JPH10267887A - Sensor device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To enable the reduction of time and labor in measurements and the dispersion in measurement data by arranging a plurality of electrodes with a working electrode and a counter electrode at regular intervals and integrating all electrode parts and the output terminals of measurement data. SOLUTION: The first protrusions 2 are provided at predetermined intervals for the one side of a square insulating substrate 1, and the second protrusion 3 of a predetermined width is provided for the center of another side. A working electrode 2a, a counter electrode 2b, and a reference pole 2c are formed on the surface of the first protrusion 2, and derived terminals 3a are formed at predetermined intervals on the surface of the second protrusion 3. These electrode parts and the derived terminals 3a are electrically connected by wirings 1a. The first protrusions 2 are each penetrated into corresponding cells, and a sample is brought into contact with the electrode parts. The derived terminals 3a of the second protrusion 3 are connected to a signal processing device via a connector and an amplifier 7. As it is possible to insert each of a plurality of electrode parts into a cell in one operation by this structure, it is possible to reduce labor in measurements. In addition, as the state of connection becomes uniform, it is possible to reduce the dispersion in the values of measurements.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sensor device, and more particularly, to measuring a sample (eg, a solution to be measured) using a microplate having a plurality of rows and a plurality of columns of sample storage chambers. It relates to a suitable sensor device.

[0002]

2. Description of the Related Art Conventionally, as an instrument for accommodating a specimen, a microplate (a microplate for a high-throughput system) having a plurality of rows and a plurality of columns of specimen accommodating chambers (cells) has been provided. The microplate has a standardized size, the number of cells, the cell pitch, and the like, and generally has eight rows and twelve columns of cells.

[0003] For example, in measuring the amount of dissolved oxygen, a sensor configured as a single unit has conventionally been used. That is, a sensor configured as a single body is made to enter an appropriate cell to come in contact with a sample contained therein, and an electric signal indicating a measurement result is taken out.

[0004]

In the case of performing the above-described measurement, a sensor configured as a single unit is sequentially inserted into each cell, or a plurality of sensors configured as a single unit are respectively inserted into corresponding cells. Since the sample must be penetrated and brought into contact with the sample, there is a disadvantage that not only the measurement is troublesome but also the measurement data is caused to vary. In addition, there is an inconvenience that the number of samples that can be measured at one time is limited due to spatial restrictions and restrictions on handling.

[0005]

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned problems, and can greatly reduce the time and effort required for measurement, greatly reduce the dispersion of measurement data, and reduce the number of samples that can be measured at one time. It is an object of the present invention to provide a sensor device capable of relaxing restrictions.

[0006]

According to a first aspect of the present invention, a plurality of electrode units having at least a working electrode and a counter electrode are arranged at predetermined intervals, and all the electrode units are integrated. In addition, a plurality of lead terminals for outputting a measurement signal of each electrode unit are also integrated.

According to a second aspect of the present invention, there is provided a sensor device having projecting portions at predetermined positions on the outer periphery of a flat plate-shaped base at predetermined intervals, and having at least a working electrode and a counter electrode at predetermined positions of the projecting portion. Among them, a plurality of lead terminals for outputting a measurement signal of each electrode portion are provided at a predetermined position facing a portion where the protrusion is formed. According to a third aspect of the present invention, the sensor device further includes a connector to which the extraction terminal is detachably connected, and an amplifier for amplifying the measurement signal extracted through the extraction terminal, and the connector and the amplifier are integrated.

According to a fourth aspect of the present invention, there is provided a sensor device in which a plurality of bases are connected in parallel with each other so as to be detachable as connectors, and the amplifier amplifies measurement signals taken out through the lead-out terminals of the plurality of bases. This is what was adopted. The sensor device according to claim 5 has a plurality of sample storage chambers, and an electrode section having at least a working electrode and a counter electrode is formed at the bottom of each sample storage chamber, and outputs a measurement signal of each electrode section. Are also integrated.

A sensor device according to a sixth aspect of the present invention has a plurality of rows and a plurality of columns of projections projecting at a predetermined angle from one surface of a flat substrate, and has at least a working electrode at a predetermined position of the projection. And a plurality of extraction terminals for outputting a measurement signal of each electrode portion at a predetermined position on an edge of the base. The sensor device according to claim 7 has a plurality of sample storage chambers, and an electrode section having at least a working electrode and a counter electrode is formed on the bottom inner surface of each sample storage chamber, and a measurement signal of each electrode section is transmitted. A plurality of output terminals for output are provided so as to be exposed on the outer surface of the bottom of each sample storage chamber.

