JPH10262607A - Nutrient composition for infant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject composition having promoting action on maturity of digestive tract by including RNA and a polyamine. SOLUTION: This composition contains preferably 1-200 mg of RNA based on 100 g solid of the composition and preferably 10-2,000 μg of a polyamine. The polyamine is preferably at least two or more selected from the group consisting of spermine, spermidine, putrescine and cadaverine. Preferably the composition comprises 10-90% of spermine, 10-90% of spermidine, 0-90% of putrescine and 0-20% of cadaverine.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a nutritional composition for infants containing RNA and polyamine and having a digestive tract maturation promoting action. By ingesting the nutritional composition for infants of the present invention, the maturation and development of the immature digestive tract during infancy can be promoted, and the digestive and absorptive ability can be improved, which is useful as a medicine or food.

[0002]

2. Description of the Related Art RNA is a polynucleotide in which nucleotides containing base components such as adenine and cytosine form a single linear polymer through phosphodiester bonds,
It is involved in protein synthesis in all cells in the body. RNA is also contained in breast milk at high concentrations, and its physiological role in infants is of interest. It is thought that RNA taken orally is decomposed into nucleotides and nucleosides and absorbed by the action of nucleases and phosphatases in the digestive tract. Therefore, as for the physiological action of RNA, an immunostimulatory action, an improvement and promotion of lipid metabolism, and an action of improving brain function can be expected as in the case of nucleotides. ADKulkarni et al. Report that raising mice on a diet supplemented with RNA increases resistance to bacterial infection and improves immunity (Journal of
Parenteral and Enteral Nutrition, 10, 169-171 (198
6)). Hamada et al. Show that feed supplemented with RNA suppresses diarrhea in young animals and promotes growth (Japanese Patent Application Laid-Open No. 43-28059). Despite having such an effect, the conventional infant nutrition composition using milk as a main ingredient hardly contained RNA. In addition, in conventional infant formulas, those containing nucleotides were also found, but infants taking breast milk also take RNA as well as nucleotides and take artificial milk. It may be necessary to fortify RNA into milk powder in order to meet infant needs.

[0003] On the other hand, polyamines are linear aliphatic hydrocarbons having two or more primary amino groups, such as putrescine, spermidine and spermine, and have a stabilizing effect on nucleic acids.
Physiological actions such as the action of promoting nucleic acid synthesis, activating protein synthesis, the action of promoting histone acetylation and protein phosphorylation, and the action of stabilizing cell membranes are known. Also recently,
The effect of promoting cell proliferation and differentiation has also been reported. In particular, it has been reported that ingested polyamines mature the gastrointestinal mucosa (Buts JP et al., Digestive
Diseases and Science, 38, 1091 (1993); Grant, AL e
t al., J. Anim. Sci., 68, 363 (1990); Dufour, C. et a
l., Gastroenterology, 95, 112 (1988)). It has also been reported that polyamines taken orally are rapidly taken into the body and used in tissues (Bardocz, S. et al.,
J. Nutr. Biochem., 4, 66 (1993)). On the other hand, examples of application to foods include a method for producing konjac that reduces odor peculiar to konjac and has no adverse effect when cooked with other foods (JP-A-6-38690), or promotes absorption of protein. Nutritional compositions (JP-A-6-305596) for the purpose of maintaining good growth and a healthy state are known. Examples of the use of polyamines as pharmaceuticals include a method for inhibiting gastric acid secretion, a composition for ingestion for inhibiting gastric acid secretion (JP-A-58-131914), and an immunostimulant (JP-A-59-98015 and JP-A-2-223514) is known.
As described above, polyamines are known to have various effects, and since their importance has recently been recognized, it is desirable to enhance a nutritional composition having a low polyamine content from a nutritional point of view. Conceivable. However, similarly to RNA, the nutritional composition containing milk as a main ingredient contained almost no polyamine.

[0004] In recent years, infant formula has been improved due to improvements in technology.
Compared to the ultimate goal of breast milk, products are offered that are fairly similar in composition. However, in terms of functionality, there are still many problems compared to breast milk,
Formulated milk powder more similar to breast milk is desired. Conventional infant formulas lacked the proper ingredients and did not fully recognize the need for RNA or polyamines during infancy, so these components were simultaneously formulated or fortified in the nutritional composition for infants. I never did.

