JP2000019147A - Reaction product measuring device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To accurately identify a large number of object detecting parts by providing an identification part discretely divided for identifying the object detecting parts, and a connector for recognizing it. SOLUTION: A common terminal part 105 and a terminal part 104 connected to carbon electrodes 101, 102 of an object detecting part 100 are electrically connected to contact pins 211, 212 for detected result of a connector part 210, and sections 103A-103D of a lot discriminating electrode 103 are electrically connected to identification data reading contact pins 213-216. The detected result of an enzime reaction part 106 from the contact pins 211, 212, and an identification pattern of the lot discriminating electrode 103 from the contact pins 213-216 are respectively inputted to an arithmetic processing part 220. The arithmetic processing part 220 selects previously inputted lot-by-lot correction data of the object detecting part 100 from the inputted, identification pattern, then performs requested operation on the basis of the inputted detected result and makes lot-by-lot correction, and outputs the result to a display part 228.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a reaction product measuring device such as a biosensor. More specifically, the present invention relates to a reaction product measurement device in which a reaction product detection unit is replaceable in a reaction product measurement device in which a signal processing unit and a reaction generation processing unit are separated.

[0002]

2. Description of the Related Art A biosensor will be described as an example of a reaction product measuring device. Further, an enzyme sensor will be described as an example of a biosensor. An enzyme sensor is composed of a membrane on which an enzyme is immobilized, and an electrochemical electrode for detecting a substance produced by or catalyzed by the enzyme.

[0003] Such a biosensor has a configuration in which an object detection unit and a signal processing unit that performs various signal processing on a signal detected by the object detection unit and outputs a final measurement result are used. Have been tried. According to such a method, when a biosensor such as an enzyme sensor is used in a disposable (disposable) method, the signal processing unit can be continuously used only by replacing only the object detection unit. It is economical. However, in such a biosensor, the characteristics of the object detection unit may vary slightly for each manufacturing lot or each object detection unit. Accordingly, in the signal processing unit, if the same processing is performed on the detection signals output from all of the object detection units, an error may occur in the detection result.

[0004] As a method of overcoming such a problem,
The applicant of the present application has disclosed, for example, “Biosensor”,
As described in -160403, an electrode having a different shape is attached to each lot on the object detecting portion of the biosensor,
Also, it has been proposed that the position of the electrode is changed for each lot, the shape and arrangement position of the electrodes are detected on the signal processing unit side to identify the lot, and the signal processing is corrected for each lot.

[0005]

According to the method proposed in the above-mentioned "Biosensor", Japanese Patent Application No. 9-160403,
Even if variations occur for each lot, signal processing that corrects variations for each lot is possible. However, in the method proposed in Japanese Patent Application No. 9-160403, there is a limitation that the sensor is attached to a dimensionally limited place such as a biosensor. Furthermore, the shape of the electrodes arranged in a limited place of the connection between the object detection unit and the signal processing unit is limited, and the arrangement position of the electrodes is also limited. Therefore, there is a problem that it is difficult to identify a large number of lots due to such restrictions. Further, in the method proposed in the above-mentioned "biosensor", Japanese Patent Application No. 9-160403,
Since the identification is performed based on the mounting position of the identification electrode, if the installation position of the identification electrode is slightly different, the lot may be identified as a different lot. Conversely, in a biosensor having a limited area, it is difficult to mount electrodes with high accuracy.

SUMMARY OF THE INVENTION The present invention has been made to solve the above problems, and has as its object to provide a reaction product measuring device such as a biosensor capable of accurately identifying various lots.

[0007]

According to the present invention, there is provided an object detecting section for detecting and reacting a state of an object to be measured, and a signal for processing a signal from the object detecting section to output a detection result. In the reaction product measurement device having a processing unit, wherein the object detection unit and the signal processing unit can be connected or separated via a connector, the connection unit of the object detection unit is discretely divided A reaction product measurement device is provided, which has an identification unit for identifying the object detection unit, and wherein the signal processing unit has a connector capable of recognizing the discrete identification unit.

Preferably, a part for reading out a detection signal from the object detection part and the identification part are integrally formed.