[0010] The sensor device according to claim 8 has a plurality of sample accommodating chambers, and has an inward projection extending inward from the center of the inner peripheral surface of each sample accommodation chamber. An electrode portion is formed at a predetermined position on the outer surface, the electrode portion has at least a working electrode and a counter electrode, and a plurality of extraction terminals for outputting a measurement signal of each electrode portion are provided on the outer surface at the bottom of each sample storage chamber. It is provided in a state where it is exposed.

According to a ninth aspect of the present invention, a shaft portion extending upward from a central portion of the bottom inner surface of each sample accommodating chamber is employed as an inwardly projecting portion, and a working electrode and a reference electrode are provided on an outer surface of the shaft portion as an electrode portion. And having a counter electrode on the upper surface of the bottom.

[0012]

According to the sensor device of the first aspect, a plurality of electrode portions having at least a working electrode and a counter electrode are arranged at predetermined intervals, and all the electrode portions are integrated,
In addition, since a plurality of lead terminals for outputting the measurement signal of each electrode unit are also integrated, the multiple electrode units and the lead terminals can be handled as a single unit, greatly reducing the labor and time required for measurement. Variations in data can also be significantly reduced. Since a plurality of electrode portions are arranged at predetermined intervals, spatial restrictions and restrictions on handling can be greatly reduced, and the restrictions on the number of samples that can be measured at one time can be reduced.

According to a second aspect of the present invention, there is provided a sensor device having projections at predetermined positions on the outer periphery of a flat substrate at predetermined intervals, and having at least a working electrode and a counter electrode at predetermined positions of the projections. Since the base has a plurality of lead terminals for outputting a measurement signal of each electrode at a predetermined position facing the portion where the protrusion is formed, the same operation as in claim 1 is employed. Can be achieved.

According to a third aspect of the present invention, the sensor device further includes a connector to which the extraction terminal is detachably connected, and an amplifier for amplifying the measurement signal extracted through the extraction terminal, wherein the connector and the amplifier are integrated. Is adopted, the measurement signal having a low level can be immediately amplified, the noise resistance can be improved, and the same operation as in claim 2 can be achieved.

According to a fourth aspect of the present invention, as the connector, a connector for connecting a plurality of bases in parallel with each other so as to be detachable is employed as a connector, and as an amplifier, a measurement signal taken out through a lead terminal of the plurality of bases is used. Since amplifying each is adopted, the restriction on the number of samples that can be measured at one time can be further relaxed.
The same operation as described above can be achieved.

According to a fifth aspect of the present invention, there is provided a sensor device having a plurality of sample accommodating chambers, and an electrode section having at least a working electrode and a counter electrode is formed at the bottom of each sample accommodating chamber. Since a plurality of lead terminals for outputting the measurement signal are integrated, the plurality of electrode portions and the lead terminals can be handled as one unit,
The same operation as described above can be achieved.

According to the sensor device of the sixth aspect, a plurality of rows projecting at a predetermined angle from one surface of the flat substrate,
It has a plurality of rows of protrusions, has at least a working electrode and a counter electrode at predetermined positions of the protrusions, and has a plurality of lead terminals for outputting measurement signals of each electrode part at predetermined positions of the edge of the base. As a result, the time and effort for measurement can be further greatly reduced, and the same effect as in claim 1 can be achieved.

According to the sensor device of the present invention, an electrode portion having at least a working electrode and a counter electrode is formed on a bottom inner surface of each of the sample storage chambers. Since the plurality of extraction terminals that output the measurement signals of the sections are provided so as to be exposed on the bottom outer surface of each sample storage chamber, the plurality of electrode sections and the extraction terminals can be handled integrally, and The labor and time can be greatly reduced, and the variation of the measurement data can be significantly reduced. Since a plurality of electrode portions are arranged at predetermined intervals, spatial restrictions and restrictions on handling can be greatly reduced, and the restrictions on the number of samples that can be measured at one time can be reduced.

