JPH10276739A - Production of food and beverage containing beta-1,3-1,6-glucan or its alkali salt - Google Patents

Abstract

PROBLEM TO BE SOLVED: To eliminate a problem in which since a culture solution consisting essentially of β-1,3-1,6-glucan produced by a bacterium belonging to the genus Aureobasidium sp., etc., has a diluted concentration, when the culture solution is used as a food additive, it can hardly be determined and has difficulty in raising the concentration of component in a food. SOLUTION: A culture solution comprising β-1,3-1,6-glucan produced by a bacterium belonging to the genus Aureobasidium or β-1,3-1,6-glucan and fructooligosaccharide as a main component or a solution obtained by adding a caustic alkali to the culture solution is spray-dried or a solution obtained by ethyl alcohol to the culture solution to precipitate an aggregate, adding an aqueous solution of a caustic alkali to the aggregate and dissolving the aggregate is spray-dried to give a water-soluble powder product containing the main component or its alkali salt in high concentration.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a β-1,3-1,6-glucan or β-produced from a microorganism belonging to the genus Aureobasidium (sp).
The present invention relates to a method for producing a food and drink useful for use in health foods and the like containing 1,3-1,6 glucan and fructooligosaccharide as they are or as alkali salts at a high concentration.

[0002]

2. Description of the Related Art Aureobasidium (Aureobasidium)
cidium, sp) FERM-P. No. 4257,
ATCC. No. Β-1,3-1,
A method for producing 6 glucans, or β-1,3-1,
Methods for simultaneously producing 6 glucans and fructooligosaccharides are known from JP-A-57-149301, JP-A-61-146192 and the like. It is also known that these products have uses as foods and drinks which can be used for health maintenance drinks (proliferation of intestinal bifidus bacteria, prevention of constipation, immune enhancement), intestinal medicine, intestinal medicine for livestock, and the like. As a method for producing these, the above-mentioned bacteria of the genus Aureobasidium are treated with sucrose, rice bran, vitamin C (ascorbic acid),
Cultured in a liquid medium consisting of vitamin E etc., β-1,3-
It produces 1,6 glucans. At this time, β-1,3-1,6-glucan and fructooligosaccharide can be produced simultaneously by adjusting the sucrose concentration. The addition of ascorbic acid has the effect of promoting the production of β-1,3-1,6-glucan.

[0003]

SUMMARY OF THE INVENTION The above-mentioned Japanese Patent Application Laid-Open No. 61-14 / 1986
The technology disclosed in Japanese Patent No. 6192 is disclosed in Aureobasidium FERM-P. No. 4257, ATCC. N
o. From 20524 bacteria, fructooligosaccharides and β-1,3-1,6-glucan are simultaneously produced in a liquid medium, and this culture is sterilized by heating, and then filtered or centrifuged to be used as a food or drink. Is what you get. However, the concentration of β-1,3-1,6-glucan in the culture solution was 0.2 to 0.5% by weight, and the concentration of fructooligosaccharide was 2 to 2.
Only a low concentration of 5% by weight. This culture solution is often used by adding it to other foods and drinks as a health maintenance agent and a thickener, and because the amount of the active ingredient in the solution is low, it cannot exert sufficient efficacy and is liquid. Therefore, there is a problem that it is difficult to adjust the amount of addition.

[0004]

SUMMARY OF THE INVENTION The present invention has been made to solve the above problems, that is, β-1,3-1 produced from a microorganism belonging to the genus Aureobacidium (sp). , 6-glucan or β-1,3-1,6-glucan and a fructooligosaccharide as main components, followed by sterilization, followed by stirring and spray drying to obtain a powder. This is a method for producing food and drink containing 1,6-glucan.

Further, the present invention is characterized in that a caustic aqueous solution is added to the above-mentioned sterilized culture solution to form an alkali salt of the above-mentioned main component, and this solution is spray-dried to obtain a powder. This is a method for producing food and drink containing an alkali salt of -1,3-1,6-glucan.

In the present invention, the culture solution can be purified in advance. That is, in this method, ethyl alcohol is added to the above sterilized culture solution so as to have a concentration of 60% by volume or more, and the mixture is stirred. The precipitated aggregate is separated, and an aqueous solution of caustic alkali is added to dissolve the solution. A method for producing a food or drink containing an alkali salt of β-1,3-1,6-glucan, which comprises spray-drying a solution.

