JPH11194109A - Usage method for ion sensor and usage method for ion sensor plate as well as ion sensor and ion sensor plate used for them - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an ion sensor in which dispersion stability of a dispersed substance in water such as a blood cell, a protein or the like reflected on a hematocrit or the like in a sample liquid is maintained and in which the concentration of ions can be measured with high accuracy, by a method wherein the sample liquid is set in a state that it contains a surface-active agent until at least the sample liquid is supplied to a sample-liquid measuring electrode. SOLUTION: An ion sensor is composed of a glass epoxy substrate 1, of a sample-liquid measuring electrode 2a, of a silver halide layer 4a, of a silver chloride layer 3 and of the like. The electrode on the side to which a sample liquid is supplied is coated in such a way that a part in a part faced with a through hole 6a at the silver halide layer and the outside end part of the electrode are excluded. In addition, another electrode is coated in the same manner. Actually, an insulating film which covers the surface of a multi-ion sensor plate body 5 excluding respective corresponding parts covers a window part 4c, a dam body 9, the through hole 6a and an ion sensing film 2d. In addition, resin solutions for different ion sensing films are dropped into another through hole 6a in the same manner so as to be dried. As a result, an iron sensitive electrode in which different specific ion sensing films are formed is formed.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an ion sensor for various kinds of ions for examining blood and the like, and more particularly, to a simplified ion sensor plate of a part thereof, for example, a measured value of an ion concentration is influenced by a special component contained in blood. The present invention relates to a method of using an ion sensor and an ion sensor plate which are reduced in the number, and their structures.

[0002]

2. Description of the Related Art Conventionally, some ion sensor plates have been proposed as simplified ion sensor plates for inspection. As one of them, a differential type multi-ion sensor is to connect a multi-ion sensor plate to a measurement circuit and simultaneously measure many different ion concentrations.
As shown in FIG. 2, the multi-ion sensor plate is composed of a pair of a sample liquid measurement electrode 2a and a reference electrode 2b in which a copper electrode formed by etching on a glass epoxy substrate 1 is subjected to electrolytic silver plating. A silver chloride layer 4a on the silver plating layer at the opposite end of each electrode;
4b, a multi-ion sensor plate main body 5 having the ends of the electrodes facing away from each other as external electrodes, and through holes 6a to 6e, 6 'communicating with a set of silver chloride layers 4a, 4b. a to 6′e, a liquid junction 7 of an elongated hole corresponding to tips of two flow paths of an upper cell described later, which contact the sample liquid and the reference liquid, and the outside of each of the electrodes 2a and 2b of each set. A bank 9 made of a polyester film having through holes 8a and 8b communicating with the electrodes is joined to the multi-ion sensor plate body 5. Each through hole 6 of embankment 9
a, 6′a, and various ion-sensitive membrane solutions containing various ion-sensitive substances such as a macrocyclic compound called ionophao or an ion-exchange resin via the elongated holes 6b to 6e are dropped, dried, and dried. A total of five different ion-sensitive films are provided by forming ion-sensitive films on the silver chloride layers 4a and 4b. The joined body of the multi-ion sensor plate body 5 and the bank body 9 is inserted into the concave portions 10a and 10b of the lower cell 10 made of a transparent acrylic resin plate, and the upper cell 12 made of a transparent acrylic resin is attached to the lower cell 10 with a double-sided adhesive tape. And the above joined body is mounted. The upper cell 12 is provided on the rear surface of the transparent plate with the through holes 6a to 6e, 6'a.
Flow paths 13 and 14 composed of concave grooves communicating with to 6′e are provided in two parts by a partition, and one end of each flow path is provided with a sample liquid input section 15 and a reference liquid input section 16,
At the other end of each of the flow passages, air vent grooves 13a and 14a each formed of a narrow groove communicating with the air passage are L-shaped, respectively.
The sample liquid input section 15 and the reference liquid input section 16 communicate with the vertical holes 13b and 14b provided at the ends of the reference liquid input section 16, and the sample liquid measurement electrode 2a and the reference electrode 2b are exposed to the outside. Terminal insertion holes 18a and 18b for inserting terminals of a measuring instrument (not shown) are provided corresponding to the electrodes.

