JPH10291935A - Active oxygen free radical quencher and preventive for disease caused by active oxygen free radical - Google Patents
Active oxygen free radical quencher and preventive for disease caused by active oxygen free radicalInfo
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- JPH10291935A JPH10291935A JP9104403A JP10440397A JPH10291935A JP H10291935 A JPH10291935 A JP H10291935A JP 9104403 A JP9104403 A JP 9104403A JP 10440397 A JP10440397 A JP 10440397A JP H10291935 A JPH10291935 A JP H10291935A
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- organic solvent
- hot water
- active oxygen
- oxygen free
- free radical
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Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、生体内の代謝系等
で発生する活性酸素フリーラジカルを低減し、炎症、老
化、発癌、心筋梗塞などの活性酸素フリーラジカルに起
因する様々な疾患の予防に寄与することができる活性酸
素フリーラジカル消去剤及び活性酸素フリーラジカル起
因疾患予防剤に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention reduces active oxygen free radicals generated in the metabolic system in a living body and prevents various diseases caused by active oxygen free radicals such as inflammation, aging, carcinogenesis, and myocardial infarction. The present invention relates to an active oxygen free radical scavenger and an active oxygen free radical-induced disease preventive agent, which can contribute to the following.
【０００２】[0002]
【従来の技術及び発明が解決しようとする課題】スーパ
ーオキサイドアニオン（・Ｏ2 - ）、ヒドロシキラジカ
ル（・ＯＨ）、過酸化水素などの活性酸素フリーラジカ
ルは、生体内で多量に発生すると組織傷害を起こし、炎
症、老化、発癌、心筋梗塞など様々な疾患を起こすと言
われている。これに対し、生体は各種活性酸素フリーラ
ジカルを低減するためのいくつかの酵素を備えている。
例えば、スーパーオキサイドディスムターゼ（ＳＯＤ）
は、スーパーオキサイドアニオンを不均化反応で過酸化
水素に変換し、カタラーゼやグルタチオンペルオキシダ
ーゼは、過酸化水素を低減するといわれている。しかし
ながら、生体の酵素だけでは十分に活性酸素フリーラジ
カルの効力を低減できない場合がある。そこで、活性酸
素フリーラジカルに起因する様々な疾患を防ぐために、
活性酸素フリーラジカルを確実に低減し得る物質が求め
られる。BACKGROUND OF INVENTION Problems to be Solved] Superoxide anion (· O 2 -), hydroxyalkylcellulose radical (· OH), active oxygen free radicals such as hydrogen peroxide, a large quantity generated in the living tissue It is said to cause injury and various diseases such as inflammation, aging, carcinogenesis and myocardial infarction. In contrast, living organisms have several enzymes for reducing various active oxygen free radicals.
For example, superoxide dismutase (SOD)
Is said to convert superoxide anion into hydrogen peroxide by a disproportionation reaction, and it is said that catalase and glutathione peroxidase reduce hydrogen peroxide. However, there are cases in which the effectiveness of active oxygen free radicals cannot be sufficiently reduced only by living body enzymes. Therefore, in order to prevent various diseases caused by active oxygen free radicals,
There is a need for a substance that can reliably reduce active oxygen free radicals.
【０００３】また、一般的意識として合成品の使用につ
いては安全性の面から厳しい批判があるため、天然物由
来で安全性の高い活性酸素フリーラジカル消失剤が求め
られる。[0003] Further, there is severe criticism from the viewpoint of safety regarding the use of synthetic products as a general consciousness. Therefore, a safe and highly reactive active oxygen free radical scavenger derived from natural products is required.
【０００４】そこで本発明は、人体に対する安全性と経
済性の観点から、入手が容易で安価な天然物に由来し、
しかも強い活性酸素フリーラジカル消去活性を有する新
たな活性酸素フリーラジカル消失剤及び活性酸素フリー
ラジカル起因疾患予防剤を提供せんとする。Accordingly, the present invention is based on natural products that are easily available and inexpensive from the viewpoint of safety and economic efficiency for the human body.
In addition, a new active oxygen free radical scavenger having a strong active oxygen free radical scavenging activity and a preventive agent for a disease caused by active oxygen free radicals are provided.