In the sensor device according to the present invention, there are provided a plurality of sample accommodating chambers, and an inward projection extending inward from a central portion of the inner peripheral surface of each sample accommodating chamber. An electrode portion is formed at a predetermined position on the outer surface of the portion, the electrode portion has at least a working electrode and a counter electrode, and a plurality of extraction terminals for outputting a measurement signal of each electrode portion are provided at the bottom of each sample storage chamber. Since it is provided so as to be exposed on the outer surface, it is possible to handle a plurality of electrode parts and lead terminals as a single unit, so that it is possible to greatly reduce the labor of measurement and also greatly reduce the dispersion of measurement data. . Since a plurality of electrode portions are arranged at predetermined intervals, spatial restrictions and restrictions on handling can be greatly reduced, and the restrictions on the number of samples that can be measured at one time can be reduced. Further, the area between the working electrode and the counter electrode can be easily increased without changing the inner diameter of the sample storage chamber.

In the sensor device according to the ninth aspect, the shaft portion extending upward from the center of the bottom inner surface of each sample accommodating chamber is employed as the inward projection, and the working electrode is referred to as the electrode portion on the outer surface of the shaft portion. Since the one having the pole and the opposite pole on the upper surface of the bottom portion is employed, the same operation as the eighth aspect can be achieved.

[0021]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments of the sensor device according to the present invention will be described in detail with reference to the accompanying drawings. FIG. 1 is a front view showing an embodiment of the sensor device of the present invention. In this sensor device, a first protruding portion 2 is formed at predetermined intervals on one long side of an insulating substrate 1 having a rectangular shape, and a second protruding portion 3 having a predetermined width is formed at the center of the other long side. Is formed. Then, a working electrode 2a, a counter electrode 2b, and a reference electrode 2c constituting an electrode portion are formed on the surface of each first protrusion 2.
Is formed. Further, lead terminals 3a are formed at predetermined intervals on the surface of the second protruding portion 3, and the working electrode 2a and the counter electrode 2a are formed.
The wiring 1a for electrically connecting the reference electrode b and the reference electrode 2c to the extraction terminal 3a is formed. The working electrode 2a, the counter electrode 2b, the reference electrode 2c, the lead terminal 3a, and the wiring 1
a is preferably formed by using a printing technique. In addition, the sensor device configured as described above may detect the amount of dissolved oxygen in the sample, or may detect hydrogen peroxide or the like.

FIG. 2 is a schematic diagram showing a state in which a sample is measured using the sensor device having the configuration shown in FIG. In FIG. 2, reference numeral 4 denotes a microplate, in which cells 4a formed at predetermined intervals store samples 4b, respectively. Then, the first protruding portions 2 of the sensor device having the configuration of FIG. 1 are made to penetrate into the corresponding cells 4a, respectively, so that the electrode portions and the sample 4b are brought into contact. The extraction terminal 3a of the second protrusion 3 of the sensor device is connected to the connector 5, and the connector 5 is connected to the signal processing device 8 such as a computer via the electric wire 6 and the amplifier 7. As the signal processing, a conventionally known endpoint method, rate method, or the like can be employed.

As is apparent from FIG. 2, a plurality of electrode portions can be made to enter the corresponding cell 4a by one operation, and the connection with the connector 5 can be achieved by one operation. As a whole, the labor for measurement can be greatly reduced. In addition, the first projecting portion 2 is
Since the intervals are set so as to correspond to a, spatial restrictions and restrictions on handling can be greatly reduced, and the restrictions on the number of samples that can be measured at one time can be relaxed. Furthermore, since the contact state between all the electrode units and the specimen, the temperature environment, the connection state with the connector 5, and the like can be made substantially uniform, the variation in measurement data can be greatly reduced.

FIG. 3 is a schematic diagram showing a state in which a sample is measured using the sensor device having the configuration shown in FIG. In FIG. 3, a connector 5 is used which has a configuration in which a plurality of sensor devices having the configuration of FIG. 1 can be connected and held in parallel with each other, and an amplifier 7 is provided integrally with the connector 5. However, only the configuration is different from that of FIG. 2, and the configuration of the other parts is the same.

In the case of FIG. 3, the number of samples which can be measured at one time can be greatly increased as compared with the case of FIG. 2, and the measurement signal taken out through the lead-out terminal 3a and the connector 5 is immediately amplified by the amplifier. 7, the influence of noise on the measurement signal before amplification is greatly reduced and the measurement accuracy can be significantly increased as compared with the case of FIG. In addition, the same operation as in the case of FIG. 2 can be achieved.