When a multi-ion sensor plate having such a structure is used, the sample liquid and the reference liquid are respectively injected into the through holes formed on the upper surfaces of the sample liquid input section 15 and the reference liquid input section 16 by a syringe or the like. Then, the flow path 13,
14, the respective liquids are circulated and the through holes 6a to 6e, 6 '
While being supplied to the ion-selective electrodes in a to 6′e, the sample liquid and the reference liquid come into contact at the liquid junction 7. In this state, by inserting the terminals of the measuring instrument into the terminal insertion holes 18a and 18b and making contact with the respective external electrodes, a potential corresponding to the concentration of the ionic component of the sample liquid is measured, and this potential is converted into a concentration. The concentration of a total of five types of ions is measured by one injection of the sample liquid and the reference liquid.

[0004]

However, in the measurement of ion concentration in blood, hematocrit (Hc
The influence of a factor called t) (volume ratio of red blood cells in blood) which is considered to reflect the amount of blood cells and blood proteins in blood is considered, but no countermeasures have been taken so far.
Originally, it is ideal to correct the measured value of the ion concentration with reference to this Hct value, but the Hct value varies among individuals such as adult males 42 to 45 and adult females 38 to 42. Since there are various measurement methods, the result is not constant depending on which value is taken by which method, so that the reliability is generally low and this method is not used. Under such circumstances, the in-concentration measurement value using the above multi-ion sensor plate must be used, but the ion measurement is based on hematocrit% (Hct%), that is, the amount of blood cells and blood protein components. The value may indicate an abnormal value. For example, when the Hct is 40 to 50%, the Ca value is about 0.1 to 0.2 mM (millimol, the same applies hereinafter), and the Na value is about 2 to 5 mM, which may be lower or higher than the true value. There is a need for a method of measuring ion concentration and development of an apparatus therefor.

A first object of the present invention is to maintain the dispersion stability of an aqueous dispersion of blood cells and proteins reflected on hematocrit and the like in a sample liquid supplied to a sample liquid measurement electrode during measurement, and to maintain the dispersion stability. An object of the present invention is to provide a method of using an ion sensor, a method of using an ion sensor plate, and an ion sensor and an ion sensor plate used for the same so that an object is prevented from settling on a sample liquid measurement electrode during measurement. A second object of the present invention is to provide a method of using an ion sensor and a method of using an ion sensor in which the measured value of ion concentration does not show an abnormal value due to a change in the dispersion state of a dispersion in water such as blood cells and proteins reflected in hematocrit and the like. An object of the present invention is to provide a method of using a plate, an ion sensor used for the plate, and an ion sensor plate. A third object of the present invention is to
It is to obtain a highly accurate ion concentration measurement value. A fourth object of the present invention is to provide an ion sensor and an ion sensor plate which are easy to operate and easy to use, and which have a simple structure, a simple structure, and a high productivity. It is in.

[0006]