【０００５】[0005]
【課題を解決するための手段】本発明者らは、かかる解
決課題のため各種植物及び食品について分析を行った結
果、バナバ葉（Lagerstroemia speciosa L. ）の抽出物
に強い活性酸素フリーラジカル消去作用があることを見
出し、これに基づいて活性酸素フリーラジカル消去剤及
び活性酸素フリーラジカル起因疾患予防剤を発明した。Means for Solving the Problems The present inventors have analyzed various plants and foods for the purpose of solving the problems and found that the extract of banaba leaves (Lagerstroemia speciosa L.) has a strong active oxygen free radical scavenging effect. Based on this finding, based on this, an active oxygen free radical scavenger and an active oxygen free radical-induced disease preventive agent were invented.
【０００６】本発明の活性酸素フリーラジカル消去剤及
び活性酸素フリーラジカル起因疾患予防剤は、バナバ葉
を熱水、有機溶媒、又は有機溶媒と熱水との混合溶液で
抽出して得られた粗抽出物を有効成分とするものであ
り、好ましくは当該粗抽出物を更にスチレン−ジビニル
ベンゼン系合成樹脂又はデキストラン系合成樹脂に吸着
させて得られる樹脂吸着成分を有効成分とするものであ
る。The active oxygen free radical scavenger and the active oxygen free radical-causing disease preventive agent of the present invention are obtained by extracting banaba leaves with hot water, an organic solvent, or a mixed solution of an organic solvent and hot water. The extract is an active ingredient, preferably a resin-adsorbed component obtained by further adsorbing the crude extract on a styrene-divinylbenzene-based synthetic resin or a dextran-based synthetic resin.
【０００７】本発明において活性酸素フリーラジカルと
は、体内で発生する活性酸素フリーラジカルとしてのス
ーパーオキサイドアニオン（・Ｏ2 - ）、ヒドロシキラ
ジカル（・ＯＨ）、過酸化水素等、及び安定なフリーラ
ジカルを有する１，１−ジフェニル−２−ピクリルヒド
ラジル（ＤＰＰＨ）等を含む意味である。また、本発明
における活性酸素起因疾患とは、活性酸素に起因する疾
患、すなわち歯肉炎、歯周炎等の歯周病、肺炎、老化、
イニシエーターやプロモーターに起因する肺癌及び胃
癌、動脈硬化に起因する心筋梗塞等の循環器疾患、並び
にベータアミロイドタンパクの毒性によるアルツハイマ
ー型痴呆症などの疾患を含む意味である。[0007] The active oxygen free radicals in the present invention, superoxide anion as the active oxygen free radicals generated in the body (· O 2 -), hydroxyalkylcellulose radical (· OH), hydrogen peroxide, and a stable free It is meant to include 1,1-diphenyl-2-picrylhydrazyl (DPPH) having a radical. The active oxygen-induced disease in the present invention is a disease caused by active oxygen, namely, gingivitis, periodontal disease such as periodontitis, pneumonia, aging,
The term is meant to include cardiovascular diseases such as lung cancer and gastric cancer caused by initiators and promoters, myocardial infarction caused by arteriosclerosis, and diseases such as Alzheimer's dementia caused by beta-amyloid protein toxicity.
【０００８】本発明の活性酸素フリーラジカル消去剤及
び活性酸素フリーラジカル起因疾患予防剤において、粗
抽出物又は樹脂吸着成分の含有量０．００１〜８０％が
好ましい。[0008] In the active oxygen free radical scavenger and the active oxygen free radical induced disease preventive agent of the present invention, the content of the crude extract or the resin-adsorbed component is preferably 0.001 to 80%.
【０００９】本発明で使用するバナバ葉は、バナバ生葉
を自然乃至強制的に十分乾燥させ、粉砕するのが好まし
い。バナバ葉を抽出する有機溶媒には、メタノール、エ
タノール、アセトン等の水溶性有機溶媒を使用すること
ができる。バナバ葉を抽出する熱水、有機溶媒又は有機
溶媒と熱水との混合溶液の量は、乾燥させたバナバ葉重
量に対し１：１０〜１：１００、好ましくは１：２５程
度とする。The banaba leaf used in the present invention is preferably obtained by naturally or compulsorily drying and pulverizing fresh banaba leaves. As an organic solvent for extracting banaba leaves, a water-soluble organic solvent such as methanol, ethanol, or acetone can be used. The amount of hot water, an organic solvent or a mixed solution of an organic solvent and hot water for extracting banaba leaves is 1:10 to 1: 100, preferably about 1:25, based on the weight of the dried banaba leaves.