FIG. 17 is a block diagram showing an electrical configuration corresponding to this embodiment. That is, the measurement signals output from the plurality of sensor devices S are amplified by the corresponding preamplifiers P, and are selectively supplied to the signal processing device 8 via the multiplexer M. Note that the signal transmission between the multiplexer M and the signal processing device 8 may be performed via a wire, or may be performed by communication using RS232C. Further, the signal processing device 8 may perform a smoothing process or the like as a measure against noise.
A microcomputer may be mounted on each preamplifier P to perform the same processing.

In adopting the configuration of FIG. 3, as shown in FIG. 4, a guide rail 10 is provided on the periphery of a table 9 on which the microplate 4 is positioned and placed, and the guide rail 10 is provided on the periphery of the connector 5. If the configuration in which the engaging member 11 is engaged is adopted, the first protruding portion 2 can be surely made to enter the corresponding cell 4a simply by pushing down the connector 5, and the operability can be improved.

FIG. 5 is a view showing another embodiment of the sensor device of the present invention. The right half shows a front view and the left half shows a rear view. In the sensor device of FIG.
A working electrode 2a and a reference electrode 2c on the front side of the first protrusion 2;
Since each of the opposing electrodes 2b is provided on the back side of the first protruding portion 2, the area of the working electrode 2a having the largest influence on the measurement signal intensity can be increased as compared with the sensor device of FIG. As a result, the measurement sensitivity can be improved. In addition, the same operation as that of the sensor device of FIG. 1 can be achieved.

FIG. 6 is a schematic perspective view showing a main part of still another embodiment of the sensor device of the present invention. FIG. 6 shows the bottom surface of one cell of the microplate. The working electrode 2a is formed at the center of the bottom surface, and the counter electrode 2b and the counter electrode 2b are formed so as to surround almost the entire range of the working electrode 2a. The reference electrode 2c is provided in each of the portions not shown. Although not shown, the lead terminals and the wirings may be formed in a microplate, for example, or may be formed on the surface of the microplate.

When the embodiment shown in FIG. 6 is adopted, the microplate is connected to the signal processing unit through a connector, an amplifier and wiring, and in this state, a microplate pipette or the like is used for each cell 4a. The measurement can be performed only by supplying the sample with the sample, and the labor of the measurement can be greatly reduced. In addition, since there is no need for the electrode section to penetrate the cell, the dispersion of measurement data can be greatly reduced, and the number of samples that can be measured at one time can be measured at one time with almost no spatial or handling restrictions. Cell number.

FIG. 7 is a plan view showing still another embodiment of the sensor device of the present invention, and FIG. 8 is a longitudinal sectional view at the center. This sensor device includes a partition wall member 2 on an upper surface of an insulating substrate 21.
2 are fixed by bonding or the like to form a plurality of cells 23, and a working electrode 24 and a counter electrode 25 are formed on the bottom surface of each cell 23.
Is provided. Then, a lead terminal 26 is formed at one edge of the insulating substrate 21, and a wiring 27 for electrically connecting the working electrode 24, the counter electrode 25 and the lead terminal 26 is formed.

Therefore, even when this embodiment is adopted, the same operation as that of the embodiment of FIG. 6 can be achieved. FIG. 9 is a plan view showing still another embodiment of the sensor device of the present invention, and FIG. 10 is a central longitudinal sectional view. In this sensor device, a cutout portion is provided at a predetermined position of an insulating substrate 31 having a plane shape substantially equal to that of the microplate 4 so as to correspond to each cell 4a of the microplate 4, and the cutout portion is formed by the cutout portion. The part 32 where the work is allowed
, A working electrode 33 and a counter electrode 34 are formed. A lead terminal 35 is formed at one edge of the insulating substrate 31, and a wiring 36 for electrically connecting the working electrode 33 and the counter electrode 34 to the lead terminal 35 is formed.

Therefore, when this embodiment is adopted, each electrode portion can be made substantially perpendicular to the insulating substrate 31 as shown in FIG. By moving the insulating substrate 31 in the above, each of the electrode portions is made to correspond to the corresponding cell 4.
a. As a result, the labor of measurement can be greatly reduced. In addition, since all the electrodes enter the cell almost at the same time, the dispersion of measurement data can be greatly reduced, and the number of samples that can be measured at one time with almost no spatial or handling restrictions is greatly reduced. Can be relaxed.