In order to solve the above-mentioned problems, the present invention provides (1) at least one pair of a sample liquid measuring electrode having a coating film of an ion-sensitive film of a specific ion and a reference electrode. In the method of using an ion sensor, wherein an ion sensor plate main body is provided and a measurement circuit is connected to the ion sensor plate main body so that a specific ion concentration of the sample liquid can be measured, a sample supplied to the sample liquid measuring electrode is provided. The present invention provides a method for using an ion sensor in which at least a sample liquid of a liquid and a reference liquid supplied to the reference electrode is supplied in a state containing a surfactant before being supplied to the sample liquid measurement electrode. . Further, the present invention provides (2) a method for using the ion sensor according to the above (1), wherein at least the sample liquid out of the sample liquid and the reference liquid contains a surfactant and is supplied to the sample liquid measuring electrode; An ion sensor plate body having a pair of a sample liquid measurement electrode having a coating film of an ion-sensitive film and a reference electrode and at least one set provided on a substrate; and a closed flow for supplying a sample liquid to the sample liquid measurement electrode. A flow path and a closed flow passage for supplying a reference liquid to the reference electrode are formed on the substrate surface, and an upper cell having a sample liquid input section and a reference liquid input section communicating with the respective flow paths; and the ion sensor In an ion sensor plate having at least a sample liquid provided in the plate main body and supplied to the respective flow passages and a liquid junction part for liquid junction of the reference liquid, the sample liquid supplied to the sample liquid measurement electrode and A method of using an ion sensor plate which is supplied in a state in which at least a sample liquid of the reference liquid supplied to the reference electrode contains a surfactant before being supplied to the sample liquid measurement electrode; The method of using the ion sensor plate according to (3), wherein at least the sample liquid of the reference liquid contains a surfactant and is supplied to the sample liquid measurement electrode;
(5) The ion sensor according to (1), wherein at least the sample liquid supply passage of the sample liquid supply passage for supplying the sample liquid to the sample liquid measurement electrode and the reference liquid supply passage for supplying the reference liquid to the reference electrode. (6) The ion sensor plate according to (3), wherein the surfactant is coated with a surfactant so that a surfactant can be added to the sample liquid along with the flow of the sample liquid. The surfactant is added to at least a part of at least the former one of the closed flow passage for supplying the reference liquid and the closed flow passage for supplying the reference liquid so that a surfactant can be added to the sample liquid along with the flow of the sample liquid. An ion sensor plate provided with a coating of an activator is provided.

[0007]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS A joined body of a multi-ion sensor plate main body 5 and a bank body 9 shown in FIG. 2 is manufactured, and a polyvinyl chloride resin, a plasticizer, and the like are provided in, for example, through holes 6a and 6'a of the bank body.
Ion-sensitive substance, Na-TPB, K-KT as required
Stabilizes the dissociation equilibrium of an addition salt such as an anion eliminator such as PB and the above-mentioned silver halide if necessary, and stabilizes the potential generated at the interface between the silver halide layer of the electrode and the ion-sensitive film. The above-described dissociation equilibrium constituent of silver halide or a substance that dissociates ions thereof, or a potential stabilizing substance of an anion eliminator added in excess, or a combination with at least one of these anion eliminators and other potential stabilizing substances A resin solution for the specific ion-sensitive membrane containing the same type of ion-sensitive membrane and containing an excessive amount of ionophore is dropped and dried to form the ion-sensitive membrane for the specific ion. To form FIG. 1 shows a cross-sectional view around the electrode on the side that supplies the sample liquid. In the figure,
1 is a glass epoxy substrate, 2a is a sample liquid measuring electrode, 4a
2 is a silver halide layer, not shown in FIG. 2, but covers a part of a part of the silver halide layer such as a silver chloride layer which faces the through hole 6a and an outer end of the electrode, The other electrodes not shown are also covered in the same manner.
An insulating film covering the surface of the substrate, 4c is a window, 9 is a bank, 6a
Is a through hole, and 2d is an ion-sensitive membrane. In addition, different resin solutions for ion-sensitive membranes are respectively dropped into the through holes 6b and 6'b, 6c and 6'c, 6d and 6'd, and 6e and 6'e in the same manner as described above, and dried, respectively. An ion-sensitive electrode having another specific ion-sensitive film formed thereon is formed.

Next, as in the case of FIG. 2, the joined body of the multi-ion sensor plate main body 5 and the bank body 9 is inserted into the recesses 10a and 10b of the lower cell 10 made of a transparent acrylic resin plate. Then, the upper cell 12 is joined with a double-sided adhesive tape, and the joined body is installed. At this time, as shown in FIG. 1, a surfactant is added to the sample liquid when the sample liquid is passed through at least the former of the sample liquid flow path and the reference liquid flow path. An aqueous solution of a surfactant is applied so that the amount to be applied is appropriately adjusted depending on the type of the surfactant, and the surfactant coating 1
9 is used. A sample liquid and a reference liquid are respectively injected into the sample liquid input section 15 and the reference liquid input section 16 of the multi-ion sensor plate having such a structure by a syringe or the like, and the respective liquids are circulated through the flow passages 13 and 14. The liquid is supplied to the ion-sensitive electrodes in the through-holes 6a to 6e and 6'a to 6'e, and the two are brought into contact at the liquid junction 7 so that electrical conduction can be achieved. Then, the terminals of the measuring instrument, not shown, are inserted into the terminal insertion holes 18a, 1a.
By inserting the sample into the sample electrode 8b and bringing the sample into contact with an external electrode of the electrode, five different ion concentrations in the sample solution can be measured.