【００１０】スチレン−ジビニルベンゼン系合成樹脂に
は、例えば三菱化成工業株式会社製ダイアイオンHP10、
20、30、40、50、オルガノ株式会社製アンバーライト X
AD-2、4 、或いは住友化学工業株式会社製デュオライト
Ｓシリーズ等を使用することができる。デキストラン系
合成樹脂には、例えばファルマシア社製Sephadex LH-20
等を使用することができる。Styrene-divinylbenzene synthetic resins include, for example, Diaion HP10 manufactured by Mitsubishi Kasei Kogyo Co., Ltd.
20, 30, 40, 50, Amberlite X made by Organo Corporation
AD-2, 4, or Duolite S series manufactured by Sumitomo Chemical Co., Ltd. can be used. Dextran synthetic resins include, for example, Sephadex LH-20 manufactured by Pharmacia
Etc. can be used.
【００１１】本発明の活性酸素フリーラジカル消去剤及
び活性酸素フリーラジカル起因疾患予防剤は、医薬品、
医薬部外品（化粧品、洗口剤などを含む）、健康食品、
健康飲料などとして様々に提供することができる。その
剤型は、凍結乾燥或いは噴霧乾燥などにより乾燥させて
乾燥粉末として提供することも、さらに液剤、錠剤、散
剤、顆粒、糖衣錠、カプセル、懸濁液、乳剤、アンプ
ル、注射剤などとして提供することもできる。医薬部外
品として調製し、これを缶ドリンク飲料、瓶ドリンク飲
料などの飲料形態、或いはタブレット、カプセル、顆粒
などの形態として日常的に容易に摂取することより生体
に対して十分な薬理効果をもたらす医薬部外品として提
供することもできる。The active oxygen free radical scavenger and the active oxygen free radical induced disease preventive agent of the present invention can be
Quasi-drugs (including cosmetics, mouthwashes, etc.), health foods,
It can be provided variously as a health drink or the like. The dosage form may be dried by freeze-drying or spray-drying to provide a dry powder, or may be further provided as a liquid, tablet, powder, granule, dragee, capsule, suspension, emulsion, ampoule, injection, or the like. You can also. Prepared as a quasi-drug, and obtained a pharmacological effect sufficient for the living body by easily ingesting it daily in the form of drinks such as canned drink drinks and bottle drink drinks, or tablets, capsules, granules, etc. It can also be provided as a quasi drug.
【００１２】また、本発明における粗抽出物及び樹脂吸
着成分は、単独でも有効であるが、ビタミンＡ（カロチ
ノイド類）、ビタミンＣ、ビタミンＥ、グルタチオン、
有機酸、アミノ酸、コウジ酸、茶エキスなどを配合すれ
ば一層効果を高めることができる。特に医薬品又は医薬
部外品として利用する場合には、ビタミンＡ（カロチノ
イド類）、ビタミンＣ、ビタミンＥ、グルタチオンを配
合するのが好ましい。Although the crude extract and the resin-adsorbing component in the present invention are effective even when used alone, vitamin A (carotenoids), vitamin C, vitamin E, glutathione,
If an organic acid, an amino acid, kojic acid, tea extract, etc. are blended, the effect can be further enhanced. In particular, when used as pharmaceuticals or quasi-drugs, it is preferable to mix vitamin A (carotenoids), vitamin C, vitamin E, and glutathione.
【００１３】[0013]
（活性酸素フリーラジカル消去剤及び活性酸素フリーラ
ジカル起因疾患予防剤の有効成分の製造）フィリピン産
バナバ生葉を適宜量強制的に十分乾燥させ、これを粉砕
しよく混合したバナバ葉１ｋｇを５０リットルの蒸留水
で３０分間、９５〜１００℃で抽出した。この時の抽出
溶液のブリックス（Ｂｒｉｘ）は約１．５％であった。
得られた抽出液をろ過、遠心分離した後、更にエバポレ
ーターで濃縮し、噴霧乾燥等の方法により乾燥させてバ
ナバ葉粗抽出物（以下「粗抽出物」という。）を得た。
この時、粗抽出物の収率は、乾燥バナバ葉（原料）に対
して約１０重量％であった。(Manufacture of active ingredients for active oxygen free radical scavenger and active oxygen free radical-induced disease preventive agent) Fresh banaba leaves from Philippines are forcibly and thoroughly dried in an appropriate amount, pulverized and mixed well, and 1 kg of banaba leaves is mixed with 50 liters. Extracted with distilled water for 30 minutes at 95-100 ° C. At this time, Brix of the extraction solution was about 1.5%.
After the obtained extract was filtered and centrifuged, the extract was further concentrated by an evaporator and dried by a method such as spray drying to obtain a crude extract of banaba leaf (hereinafter referred to as “crude extract”).