[0005]

In view of the situation described above, the present inventors have conducted intensive studies on polyamines or RNAs and found that by adding both substances within a specific range, the digestive tract of infants can be matured. Or they found that they significantly promoted development. In addition, the present inventors have conducted intensive studies on the problems of conventional infant formulas, and as a result, by adding polyamine and RNA at the same time, have come to develop a milk formula that is more similar in function to mother's milk. Therefore, an object of the present invention is to provide a nutritional composition for infants, which has an action of promoting digestive tract maturation, containing RNA and a polyamine.

[0006]

SUMMARY OF THE INVENTION The present invention relates to a nutritional composition for infants containing RNA and a polyamine and having a digestive tract maturation promoting action. Specifically, the composition solid 10
The present invention relates to a nutritional composition for infants, which comprises 1 mg to 200 mg of RNA and 0 μg to 2000 μg of polyamine per 0 g. At this time, as the polyamine, at least two or more selected from the group consisting of spermine, spermidine, putrescine, and cadaverine are used, and 10-90% of spermine and 1 of spermidine.
0-90%, putrescine 0-90%, and / or cadaverine 0-20%. By ingesting the nutritional composition for infants of the present invention, maturation and development of the immature gastrointestinal tract during infancy can be promoted, and the digestive and absorptive ability can be improved, which is useful as a medicine or food.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The RNA and polyamine of the present invention are used in an amount of 1 mg to 100 mg of RNA per 100 g of solid composition.
It is characterized by containing 200 mg and 10 μg to 2000 μg of polyamine. At this time, if the respective components are less than these amounts, no effect will be obtained, and since the contents in breast milk are respectively about this level, it is not preferable to further contain them from the viewpoint of approximation to breast milk. As the polyamine in the present invention, at least two or more selected from the group consisting of spermine, spermidine, putrescine, and cadaverine are used. Particularly, it is preferable to mix three kinds of spermine, spermidine and putrescine.
The composition ratio of the polyamine is 10-90% for spermine, 10-90% for spermidine, and 0-90 for putrescine.
% And / or 0 to 20% of cadaverine. The RNA source used in the present invention is not particularly limited as long as it is used as a food. Preference is given to milt or yeast of fish. The polyamine source to be added is not particularly limited as long as it is used similarly as food. For example, animal organs or milts can be mentioned.

The nutritional composition for infants according to the present invention includes infant formulas, infant formulas for low birth weight infants, follow-up milks, infant formulas for allergic diseases, etc., and includes proteins, lipids, carbohydrates, vitamins and the like. It is composed mainly of minerals. In addition, you may contain the component which can be further mix | blended with the nutrition composition for infants.
Protein sources include casein, whey protein concentrate (WP
C), whey protein isolate (WPI), αs-casein, β
-Milk protein fractions such as casein, α-lactalbumin, and β-lactoglobulin, or plant proteins such as soy protein and wheat protein, and peptides or amino acids obtained by enzymatically decomposing these to various degrees of degradation. Etc. can be used. As fat sources, milk fat, lard, tallow, or animal fats such as fish oil including bonito oil, tuna oil, or soybean oil, rapeseed oil, corn oil, coconut oil, palm oil, palm kernel oil, safflower oil ,
Vegetable oils such as perilla oil, linseed oil, evening primrose oil, MCT, and cottonseed oil, as well as fractionated oils, hydrogenated oils, and transesterified oils thereof can be used. Lactose, maltose, glucose, sucrose, dextrin,
Oligosaccharides such as 6'-galactosyl lactose, fructooligosaccharides, lactulose, artificial sweeteners, soluble polysaccharides, starch and the like can be used. Sources of vitamins and minerals include: “CODEX standards and special sanitary work regulations for special purpose foods, including infant foods, CAC / VOL.IX-
1st Edition and Supplement 1,2,3,4 ”(published by Japan Federation of Dairy Agriculture, 1993),“ Handbook of Designated Food Additives 1993 Edition (revised 31st edition) ”published by Food and Science Co., 1993
) Or "Notification of Food Additives / Food Material Natural Products Handbook (Twelfth Edition)" (published by Food and Science Co., Ltd., 1992). Can be used. Examples of vitamins include vitamins A, B, C, D, E, K, folic acid,
Pantothenic acid, β-carotene, nicotinamide and the like, and as minerals calcium, magnesium, iron, copper, zinc, iodine, manganese,
Selenium and the like. The mixing ratio of protein, fat, carbohydrate, vitamin, and mineral is 5 to 5 per solid.
50% by weight, 5 to 50% by weight, 20 to 80% by weight, 0.
Preferably, the content is 005 to 5% by weight and 0.005 to 5% by weight.

