JPH10332568A - Particle counting apparatus - Google Patents
Particle counting apparatusInfo
- Publication number
- JPH10332568A JPH10332568A JP9137137A JP13713797A JPH10332568A JP H10332568 A JPH10332568 A JP H10332568A JP 9137137 A JP9137137 A JP 9137137A JP 13713797 A JP13713797 A JP 13713797A JP H10332568 A JPH10332568 A JP H10332568A
- Authority
- JP
- Japan
- Prior art keywords
- reagent
- particles
- filter part
- bacteria
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002245 particle Substances 0.000 title claims abstract description 35
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 75
- 239000012528 membrane Substances 0.000 claims abstract description 44
- 241000894006 Bacteria Species 0.000 claims abstract description 17
- 239000012510 hollow fiber Substances 0.000 claims abstract description 15
- 238000005259 measurement Methods 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 abstract description 15
- 210000002700 urine Anatomy 0.000 abstract description 5
- 238000001914 filtration Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 2
- 230000001934 delay Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 22
- 210000004027 cell Anatomy 0.000 description 11
- 239000007789 gas Substances 0.000 description 5
- 239000012535 impurity Substances 0.000 description 5
- 230000008878 coupling Effects 0.000 description 4
- 238000010168 coupling process Methods 0.000 description 4
- 238000005859 coupling reaction Methods 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229920013716 polyethylene resin Polymers 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000004952 Polyamide Substances 0.000 description 1
- 239000004721 Polyphenylene oxide Substances 0.000 description 1
- 229920002396 Polyurea Polymers 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 239000004760 aramid Substances 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- 229920003235 aromatic polyamide Polymers 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000036046 immunoreaction Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000001471 micro-filtration Methods 0.000 description 1
- JRZJOMJEPLMPRA-UHFFFAOYSA-N olefin Natural products CCCCCCCC=C JRZJOMJEPLMPRA-UHFFFAOYSA-N 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229920002492 poly(sulfone) Polymers 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 238000001223 reverse osmosis Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】この発明は粒子計数装置に関
し、更に詳しくは液体試料を計測部で流して試料中の粒
子の大きさ等を計測する装置に関し、特に血液や尿など
を試料として計測する粒子計数装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a particle counting apparatus, and more particularly to an apparatus for measuring the size of particles in a liquid sample by flowing the liquid sample in a measuring section, and particularly for measuring blood, urine, etc. as a sample. The present invention relates to a particle counting device.
【０００２】[0002]
【従来の技術】この種の粒子計数装置は、試料中の計測
対象を希釈液等の試薬にて適当な濃度に希釈して、アパ
ーチャやフローセル内を流し、電気信号や光学信号を用
いて計数している。場合によっては、試薬は染色反応さ
せたり、免疫反応させたりしてするものが用いられ、計
測部にも試薬で試料を包むようにして細い流れに収束さ
せながら計測する方法が用いられている。臨床検査の分
野でも血液や尿などの試料を用いて、その試料中の細胞
等の粒子の数や大きさなどの計測にひろく使用されてい
る。2. Description of the Related Art A particle counting apparatus of this type dilutes an object to be measured in a sample to an appropriate concentration with a reagent such as a diluent, flows the sample through an aperture or a flow cell, and performs counting using an electric signal or an optical signal. doing. In some cases, a reagent that causes a staining reaction or an immunoreaction is used, and a method of wrapping the sample with the reagent and converging it into a thin flow is used for the measurement unit. In the field of clinical examinations, it is widely used for measuring the number and size of particles such as cells in a sample using the sample such as blood or urine.
【０００３】この計測の際、試料とともに計測部を流す
試薬（希釈液や試薬）に不純物、特に計測対象物と同じ
位の大きさの不純物が混入していると正確な計測が難し
くなる。そこで、試薬容器と計測部の間にフィルター部
を介接させて、不純物を除去することが知られている
（例えば特公平５−８７７７９号）。At the time of this measurement, accurate measurement becomes difficult if impurities (particularly impurities of the same size as the object to be measured) are mixed in the reagent (diluent or reagent) flowing through the measuring section together with the sample. Therefore, it is known that a filter section is interposed between the reagent container and the measurement section to remove impurities (for example, Japanese Patent Publication No. 5-87779).