In the present invention, since the object detecting section is indicated by a discrete identification method, the identification method of the identification section and its recognition become accurate. In other words, there is no restriction such as the mounting position of the identification electrode. Furthermore, since it is a discrete expression, it is possible to accurately identify a large number of target object detection units.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION 1
A biosensor, in particular, an enzyme sensor will be described as an embodiment. 1 and 2 are configuration diagrams of the enzyme sensor.
FIG. 1A is a plan view showing a state where an object detection unit 100 and a signal processing unit 200 of the enzyme sensor are combined, and FIG. 1B is a portion along line X1-X1 in FIG. It is sectional drawing. FIG. 2 shows that the object detection unit 100 is fitted into the connection unit (connector unit) 210 of the signal processing unit 200,
FIG. 2 is a diagram showing a state before the state of mounting shown in FIG.

The illustrated enzyme sensor comprises an object detection section 100 on which a detection element main body (sensor main body) is mounted, and a signal processing section 200. The object detection section 100 and the signal processing section 200 of the enzyme sensor are detachable, and the object detection section 100 is attached to the connection section (connector section) 210 of the signal processing section 200 as illustrated in FIG. To use.

Preferably, the connection between the connection section (connector section) 210 of the signal processing section 200 and the object detection section 100 is performed by connecting the connection section (connector section) of the signal processing section 200 as illustrated in FIG. ) The object detection unit 100 is inserted and fixed in 210, and when the object detection unit 100 is inserted in the connection unit (connector unit) 210, the discriminating electrode 103, the terminal part 104, and the common of the object detection unit 100 are connected. Terminal part 105
Are the pins 212 and 213 of the connection part (connector part) 210
It is preferable that the pins 212, 213 to 216 are formed of a resilient electric material so that the pins 212, 213 to 216 are electrically contacted with the resiliently. FIG. 3 illustrates the pin 213 as a representative for simplification of the illustration, and the other pins 211, 212, 214 to 216 are provided at the respective positions in the same manner as the pin 213. I do.
The object detection unit 100 may be inserted and removed from the connection unit (connector unit) 210 of the signal processing unit 200. In other words, the connection section (connector section) 210 of the signal processing section 200 has a structure in which the disposable (disposable) object detection section 100 can be easily inserted and removed.

The object detecting section 100 of the enzyme sensor will be described with reference to FIGS. 1 (A), 2, 3, 4 and 5. FIG. An enzyme sensor is composed of a membrane on which an enzyme is immobilized, and an electrochemical electrode for detecting a substance produced by or catalyzed by the enzyme. When such an enzyme sensor is used as a disposable (disposable), it is necessary to replace the object detection unit.

FIG. 4 shows the object detection unit 100 illustrated in FIG.
FIG. 2 is an exploded perspective view of FIG. FIG. 5 shows the object detecting unit 10.
FIG. 4 is a cross-sectional view of a lot identification electrode 103 of FIG. FIG. 6 is a cross-sectional view taken along line X2-X2 in FIG. 1A, and shows the enzyme reaction portion 106 and the carbon electrodes 101 and 1 illustrated in FIG.
02 is a diagram illustrating a cross section of an opening 108 and an insulating film 107.

The object detecting section 100 of the enzyme sensor includes a substrate 110, a first carbon electrode 101 formed on the substrate 110, and a second carbon electrode 102 facing the first carbon electrode 101. An enzyme reaction portion 106 formed in a gap between the first carbon electrode 101 and the second carbon electrode 102, and an enzyme reaction portion 106
An opening 108 is provided in a portion corresponding to the first carbon electrode 101 and an insulating film 107 partially surrounding the first carbon electrode 101 and the second carbon electrode 102. These are main parts of the object detection unit 100, and detect a reaction result in the enzyme reaction unit 106, which will be described in detail later.

A common terminal portion 105 extends from the first carbon electrode 101.
Is provided with a lot discrimination electrode 103 for identification of the object detection unit 100. Second carbon electrode 102 provided opposite first carbon electrode 101
And a terminal portion 104 is provided.