At this time, the yield of the crude extract was about 10% by weight based on the dried banaba leaves (raw material).
【００１４】次に、直径１０ｃｍ、長さ１００ｃｍのガ
ラスカラムにスチレン−ジビニルベンゼン系合成樹脂
（三菱化成工業株式会社製ダイアイオンHP20）を２．５
リットル充填し、この充填材を先ずメタノールで洗浄後
さらに蒸留水で洗浄し、その後、上記粗抽出物（全量）
を１リットルの蒸留水に溶かしてこれをガラスカラム内
に流した。次に、カラム容量に対して約６倍量相当の蒸
留水を流してスチレン−ジビニルベンゼン系合成樹脂に
吸着されなかった分画（以下「非吸着分画」という。）
を溶出させた後、カラム容量に対して約５倍量相当の１
００％メタノールを流してスチレン−ジビニルベンゼン
系合成樹脂に吸着された分画（以下「吸着分画」とい
う。）を溶出させた。Next, styrene-divinylbenzene synthetic resin (Diaion HP20 manufactured by Mitsubishi Kasei Kogyo Co., Ltd.) was added to a glass column having a diameter of 10 cm and a length of 100 cm.
1 liter, and the filler is washed with methanol and then with distilled water, and then the above crude extract (total amount)
Was dissolved in 1 liter of distilled water, and this was flown into a glass column. Next, a fraction that was not adsorbed to the styrene-divinylbenzene-based synthetic resin by flowing distilled water equivalent to about 6 times the column capacity (hereinafter referred to as “non-adsorbed fraction”).
After elution, 1 equivalent of about 5 times the column volume
The fraction adsorbed on the styrene-divinylbenzene-based synthetic resin was eluted by flowing 00% methanol (hereinafter referred to as "adsorbed fraction").
【００１５】こうして得られた吸着分画及び非吸着分画
をそれぞれ濃縮し、凍結乾燥して乾燥粉末とした。この
時の収率は、乾燥バナバ葉（原料）に対して、吸着分画
約２．７重量％、非吸着分画約４．３重量％であった。The thus obtained adsorbed fraction and non-adsorbed fraction were respectively concentrated and freeze-dried to obtain a dry powder. The yield at this time was about 2.7% by weight of the adsorbed fraction and about 4.3% by weight of the non-adsorbed fraction based on the dried banaba leaf (raw material).
【００１６】（１，１−ジフェニル−２−ピクリルヒド
ラジルの消去活性の測定）本試験では、バナバ葉抽出物
について安定フリーラジカルとして知られる１，１−ジ
フェニル−２−ピクリルヒドラジル（ＤＰＰＨ）の消去
活性を測定した。(Measurement of Scavenging Activity of 1,1-Diphenyl-2-picrylhydrazyl) In this test, 1,1-diphenyl-2-picrylhydrazyl (known as a stable free radical) on banaba leaf extract was used. The scavenging activity of DPPH) was measured.
【００１７】（試薬の調製）ＤＰＰＨは、和光純薬工業
製を使用し、その他の試薬は半井テスク製を使用した。(Preparation of Reagents) DPPH was manufactured by Wako Pure Chemical Industries, and other reagents were manufactured by Hanui Tesque.
【００１８】（測定方法）予め試験管内において、０．
５ｍＭのＤＰＰＨフリーラジカル／エタノール溶液０．
５ｍｌ、０．１Ｍの酢酸緩衝液（ｐＨ５．５）０．９ｍ
ｌ及びエタノール１．０ｍｌを混合しておき、この試験
管に０．１Ｍの酢酸緩衝液（ｐＨ５．５）によって各種
濃度に調製した粗抽出物、吸着分画又は非吸着分画を
０．１ｍｌ加え、３０分室温で放置した後、５１７ｎｍ
における吸光度を測定した。また、比較例としてビタミ
ンＥ（α−トコフェロール）についても同様にして吸光
度を測定した。(Measurement method) In a test tube, 0.1%
5 mM DPPH free radical / ethanol solution
5 ml, 0.1 M acetate buffer (pH 5.5) 0.9 m
1 ml of ethanol and 1.0 ml of ethanol, and 0.1 ml of a crude extract, adsorbed fraction or non-adsorbed fraction adjusted to various concentrations with 0.1 M acetate buffer (pH 5.5) was added to the test tube. In addition, after standing at room temperature for 30 minutes, 517 nm
Was measured. As a comparative example, the absorbance of vitamin E (α-tocopherol) was measured in the same manner.