In the above, the surfactant was applied to the flow passages 13 and 14, but the multi-ion sensor plate was prepared in the same manner as above except that the upper cell of FIG. Prepare and use this, and use a mixture in which a surfactant is added to at least the sample solution of the sample solution and the reference solution, and the amount of the surfactant added is appropriately adjusted depending on the type of the surfactant. Then, the measurement may be performed in the same manner as described above.

As described above, not only when the surfactant is contained in the sample liquid but also when the surfactant is coated in the flow passage of the multi-ion sensor plate, the surfactant is not changed. This improves the dispersibility of blood cells and proteins, which occurs when the dispersibility is poor.The blood cells and proteins precipitate on the ion-sensitive membrane and adhere to the surface by Can be shielded to prevent the ions from contacting the ion-sensitive membrane. As described above, since it is only necessary to apply the surfactant to the flow passage or simply add the surfactant to the sample solution, the operation is easy, the structure is simple, and the conventional ion sensor,
The use and manufacturing method of the ion sensor plate can be applied almost as it is without any major change. The “surfactant” may be referred to as a “surfactant that maintains the dispersibility of a hydrophobic substance (hematocrit or a similar substance) contained in a sample liquid and prevents sedimentation (precipitation)”. When a surfactant is applied to the flow path of the reference liquid so that the surfactant can be added to the flow of the reference liquid, the conditions for comparison are the same as when the flow path of the sample liquid was the same. Can be Also, when the surfactant is contained in the reference solution, the comparison conditions can be made constant as compared with the case where the same is applied to the sample solution.

Examples of such surfactants include alkyl sulfates, alkylbenzene sulfonates, alkyl naphthalene sulfonates, alkyl sulfosuccinates,
Alkyl diphenyl ether disulfonate, alkyl phosphate, polyoxyethylene alkyl or alkyl allyl sulfate, naphthalene sulfonic acid formalin condensate, special polycarboxylic acid type polymer surfactant, polyoxyethylene alkyl phosphate and other shades Ionic surfactant, polyoxyethylene alkyl ether,
Polyoxyethylene alkyl allyl ether, polyoxyethylene derivative, oxyethylene / oxypropylene block polymer, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene sorbitol fatty acid ester, glycerin fatty acid ester, polyoxyethylene fatty acid ester, polyoxy Examples include at least one of ethylene alkylamines and other nonionic surfactants, alkylamine salts, quaternary ammonium salts, alkylbetaines, amine oxides and other cationic surfactants, and amphoteric surfactants.
If a surfactant containing an ion to be detected is used, it is difficult to obtain a true value of the ion concentration in the sample liquid, and thus it is preferable not to use the surfactant.

As a potential stabilizing substance, Cl^-Source of supply and
The general formula R_FourN⁺Cl^-(R represents an alkyl group,
The same applies hereinafter. An ammonium salt represented by the general formula R_FourP
⁺Cl^-At least one of the phosphonium salts represented by
And Cl^-Besides Br ^-, I^-But the same effect
These ammonium salts, phosphonium
And a compound corresponding to a salt of the compound. Also on
As the polyvinyl chloride resin described above, vinyl chloride resin,
Vinyl chloride and vinyl acetate, vinyl alcohol, carbox
At least the amount of sil group-containing monomer and other vinyl monomers
Are also copolymers with one kind.
Phthalic acid plasticizers, aliphatic dibasic acid plasticizers,
Ethers of substituted or unsubstituted phenols with higher alcohols
And other plasticizers.