Referring to FIG. 4, a manufacturing method of the illustrated object detecting section 100 of the enzyme sensor will be described. (A) First, a substrate 110 such as PET is prepared.
The first carbon electrode 101, the common terminal portion 105, and the lot discriminating electrode 103 are integrally printed on the substrate 10. Simultaneously with this printing, the second carbon electrode 102 and the terminal portion 104 are integrally printed. That is, in the present embodiment, the first carbon electrode 101, the common terminal portion 105, and the lot determination electrode 103 are printing electrodes. Similarly, the second carbon electrode 102 and the terminal portion 104 are also printing electrodes. A first carbon electrode 101,
Since the common terminal portion 105 and the lot discriminating electrode 103 are integrally formed, it can be manufactured in a short time. Similarly,
Since the second carbon electrode 102 and the terminal portion 104 are integrally formed, it can be manufactured in a short time. Furthermore, the first
Printing of the carbon electrode 101, the common terminal portion 105 and the lot identification electrode 103, and the second carbon electrode 1
Since the printing of 02 and the terminal portion 104 can be performed in the same process, the manufacturing time can be further reduced.

(B) Next, the lot discriminating electrode 103 is divided, and unnecessary portions are cut. In the present embodiment, the lot discrimination electrode 103 is divided into four sections, and each section is a power of 2 (power).
Expressed by Then, 0- (2 ⁴ -1) = 0-1.
Six types of expressions are possible. For example, as shown in FIG. 4, if the lower one digit is cut, as shown in binary notation, it becomes "1,0,0,0" or "0,0,0,1". It becomes a numerical value meaning "8" or "1". In the present embodiment, it means "1" in hexadecimal. If this numerical value is associated with the lot number or the serial number of the object detection unit 100, it can be used for identifying the object detection unit 100. FIG. 5 illustrates a cross-sectional view of the pattern-cut lot discriminating electrode 103 portion. In the present embodiment,
Since the lot discriminating electrode 103 is discretely divided by a notation method such as a power of two, the identification method becomes accurate and easy. As described later, the lot discriminating electrode 1
The identification of the identification data reading contact pins 213 to 216 for identifying the identification number 03 is also accurate and easy.

(C) Enzyme is applied on the opposed first carbon electrode 101 and second carbon electrode 102 formed by printing the above-mentioned electrodes on the substrate 110 to form the enzyme reaction portion 106. I do.

(D) An opening 108 is provided so that the first carbon electrode 101 and the second carbon electrode 102, and the enzyme applied to these opposed electrode portions are exposed so that the detection target can contact the enzyme. Terminal portion 104 with the insulating film 107
And the common terminal portion 105. This state is shown as a sectional view in FIG.

The object detection unit 100 manufactured as described above is mass-produced for each lot, for example, and the characteristics of the object detection unit 100 of the lot are obtained as representative characteristics by sampling inspection or the like. You. Object detector 1
In particular, the characteristics of the enzyme reaction portion 106 may vary slightly from lot to lot. Therefore, if the object detection unit 100 manufactured in a lot is sampled and inspected and its variation data is collected in advance, it can be corrected when the signal processing unit 200 performs signal processing.

The object detection unit 100 manufactured as described above is used as a disposable (disposable) sensor. In the present invention, such an object detection unit 100 that cannot be used continuously is replaced to be usable, while the signal processing unit 200 is continuously usable.

The signal processing unit 200 FIG. 1 (A), the as illustrated in (B) and FIG. 2, the signal processing unit 200 of the enzyme sensor is mounted on the substrate 205, a connection portion (connector portion) 210, Arithmetic processing unit 220
And a resistor group 230.

The connector section 210 includes the object detection section 100
When the object detection unit 100
Contact pins 211 and 21 for contacting (contacting) the common terminal portion 105 and the terminal portion 104
2 and identification data reading contact pins 213 to 216 that come into contact with the respective sections of the lot identification electrode 103 of the object detection unit 100. In the example illustrated in FIG. 1B, when the common terminal portion 105 and the lot identification electrode 103 are printed on the upper surface of the substrate 110, the common terminal portion 105 and the lot identification electrode 103 can be in contact with the terminal portion 104, the common terminal portion 105 and the lot identification electrode 103. In addition, contact pins 211 and 212 for detection result and contact pins 213 to 216 for reading identification data are provided on the lower surface of the substrate 205. The detection result contact pins 211, 212
And contact pin 213-2 for reading identification data
16 may be a conductive member, and need not be the same as the carbon electrode in the object detection unit 100. As the detection result contact pins 211 and 212 and the identification data reading contact pins 213 to 216, for example, a conductive metal such as aluminum, copper, or gold is used for the substrate 2.
05 can be formed by printing. Of course, the detection result contact pins 211 and 212 and the identification data reading contact pins 213 to 216 can be carbon-printed.