【０００４】しかしながら、このフィルター部に不純物
がたまってくるとフィルターがつまってしまい試薬が供
給できなくなるので、その際は交換しなくてはならな
い。特に、計測対象物が微小なもの（例えば血小板や細
菌など）であれば、フィルターの目の大きさをそれより
も小さくする必要があり、そうなるとフィルターの交換
時期はかなり短くなり、常にフィルターの管理をしなけ
ればならない。However, if impurities accumulate in the filter section, the filter becomes clogged and the reagent cannot be supplied. In this case, the filter must be replaced. In particular, if the object to be measured is very small (such as platelets or bacteria), it is necessary to make the size of the filter eye smaller than that. Have to do.
【０００５】そこでこの発明の主要な目的の１つは、フ
ィルター部の寿命を長くすることができる粒子計数装置
の提供にある。Therefore, one of the main objects of the present invention is to provide a particle counting device capable of extending the life of a filter section.
【０００６】[0006]
【課題を解決するための手段】この発明によれば、半透
過膜で仕切られた試薬流入側の試薬流出口付近に放出口
を備えたフィルター部を、試薬容器と計測部との間に備
えた粒子計数装置において、試薬流出口より試薬を加圧
し、加圧された試薬を放出口より放出することによっ
て、半透過膜に付着した細菌等の粒子および気泡を除く
よう構成されたことを特徴とする粒子計数装置が提供さ
れる。According to the present invention, a filter section having a discharge port near the reagent outlet on the reagent inflow side partitioned by a semi-permeable membrane is provided between the reagent container and the measuring section. In the particle counting device, the reagent is pressurized from the reagent outlet, and the pressurized reagent is discharged from the discharge port to remove particles such as bacteria and air bubbles attached to the semipermeable membrane. Is provided.
【０００７】すなわちこの発明は、試薬をフィルター部
で濾過するときとは逆に、フィルター部の試薬流出口よ
り試薬を加圧し、それによって半透過膜の試薬流入側面
に付着した細菌等の粒子や気泡を放出口より除き、半透
過膜の濾過効果の低下を遅らせてフィルター部の寿命を
延ばそうとするものである。ここでフィルター部の試薬
流出口より試薬を具体的に加圧する手段としては、計測
部とフィルター部の半透過膜で仕切られた試薬流出口
（低圧側部分）との間に３方切換弁を設け、切換えポー
トに試薬を切換え加圧する加圧空気源（通常０．３〜
０．６ｋｇ／ｃｍ2）を接続するのが、構成が簡単にな
るので好ましい。That is, according to the present invention, in contrast to the case where the reagent is filtered through the filter section, the reagent is pressurized from the reagent outlet of the filter section, whereby particles such as bacteria and the like adhered to the reagent inflow side of the semipermeable membrane. The purpose is to remove bubbles from the outlet and delay the reduction of the filtration effect of the semipermeable membrane to extend the life of the filter. Here, as means for specifically pressurizing the reagent from the reagent outlet of the filter part, a three-way switching valve is provided between the measuring part and the reagent outlet (low-pressure side part) partitioned by the semi-permeable membrane of the filter part. A pressurized air source (normally 0.3 to
0.6 kg / cm 2 ) is preferable because the configuration is simplified.
【０００８】またフィルター部の半透過膜としては、通
常精密濾過膜、限外濾過膜、逆浸透膜などと称される分
離膜が使用でき、材質として芳香族ポリアミド、アリル
−アルキルポリアミド／ポリ尿素、ポリピペラジンアミ
ド、酢酸セルロース、架橋セルロース、架橋ポリエーテ
ル、スルホン化ポリスルホンなどが採用できる。そして
半透過膜の形状としては中空糸膜が大きな濾過面積をコ
ンパクトな構成に収めて得られるので好ましい。[0008] As the semi-permeable membrane of the filter portion, a separation membrane usually called a microfiltration membrane, an ultrafiltration membrane, a reverse osmosis membrane or the like can be used, and the material is aromatic polyamide, allyl-alkyl polyamide / polyurea. , Polypiperazine amide, cellulose acetate, crosslinked cellulose, crosslinked polyether, sulfonated polysulfone, and the like. As the shape of the semipermeable membrane, a hollow fiber membrane is preferable because a large filtration area can be obtained in a compact configuration.