[0013]

Next, an embodiment of the present invention will be described with reference to the drawings. Example 1 is a plasticizer DOS (sebacic acid (2-ethylhexyl)) quaternary amine salts such as trioctyl methyl ammonium chloride in 2 g ^- chlorine (Cl sources) 0.8
mg of AgCl (potential stabilizing substance)
Add 10 mg and stir. Supernatant 25 of the mixture
0 mg was collected, and PVC (vinyl chloride: vinyl alcohol: vinyl acetate = 90: 6: manufactured by Denki Kagaku Kogyo Co., Ltd.) was added thereto.
155 mg, ETH1001 (ionophore for detecting calcium ions) 8.6 mg, K-TCP
B (potassium tetrakis (p-chlorophenyl)
Borate, Dojin Chemical Co., anion exclusion agent) 1.24m
g, and 250 ml of THF (tetrahydrofuran) as a solvent is further added to obtain a resin solution for an ion-sensitive membrane.
The supernatant contains a quaternary amine salt and AgCl, but these are small and are ignored in quantity.
0 mg, which can be approximated to 60% by weight in the nonvolatile matter (ion sensitive membrane). The resin solution for the ion-sensitive membrane was used as described above with reference to FIGS.
It was applied by dripping onto silver chloride layers 4a and 4b through 6'a, and was naturally dried to form a calcium ion-sensitive film as ion-sensitive film 2d. Next, as shown in FIG. 1, the inner surfaces of the flow passages 13 and 14 of the upper cell 12 (flow passage 1
3 is shown, but the inner surface of the flow passage 14 is also the same).
0.1 g of KF-6004 (terminal reactive silicone oil) (a nonionic surfactant manufactured by Shin-Etsu Chemical Co., Ltd.)
/ Liter of solution (ethanol solution)
After drying, a coating film 19 of a surfactant is formed. A multi-ion sensor plate having the same structure as that shown in FIGS. 1 and 2 was prepared except for this, and the same plurality of specimens were used except that hematocrit% (Hct%) in blood was changed. The result of measuring the calcium ion concentration of the liquid is shown in FIG. 3 by a solid line. The sample liquid was prepared by centrifuging whole blood of a horse (Thoroughbred) into blood cells and plasma, changing the amount of plasma to prepare a sample, and measuring this by a capillary method to obtain Hct%. Hc shown in the figure
Different t% values were obtained.

Comparative Example 1 In Example 1, as the upper cell 12 shown in FIG. 2, no surfactant was applied to the inner surfaces of both the flow passages 13 and 14, and the state of the inner surface of the molded product at the beginning was maintained. The result of measuring the calcium ion concentration in the same manner except that the calcium ion concentration was used is shown by a dotted line in FIG.

Example 2 In Example 1, as in Comparative Example 1, no surfactant was applied to the inner surface of each of the flow passages 13 and 14 as the upper cell 12, and the state of the inner surface at the beginning of the molded product was obtained. 100 KF-6004 surfactant was added to each sample solution.
The results obtained by measuring the calcium ion concentration in the same manner except that the mixture was added so that the concentration became mg / L, shaken, stirred and mixed were used, and the results are shown by the solid line in FIG.

Comparative Example 2 The results obtained by measuring the calcium ion concentration in Example 2 in the same manner as in Example 2 except that no KF-6004 surfactant was added to each sample solution are shown by the dotted line in FIG. . The solid line and the dotted line in FIGS. 3 and 4 are obtained by taking two data for each Hct% value and connecting the average values.

Example 3 2 g of a plasticizer, DOA (dioctyl adipate), was added to a fourth resin such as trioctylmethylammonium chloride.
Grade salt ^- was added 0.8mg chlorine (Cl sources), further AgCl (potential stabilizing substance) 10 mg of powder was added and stirred. 250 mg of the supernatant of the mixed solution was separated, 155 mg of PVC (copolymer of vinyl chloride: vinyl alcohol: vinyl acetate = 90: 6: 4, manufactured by Denki Kagaku Kogyo KK), and valinomycin (ionophore for potassium ion detection). ) 14 mg, 1.24 mg of K-TCPB (potassium tetrakis (p-chlorophenyl) borate, manufactured by Dojin Chemical Co., Ltd., anion exclusion agent), 250 ml of THF (tetrahydrofuran) as a solvent was added, and the resin solution for ion-sensitive membrane was added. I do. The supernatant contains a quaternary amine salt and AgCl, but since these are small, they are neglected in terms of quantity and can be almost 250 mg of a plasticizer, which is about 60% by weight in the nonvolatile matter (ion-sensitive membrane). it can. This ion-sensitive membrane resin solution was supplied to the silver chloride layers 4a and 4a through the through holes 6a and 6'a in the same manner as in Example 1.
Then, the mixture was applied by dripping to b, and dried naturally to form a potassium ion-sensitive film as the ion-sensitive film 2d. Except for this, a multi-ion sensor plate was prepared in the same manner as in Example 1, and the potassium ion concentration was measured. The result is shown by a solid line in FIG.