The resistor group 230 includes a plurality of resistors used for pull-up or pull-down.

The arithmetic processing unit 220 includes a current-voltage conversion circuit 2
22, amplifying circuit 224, arithmetic unit 226 and display unit 22
8 The current-voltage conversion circuit 222 reads out the detection result contact pins 211 and 212 and converts the detection signal (current signal) in the enzyme reaction portion 106 into a voltage signal, and amplifies the voltage in the amplification circuit 224.
In 6, various calculations are performed, and the results are displayed on the display unit 228. In order to perform various calculations, the calculation unit 226 performs calculations using a microcomputer, for example. As the display portion 228, various displays such as a liquid crystal display, a CRT display, and a plasma display can be used. In addition, as a device for displaying the result of the calculation unit 226, a printing device such as a printer device can be provided in addition to the display unit 228. Further, if necessary, the calculation result of the calculation unit 226 can be transmitted to a remote device by data transmission or the like.

The object detection unit 100 and the signal processing unit 200
The object detection unit 100 and the signal processing unit 200 described above are prepared, and as illustrated in FIG. 2, the lot detection electrode 103 portion of the object detection unit 100 and the signal processing unit 200 The common terminal portion 105 connected to the first carbon electrode 101 and the terminal portion 104 connected to the second carbon electrode 102 are electrically connected to the contact pins 211 and 212 for detection results. To the lot identification electrode 103,
3D is a contact pin 213 to 2 for reading identification data.
16 is electrically connected.

As a result, in the arithmetic processing section 220 of the signal processing section 200, the detection result contact pins 211, 2
12, the detection result of the enzyme reaction portion 106 and the pattern of the lot discrimination electrode 103 from the identification data reading contact pins 213 to 216 are input. First, the arithmetic processing unit 220 reads the identification pattern of the lot identification electrode 103 from the identification data reading contact pins 213 to 216, and selects correction data for each lot of the object detection unit 100 that has been input in advance. In addition,
As the correction method for each lot, correction data for each lot is stored in advance in the calculation unit 226, and the lot number indicated by the lot discrimination electrode 103 is identified, and the corresponding correction data is selected and used. be able to. After that, the arithmetic processing unit 220 starts the enzyme reaction part 1 of the object detection unit 100.
The result of the detection is read, the desired calculation processing is performed, the above-described correction is performed for each lot, and the result is output to the display unit 228. Note that the arithmetic processing itself in the arithmetic processing unit 220 is the same as a known method, and a detailed description thereof will be omitted.

Replacement of the Object Detecting Unit 100 When replacing the object detecting unit 100 with a new one, the previously used object detecting unit inserted and connected to the connection unit (connector unit) 210 of the signal processing unit 200 is detected. Remove the part 100. Then, the new object detection unit 100 is connected to the signal processing unit 2.
In the same manner as described above, the replacement detection unit 100 is attached to the connection unit (connector unit) 210, and the replaced object detection unit 100 is identified. If necessary, correction is performed based on the identification result, and the actual detection processing is performed. Do.

In the above-described embodiment, the lot discriminating electrode 103 of the object detection unit 100 is divided into four sections.
Although the lot number and the like can be displayed in 15 16 different numerical values (codes), the present invention is not limited to dividing the lot discriminating electrode 103 into four, and may be divided into an arbitrary number. . For example, the lot number can be divided into three, and in that case, the lot number can be represented by eight numerical values from 0 to 7. In this case, the number of contact pins for reading identification data in the signal processing unit 200 may be three. Conversely, the lot discrimination electrode 103 is divided into 5
The street lot number can also be displayed. Further, the lot discrimination electrode 103 is not limited to the above-described embodiment, and can take various modifications. For example, the division is not limited to the power of 2 display, but also the decimal display,
Alternatively, each section may correspond to an alphabet. Further, the lot discriminating electrode 103 can be used for a correction data number instead of a lot number. That is, by representing the lot discriminating electrode 103 in various discrete forms, the object detection unit 100
Can be used for accurate identification of The lot discriminating electrode 103 of the present embodiment is distinct because the division is discrete, and the identification data reading contact pins 213 to 216 corresponding thereto are also accurately determined, so that the object detection is performed. The identification of the unit 100 is also accurate. Further, by combining the lot identification electrode 103 with the identification data reading contact pins 213 to 216, the identification of the object detection unit 100 is automatically performed, and the subsequent processing, for example, correction of calculation or measurement result is performed. It can be used for organizing, etc.