【０００９】以上のように試薬流出口側より加圧された
試薬は放出口より放出されるが、具体的には、例えば放
出口から放出路を介して排液部へ排出され、その放出路
には電磁開閉弁が介接される。なお、排液部は、計測部
から排出される排液（例えば試薬と試料液）を受け入れ
る排液部を兼ねるのが好ましい。以上のごとく、半透過
膜に付着した細菌等の粒子及び気泡を除くためには、実
際には、特定の制御部が用いられる。すなわち、その制
御部は、所定の設定時に、試薬流出口より試薬を加圧
し、加圧された試薬を放出口より放出すべく、加圧空気
源、３方切換弁、放出路の電磁開閉弁等に作動を指令
し、それによって自動的に半透過膜の付着物や気泡を除
かせる。この操作は、測定回数ごと、もしくは日数ごと
やシャットダウン時に自動的に行えるように設定できる
ことが好ましい。As described above, the reagent pressurized from the reagent outlet side is released from the discharge port. Specifically, for example, the reagent is discharged from the discharge port to the drain through a discharge path, and the discharge path is provided. , An electromagnetic on-off valve is interposed. It is preferable that the drain section also serves as a drain section that receives a drain liquid (for example, a reagent and a sample liquid) discharged from the measuring section. As described above, a specific control unit is actually used to remove particles such as bacteria and air bubbles attached to the semipermeable membrane. That is, the control unit pressurizes the reagent from the reagent outlet at a predetermined setting and discharges the pressurized reagent from the discharge port. , Etc., so as to automatically remove deposits and air bubbles on the semi-permeable membrane. It is preferable that this operation can be set so that it can be performed automatically every measurement count, every day, or at the time of shutdown.
【００１０】このシャットダウンとは、機器の使用が終
了し電源を切る際に機器が自動的に行なう洗浄等の動作
を意味する。[0010] The shutdown means an operation such as cleaning automatically performed by the device when the use of the device is finished and the power is turned off.
【００１１】[0011]
【発明の実施の形態】以下、図面に示す実施の形態に基
づいてこの発明を詳述する。なお、これによってこの発
明が限定されるものではない。図１は、この発明の１つ
の実施の形態を示す全体構成説明図である。図１におい
て、粒子計数装置としてのフローサイトメータ１は、試
料液（蛍光染色された尿）中の粒子（例えば細菌）を計
測しようとするもので、試薬容器２と、試薬加圧部とし
ての試薬加圧チャンバ３と、フィルター部（除菌フィル
ター）４と、試薬ヒータ５と、計測部としてのフローセ
ル６と、排液部としての排液チャンバ７と、制御部とし
ての制御回路４０とから主としてなる。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail based on an embodiment shown in the drawings. Note that the present invention is not limited to this. FIG. 1 is an explanatory diagram of the overall configuration showing one embodiment of the present invention. In FIG. 1, a flow cytometer 1 as a particle counting device is to measure particles (for example, bacteria) in a sample solution (fluorescent stained urine), and includes a reagent container 2 and a reagent pressurizing unit. A reagent pressurizing chamber 3, a filter unit (sterilization filter) 4, a reagent heater 5, a flow cell 6 as a measuring unit, a drain chamber 7 as a drain unit, and a control circuit 40 as a control unit. Be the main.
【００１２】フィルター部４は中心軸Ｘを垂直とする胴
部８と、上キャップ９及びそのカップリング１０と、下
キャップ１１及びそのカップリング１２と、半透過膜と
しての多数の中空系膜（ポリエチレン樹脂製）の群１３
と、この中空系膜の群を胴部８の上部にシールし固着す
るシール部材１４と、胴部８から外方へ延びる放出部１
６とからなる。The filter portion 4 includes a body portion 8 having a central axis X perpendicular to the upper portion, an upper cap 9 and a coupling 10 thereof, a lower cap 11 and a coupling 12 thereof, and a plurality of hollow membranes as semi-permeable membranes. Group 13 of polyethylene resin)
A sealing member 14 for sealing and fixing the group of hollow membranes to the upper portion of the body 8, and a discharge portion 1 extending outward from the body 8.
