JPH10510985A - 軟骨細胞株 - Google Patents
軟骨細胞株Info
- Publication number
- JPH10510985A JPH10510985A JP8518391A JP51839196A JPH10510985A JP H10510985 A JPH10510985 A JP H10510985A JP 8518391 A JP8518391 A JP 8518391A JP 51839196 A JP51839196 A JP 51839196A JP H10510985 A JPH10510985 A JP H10510985A
- Authority
- JP
- Japan
- Prior art keywords
- chondrocytes
- cell line
- chondrocyte
- oncogene
- articular
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3817—Cartilage-forming cells, e.g. pre-chondrocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
- A61L27/3843—Connective tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0655—Chondrocytes; Cartilage
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/02—Drug screening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/04—Immortalised cells
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.マトリックス石灰化のための細胞モデルとしての関節軟骨細胞の使用。 2.前記大多数の軟骨細胞がペレット形態に配列される、請求項1に記載の関節 細胞軟骨の使用。 3.前記軟骨細胞は、成熟しているか、または成熟した個体および好ましくは関 節炎を患うかもしくは関節炎の素因を有する個体から誘導される、請求項1また は2に記載の関節軟骨細胞の使用。 4.前記軟骨細胞が胎児起源である、請求項1または2に記載の関節軟骨細胞の 使用。 5.前記軟骨細胞が細胞株として提供される、請求項1から4に記載の関節軟骨 細胞の使用。 6.前記細胞株がヒト起源である、請求項5に記載の関節軟骨細胞の使用。 7.前記軟骨細胞がオンコジーンを含有する、請求項1から6のいずれかに記載 の関節軟骨細胞の使用。 8.前記オンコジーンが温度感受性である、請求項7に記載の関節軟骨細胞の使 用。 9.前記オンコジーンがSV40T抗原である、請求項8に記載の関節軟骨細胞の使 用。 10.カルシウムを鉱化する関節軟骨細胞株を生成するための方法であって、以 下の工程: a)不死化剤を用いて少なくとも1つの前駆体ヒト関節軟骨細胞を不死化する工 程;および b)該不死化細胞を、ヒト関節軟骨細胞の集団を生成するために培養する工程 を包含する、方法。 11.前記不死化剤がオンコジーンである、請求項10に記載の方法。 12.前記オンコジーンが温度感受性である、請求項11に記載の方法。 13.前記オンコジーンがSV40T抗原である、請求項12に記載の方法。 14.前記方法が、前記不死化細胞を、前記オンコジーンの非許容温度で培養す る工程をさらに包含する、請求項12または13に記載の方法。 15.関節炎の処置のために設計された治療薬剤を同定するための、カルシウム を鉱化する少なくとも1つの関節軟骨細胞の使用。 16.関節炎を処置するために設計された治療薬剤を開発するための方法であっ て、以下の工程: a)カルシウムを鉱化する関節軟骨細胞を含有する、軟骨細胞、または少なくと も1つの軟骨細胞株を少なくとも1つの試験薬剤に曝す工程;および b)該試験薬剤に対して応答する性質を観察する工程 を包含する、方法。 17.前記観察が、前記軟骨細胞または前記軟骨細胞株により結合したカルシウ ムの鉱化作用量を評価する工程を包含する、請求項16に記載の方法。 18.前記細胞株が胎児の起源である、ヒト肥大性軟骨細胞株。 19.前記細胞株がオンコジーンを含有する、請求項18に記載の細胞株。 20.前記オンコジーンが温度感受性である、請求項19に記載の細胞株。 21.前記オンコジーンがSV40T抗原である、請求項19または20に記載の細 胞株。 22.ヒト肥大性軟骨細胞株を生成するための方法であって、以下の工程: a)不死化剤を用いて、少なくとも1つのヒト胎児軟骨細胞を不死化する工程、 および b)該不死化細胞を、ヒト胎児由来軟骨細胞の集団を生成するために培養する工 程 を包含する、方法。 23.前記不死化剤がオンコジーンである、請求項22に記載の方法。 24.前記オンコジーンが温度感受性である、請求項23に記載の方法。 25.前記オンコジーンがSV40T抗原である、請求項23または24に記載の方 法。 26.前記方法が、前記不死化細胞を前記オンコジーンの非許容温度で培養する 工程をさらに包含する、請求項24または25に記載の方法。 27.結合マトリックス組織を提供するための使用のために、肥大性軟骨細胞、 または少なくとも1つの肥大性軟骨細胞株の使用。 28.損傷組織の修復および/または配置に使用するための結合マトリックス組 織を生成するための方法であって、以下の工程: a)肥大性軟骨細胞株を提供する工程; b)該細胞株を、マトリックス増殖を促進する条件で培養する工程;および c)該細胞、および/または該マトリックス組織、および/またはその選択され た部分を回収する工程 を包含する、方法。 29.関節炎の処置のために設計された治療薬剤の同定に使用するための、肥大 性軟骨細胞または少なくとも1つの肥大性軟骨細胞株。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB9425071.9A GB9425071D0 (en) | 1994-12-13 | 1994-12-13 | Chondrocyte cell lines |
GB9425071.9 | 1994-12-13 | ||
PCT/GB1995/002497 WO1996018728A1 (en) | 1994-12-13 | 1995-10-25 | Chondrocyte cell-lines |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2002310323A Division JP2003116533A (ja) | 1994-12-13 | 2002-10-24 | 軟骨細胞株 |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH10510985A true JPH10510985A (ja) | 1998-10-27 |
Family
ID=10765824
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP8518391A Withdrawn JPH10510985A (ja) | 1994-12-13 | 1995-10-25 | 軟骨細胞株 |
JP2002310323A Withdrawn JP2003116533A (ja) | 1994-12-13 | 2002-10-24 | 軟骨細胞株 |