【００１９】（結果及び考察）図１は、粗抽出物、吸着
分画、非吸着分画及びビタミンＥのＤＰＰＨフリーラジ
カル消去活性を示したものである。ＤＰＰＨフリーラジ
カルは５１７ｎｍ付近に吸光度を持ち、それが低減され
るとその吸光度が減少する。図１の結果より、粗抽出
物、吸着分画及び非吸着分画はいずれも、濃度依存的に
ＤＰＰＨフリーラジカルの吸光度を減少させ、特に粗抽
出物及び吸着分画に強い効果が観察された。また、この
効果はビタミンＥと比較してもより強い効果であった。
従って、粗抽出物及び吸着分画はいずれも、ＤＰＰＨフ
リーラジカルなどの活性酸素フリーラジカル消去剤とし
て有効であり、活性酸素フリーラジカルに起因する様々
な疾患の予防剤として有効である。(Results and Discussion) FIG. 1 shows the crude extract, the adsorbed fraction, the non-adsorbed fraction, and the DPPH free radical scavenging activity of vitamin E. DPPH free radicals have an absorbance around 517 nm, and when it is reduced, the absorbance decreases. 1, the crude extract, the adsorbed fraction, and the non-adsorbed fraction all reduced the DPPH free radical absorbance in a concentration-dependent manner, and a strong effect was particularly observed on the crude extract and the adsorbed fraction. . This effect was stronger than that of vitamin E.
Therefore, both the crude extract and the adsorptive fraction are effective as scavengers for active oxygen free radicals such as DPPH free radicals, and are also effective as agents for preventing various diseases caused by active oxygen free radicals.
【００２０】（スーパーオキサイドアニオン（・
Ｏ2 - ）の消去活性の測定）本試験では、ヒポキサンチ
ン−キサンチンオキシダーゼ系によって発生させたスー
パーオキサイドアニオン（・Ｏ2 - ）をスピントラッピ
ング剤としてＤＭＰＯ（5,5-dimethyl-1-pyrroline-N-o
xide) を用い、スピンアダクトであるＤＭＰＯ−Ｏ2-を
測定した。(Superoxide anion (.
Measurement of elimination activity of O 2 − ) In this test, superoxide anion (· O 2 − ) generated by hypoxanthine-xanthine oxidase system was used as a spin trapping agent for DMPO (5,5-dimethyl-1-pyrroline-). No
xide) was used to measure DMPO-O 2- as a spin adduct.
【００２１】（試薬の調製）ＤＭＰＯは同仁ラボ社製を
使用し、ヒポキサンチンはシグマ社製を使用し、キサン
チンオキシダーゼはベーリンガー・マンハイム社を使用
し、ＳＯＤ（スーパーオキサイドヂスムターゼ）はフナ
コシ社製を使用した。以上の試料は、０．１Ｍリン酸緩
衝液（ｐＨ７．８）によって各種濃度に調製した。(Preparation of reagents) DMPO was manufactured by Dojin Lab, hypoxanthine was manufactured by Sigma, xanthine oxidase was manufactured by Boehringer Mannheim, and SOD (superoxide dismutase) was manufactured by Funakoshi. Was used. The above samples were prepared at various concentrations with a 0.1 M phosphate buffer (pH 7.8).
【００２２】（測定方法）予め試験管内において、ＤＭ
ＰＯ１５μｌと２ｍＭのヒポキサンチン５０μｌとを混
合しておき、この試験管に各種濃度に調製したＳＯＤ標
準溶液、粗抽出物、吸着分画又は非吸着分画を５０μｌ
加え、次いで０．３３ｕｎｉｔ／ｍｌのキサンチンオキ
シダーゼ溶液５０μｌを加えて攪拌した後、この試験管
から２００μｌの混合溶液を特殊偏平セルに吸い取り、
ＥＳＲ装置（日本電子製JES-FR80）を用いて４０秒後に
測定開始した。スペクトルの読みとりはＤＭＰＯ−０2-
のシグナル高さを内部標準物質として用いるマンガンの
シグナル高さと比較する方法で行った。(Measurement method) In a test tube, DM
15 μl of PO and 50 μl of 2 mM hypoxanthine were mixed, and 50 μl of SOD standard solution, crude extract, adsorbed fraction or non-adsorbed fraction prepared at various concentrations was added to the test tube.
Then, after adding 50 μl of a 0.33 unit / ml xanthine oxidase solution and stirring, 200 μl of the mixed solution was sucked from the test tube into a special flat cell,
The measurement was started after 40 seconds using an ESR device (JES-FR80 manufactured by JEOL Ltd.). The reading of the spectrum is DMPO-0 2-
Was performed by a method of comparing the signal height of manganese with the signal height of manganese used as an internal standard.
