was as low as 8-9%, and F4 was mostly consumed in 0-4 hours, so the intake was low in 4-8 hours. For 8 to 24 hours, the intake was 1 to 2% for both F1 to F4. For 0 to 24 hours, F1 and F3 are 47
%, The intake was low, but F2 was high at 87%, and all F4 was consumed.

As described above, the addition of beef flavor did not increase the intake of F1 and F3, but showed a clear increase in F2 and F4. In particular, F4 consumed 100% of the given amount. I was

No particular change was observed in each item of the blood biochemical property test. Test 5. In the results of Test 7, no increase in intake was observed despite addition of beef flavor, but Test 7. In both cases, beef flavors A and B were increased in concentration by 0.1%,
2. In the F4 group, a clear increase in the intake was observed as compared with the blank.

From the above, 0.6% of beef flavor A was added to solid water having a gelling agent concentration of 1.1%, or beef flavor B was added to solid water having a gelling agent concentration of 1.1%. An increase in intake can be expected by adding 4%.

When the maximum intake was examined in F5, three individuals who consumed all of the given amounts in 0 to 4 hours,
In terms of time, the amount of the gelling agent was considered to be appropriate around 400 g since the amount of the gelling agent was further increased by three cases and all the amounts given by the six cases.

Test 1 From test 7. Based on the results, solid water as a gelled water replenisher was produced, for example, by the following procedure. In the following solid water production method, a carrageenan preparation was used as a gelling agent.

Ace Agar AG-103, a gelling agent, was stirred with 199.96 g (98.5%) of tap water.
2.23 g (1.1%) of C (Miyagi Chemical Industry Co., Ltd.) is mixed and swelled. 0.81 g of beef flavor C11766 (Ogawa Perfume Co., Ltd.) as a palatability enhancer was mixed with the jelly liquid,
Thereafter, the mixture is heated to completely dissolve the jelly. 203 g of the obtained mixed jelly liquid is filled in a plastic casing (vinylidene chloride film) and tied. The sausage-type filling obtained is treated at 100 ° C. for 50
Sterilize for a minute. This is then cooled at a product temperature of 40 ° C. or lower until the jelly is completely solidified to obtain a solid water product.

It is desirable to store the obtained product at 0 to 10 ° C. to prevent liquefaction of jelly and spoilage of the product. In addition, when high-temperature sterilization is performed, for example, a shelf life period of 6 to 12 months can be set.

In this manner, solid water which is not in an aqueous solution like water but which is resistant to vibration of a transport truck or the like and does not break its shape and which is favorably taken by experimental animals such as beagle dogs can be obtained. Further, the solid water of the present invention is not limited to experimental animals, and can be used as suitable solid water for hunting dogs such as pets and field trial dogs. For example, when a hunting dog is in service (during actual work) during the hunting season, it is possible to improve the hunting art by looking at the situation of the hunting dog when replenishing water and appropriately feeding it.

Further, in this embodiment, a method of filling solid water in a sausage-type bag made of a plastic film, opening the bag immediately before use in a transport vehicle, and placing it in a room with animals is adopted. Alternatively, the solid water of the present invention can be filled in a toothpaste tube-shaped plastic or metal container, and the solid water can be squeezed out of the tube immediately before use to give the animal to the animal. further,
Fill in a predetermined bottle, a container made of metal or ceramic, etc., and take it out with a spoon immediately before use, give it to experimental animals, fill in cans or plastic cups, or fill heat resistant pouches etc. In addition, it may be taken out of the container immediately before feeding and given.

Further, if the sausage-type bag can be refrigerated instead of a plastic film, it can be fed as it is without taking out solid water from the container by using an edible intestinal membrane or the like. You can also.

[0083]

The solid water of the present invention can be supplied as supplementary water for transportation of experimental animals and pets, so that it can be transported for a long time without water deprivation, and stress on animals can be reduced. Promotes the metabolism of to maintain health.
Furthermore, it can also be used as a simple water source for hunting dogs such as pet animals or field trial dogs when there is no water nearby.

[Brief description of the drawings]

FIG. 1 is a graph showing the relationship between the jelly strength of solid water using a carrageenan preparation as a gelling agent according to the present invention and the average intake, and the relationship between the jelly strength and the standard deviation of the average intake.

FIG. 2 is a diagram showing the relationship between the jelly strength of solid water using gelatin as a gelling agent according to the present invention and the average intake, and the relationship between the jelly strength and the standard deviation of the average intake.

FIG. 3 is a diagram showing the relationship between the jelly strength of solid water using pectin as a gelling agent according to the present invention and the average intake, and the relationship between the jelly strength and the standard deviation of the average intake.

Claims (3)

[Claims] 1. An animal hydrate replenishing solid water comprising water and a gelling agent. 2. Jelly strength of 100 to 450 g / cm ²
The solid water for hydration of animals according to claim 1, which is: 3. Use of the solid water for hydration of an animal according to claim 1 for hydration when transporting an experimental animal in a sealed vehicle, transporting a pet animal, using a hunting dog, or training a field trial. Salary method characterized by the following.