JP2005205003A Pending JP2005312996A (ja) | 1994-12-13 | 2005-07-13 | 軟骨細胞株 |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2002310323A Withdrawn JP2003116533A (ja) | 1994-12-13 | 2002-10-24 | 軟骨細胞株 |
JP2005205003A Pending JP2005312996A (ja) | 1994-12-13 | 2005-07-13 | 軟骨細胞株 |
Country Status (15)
Country | Link |
---|---|
EP (2) | EP0795008B1 (ja) |
JP (3) | JPH10510985A (ja) |
KR (1) | KR980700410A (ja) |
AT (1) | ATE248218T1 (ja) |
AU (1) | AU3703795A (ja) |
CA (1) | CA2207764A1 (ja) |
CZ (1) | CZ182497A3 (ja) |
DE (1) | DE69531627T2 (ja) |
DK (1) | DK0795008T3 (ja) |
ES (1) | ES2206518T3 (ja) |
GB (1) | GB9425071D0 (ja) |
HU (1) | HU221244B1 (ja) |
MX (1) | MX9704296A (ja) |
PT (1) | PT795008E (ja) |
WO (1) | WO1996018728A1 (ja) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2002366602B2 (en) | 2001-12-07 | 2008-07-03 | Asterias Biotherapeutics, Inc. | Chondrocyte precursors derived from human embryonic stem cells |
JP2006289062A (ja) * | 2005-03-18 | 2006-10-26 | Pentax Corp | 肥大化能を有する軟骨細胞と足場を用いた骨補填材料 |
CA2828315A1 (en) | 2010-04-08 | 2011-10-13 | The University Court Of The University Of Edinburgh | Chondrogenic progenitor cells, protocol for derivation of cells and uses thereof |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994009118A1 (en) * | 1992-10-13 | 1994-04-28 | The General Hospital Corporation | Immortalized human chondrocytes |
-
1994
- 1994-12-13 GB GBGB9425071.9A patent/GB9425071D0/en active Pending
-
1995
- 1995-10-25 EP EP95934723A patent/EP0795008B1/en not_active Expired - Lifetime
- 1995-10-25 MX MX9704296A patent/MX9704296A/es unknown
- 1995-10-25 AU AU37037/95A patent/AU3703795A/en not_active Abandoned
- 1995-10-25 WO PCT/GB1995/002497 patent/WO1996018728A1/en not_active Application Discontinuation
- 1995-10-25 KR KR1019970703945A patent/KR980700410A/ko not_active Application Discontinuation
- 1995-10-25 DE DE69531627T patent/DE69531627T2/de not_active Expired - Fee Related
- 1995-10-25 CA CA002207764A patent/CA2207764A1/en not_active Abandoned
- 1995-10-25 HU HU9702182A patent/HU221244B1/hu not_active IP Right Cessation
- 1995-10-25 EP EP01110823A patent/EP1142989A3/en not_active Withdrawn
- 1995-10-25 ES ES95934723T patent/ES2206518T3/es not_active Expired - Lifetime
- 1995-10-25 JP JP8518391A patent/JPH10510985A/ja not_active Withdrawn
- 1995-10-25 AT AT95934723T patent/ATE248218T1/de not_active IP Right Cessation
- 1995-10-25 PT PT95934723T patent/PT795008E/pt unknown
- 1995-10-25 DK DK95934723T patent/DK0795008T3/da active
- 1995-10-25 CZ CZ971824A patent/CZ182497A3/cs unknown
-
2002
- 2002-10-24 JP JP2002310323A patent/JP2003116533A/ja not_active Withdrawn
-
2005
- 2005-07-13 JP JP2005205003A patent/JP2005312996A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
MX9704296A (es) | 1998-02-28 |
EP1142989A2 (en) | 2001-10-10 |
HU221244B1 (en) | 2002-09-28 |
CA2207764A1 (en) | 1996-06-20 |
CZ182497A3 (en) | 1997-11-12 |
ATE248218T1 (de) | 2003-09-15 |
DE69531627D1 (de) | 2003-10-02 |
ES2206518T3 (es) | 2004-05-16 |
JP2005312996A (ja) | 2005-11-10 |
GB9425071D0 (en) | 1995-02-08 |
DE69531627T2 (de) | 2004-06-17 |
AU3703795A (en) | 1996-07-03 |
EP1142989A3 (en) | 2001-11-21 |
WO1996018728A1 (en) | 1996-06-20 |
EP0795008A1 (en) | 1997-09-17 |
KR980700410A (ko) | 1998-03-30 |
JP2003116533A (ja) | 2003-04-22 |
PT795008E (pt) | 2004-01-30 |
HUT77076A (hu) | 1998-03-02 |
EP0795008B1 (en) | 2003-08-27 |
DK0795008T3 (da) | 2003-12-15 |
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