[0009] The nutritional composition thus obtained is capable of increasing the function of digestion and absorption of nutrients, inhibiting the permeation of allergens, expressing various functions inherent in the gastrointestinal tract, and the activity of natural killer cells and macrophages. Stimulatory effect such as increase of lipase, activation of desaturase, D in brain
Effects such as an increase in the amount of HA or arachidonic acid or an increase in in vivo phospholipids can be expected. Furthermore, the effect of simultaneous contact of RNA and polyamine can be further enhanced by other substances such as arginine, β-carotene, ganglioside, sialic acid, lactoferrin, arachidonic acid, DHA, and the like.

[0010]

The present invention will be described in more detail with reference to the following examples, which are merely illustrative and do not limit the present invention in any way.

[0011]

[Example 1] Preparation of powdered milk containing RNA and polyamine 1) 96 kg of skim milk, 3 kg of whey protein concentrate (WPC; Denmark Protein) and 17.6 kg of lactose were added and dissolved, and water-soluble vitamin components (vitamin B1, B
6, B12, C, niacin, folic acid, pantothenic acid, biotin, choline, inositol, etc.) and mineral components (calcium carbonate, potassium chloride, magnesium sulfate,
0.4 kg each of sodium ferrous citrate, copper sulfate, zinc sulfate) and one yeast RNA (about 50% purity)
00g, polyamine preparation method (JP-A-8-23809)
No. 4), 36 g of a polyamine preparation prepared from salmon milt, and fat-soluble vitamins (vitamin A,
D, E, K, β-carotene, etc.), linoleic acid, γ-linolenic acid, arachidonic acid, α-linolenic acid,
Formulated fat 9.5 containing EPA (eicosapentaenoic acid), DHA (docosahexaenoic acid), and cholesterol
6 kg were mixed and homogenized. Sterilize the resulting solution,
It was concentrated by a conventional method and dried to obtain 120 kg of milk powder.
The milk powder prepared by dissolving the milk powder in warm water to a solid content of 13% contained about 5 mg / 100 ml of RNA and about 120 μg / 100 ml of polyamine.

[0012]

Example 2 Preparation of powdered milk with RNA and polyamine. 760 g of whey powder 2 and 20 g of lactose were dissolved in 3 kg of hot water, and 20 g of the same water-soluble vitamin and mineral components as in Example 1 were added thereto. About 50%)
0.2 g, polyamine preparation method (JP-A-8-2380)
No. 94), 30 mg of a polyamine preparation prepared from salmon milt, 740 g of skim milk powder, and a prepared fat 47 in which the same fat-soluble vitamin component as in Example 1 was dissolved.
8 g was added and homogenized. The obtained solution was sterilized, concentrated by a conventional method, and dried to obtain 2 kg of milk powder. RNA is contained in a product prepared by dissolving this milk powder in warm water and containing about 0.1% of RNA.
3 mg / 100 ml, polyamine is about 1.3 g / 100
ml was included.

[0013]

Example 3 Composition of Nutrient Composition for Infants and Infants of the Present Invention The following table shows typical compositions (per 100 ml) of the nutritional composition for infants in the present invention. In addition, ganglioside, arachidonic acid, lactoferrin, lactulose, galactooligosaccharide, cholesterol and the like may be added as other components not described.