[0007] Further, the above-mentioned ethyl alcohol treatment can be performed again to re-purify. That is, in this method, water is added to the above-mentioned aggregate to dissolve it, and then ethyl alcohol is added so as to have a concentration of 60% by volume or more, and the mixture is stirred. The precipitated aggregate is separated, and an aqueous solution of caustic alkali is added to dissolve the aggregate. A method for producing an alkali salt of β-1,3-1,6-glucan, wherein the solution is spray-dried to obtain a powder.

[0008]

DETAILED DESCRIPTION OF THE INVENTION Aureobasidium (Aurea)
obacidium, sp) FERM-P. No. 42
57, ATCC. Β- produced from No. 20254 bacteria
A culture solution obtained by sterilizing a culture solution containing 1,3-1,6-glucan as a main component is commercially available from Sophie Co., Ltd. This β-1,3-1,6-glucan has three β-1,6 linked glucoses per four β-1,3 linked glucoses in the main chain, and a 2.5% sulfoacetic acid linked glucose. Sugars. Commercially available culture medium has viscosity and β-
The concentration of 1.3-1,6 glucan is about 0.25 to 0.4% by weight.

The present invention will be described below with reference to the drawings. When this culture solution is spray-dried, if the spraying is performed as it is, the spray nozzle is likely to be clogged because the viscosity is large and fine fibrous substances are present. Therefore, the culture solution is stirred with a high-speed mixer to pulverize the fibrous substance and spray-dry. Add a caustic aqueous solution to the culture and add pH.
= 9 to 10 to produce an alkaline salt of β-1,3-1,6-glucan. As the caustic alkali, caustic soda and caustic potash are preferable. This β-1,3-1,
Spray drying of a solution containing a 6-glucan alkali salt as a main component is performed. The solution of the alkali salt need not be subjected to high-speed mixing. The temperature for performing spray drying is about 120 to 150
° C, and thus a powder product of β-1,3-1,6-glucan or an alkali salt thereof can be obtained (target substance). Alkali salts, especially sodium and potassium salts, are very soluble in water and are advantageous when used in thickeners or food additives.

According to another aspect of the present invention, when ethyl alcohol is added to the above-mentioned sterilized culture solution so that the concentration in the whole solution becomes 60% by volume or more, the solution of ethyl alcohol and the culture solution is added. A thin film is formed on the surface, and an agglomerated substance instantaneously precipitates upon stirring. The agglomerates are large and very easy to filter. In this case, the concentration of ethyl alcohol is important, and is preferably 60 to 70% by volume. If it is less than 60% by volume, β-1,3-1,6-glucan is not sufficiently precipitated,
Remains in the liquid. Further, even if it exceeds 70% by volume, ethyl alcohol is consumed more than necessary with little change in the amount precipitated. The precipitated aggregates are collected by filtration or centrifugation, and when a diluted caustic alkali aqueous solution of about 0.001N is added and sufficiently dissolved, the alkali salt of β-1,3-1,6-glucan (preferably sodium Salt or potassium salt), and this solution is spray-dried in the same manner as described above to obtain a powder product (target substance). Note that ascorbic acid contained in the culture solution migrates into the filtrate and is colored yellow.
When the product is white and made into an aqueous solution, it is viscous and has high commercial value as food and drink.

The aggregate obtained by filtration as described above can be dissolved in water and re-purified. In other words, ethyl alcohol is added to this aqueous solution at a concentration of 60% by volume.
The same powder product is obtained by adding and stirring as described above, separating the precipitated aggregate by filtration or centrifugation, dissolving by adding a dilute caustic aqueous solution of about 0.001N, and spray drying. Obtained (target substance). As described above, the culture solution containing β-1,3-1,6-glucan as the main component has been described. However, the culture solution containing β-1,3-1,6-glucan and fructooligosaccharide as the main components may be used. Similarly, a powder product can be obtained. It should be noted that the yield of the main component in the product can be substantially the theoretical amount from the main component in the culture solution.