Comparative Example 3 In Example 3, as in Comparative Example 1, no surfactant was applied to the inner surfaces of both the flow passages 13 and 14 as the upper cell 12, and the state of the inner surface at the beginning of the molded product was obtained. The results of measuring the potassium ion concentration in the same manner except that it was used as it is are shown by the dotted line in FIG.

[0019] Example 4 is a plasticizer NPOE (2-nitrophenyl octyl ether) 2 g quaternary amine salts such as trioctyl methyl ammonium chloride ^- chlorine (Cl sources) 0.
8 mg was added, and 10 mg of powdered AgCl (potential stabilizing substance) was further added, followed by stirring. A supernatant (250 mg) of the mixed solution was separated and added to PVC (vinyl chloride: vinyl alcohol: vinyl acetate = 90: manufactured by Denki Kagaku Kogyo Co., Ltd.).
6: 4 copolymer) 190 mg, bis (12-crown-4) (ionophore for sodium detection) 12.4 m
g, 0.86 mg of Na-TPB (sodium tetrakisphenylborate, manufactured by Dojin Chemical Co., Ltd., anion exclusion agent)
And the solvent THF (tetrahydrofuran)
Add 250 ml to make a resin solution for ion-sensitive membrane. The composition of the non-volatile components except for THF in the resin solution for the ion-sensitive membrane and the proportion of NPOE in the non-volatile components was 55% by weight. The resin solution for the ion-sensitive membrane was applied to the silver chloride layer 4a through the through holes 6a and 6'a in the same manner as in Example 1.
4b, it was applied by dripping and air-dried to form a sodium ion-sensitive film as the ion-sensitive film 2d. Except for this, a multi-ion sensor plate was prepared in the same manner as in Example 1, and the result of measuring the sodium ion concentration is shown by a solid line in FIG.

Comparative Example 4 In Example 4, as in Comparative Example 1, as the upper cell 12, no surfactant was applied to any of the inner surfaces of the flow passages 13 and 14, and the state of the inner surface of the molded product at the beginning was obtained. The result of measuring the sodium ion concentration in the same manner except that it was used as it is is shown by a dotted line in FIG. The solid line and dotted line in FIGS. 5 and 6 are obtained by taking five data points for each Hct% value and connecting the average values.

From FIGS. 3 to 6, the measured ion concentration was obtained regardless of the Hct% value in both the case where the surfactant was applied to the flow passage of the upper cell and the case where the surfactant was contained in the sample solution. Is constant, but Comparative Examples 1 and 2 shown in FIGS.
In FIG. 5, the measured calcium ion concentration decreases in substantially the same manner as the Hct% value increases, and in the comparative examples shown in FIGS. 5 and 6, the measured ion concentrations increase with the increase in the Hct% value. In the examples, the effect of using a surfactant is remarkably exhibited. 3 and 4, H
When the ct% value is 40 to 45% or more, the difference starts to increase, and FIG.
In the case of No. 6, the difference increases when the Hct% value exceeds 20%. From these facts, the above (1) to (6)
In other inventions, the Hct% value is at least 20% (20% or more) when detecting potassium and sodium ion concentrations, and the Hct% value is at least 40% (40%) when calcium ion concentration is detected.
% Or more of the sample solution, and the Hct% value as a whole is at least 20% (20% or more).
As described above, the use of at least 40% (40% or more) of the sample liquid may be limited.