[0014]

[Table 1]

[0015]

[Test Example 1] Intestinal maturation promoting effect The intestinal maturation promoting effect was tested according to the following method.
That is, according to the method of Hall et al. (Science, 109, 1313-1315, 1975), SD rats (5 days old) were cannulated and artificially reared. The formula at this time is the composition of rat milk (Dymsza et al., Journal of Nutrition, 84, 100, 1964.)
The one prepared according to the procedure was used. The feeding amount of the artificial milk was such that the artificially bred rats showed the same weight gain as the motherly bred rats. From 5 days old to 10 days old, they were nursed with the above-mentioned artificial milk. From 11 days old to 14 days old, they were divided into 4 groups, which were used as a control group and an addition group. The control group received the same artificial milk as it was, and the added group received only RNA, only polyamine, and R
An artificial milk to which each of NA and polyamine was added was given. 5 mg / 100 ml for RNA, 120 for polyamine
Added at μg / 100 ml. At the age of 15 days, the animals were sacrificed by whole blood sampling under ether anesthesia, and the small intestine was removed. Under ice temperature, the small intestinal mucosa was scraped from the small intestine with a glass cover, suspended in cold physiological saline, and then a small intestinal mucosal fraction was prepared with a homogenizer. Lactase and maltase disaccharide degrading enzyme activities in the small intestinal mucosal fraction were measured according to the method of Dahlqvist et al. (Anal. Biochem., 7, pp. 18-25 (1964)). That is, using lactose and maltose as substrates, 50 μl of a substrate solution and 50 μl of a small intestinal mucosal fraction were mixed and incubated at 37 ° C. for 30 minutes. After the incubation, 20 μl of the reaction solution was collected, and the amount of glucose released and decomposed by the enzyme in the small intestinal mucosa was measured using a glucose measurement kit (Wako Pure Chemical Industries, Ltd.). And In this method, maturation of intestinal cells can be confirmed by decreasing lactase activity and increasing maltase activity in tissues.
The results are shown in FIGS. In the group containing only RNA, there was almost no difference as compared with the control group.However, in the group to which RNA and polyamine were simultaneously added, the lactase activity, which is an indicator of maturation, was decreased and the lactase activity was lower than that in the case of adding polyamine alone. An increase in maltase activity was observed. RN
It was confirmed that the interaction between A and the polyamine synergistically promotes intestinal mucosal maturation.

[0016]

[Test Example 2] Intestinal maturation promoting effect As in Test Example 1, SD rats (5 days old) were cannulated and artificially reared. The groups were divided into control group (artificial milk administration group), RNA added group, polyamine added group, RN
A and the polyamine addition group. 2.5 mg / RNA
100 ml of polyamine was added to 60 μg / 100 ml artificial milk. The test substance was administered from the age of 5 days to the age of 14 days. At the age of 15 days, the animals were sacrificed by whole blood sampling under ether anesthesia, and the small intestine was removed and used as a specimen. Histological examination (villi height and glandular depth) was performed as well as the total wet weight of the specimens. The results are shown in FIGS. When RNA or polyamine was administered alone, there was little effect compared to the control group, but it was confirmed that simultaneous administration of RNA and polyamine significantly promotes intestinal maturation due to the synergistic action of both. Was done.

[0017]

As described above, according to the present invention, a nutritional composition for infants, which has an action of promoting digestive tract maturation, containing RNA and polyamine is provided. By ingesting the nutritional composition for infants of the present invention, maturation and development of the immature gastrointestinal tract during infancy can be promoted, and the digestive and absorptive ability can be improved, which is useful as a medicine or food.

[Brief description of the drawings]

FIG. 1 shows the lactase activity (%) in the small intestine in each administration group in Test Example 1.

FIG. 2 shows the intestinal maltase activity (%) in each administration group in Test Example 1.

FIG. 3 shows the total wet weight (mg / cm) of the small intestine in each administration group in Test Example 2.

FIG. 4 shows the villi height and glandular depth (μm) of the small intestine in each administration group in Test Example 2.

Claims (4)

[Claims] 1. A nutritional composition for infants having a digestive tract maturation promoting action, comprising RNA and a polyamine. 2. An amount of 1 RNA per 100 g of solids of the composition.
mg to 200 mg, and 10 μg to 200 mg of polyamine.
The nutritional composition for infants according to claim 1, comprising 0 µg. 3. The nutritional composition for infants according to claim 1, wherein the polyamine comprises at least two members selected from the group consisting of spermine, spermidine, putrescine, and cadaverine. 4. Spermine 10-90%, spermidine 10-90%, putrescine 0-90%, and / or
Or containing 0 to 20% cadaverine,
The nutritional composition for infants according to claim 1.