【００２３】（結果及び考察）図２は、粗抽出物、吸着
分画及び非吸着分画のスーパーオキサイドアニオンの消
去活性を示したものである。ヒポキサンチン／キサンチ
ンオキシダーゼ反応系から発生するスーパーオキサイド
アニオン（・Ｏ2 - ）は、スピントラップ剤であるＤＭ
ＰＯにトラップされるとＤＭＰＯ−Ｏ2-としてピークが
現れるが、ＳＯＤやスーパーオキサイドアニオン消去活
性を有する物質が存在するとＤＭＰＯ−Ｏ2-量が減少す
る。図２の結果より、粗抽出物、吸着分画及び非吸着分
画はいずれも、ＤＭＰＯ−Ｏ2-量を低減することが確か
められ、その低減能力は、能力の高いものから順に吸着
分画、粗抽出物、非吸着分画であることが分かった。従
って、粗抽出物及び吸着分画はいずれもスーパーオキサ
イドアニオンなどの活性酸素フリーラジカル消去剤とし
て有効であり、活性酸素フリーラジカルに起因する様々
な疾患の予防剤として有効である。(Results and Discussion) FIG. 2 shows the elimination activity of the superoxide anion in the crude extract, the adsorbed fraction and the non-adsorbed fraction. The superoxide anion (· O 2 − ) generated from the hypoxanthine / xanthine oxidase reaction system is converted to a spin trapping agent, DM.
When trapped by PO, a peak appears as DMPO-O 2- , but the presence of a substance having SOD or superoxide anion scavenging activity reduces the amount of DMPO-O 2- . From the results shown in FIG. 2, it was confirmed that the crude extract, the adsorbed fraction, and the non-adsorbed fraction all reduced the amount of DMPO-O 2− , and the decreasing ability was as follows. , A crude extract and a non-adsorbed fraction. Therefore, both the crude extract and the adsorptive fraction are effective as scavengers for active oxygen free radicals such as superoxide anion, and are effective as preventive agents for various diseases caused by active oxygen free radicals.
【００２４】（実施例１）粗抽出物（又は吸着分画）を
以下の割合で配合し、活性酸素フリーラジカル消去活性
効果のある化粧品又は医薬部外品の範疇に含まれる洗口
剤を作成した。 粗抽出物（又は吸着分画）・・・・ １．０ 重量％ パラオキシ安息香酸エステル ・・ ０．０５重量％ プロピレングリコール ・・・・・ １．０ 重量％ 濃グリセリン ・・・・・・・・ １．０ 重量％ クエン酸ナトリウム ・・・・・・ ０．０５重量％ 香料 ・・・・・・・・・・・・ １．９ 重量％ 精製水 ・・・・・・・・・・・ ９５．０ 重量％Example 1 A crude extract (or an adsorbed fraction) was blended at the following ratio to prepare a mouthwash contained in the category of cosmetics or quasi-drugs having an active oxygen free radical scavenging activity. did. Crude extract (or adsorptive fraction) ... 1.0% by weight paraoxybenzoate ... 0.05% by weight propylene glycol ... 1.0% by weight concentrated glycerin ... 1.0% by weight sodium citrate 0.05% by weight Perfume 1.9% by weight Purified water・ 95.0% by weight
【００２５】（実施例２）粗抽出物（又は吸着分画）を
以下の割合で配合し、活性酸素フリーラジカル消去活性
効果のある食品又は医薬品を作成した。 粗抽出物（又は吸着分画）・・・・・ ５０．０ ｍｇ ビタミンＣ ・・・・・・・・・・ ５０．０ ｍｇ 乳化オリゴ糖 ・・・・・・・・・ ９０．０ ｍｇ 造粒剤 ・・・・・・・・・・・・ ６０．０ ｍｇ 結晶セルロース ・・・・・・・・ ８０．０ ｍｇ 還元麦芽糖水飴 ・・・・・・・ ９０．０ ｍｇ スクロース ・・・・・・・・・・ １００．０ ｍｇ 香料 ・・・・・・・・・・・・ 適量Example 2 A crude extract (or an adsorbed fraction) was blended at the following ratio to prepare a food or drug having an active oxygen free radical scavenging activity. Crude extract (or adsorption fraction) 50.0 mg Vitamin C 50.0 mg Emulsified oligosaccharide 90.0 mg Granulating agent 60.0 mg crystalline cellulose 80.0 mg reduced maltose starch syrup 90.0 mg sucrose ······· 100.0 mg Fragrance ···
【図１】 バナバ葉の粗抽出物、吸着分画、非吸着分画
及びビタミンＥのＤＰＰＨフリーラジカルに対する消去
活性を示したグラフである。FIG. 1 is a graph showing the elimination activity of a banaba leaf crude extract, an adsorbed fraction, a non-adsorbed fraction and vitamin E on DPPH free radicals.