[0022]

According to the present invention, at least the sample liquid is made to contain the surfactant before the sample liquid is supplied to the sample liquid measuring electrode, so that it is supplied to the sample liquid measuring electrode at the time of measurement. Maintains the dispersion stability of the aqueous dispersion of blood cells and proteins reflected in the hematocrit and the like in the sample liquid, and does not precipitate on the sample liquid measurement electrode during measurement, thereby showing no abnormal value and high accuracy. It is possible to provide not only a method of using an ion sensor and a method of using an ion sensor plate capable of obtaining an ion concentration measurement value, but also an ion sensor and an ion sensor plate for that purpose. Not only can provide an easy-to-use method of using an ion sensor and an easy-to-use method of using an ion sensor plate, but also have a simple structure. , It is possible to provide a productivity is good ion sensor and ion sensor plate.

[Brief description of the drawings]

FIG. 1 is a partial sectional view of a multi-ion sensor plate according to one embodiment of the present invention.

FIG. 2 is an exploded perspective view of a multi-ion sensor plate.

FIG. 3 is a graph showing a measured value of a calcium ion concentration in a sample liquid measured using the multi-ion sensor plate of the example and a comparative example.

FIG. 4 is a graph showing a measured value of a calcium ion concentration in a sample liquid of another example using a multi-ion sensor plate and a comparative example thereof.

FIG. 5 is a graph showing a measured value of a potassium ion concentration in a sample solution measured using a multi-ion sensor plate of another example and a comparative example thereof.

FIG. 6 is a graph showing a measured value of a sodium ion concentration in a sample liquid measured using a multi-ion sensor plate of still another example and a comparative example thereof.

[Explanation of symbols]

 2a Sample liquid measurement electrode 2b Reference electrode 2d Ion-sensitive membrane 5 Multi-sensor plate body 7 Liquid junction 13, 14 Flow path 19 Surfactant coating

 ──────────────────────────────────────────────────の Continuing on the front page (72) Inventor Akihiko Mochizuki Taiyo Denki Co., Ltd. 6-16-20 Ueno, Taito-ku, Tokyo

Claims (6)

[Claims] 1. An ion sensor plate main body having at least one pair of a sample liquid measurement electrode having a coating film of an ion-sensitive membrane of a specific ion and a reference electrode is provided, and a measurement circuit is connected to the ion sensor plate main body. In the method of using an ion sensor, which can measure a specific ion concentration of a sample liquid, at least the sample liquid of the sample liquid supplied to the sample liquid measurement electrode and the reference liquid supplied to the reference electrode is the sample liquid. A method of using an ion sensor which is supplied in a state containing a surfactant before being supplied to a measurement electrode. 2. The method according to claim 1, wherein at least one of the sample liquid and the reference liquid contains a surfactant and is supplied to a sample liquid measurement electrode. 3. An ion sensor plate main body comprising a pair of a sample liquid measurement electrode having a coating film of an ion-sensitive film and a reference electrode provided on a substrate, and supplying a sample liquid to the sample liquid measurement electrode. Forming a closed flow path for supplying a reference liquid to the reference electrode and a closed flow path for supplying a reference liquid to the reference electrode, and a sample liquid input unit communicating with each flow path;
In the ion sensor plate having at least an upper cell having a reference liquid input portion, and a sample liquid provided in the ion sensor plate main body and supplied to the respective flow passages, and a liquid junction for liquid junction of the reference liquid, At least the sample liquid of the sample liquid supplied to the liquid measurement electrode and the reference liquid supplied to the reference electrode is supplied in a state containing a surfactant before being supplied to the sample liquid measurement electrode. How to use 4. The method according to claim 3, wherein at least the sample liquid of the sample liquid and the reference liquid is supplied to a sample liquid measuring electrode after containing a surfactant. 5. The ion sensor according to claim 1, wherein at least one of a sample liquid supply passage for supplying a sample liquid to the sample liquid measurement electrode and a reference liquid supply passage for supplying a reference liquid to the reference electrode. An ion sensor in which a surfactant coating is provided in a passage so that a surfactant can be added to the sample liquid as the sample liquid flows. 6. The ion sensor plate according to claim 3, wherein at least a part of at least the former of the closed flow passage for supplying the sample liquid and the closed flow passage for supplying the reference liquid. An ion sensor plate provided with a surfactant coating so that a surfactant can be added to the sample liquid as the sample liquid flows.