Hereinafter, the present invention will be described specifically with reference to examples. Example 1 β produced from a microorganism belonging to the genus Aureobasidium
One liter (about 1 kg) of a sterilized culture solution containing 0.25 to 0.3% by weight of -1,3-1,6 glucan was collected,
High-speed mixing was performed by a mixer for 10 minutes, and spray drying was performed. The spray dryer has a diameter of 700 mm and a height of 20
It is a SUS316 container of 00 mm. The temperature of the spray drying was 120 to 130 ° C., and the above culture solution was dropped over 1 hour to produce 3 g of β-1,3-1,6-glucan powder. This product is colored reddish-brown, but its purity is about 1
It is 00% and can be used as an additive for food and drink.

Example 2 1 liter of the same culture solution as in Example 1 was collected, and
A 001N aqueous solution of sodium hydroxide was added to adjust the pH to 10. Using the same spray dryer as in Example 1, this solution was dropped under the same conditions over 1 hour to produce about 3 g of β-1,3-1,6-glucan sodium salt powder. The product has the same appearance as in Example 1, but is very water-soluble and useful as an additive in foods and drinks.

Example 3 β produced from a microorganism belonging to the genus Aureobasidium
200 ml of a sterilized culture solution containing 0.4% by weight of -1,3-1,6-glucan was collected, and ethyl alcohol 30
When 0 ml was added and stirred, fibrous aggregates precipitated. This was subjected to suction filtration to obtain 2.5 g of a water-containing residue and 500 ml of an alcohol-water mixed filtrate. The filtrate contains ascorbic acid and is colored yellow. To the obtained residue, 200 ml of an aqueous solution of caustic soda (pH = 9.5) was added and dissolved at 70 ° C. for 1 hour. The solution was dried by the same spray dryer as in the previous example, and as a result, 0.6 g of white powder of sodium salt of β-1,3-1,6-glucan was obtained. Since the sample is small, it is difficult to collect the whole amount from the container. The product is very water-soluble and has no color and is useful as an additive in food and drink.

[0015]

According to the present invention, β having an immunopotentiating action
It is possible to obtain a powder product containing -1,3-1,6 glucan or fructooligosaccharide which is a human bifidus factor, or an alkali salt thereof at a high concentration.
It is extremely useful for application to health-maintaining foods or beverages, as well as for use as a thickener to give foods richness and enhance tactile sensation. That is, the powder product according to the present invention contains almost 100% of these components, so that the quantification at the time of addition is extremely easy, and the added food is compared with the case where the diluted culture solution having a sterilized main component concentration is added as it is. The active ingredient concentration therein can be increased. Furthermore, when β-1,3-1,6-glucan or the like in the powder product according to the present invention is an alkali salt, it is very soluble in water, and is very convenient when added to beverages and the like.

[Brief description of the drawings]

FIG. 1 is a process chart of the present invention.

Claims (4)

[Claims] (1) Aureobasidium (Aureobac)
β-produced by microorganisms belonging to Idium, sp)
After sterilizing a culture solution containing 1,3-1,6-glucan or β-1,3-1,6-glucan and fructooligosaccharide as main components, stirring, spray-drying, and obtaining a powder. A method for producing food and drink containing β-1,3-1,6-glucan. 2. Aureobasidium (Aureobac)
β-produced by microorganisms belonging to Idium, sp)
A culture solution containing 1,3-1,6-glucan or β-1,3-1,6-glucan and fructooligosaccharide as main components is sterilized, and an aqueous solution of caustic alkali is added to form an alkali salt of the main component. A method for producing a food or drink comprising an alkali salt of β-1,3-1,6-glucan, wherein the solution is spray-dried to obtain a powder. 3. A culture solution subjected to the sterilization treatment according to claim 2, wherein ethyl alcohol is added so as to have a concentration of 60% by volume or more, and the mixture is stirred to separate precipitated aggregates. And then subjecting the solution to spray drying to produce a food or drink containing an alkali salt of β-1,3-1,6-glucan. 4. The aggregate according to claim 3, which is dissolved by adding water thereto, adding ethyl alcohol so as to have a concentration of 60% by volume or more and stirring to separate the aggregate which precipitates. A method for producing an alkali salt of β-1,3-1,6-glucan, characterized in that a powder is obtained by spray-drying the solution after adding and dissolving the solution.