6
【００１３】ここで中空系膜の群１３は、各中空系膜
（例えば１３ａ）が胴部８内でＵ字状に屈曲されてその
両端部をシール部材１４によってシールされ、中空糸膜
の外側が高圧側、内側が低圧側となるよう区画形成され
ている。そして胴部８の高圧側には電磁開閉弁１７を介
して試薬加圧チャンバ３が、低圧側には試薬ヒータ５が
それぞれ連結されている。Here, the group 13 of hollow membranes is such that each hollow membrane (for example, 13a) is bent in a U-shape in the body 8 and both ends thereof are sealed by seal members 14, and the outside of the hollow fiber membrane is formed. Are formed on the high pressure side and the inside is on the low pressure side. The reagent pressurizing chamber 3 is connected to the high-pressure side of the body 8 via an electromagnetic on-off valve 17, and the reagent heater 5 is connected to the low-pressure side.
【００１４】放出部１６は、胴部８の高圧側の上方部分
の放出口１６ａから排液チャンバ７へ延びる放出路１８
と、この放出路に介接された電磁開閉弁１９とからな
る。試薬ヒータ５は、試薬の加温室２０とこの加温室に
一体に胴巻きにされた面ヒータ（図示省略）とからな
り、加温室２０の入口はフィルター部４の低圧側に連結
され、加温室２０の出口は３方切換弁２２を介して後述
するフローセル６の試薬入口２４に流路１５で連結され
ている。３方切換弁２２のもう１方のポートは電磁切換
弁２３を介して試薬逆加圧部としての加圧空気源２５に
連結されている。The discharge section 16 is provided with a discharge path 18 extending from the discharge port 16a in the upper portion of the body section 8 on the high pressure side to the drain chamber 7.
And an electromagnetic on-off valve 19 interposed in the discharge path. The reagent heater 5 includes a reagent heating chamber 20 and a surface heater (not shown) integrally wound around the heating chamber. An inlet of the heating chamber 20 is connected to the low-pressure side of the filter unit 4. Is connected via a three-way switching valve 22 to a reagent inlet 24 of the flow cell 6 described later via a flow path 15. The other port of the three-way switching valve 22 is connected via an electromagnetic switching valve 23 to a pressurized air source 25 as a reagent reverse pressurizing unit.
【００１５】フローセル６は、試薬導入口２４と試料液
導入口２６を有し、試料液導入口２６から導入された試
料液を試薬導入口２４から導入された試薬で包むように
細い流れに収束させるフローセル本体２７と、試料液導
入口２６に連結されたサンプルシリンジ２８と、上述の
収束された細い流れにレーザ光を照射しその散乱光の蛍
光とを検出する検知器２９とからなり、このフローセル
本体２７から流れ出た試薬及び試料液は電磁開閉弁３０
を介して排液チャンバ７に至る。なお、３１は排液チャ
ンバ７の排出口に設けられた電磁開閉弁、３２は試薬ボ
トル２と試薬加圧チャンバ３との間に介接された逆流防
止弁、３３は同じく電磁開閉弁、３４は３方切換弁２２
と試薬導入口２４との間に介接された逆流防止弁であ
る。The flow cell 6 has a reagent inlet 24 and a sample liquid inlet 26, and converges the sample liquid introduced from the sample liquid inlet 26 into a thin flow so as to be wrapped by the reagent introduced from the reagent inlet 24. The flow cell comprises a flow cell main body 27, a sample syringe 28 connected to the sample liquid inlet 26, and a detector 29 for irradiating the converged narrow flow with laser light and detecting the fluorescence of the scattered light. The reagent and the sample solution flowing out of the main body 27 are
Through the drainage chamber 7. Numeral 31 denotes an electromagnetic on-off valve provided at the outlet of the drain chamber 7, 32 denotes a check valve interposed between the reagent bottle 2 and the reagent pressurizing chamber 3, 33 denotes an electromagnetic on-off valve, 34 Is a three-way switching valve 22
And a check valve interposed between the reagent inlet 24 and the reagent introduction port 24.