【図２】 バナバ葉の粗抽出物、吸着分画及び非吸着分
画のスーパーオキサイドアニオンに対する消去活性を示
したグラフである。FIG. 2 is a graph showing the scavenging activity of a crude extract of banaba leaves, an adsorbed fraction and a non-adsorbed fraction on superoxide anion.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 河野 雅弘 東京都昭島市武蔵野３−１−２ 日本電子 株式会社内 (72)発明者 角田 隆巳 静岡県榛原郡相良町女神21 株式会社伊藤 園内 ──────────────────────────────────────────────────続 き Continued on the front page (72) Masahiro Kono, Inventor 3-1-2 Musashino, Akishima-shi, Tokyo Japan Electronics Co., Ltd.
Claims (8)
媒と熱水との混合溶液で抽出して得られた粗抽出物を有
効成分とする活性酸素フリーラジカル消去剤。An active oxygen free radical scavenger comprising, as an active ingredient, a crude extract obtained by extracting banaba leaves with hot water, an organic solvent, or a mixed solution of an organic solvent and hot water.
媒と熱水との混合溶液で抽出して得られた粗抽出物中の
成分であって、スチレン−ジビニルベンゼン系合成樹脂
又はデキストラン系合成樹脂に吸着される樹脂吸着成分
を有効成分とする活性酸素フリーラジカル消去剤。2. A component in a crude extract obtained by extracting banaba leaves with hot water, an organic solvent, or a mixed solution of an organic solvent and hot water, wherein the styrene-divinylbenzene-based synthetic resin or dextran is used. An active oxygen free radical scavenger containing, as an active ingredient, a resin adsorption component adsorbed on a synthetic resin.
媒と熱水との混合溶液で抽出して得られた粗抽出物と、
ビタミンＣ乃至ビタミンＥとを混合して得られる混合物
を有効成分とする活性酸素フリーラジカル消去剤。3. A crude extract obtained by extracting banaba leaves with hot water, an organic solvent, or a mixed solution of an organic solvent and hot water,
An active oxygen free radical scavenger comprising, as an active ingredient, a mixture obtained by mixing vitamin C to vitamin E.
媒と熱水との混合溶液で抽出して得られた粗抽出物中の
成分であってスチレン−ジビニルベンゼン系合成樹脂又
はデキストラン系合成樹脂に吸着される樹脂吸着成分
と、ビタミンＣ乃至ビタミンＥとを混合して得られる混
合物を有効成分とする活性酸素フリーラジカル消去剤。4. A component in a crude extract obtained by extracting banaba leaves with hot water, an organic solvent, or a mixed solution of an organic solvent and hot water, and is a styrene-divinylbenzene synthetic resin or a dextran-based resin. An active oxygen free radical scavenger comprising, as an active ingredient, a mixture obtained by mixing a resin adsorption component adsorbed on a synthetic resin with vitamin C to vitamin E.
媒と熱水との混合溶液で抽出して得られた粗抽出物を有
効成分とする活性酸素フリーラジカル起因疾患予防剤。5. An agent for preventing a disease caused by active oxygen free radicals, comprising, as an active ingredient, a crude extract obtained by extracting banaba leaves with hot water, an organic solvent, or a mixed solution of an organic solvent and hot water.
媒と熱水との混合溶液で抽出して得られた粗抽出物中の
成分であって、スチレン−ジビニルベンゼン系合成樹脂
又はデキストラン系合成樹脂に吸着される樹脂吸着成分
を有効成分とする活性酸素フリーラジカル起因疾患予防
剤。6. A component in a crude extract obtained by extracting banaba leaves with hot water, an organic solvent, or a mixed solution of an organic solvent and hot water, wherein the styrene-divinylbenzene synthetic resin or dextran is used. An active oxygen free radical-induced disease preventive agent comprising a resin-adsorbed component adsorbed on a synthetic resin as an active ingredient.