The lot discriminating electrode 1 of the object detecting section 100
03, the electrical connection between the terminal portion 104 and the common terminal portion 105 and the detection result contact pins 211 and 212 and the identification data reading contact pins 213 to 216 in the connector portion 210 of the signal processing section 200 is limited to the above-described method. Instead, various modes can be adopted. For example, detection result contact pins 211 and 21
2 and contact pins 213 to 21 for reading identification data
6 are arranged in a line, and the detection result contact pins 211 and 212 are also arranged in a line on the object detection unit 100 side.
And contact pins 213 to 216 for reading identification data
To match the shape.

The object detection unit 100 and the signal processing unit 2
00 is connected to the connector unit 210 in the signal processing unit 2.
The connector section 210 of the object detection section 100 can be formed as a female connector, and the lot identification electrode 103, the terminal section 104, and the common terminal section 105 of the object detection section 100 can be formed into a male type so as to fit each other. Also, the reverse can be done.

As described above, as an embodiment of the reaction product measuring apparatus of the present invention, a biosensor, in particular, an enzyme sensor has been exemplified. However, it is needless to say that the present invention can be applied to other biosensors. It is needless to say that the present invention can be applied to various reaction product measuring devices.

[0034]

According to the present invention, since the object detecting section is indicated by a discrete identification method, the identification method of the identification section and its recognition become accurate.

Further, in the present invention, since the detection electrode of the reaction part and the identification part are integrated, the configuration is simple and the production can be facilitated.

[Brief description of the drawings]

FIGS. 1A and 1B are configuration diagrams of an enzyme sensor as an embodiment of a reaction product measurement device of the present invention, and FIG. FIG. 2 is a plan view showing a state where the signal processing unit of the enzyme sensor is combined with the signal processing unit, and FIG.
FIG. 2B is a partial sectional view taken along line X1-X1 in FIG.

FIG. 2 is a diagram illustrating an object detection unit of the enzyme sensor illustrated in FIGS. 1A and 1B and a connection unit (connector unit) of a signal processing unit;
FIG. 2 is a view showing a state before being fitted into the mounting state shown in FIG.

FIG. 3 is a diagram illustrating an insertion / connection state between a connection section (connector section) of the signal processing section illustrated in FIG. 1 and an object detection section;
FIG.

FIG. 4 is an exploded perspective view of the object detection unit illustrated in FIG. 2;

FIG. 5 is a cross-sectional view of a lot discriminating electrode of the object detection unit.

FIG. 6 is a cross-sectional view taken along line X2-X2 in FIG. 1 (A), and a cross-sectional view of the enzyme reaction portion illustrated in FIG. It is.

[Explanation of symbols]

100 object detection unit 101 first carbon electrode 102 second carbon electrode 103 electrode for determination 104 terminal part 105 common terminal part 106 enzyme reaction part 107 insulation Film 108, opening 110, substrate 200, signal processing unit 205, substrate 210, connection unit (connector unit) 211, 212, contact pins for detection results 213 to 216, contact pins for reading identification data 220 ..Arithmetic processing section 222..current-voltage conversion circuit 224..amplifier circuit 226 ... arithmetic section 228..display section 230 .. resistor group

Claims (2)

[Claims] An object detection unit for reacting and detecting a state of a measurement object, and a signal processing unit for performing signal processing on a signal from the object detection unit and outputting a detection result, wherein the object In a reaction product measurement device in which a detection unit and the signal processing unit can be connected or separated via a connector, a connection unit of the object detection unit is for identifying the object detection unit that is discretely divided. A reaction product measuring device, comprising: an identification unit; and the signal processing unit includes a connector capable of recognizing the discrete identification unit. 2. The apparatus according to claim 1, wherein a portion for reading out a detection signal from the object detection unit and the identification unit are integrated.
The reaction product measuring device according to the above.