【００１６】次に、以上の構成からなるフローサイトメ
ータ１の作動を説明する。まず試薬ボトル２から逆流防
止弁３２及び電磁開閉弁３３を介して試薬加圧チャンバ
３に導入された試薬は、加圧された空気（約０．２kg／
cm2）の供給によって加圧され、電磁開閉弁１７を介し
てフィルター部４の中空糸膜の群１３の高圧側へ供給さ
れる。そして中空系膜の群１３を介して不純物（細菌、
ガスなど）が除去され、次いで試薬は試薬ヒータ５を介
して約３５℃に加温され（温度調節され）、さらに３方
切換弁２２を介してフローセル６の試薬導入口２４へ供
給される。Next, the operation of the flow cytometer 1 having the above configuration will be described. First, the reagent introduced from the reagent bottle 2 into the reagent pressurizing chamber 3 via the check valve 32 and the solenoid on-off valve 33 is compressed air (about 0.2 kg /
cm 2 ), and is supplied to the high pressure side of the hollow fiber membrane group 13 of the filter unit 4 via the electromagnetic on-off valve 17. And impurities (bacteria,
Gas and the like are removed, and then the reagent is heated to about 35 ° C. (temperature controlled) via the reagent heater 5 and further supplied to the reagent inlet 24 of the flow cell 6 via the three-way switching valve 22.
【００１７】一方、試料液がサンプルシリンジ２８から
供給され、試料液が試薬で包まれるようにして細い流れ
に収束させながら試料液中を流れる粒子（細菌）が検知
器２９で計測される。すなわち、上述の細い流れにレー
ザ光（例えばアルゴンレーザ光）が照射され、尿中有形
成分に当たった際に生じる前方散乱光と蛍光とがフォト
ダイオードとフォトマルチプライヤ（いずれも図示概
略）で検出され、適宜画像処理されて細菌が分画計測さ
れる。そして計測を終えフローセル６を流れ出た試薬と
試料液は電磁開閉弁３０を介して排液チャンバ７へ排出
される。On the other hand, the sample liquid is supplied from the sample syringe 28, and particles (bacteria) flowing in the sample liquid are measured by the detector 29 while converging the sample liquid in a narrow flow so as to be covered with the reagent. That is, laser light (for example, argon laser light) is applied to the above-mentioned narrow flow, and forward scattered light and fluorescent light generated when the fine flow is applied to the formed components in urine are converted by a photodiode and a photomultiplier (both shown schematically). The detected bacteria are subjected to appropriate image processing, and the bacteria are fractionated and measured. After the measurement, the reagent and the sample liquid flowing out of the flow cell 6 are discharged to the drain chamber 7 via the electromagnetic on-off valve 30.
【００１８】そしてフローサイトメータ１による計測が
終了した段階で、電磁開閉弁１９を開に、そして３方切
換弁２２を切換えて、加圧空気源２５を作動させ（約
０．５kg／cm2）、フィルター部４の中空糸膜の群１３
で仕切られた低圧側の試薬を逆加圧し中空糸膜の群１３
の目詰まり粒子を高圧側へ剥離させると共に剥離された
粒子及びその付近に浮遊するガスを放出路１８を介して
排液チャンバ７へ排出する。このように中空糸膜の群１
３の目詰まり粒子を剥離・除去することによって、フィ
ルター部４の寿命を延ばすことができる。また浮遊ガス
が除去できることで、試薬加圧チャンバ３からの圧力が
浮遊ガスで吸収されることなく試薬に伝達され、フロー
セル６内での試薬の流速を安定させ（低下を防止し）高
精度の計測を可能にする。なお、このような目詰まり粒
子の剥離及び放出は計測終了時ごとだけではなく、途中
で定期的に、又は計測前に行うことができる。また加圧
空気源２５を作動させることなく、単に放出路１８の電
磁開閉弁１９を一定時間開にして中空糸膜の群１３の高
圧側の面付近に浮遊する細菌やガスを放出除去してもよ
い。When the measurement by the flow cytometer 1 is completed, the solenoid on-off valve 19 is opened and the three-way switching valve 22 is switched to operate the pressurized air source 25 (about 0.5 kg / cm 2). ), Group 13 of hollow fiber membranes in filter section 4
Group 13 of the hollow fiber membrane
The clogged particles are peeled to the high pressure side, and the peeled particles and the gas floating near the particles are discharged to the drainage chamber 7 through the discharge path 18. Thus, the hollow fiber membrane group 1
The life of the filter unit 4 can be extended by peeling and removing the clogged particles 3. In addition, since the floating gas can be removed, the pressure from the reagent pressurizing chamber 3 is transmitted to the reagent without being absorbed by the floating gas, and the flow rate of the reagent in the flow cell 6 is stabilized (prevented from dropping) and highly accurate. Enable measurement. In addition, such peeling and release of the clogged particles can be performed not only at the end of the measurement but also periodically or before the measurement. Further, without operating the pressurized air source 25, the electromagnetic on / off valve 19 of the discharge path 18 is simply opened for a certain period of time to release and remove bacteria and gas floating near the surface on the high pressure side of the group 13 of hollow fiber membranes. Is also good.