In this embodiment, the insulating substrate 3
It is also possible to adopt a corrugated plate shape as 1 and to adopt a shape that substantially conforms to the inner surface of the cell 4a when pulled out and raised as the portion 32 where pulling out is allowed.
By adopting this modification, it is possible to easily supply a specimen using a microplate pipette or the like in a state where the electrode portion is made to intrude into all the cells 4a of the microplate 4, and the above embodiment is different from the above embodiment. A similar effect can be achieved.

FIG. 11 is a perspective view showing still another embodiment of the sensor device of the present invention. In this sensor device, first protrusions 42 are provided at predetermined intervals on both long sides of a long base 41. The first protrusion 42 has a working electrode (not shown), a counter electrode, and a reference electrode, if necessary. A lead terminal (not shown) is formed at an end of the base 41, and a wiring connecting the working electrode, the counter electrode, and the reference electrode to the lead terminal is formed.

Even when this embodiment is adopted, the labor required for measurement can be greatly reduced as a whole, and the spatial and handling restrictions can be significantly reduced. Number constraints can be relaxed. Furthermore, since the contact state, the temperature environment, the connection state with the connector, and the like of all the electrode units and the specimen can be made substantially uniform, the variation in the measurement data can be greatly reduced.

FIG. 12 is an electric circuit diagram showing an example of the electric configuration of the amplifier 7. This amplifier 7 corresponds to an electrode section consisting of only a working electrode and a counter electrode. FIG.
2, the working electrode is indicated by W, and the counter electrode is indicated by C. The amplifier 7 supplies an output signal from a working electrode to a non-inverting input terminal of an operational amplifier 51, connects an inverting input terminal to the ground GND, and connects a resistor 52 between the non-inverting input terminal and the output terminal. The output signal from the output terminal is output as an amplified signal. Also, a bias voltage is applied to the non-inverting input terminal of the operational amplifier 53, the inverting input terminal is connected to the ground GND, the resistor 54 is connected between the non-inverting input terminal and the output terminal, and the output voltage from the output terminal is Is applied to the opposite pole.

Therefore, a bias voltage is applied to the counter electrode via the operational amplifier 53, and in this state, the measurement signal output from the working electrode can be amplified and output by the operational amplifier 51. FIG. 13 is an electric circuit diagram showing another example of the electric configuration of the amplifier 7. The amplifier 7 corresponds to an electrode section including a working electrode, a counter electrode, and a reference electrode. In FIG. 13, the working electrode is indicated by W, the counter electrode is indicated by C, and the reference electrode is indicated by R.

The amplifier 7 applies a bias voltage to the non-inverting input terminal of the operational amplifier 55 via the resistor 56, connects the inverting input terminal to the ground GND, and applies the output voltage from the output terminal to the opposite pole. ing. Further, the output signal from the reference electrode is supplied to the inverting input terminal of the operational amplifier 57, the non-inverting input terminal and the output terminal are short-circuited, and the output voltage from the output terminal is supplied to the non-inverting terminal of the operational amplifier 55 through the resistor 58. Applied to the input terminal. Then, diodes 59 and 60 having opposite polarities are connected in parallel between the non-inverting input terminal and the inverting input terminal of the operational amplifier 57. further,
An output signal from the working electrode is supplied to an inverting input terminal of the operational amplifier 61, and a resistor 62 is connected between the non-inverting input terminal and the output terminal.
And outputs the output signal from the output terminal as an amplified signal. Then, in order to cope with a case where noise or the like is superimposed on the output signal supplied to the non-inverting input terminal of the operational amplifier 61, a predetermined positive voltage and a predetermined negative voltage are respectively supplied through the reverse-connected diodes 63 and 64. Is being applied.

Therefore, a bias voltage based on the reference electrode is applied to the opposite electrode via the operational amplifiers 55 and 57,
In this state, the measurement signal output from the working electrode can be amplified by the operational amplifier 61 and output. FIG.
4 is an electric circuit diagram showing still another example of the electric configuration of the amplifier 7. The amplifier 7 corresponds to an electrode section including a working electrode, a counter electrode, and a reference electrode. FIG.
In 4, the working electrode is indicated by W, the counter electrode is indicated by C, and the reference electrode is indicated by R.