媒と熱水との混合溶液で抽出して得られた粗抽出物と、
ビタミンＣ乃至ビタミンＥとを混合して得られる混合物
を有効成分とする活性酸素フリーラジカル起因疾患予防
剤。7. A crude extract obtained by extracting banaba leaves with hot water, an organic solvent, or a mixed solution of an organic solvent and hot water,
An active oxygen free radical-induced disease preventive agent comprising, as an active ingredient, a mixture obtained by mixing vitamin C to vitamin E.
媒と熱水との混合溶液で抽出して得られた粗抽出物中の
成分であってスチレン−ジビニルベンゼン系合成樹脂又
はデキストラン系合成樹脂に吸着される樹脂吸着成分
と、ビタミンＣ乃至ビタミンＥとを混合して得られる混
合物を有効成分とする活性酸素フリーラジカル起因疾患
予防剤。8. A component in a crude extract obtained by extracting banaba leaves with hot water, an organic solvent, or a mixed solution of an organic solvent and hot water, and is a styrene-divinylbenzene-based synthetic resin or a dextran-based resin. An active oxygen free radical-induced disease preventive agent comprising, as an active ingredient, a mixture obtained by mixing a resin adsorption component adsorbed on a synthetic resin with vitamin C to vitamin E.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9104403A JPH10291935A (en) | 1997-04-22 | 1997-04-22 | Active oxygen free radical quencher and preventive for disease caused by active oxygen free radical |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9104403A JPH10291935A (en) | 1997-04-22 | 1997-04-22 | Active oxygen free radical quencher and preventive for disease caused by active oxygen free radical |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH10291935A true JPH10291935A (en) | 1998-11-04 |
Family
ID=14379763
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9104403A Pending JPH10291935A (en) | 1997-04-22 | 1997-04-22 | Active oxygen free radical quencher and preventive for disease caused by active oxygen free radical |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH10291935A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000302634A (en) * | 1999-04-27 | 2000-10-31 | Ichimaru Pharcos Co Ltd | Cosmetic composition |
| EP1343460A4 (en) * | 2000-11-28 | 2004-09-15 | Avon Prod Inc | Anti-aging cosmetic composition and method of application |
| WO2013100718A2 (en) | 2011-12-30 | 2013-07-04 | 한국생명공학연구원 | Pharmaceutical composition for preventing or treating inflammatory diseases or asthma, containing lagerstroemia ovalifolia extract or fraction thereof as active ingredient |
| KR20200006203A (en) | 2018-07-09 | 2020-01-20 | 주식회사 레모넥스 | Pharmaceutical composition and health-functional food for preventing or treating macular degeneration |
-
1997
- 1997-04-22 JP JP9104403A patent/JPH10291935A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000302634A (en) * | 1999-04-27 | 2000-10-31 | Ichimaru Pharcos Co Ltd | Cosmetic composition |
| EP1343460A4 (en) * | 2000-11-28 | 2004-09-15 | Avon Prod Inc | Anti-aging cosmetic composition and method of application |
| WO2013100718A2 (en) | 2011-12-30 | 2013-07-04 | 한국생명공학연구원 | Pharmaceutical composition for preventing or treating inflammatory diseases or asthma, containing lagerstroemia ovalifolia extract or fraction thereof as active ingredient |
| WO2013100718A3 (en) * | 2011-12-30 | 2013-08-22 | 한국생명공학연구원 | Pharmaceutical composition for preventing or treating inflammatory diseases or asthma, containing lagerstroemia ovalifolia extract or fraction thereof as active ingredient |
| CN104185473A (en) * | 2011-12-30 | 2014-12-03 | 韩国生命工学研究院 | Pharmaceutical composition for preventing or treating inflammatory diseases or asthma, containing lagerstroemia ovalifolia extract or fraction thereof as active ingredient |
| EP2799082A4 (en) * | 2011-12-30 | 2015-07-15 | Korea Res Inst Of Bioscience | Pharmaceutical composition for preventing or treating inflammatory diseases or asthma, containing lagerstroemia ovalifolia extract or fraction thereof as active ingredient |
| US9610310B2 (en) | 2011-12-30 | 2017-04-04 | Korea Research Institute Of Bioscience And Biotechnology | Pharmaceutical composition for preventing or treating inflammatory diseases or asthma, containing lagerstroemia ovalifolia extract or fraction thereof as active ingredient |
| KR20200006203A (en) | 2018-07-09 | 2020-01-20 | 주식회사 레모넥스 | Pharmaceutical composition and health-functional food for preventing or treating macular degeneration |
| US11633447B2 (en) | 2018-07-09 | 2023-04-25 | Lemonex Inc. | Pharmaceutical composition and functional health food for preventing or treating macular degeneration |
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