【００１９】なお、中空糸膜（又は中空糸）としては、
材質はオレフィン系の樹脂、たとえば上述のごとくポリ
エチレン樹脂が挙げられるが、一般的に孔径は細菌を除
去するためには、０．３μｍ以下が必要で、望ましくは
０．１μｍ、フィルターの有効表面積としては３０００
〜５０００cm程度が適当である。フィルターの寸法とし
ては、直径に比べ長さが２倍以上で長いほうが好まし
く、例えば直径４０mmで、長さ１２０mmのものが挙げら
れる。The hollow fiber membrane (or hollow fiber) includes
The material is an olefin-based resin, for example, a polyethylene resin as described above. Generally, the pore size is required to be 0.3 μm or less, and preferably 0.1 μm, as the effective surface area of the filter, in order to remove bacteria. Is 3000
About 5000 cm is appropriate. As the dimensions of the filter, it is preferable that the length is at least twice as long as the diameter, and the filter is longer, for example, a filter having a diameter of 40 mm and a length of 120 mm.
【００２０】これはフィルターの有効面積が同じであれ
ば、長寸法の中空糸膜を使用し且つ中空糸膜の本数が少
なくなるほど、フィルターの目詰まりを防げて、寿命が
長くなる。（流量の初期変化率が低減できる。）If the effective area of the filter is the same, the longer the hollow fiber membrane is used and the number of the hollow fiber membranes is reduced, the more the filter can be prevented from being clogged, and the longer the life is. (The initial change rate of the flow rate can be reduced.)
【００２１】[0021]
【発明の効果】この発明によれば、試薬をフィルター部
で濾過するときとは逆に、フィルター部の試薬流出口よ
り試薬を加圧し、それによって半透過膜の試薬流入側面
に付着した細菌等の粒子や気泡を放出口より除き、半透
過膜の濾過効果の低下を遅らせてフィルター部の寿命を
延ばすことができる。According to the present invention, in contrast to the case where the reagent is filtered through the filter, the reagent is pressurized from the reagent outlet of the filter, thereby causing bacteria and the like adhering to the reagent inflow side of the semipermeable membrane. By removing particles and air bubbles from the outlet, the reduction of the filtration effect of the semi-permeable membrane can be delayed to extend the life of the filter section.
【図１】この発明の１つの実施の形態を示す全体構成説
明図である。FIG. 1 is an explanatory diagram of an overall configuration showing one embodiment of the present invention.