The amplifier 7 supplies the output signal from the reference pole to the non-inverting input terminal of the operational amplifier 65 via the resistor 66, applies a bias voltage to the inverting input terminal, and connects the non-inverting input terminal to the output terminal. , A capacitor 67 is connected therebetween, and an output voltage from an output terminal is applied to a counter electrode via a resistor 68. The output signal from the working electrode is converted to an operational amplifier 6.
9, a resistor 70 and a capacitor 71 are connected in parallel between the non-inverting input terminal and the output terminal, and an output signal from the output terminal is output as an amplified signal. The inverting input terminal of the operational amplifier 69 is connected to an appropriate bias.

Therefore, a bias voltage based on the reference electrode is applied to the counter electrode via the operational amplifier 65, and in this state, the measurement signal output from the working electrode can be amplified and output by the operational amplifier 69. . FIG. 15 is an electric circuit diagram showing still another example of the electric configuration of the amplifier 7. The amplifier 7 corresponds to an electrode section including a working electrode, a counter electrode, and a reference electrode. In FIG. 15, the working electrode is indicated by W, the counter electrode is indicated by C, and the reference electrode is indicated by R.
Indicated by.

The amplifier 7 applies a bias voltage to the non-inverting input terminal of the operational amplifier 76 via a resistor 77, connects the inverting input terminal to the ground GND, and outputs the output voltage from the output terminal via the resistor 72. Applied to the opposite pole. Further, the output signal from the reference electrode is supplied to the inverting input terminal of the operational amplifier 73, the non-inverting input terminal and the output terminal are short-circuited,
The output voltage from the output terminal is applied to the non-inverting input terminal of the operational amplifier 76 via the resistor 74. Further, the output signal from the working electrode is supplied to the ground GND, and the voltage across the resistor 72 is applied to the inverting input terminal of the operational amplifier 75, respectively.
An output signal applied to the non-inverting input terminal and output from the output terminal is used as an amplified signal.

Therefore, a bias voltage based on the reference electrode is applied to the opposite electrode via the operational amplifiers 73 and 76,
In this state, the measurement signal output from the working electrode can be amplified and output by the operational amplifiers 75 and 76. FIG. 16 shows a sample consisting of only a culture medium using a part of the electrode part of the sensor device having the configuration of FIG.
10 shows the results of measuring the transition of the amount of dissolved oxygen in each of the specimens to which 10% of E. coli) was added.
Note that the horizontal axis is time (minutes) and the vertical axis is the detected current (n
A). Also, a indicates the former case, and b indicates the latter case.

As is apparent from FIG. 16, when E. coli was added, it was found that the amount of dissolved oxygen was reduced by the respiration of E. coli, and the amount of dissolved oxygen was measured using the sensor device of the present invention. We can see that we can do it.
In addition, the dissolved oxygen amount is also gradually decreased in the sample composed of only the culture medium, which seems to indicate a decrease in the dissolved oxygen amount due to the influence of the electrode portion.

FIG. 18 is an exploded perspective view showing still another embodiment of the sensor device of the present invention, FIG. 19 is an enlarged longitudinal sectional view of the essential part, and FIG. 20 is a plan view of FIG. In this sensor device, a large number of sample storage chambers 4a are configured by disposing a top plate 85 having a large number of columnar through holes integrally on a bottom plate 86 and integrating them in a watertight manner. The working electrode 2a is formed at the center of the upper surface of the bottom plate at a position corresponding to the bottom wall of the sample storage chamber 4a, and the counter electrode 2b and the reference electrode 2b are formed.
and c are formed in a ring shape centering on the working electrode 2a. Note that the size of the counter electrode 2b and the reference electrode 2c is set so that the former is larger than the latter. The working electrode 2a, the counter electrode 2b, and the reference electrode 2c form an electrode portion.

The working electrode 2a, the counter electrode 2b, and the working electrode 2a are provided on the lower surface of the bottom plate at a position corresponding to the bottom wall of the sample accommodating chamber 4a.
Lead terminals 3a, 3b, 3c corresponding to the reference electrode 2c
And the working electrode 2a, the lead terminal 3a, and the counter electrode 2b
And a lead terminal 3b, and a connection portion (for example, a through portion made of silver paste) for electrically connecting the reference electrode 2c and the lead terminal 3c. FIG. 19 shows contacts 81, 82, and 83 that are electrically connected to the lead terminals 3a, 3b, and 3c, respectively.

