JPH10194980A - Prevention of viral infection of cultured fish, agent for preventing viral infection and use of killed bacterium cell - Google Patents

Abstract

PROBLEM TO BE SOLVED: To enable to prevent the viral infection of cultured fishes by administering killed bacterium cells to the cultured fishes. SOLUTION: This method for preventing the viral infection of cultured fishes comprises orally administering killed butyric acid bacterium cells to the cultured fishes such as sea bream, young yellowtail, USHIEBI or goldfish. The killed butyric acid bacterium cells are preferably the killed cells of Clostridium butyricum, especially the killed cells of its trophozoite, more preferably the continuously long administrable killed cells of the trophozoite of Clostridium butyricum MIYAIRI (MIYAIRI bacterium). The killed cells can be administered as such or after mixed with a formula feed. The killed cells are mixed with the formula feed in an amount of 10<5> -10<10> cells per 100g of the formula feed, and the mixed formula feed is subsequently fed at a conventional dose. When administered as such, the killed butyric acid bacterium cells are administered at a dose of 10<4> -10<10> cells per kg of the body weight of the cultured fish. The economical value for reducing an economical loss accompanied by the viral infection of the fishes is large in the fish culture industry.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to the control of virus infection in aquaculture animals and the like.

[0002]

2. Description of the Related Art It is known to administer killed cells of butyric acid bacteria to aquaculture animals for maintaining health (Japanese Patent Laid-Open No. 4-21).
7922). However, it is not known that killed cells of butyric acid bacteria are effective for controlling virus infection in aquaculture animals and the like.

[0003]

SUMMARY OF THE INVENTION An object of the present invention is to provide a method for controlling virus infection in aquaculture animals and the like.

[0004]

Means for Solving the Problems As a result of various studies, the present inventors have found that virus infection can be controlled by administering killed butyric acid bacteria to aquaculture animals, and completed the present invention.

That is, the present invention relates to (1) the use of killed butyric acid bacteria for controlling viral infection of aquaculture animals, and (2) the use of the above (1) wherein the butyric acid killed cells are dead cells of Miyairi. The use described in (3) The use described in (1) above, wherein the dead cells of butyric acid bacteria are dead cells of vegetative bodies of Mytilus bacteria, (4) Oral administration of dead cells of butyric acid bacteria to aquaculture animals (5) A method for controlling virus infection in aquacultured animals, which comprises orally administering killed butyric acid bacteria to the aquacultured animals from the age of a gifted animal. ) A method for controlling viral infection in aquaculture animals, characterized in that killed butyric acid bacteria are continuously orally administered to aquaculture animals from the time of ingestion of fry or larvae mixed feed, (7) Butyric acid bacteria in aquaculture animals A method for controlling virus infection in aquacultured animals, characterized in that killed cells are added to a compound feed and administered orally. (8)
The above (4), wherein the butyric acid bacterium dead cells are dead cells of Miyairi bacteria.
(5), the control method according to (6) or (7), (9) the above (4), (5), wherein the killed cells of butyric acid bacteria are killed cells of vegetative bodies of Miyairi bacteria
, (6) or (7), the control method described in (7), (10) an oral virus infection control agent containing butyric acid bacterium dead cells as an active ingredient,
(11) The above-mentioned (1), wherein the dead cells of butyric acid bacteria are dead cells of Miyai bacteria
The present invention relates to the pesticidal agent according to (10), wherein the killed cells of butyric acid bacteria are killed cells of vegetative bodies of Mytili bacteria.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION As the killed cells of butyric acid bacteria used in the present invention, killed cells of the species Clostridium butyricum are preferred, and particularly killed cells of the vegetative form are preferred. Among them, Clostridium butyricum miyairi ( The killed cells of M. aureus, especially the vegetative bodies of M. i. The dead cells of Miyairi fungus are disclosed in
It is a known substance disclosed in 2-17922. Dead bacteria of Miyairi bacteria may be administered as they are,
Also, rice bran oil cake, flour, glucose, lactose, silicic anhydride, calcium carbonate, bran, beer yeast, soybean cake,
It may be diluted with a suitable excipient such as soy protein. Also,
It is more effective when used in combination with vitamin C and vitamin E. When the killed cells of Miyai bacteria are used by mixing with the excipient as described above, that is, when the oral virus infection controlling agent of the present invention contains an excipient, the blending ratio of the killed bacteria of Iyairi and excipients Is preferably 1: 1 to 1000 (weight ratio), and particularly preferably 1: 5 to 500 (weight ratio).

[0007] Examples of aquacultured animals include yellowtail, amberjack, striped horse mackerel, tiger pufferfish, flounder, sea bass, eel, ayu, Thai, flounder, prawn, prawn, lobster, coho salmon, rainbow trout, goldfish, and the like. It is not limited, and may be a fry or a larva or a parent.

As a method for orally administering killed cells of butyric acid bacteria to aquacultured animals, the agent for controlling viral infection of the present invention for oral use can be administered as it is or by mixing it with a mixed feed. Even when aquacultured animals are continuously orally administered with butyric acid bacteria-killed bacterial cells from the age of the animal, for example, from the ingestion of fry or larvae compound feed, the virus infection controlling agent of the present invention may be fed as it is. Alternatively, the killed cells of butyric acid bacteria may be added to a mixed feed for juveniles or larvae or a normal mixed feed, and the mixture may be supplied.