１ フローサイトメータ ２ 試薬ボトル ３ 試薬加圧チャンバ ４ フィルター部 ５ 試薬ヒータ ６ フローセル ７ 排液チャンバ ８ 胴部 ９ 上キャップ １０ カップリング １１ 下キャップ １２ カップリング １３ 中空系膜の群 １４ シール部材 １５ 流路 １６ 放出部 １７ 電磁開閉弁 １８ 放出路 １９ 電磁開閉弁 ２０ 加温室 ２２ ３方切換弁 ２３ 電磁切換弁 ２４ 試薬導入口 ２５ 加圧空気源 ２６ 試料液導入口 ２７ フローセル本体 ２８ サンプルシリンジ ２９ 検知器 ３０、３１ 電磁開閉弁 ３２ 逆流防止弁 ３３ 電磁開閉弁 ３４ 逆流防止弁 DESCRIPTION OF SYMBOLS 1 Flow cytometer 2 Reagent bottle 3 Reagent pressurization chamber 4 Filter part 5 Reagent heater 6 Flow cell 7 Drainage chamber 8 Body 9 Upper cap 10 Coupling 11 Lower cap 12 Coupling 13 Group of hollow membranes 14 Seal member 15 Flow Path 16 discharge section 17 electromagnetic on-off valve 18 discharge path 19 electromagnetic on-off valve 20 heating chamber 22 three-way switching valve 23 electromagnetic switching valve 24 reagent inlet 25 pressurized air source 26 sample liquid inlet 27 flow cell body 28 sample syringe 29 detector 30, 31 solenoid on-off valve 32 check valve 33 solenoid on-off valve 34 check valve
Claims (5)
流出口付近に放出口を備えたフィルター部を、試薬容器
と計測部との間に備えた粒子計数装置において、 試薬流出口より試薬を加圧し、加圧された試薬を放出口
より放出することによって、半透過膜に付着した細菌等
の粒子および気泡を除くよう構成されたことを特徴とす
る粒子計数装置。1. A particle counting apparatus comprising a filter section provided with a discharge port near a reagent outlet on the reagent inflow side partitioned by a semi-permeable membrane between a reagent container and a measuring section. A particle counting device configured to pressurize a reagent and discharge the pressurized reagent from a discharge port to remove particles such as bacteria and air bubbles attached to the semipermeable membrane.
ルター部と試薬容器との間に逆流防止弁を備えた請求項
１記載の粒子計数装置。2. The particle counter according to claim 1, further comprising a check valve between the filter unit and the measuring unit and between the filter unit and the reagent container.
に半透過膜に付着した細菌等の粒子および気泡を除く作
動をすることを特徴とする請求項３記載の粒子計数装
置。3. The particle counting device according to claim 3, wherein the device automatically removes particles such as bacteria and air bubbles attached to the semi-permeable membrane every number of times of measurement or every number of days.
付着した細菌等の粒子および気泡を除く作動をすること
を特徴とする請求項３記載の粒子計数装置。4. The particle counting apparatus according to claim 3, wherein the operation of automatically removing particles such as bacteria and air bubbles attached to the semi-permeable membrane at the time of shutdown is performed.
４のいずれかに記載の粒子計数装置。5. The semi-permeable membrane is a hollow fiber membrane.
5. The particle counting device according to any one of 4.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9137137A JPH10332568A (en) | 1997-05-27 | 1997-05-27 | Particle counting apparatus |
| US09/084,418 US6183697B1 (en) | 1997-05-27 | 1998-05-27 | Particle measuring apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9137137A JPH10332568A (en) | 1997-05-27 | 1997-05-27 | Particle counting apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH10332568A true JPH10332568A (en) | 1998-12-18 |
Family
ID=15191694
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9137137A Pending JPH10332568A (en) | 1997-05-27 | 1997-05-27 | Particle counting apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH10332568A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004029028A (en) * | 2003-07-11 | 2004-01-29 | Kunimune:Kk | Collection/preservation method for urine specimen |
| JP2009533675A (en) * | 2006-04-14 | 2009-09-17 | ホ シン，ユン | Ultrafiltration system for online analyzer |
| CN110595823A (en) * | 2019-10-25 | 2019-12-20 | 扬州大学 | Underwater sampling device for detecting ethylene in rice body and using method thereof |
-
1997
- 1997-05-27 JP JP9137137A patent/JPH10332568A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004029028A (en) * | 2003-07-11 | 2004-01-29 | Kunimune:Kk | Collection/preservation method for urine specimen |
| JP2009533675A (en) * | 2006-04-14 | 2009-09-17 | ホ シン，ユン | Ultrafiltration system for online analyzer |
| CN110595823A (en) * | 2019-10-25 | 2019-12-20 | 扬州大学 | Underwater sampling device for detecting ethylene in rice body and using method thereof |
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