When the sensor device having this configuration is employed, the sample is dispensed into the sample accommodating chamber 4a, and the drawer terminals 3a, 3b, 3c are brought into electrical contact with the contacts 81, 82, 83, respectively. Alone, it is possible to simultaneously measure many analytes. Also, the sample storage room 4a
Since the electrode portion is provided integrally with the electrode, spatial restrictions and restrictions on handling can be greatly reduced, and the restrictions on the number of samples that can be measured at one time can be reduced. Further, the contact state between all the electrode units and the specimen, the temperature environment, the connection state with the contactor, and the like can be made substantially uniform, so that the variation in measurement data can be significantly reduced.

FIG. 21 is a partially cutaway perspective view showing a part of still another embodiment of the sensor device of the present invention, and FIG. 22 is a longitudinal sectional view of the same. This sensor device is different from the above-described sensor device in that the bottom plate has a shaft portion 84 extending upward from a central portion of the upper surface of a portion corresponding to the bottom wall portion of the sample storage chamber 4a. 84, a reference electrode 2c is formed on the upper surface;
The working electrode 2a is formed on the side surface of the shaft portion 84, and the shaft portion 84a is formed on the upper surface of a portion of the bottom plate corresponding to the bottom wall portion of the sample storage chamber 4a.
The only difference is that a concentric counter electrode 2b is formed.

Therefore, when this embodiment is adopted, the areas of the working electrode 2a and the counter electrode 2b can be increased without increasing the inner diameter of the sample accommodating chamber 4a. A similar effect can be achieved.

[0051]

According to the first aspect of the present invention, a plurality of electrode portions and lead-out terminals can be handled integrally, so that the trouble of measurement can be greatly reduced, and the dispersion of measurement data can be greatly reduced. Since a plurality of electrodes are arranged at predetermined intervals, spatial restrictions and restrictions on handling can be significantly reduced, and the unique effect that the restriction on the number of samples that can be measured at once can be relaxed. Play.

The second aspect of the invention has the same effect as the first aspect. According to the third aspect of the present invention, a measurement signal having a low level can be immediately amplified, the noise resistance can be improved, and the same effect as that of the second aspect can be obtained. Claim 4
According to the invention, the restriction on the number of samples that can be measured at one time can be further alleviated, and the same effect as that of claim 3 can be obtained.

The fifth aspect of the invention has the same effect as the first aspect. According to the sixth aspect of the present invention, the trouble of the measurement can be further reduced, and the same effect as that of the first aspect can be obtained. According to the seventh aspect of the present invention, the plurality of electrode portions and the lead-out terminal can be handled integrally, so that the trouble of the measurement can be greatly reduced, and the variation of the measurement data can be greatly reduced. Are arranged at predetermined intervals, so that the spatial and handling restrictions can be greatly reduced, and the unique effect that the restrictions on the number of samples that can be measured at one time can be relaxed.

According to the eighth aspect of the present invention, the plurality of electrode portions and the lead terminals can be handled integrally, so that the trouble of the measurement can be greatly reduced, and the dispersion of the measurement data can be greatly reduced. Since the electrodes are arranged at predetermined intervals, spatial restrictions and restrictions on handling can be greatly reduced, and thus the restrictions on the number of samples that can be measured at one time can be eased. This has a unique effect that the area between the working electrode and the counter electrode can be easily increased without changing the inner diameter.

The ninth invention has the same effect as the eighth invention.

[Brief description of the drawings]

FIG. 1 is a front view showing one embodiment of a sensor device of the present invention.

FIG. 2 is a schematic diagram showing a state where a sample is measured using the sensor device having the configuration of FIG. 1;

FIG. 3 is a schematic diagram showing a state where a sample is measured using the sensor device having the configuration of FIG. 1;

FIG. 4 is a schematic vertical sectional view showing a modification of FIG. 3;

FIG. 5 is a diagram showing another embodiment of the sensor device of the present invention.

FIG. 6 is a schematic perspective view showing a main part of still another embodiment of the sensor device of the present invention.

FIG. 7 is a plan view showing still another embodiment of the sensor device of the present invention.

FIG. 8 is a central longitudinal sectional view of the same.

FIG. 9 is a plan view showing still another embodiment of the sensor device of the present invention.

FIG. 10 is a central longitudinal sectional view of the same.

FIG. 11 is a perspective view showing still another embodiment of the sensor device of the present invention.

FIG. 12 is an electric circuit diagram showing an example of an electric configuration of the amplifier.