The dose of killed butyric acid bacteria to aquacultured animals is not particularly limited, but usually 10 ⁵ to 10 ¹⁰ , preferably 10 ⁶ to 10 ⁹ killed butyric acid bacteria per 100 g of the mixed feed. May be added and mixed and fed in the usual amount to be fed. When the virus infection controlling agent is administered as it is, 10 ⁴ to 10 per 1 kg of body weight of aquacultured animals.
^It is sufficient to administer 10, preferably 10 ⁵ to 10 ⁹ .

[0010] The administration period is not particularly limited, and is usually one week or more. When administration is performed from a gifted animal, it is desirable that administration be continued until the product is shipped. In addition, when administered to parents, it is sufficient to administer for about one month before spawning,
There is no problem with further administration. The administration may be performed daily, but may be performed at intervals of 1 to 10 days.

The oral virus infection controlling agent of the present invention is particularly effective for controlling virus infection in aquaculture animals.
It can also be used to control viral infection of other animals such as cattle, pigs, chickens, dogs, cats and the like.

[0012] Oral administration of killed butyric acid bacteria to aquacultured animals and the like can prevent infection of various viruses such as iridovirus, baculovirus, herpes virus, FHV virus and PAV virus.

[0013]

The present invention will be described below in detail with reference to examples, but the present invention is not limited to these examples. Example 1 Goldfish one month after hatching, which was fed continuously for 2 to 5 months every day at 5% of the body weight of a goldfish with a mixed feed containing 10 ⁸ killed cells of the vegetative body of the filial fungus per 100 g of the mixed feed A two-year-old goldfish "Ranchu" was fed 200 times, and as a control, killed cells of Miyai were added at a rate of 10 ⁸ per 100 g of the mixed feed, and the mixed feed was fed continuously for 6 months at 5% of the body weight of the goldfish. Ten ranchus were allowed to live with herpesvirus poisoned fish for one day. Results The experimental results are as shown in the table, and the yearling fish to which the killed cells of Miyai bacteria were continuously administered daily were not infected with the virus at all. On the other hand, all of the two-year-old fish to which dead bacteria of Mytilus bacillus were administered every day died within 1 week due to virus infection and could not control virus infection at all. The mortality rate was 40% in the case of the year-old fish to which the miyairi powder was not administered.

[0014]

[Table 1] By χ ² independence test; ^** significant difference compared with those old fish MIYAIRI bacteria the end of the group administered (P <0.01).

Example 2 1 g of dead cells of a vegetative body of Miyai bacterium using lactose as an excipient
A preparation containing 10 ⁸ per was prepared and dissolved as it was at a concentration of 1 ppm in the water of a bovine shrimp seedling raising tank, and fed to larval shrimp. As for the number of times of feeding, feeding was performed four times in 20 days from the time when the seedlings were introduced into the aquarium to the time when the shrimp seedlings were shipped. Results The experimental results are as shown in Table 2, and the viability of the vegetative body of the slime mold increased the viability of the shrimp shrimp seedlings.

[0016]

[Table 2]

[0017] Example 3 dead cells of scampi feed 100g nutrition of MIYAIRI bacteria was added to a 10 ^7, and 21 days continuously fed to a parent scampi. Thereafter, the shrimp were taken out and spawned to obtain larval hand shrimp. The larvae were bred for one month in a nursery tank and then shipped. During the breeding period in the seedling aquarium for one month, a formulation containing 10 ⁸ dead cells per gram of a vegetative body of Miyai bacteria using lactose as an excipient at a concentration of 1 ppm in the seedling water once a week. And were fed to larval shrimp. Results The experimental results are as shown in Table 3, and the survival rate was increased by the administration of the killed cells of the vegetative body of Miyai bacteria to the parent shrimp and the larvae of the lobster. On the other hand, the non-administration group was extinct.

[0018]

[Table 3]

[0019]

As is apparent from the above description, the present invention makes it possible for the first time to apply butyric acid-killed cells to the control of virus infection in aquaculture animals, and to reduce the economic loss associated with the control of virus infection in the aquaculture industry. The mitigation value is great.

Claims (12)

[Claims] 1. Use of killed butyric acid bacteria for controlling viral infection of aquaculture animals. 2. The use according to claim 1, wherein the killed cells of butyric acid bacteria are dead cells of Miyai bacteria. 3. The use according to claim 1, wherein the killed cells of butyric acid bacteria are killed cells of vegetative bodies of Mytilus. 4. A method for controlling viral infection of aquaculture animals, which comprises orally administering killed cells of butyric acid bacteria to the aquaculture animals. 5. A method for controlling viral infection of aquaculture animals, which comprises orally administering killed cells of butyric acid bacterium to aquaculture animals from the age of a talented animal. 6. A method for controlling viral infection of aquacultured animals, comprising continuously orally administering killed butyric acid bacteria to the aquacultured animals from the time of feeding the fry or larvae compound feed. 7. A method for controlling virus infection of aquacultured animals, wherein killed cells of butyric acid bacteria are added to the formulated feed and administered orally to the aquacultured animals. 8. The control method according to claim 4, wherein the killed cells of butyric acid bacteria are killed cells of Miyai bacteria. 9. The control method according to claim 4, wherein the killed cells of butyric acid bacteria are killed cells of vegetative bodies of Mytilus. 10. An oral virus infection controlling agent containing dead cells of butyric acid bacteria as an active ingredient. 11. The control agent according to claim 10, wherein the killed cells of butyric acid bacteria are killed cells of Miyai bacteria. 12. The control agent according to claim 10, wherein the killed cells of butyric acid bacteria are killed cells of vegetative bodies of Mytili bacteria.