FIG. 13 is an electric circuit diagram showing another example of the electric configuration of the amplifier.

FIG. 14 is an electric circuit diagram showing still another example of the electric configuration of the amplifier.

FIG. 15 is an electric circuit diagram showing still another example of the electric configuration of the amplifier.

FIG. 16 shows a sample consisting of only a medium and a medium containing Escherichia coli (E.
FIG. 10 is a diagram showing the results of measuring changes in the amount of dissolved oxygen in each of the samples obtained by adding 10% of E. coli).

FIG. 17 is a block diagram showing an electrical configuration corresponding to FIG. 3;

FIG. 18 is an exploded perspective view showing still another embodiment of the sensor device of the present invention.

FIG. 19 is an enlarged longitudinal sectional view of the main part.

FIG. 20 is a plan view of FIG. 19;

FIG. 21 is a partial cutaway perspective view showing a part of still another embodiment of the sensor device of the present invention.

FIG. 22 is a longitudinal sectional view of the same.

[Explanation of symbols]

1, 31 Substrate 1a, 27, 35 Wiring 2, 32 Projection 2a, 24, 33 Working electrode 2b, 25, 34 Counter electrode 3a, 26, 35 Lead-out terminal 4 Microplate 4a Cell 5 Connector 7 Amplifier 84 Shaft

Claims (9)

[Claims] At least the working electrodes (2a), (24) and (3)
3) and a plurality of electrode portions each having a counter electrode (2b) (25) (34) are arranged at predetermined intervals, all the electrode portions are integrated, and the measurement signal of each electrode portion is also measured. (3a) (26) (3
5) A sensor device, wherein the device described in 5) is also integrated. 2. A plate-like base (1) having projections (2) at predetermined positions on an outer periphery thereof at predetermined intervals, and at least a working electrode (2a) and a counter electrode (2) at predetermined positions of the projections (2). 2b), and a plurality of lead terminals (3a) for outputting a measurement signal of each electrode portion at a predetermined position of the base (1) facing the portion where the protruding portion is formed. Characteristic sensor device. 3. A connector (5) to which a drawer terminal (3a) is detachably connected and a drawer terminal (3a).
Amplifier for amplifying the measurement signal taken through the amplifier (7)
The sensor device according to claim 2, further comprising a connector (5) and an amplifier (7). 4. A connector (5) for connecting a plurality of bases (1) in a parallel and releasable manner to each other, and an amplifier (7) for a lead-out terminal (3a) of the plurality of bases (1). The sensor device according to claim 3, wherein the sensor device amplifies the measurement signals extracted through the sensor. 5. An electrode section having a plurality of sample storage chambers (4a) and having at least a working electrode (2a) and a counter electrode (2b) at the bottom of each sample storage chamber (4a). And a plurality of lead terminals for outputting a measurement signal of each electrode unit are also integrated. 6. A plurality of rows and a plurality of columns of projections (3) projecting at a predetermined angle from one surface of a flat substrate (31).
2), and at least a working electrode (33) and a counter electrode (34) at a predetermined position of the protruding portion (32), and a measurement signal of each electrode portion is provided at a predetermined position of an edge of the base (31). A sensor device comprising a plurality of output terminals (35) for outputting. 7. A plurality of sample storage chambers (4a), and at least a working electrode (2) is provided on an inner bottom surface of each sample storage chamber (4a).
a) and an electrode having a counter electrode (2b) are formed, and a plurality of lead-out terminals for outputting a measurement signal of each electrode are provided so as to be exposed on the bottom outer surface of each sample accommodating chamber (4a). A sensor device characterized in that: 8. A plurality of sample storage chambers (4a), each having an inward projection (84) extending inward from a central portion of the inner peripheral surface of each sample storage chamber (4a), and at least inwardly. An electrode portion is formed at a predetermined position on the outer surface of the projection (84),
The electrode section includes at least a working electrode (2a) and a counter electrode (2a).
b) and a plurality of extraction terminals for outputting a measurement signal of each electrode portion are provided so as to be exposed on the outer surface of the bottom of each sample storage chamber (4a). 9. An inward projection (84) is provided in each of the sample storage chambers (4).
Shaft (84) extending upward from the center of the bottom inner surface of a)
A working electrode (2a) and a reference electrode (2c) on the outer surface of the shaft portion (84), and a counter electrode (2
The sensor device according to claim 8, wherein an electrode portion having b) is formed.