JP2558715B2 - How to rear young larvae - Google Patents
How to rear young larvaeInfo
- Publication number
- JP2558715B2 JP2558715B2 JP62170505A JP17050587A JP2558715B2 JP 2558715 B2 JP2558715 B2 JP 2558715B2 JP 62170505 A JP62170505 A JP 62170505A JP 17050587 A JP17050587 A JP 17050587A JP 2558715 B2 JP2558715 B2 JP 2558715B2
- Authority
- JP
- Japan
- Prior art keywords
- fish
- growth hormone
- substance
- organism
- juvenile
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Feed For Specific Animals (AREA)
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、仔稚幼魚の飼育方法に関するもので、本発
明の仔稚幼魚の飼育方法は、魚類の増養殖産業におい
て、特に種苗生産においての利用が期待される。TECHNICAL FIELD The present invention relates to a method for raising juveniles and juveniles, and the method for raising juveniles and juveniles of the present invention is used in the fish aquaculture industry, particularly in seedling production. Is expected to be used.
魚類の増養殖産業における特に重要な問題は、いかに
して健全な種苗を大量に且つ安定して入手するかにあ
る。この種苗の入手方法としては、天然種苗を採捕す
る方法及び人工種苗を生産する方法の２方法がある。
上記の方法は、従来、サケ、マス、タイ、クルマエビ
等においては確立されているが、海産魚類においては、
シマアジ、ハタ、クエ、マグロ等、いまだに確立されて
いない魚種も多い。A particularly important issue in the fish aquaculture industry is how to obtain healthy seeds in large quantities and stably. There are two methods for obtaining these seedlings: a method for collecting natural seedlings and a method for producing artificial seedlings.
The above method has been conventionally established in salmon, trout, Thailand, prawns, etc., but in marine fish,
There are many fish species that have not yet been established, such as striped horse mackerel, grouper, queer, and tuna.
この原因は、成熟した卵の入手が困難な事と、孵化仔
魚を幼魚にまで成長させる間の大量斃死の問題が存在す
る事にある。This is due to the difficulty in obtaining mature eggs and the problem of mass mortality during the growth of hatched larvae into juveniles.
また、孵化仔魚が幼魚にまで成長する間の餌料は、サ
ケ、マス等の淡水魚については、人工餌料が開発されて
いるのに対し、海産魚については、孵化後の消化器官の
発生が淡水魚とは異なるため、生物餌料が主流となって
おり、一般的な人工餌料が開発されていない。In addition, artificial diets have been developed for freshwater fish such as salmon and trout, while for hatchery larvae to grow into juveniles, for marine fish, the development of digestive organs after hatching is considered to be freshwater fish. Since it is different, biological feed has become mainstream, and general artificial feed has not been developed.
人工孵化した海産魚類の餌料系列は、以下の如くであ
る。The dietary line of artificially hatched marine fish is as follows.
上記のワムシ、アルテミア等の餌料生物は、クロレ
ラ、酵母又は微生物フロック等により増殖培養され、仔
稚魚等に与えられている。 The above-mentioned feed organisms such as rotifer and artemia are proliferated and cultivated by chlorella, yeast, microbial floc and the like, and fed to larvae and the like.
人工種苗生産時に、孵化仔魚がその成長過程において
大量に斃死する期間、いわゆるクリティカルピリオド
（critical period）が存在する事が良く知られてい
る。この期間をいかに無事に早く通過させるかが、人工
種苗生産時の一つの問題点となっている。It is well known that during the production of artificial seedlings, there is a so-called critical period during which the hatched larvae die in large amounts during the growth process. How to pass this period safely and quickly has become one of the problems in the production of artificial seedlings.
成魚に成長ホルモンを投与して成長を早める如く、仔
稚魚に成長ホルモンを投与してその成長を早める方法
は、仔稚魚期を短時間で通過させる事ができるため、上
記斃死の問題点の解決策の一つと成り得る。しかし、成
魚と異なり、仔稚魚期においては生物餌料しか摂餌せ
ず、仔稚魚への成長ホルモンの有効な投与法が無い事か
ら、上記方法の実施は困難であった。例えば、成長ホル
モンの投与法としては、成長ホルモン様物質を、餌料に
混入して投与する方法、胃に直接注入する方法及びカプ
セル化して胃に直接投与する方法等が考えられるが、仔
稚魚においては、いづれの投与法も実質上不可能である
か非効率的である。The method of administering growth hormone to larvae and accelerating their growth in the same way as administering growth hormone to adult fish to accelerate its growth is because the larval and juvenile stages can be passed in a short time. It can be one of the measures. However, unlike the adult fish, it is difficult to carry out the above-mentioned method because it feeds only the biological feed during the larval stage and there is no effective administration method of the growth hormone to the larval stage. For example, as a method of administering growth hormone, a method of mixing a growth hormone-like substance with a feed and administering it, a method of directly injecting it into the stomach, a method of encapsulating it directly into the stomach, etc. Is virtually impossible or inefficient with either method of administration.
従って、本発明の目的は、仔稚幼魚に魚類の成長ホル
モンを容易且つ効率的に取込ませることができる仔稚幼
魚の飼育方法を提供することにある。Therefore, an object of the present invention is to provide a method for rearing juvenile and juvenile fish that allows the juvenile and juvenile fish to easily and efficiently take up fish growth hormone.
本発明者らは、種々検討した結果、魚類の成長ホルモ
ン様物質を、餌料に混入して直接的に仔稚幼魚に経口投
与するのではなく、一度餌料生物に取り込ませ、この餌
料生物をキャリヤーとして仔稚幼魚に補食させることに
よって投与する方法、即ち生物の食物連鎖を利用する方
法により、前記目的が達成されることを知見した。As a result of various studies, the inventors of the present invention did not directly orally administer the fish growth hormone-like substance into the feed and directly orally administer it to the larvae and juveniles. As a result, it has been found that the above-mentioned object can be achieved by a method of administering by feeding to a juvenile and juvenile fish, that is, a method of utilizing the food chain of an organism.
本発明は、上記知見に基づきなされたもので、魚類の
成長ホルモン様物質の産生能を有する微生物で、餌料生
物を培養することにより、又は、魚類の成長ホルモン様
物質の産生能を有する微生物から抽出した魚類の成長ホ
ルモン様物質をマイクロカプセル化したもの若しくは魚
類から抽出した魚類の成長ホルモン様物質をマイクロカ
プセル化したものを、餌料生物に投与することにより、
魚類の成長ホルモン様物質を餌料生物に取込ませ、然る
後、この餌料生物を仔稚幼魚に投与することを特徴とす
る仔稚幼魚の飼育方法を提供するものである。The present invention has been made based on the above findings, by a microorganism having the ability to produce a growth hormone-like substance in fish, by culturing a feed organism, or from a microorganism having the ability to produce a growth hormone-like substance in fish. By microencapsulating the extracted fish growth hormone-like substance or microencapsulating the fish growth hormone-like substance extracted from fish, by administering to a feeding organism,
The present invention provides a method for raising juvenile and juvenile fish, which comprises incorporating a growth hormone-like substance of fish into a feeding organism, and then administering the feeding organism to the juvenile and juvenile fish.
以下、本発明の仔稚幼魚の飼育方法について詳述す
る。Hereinafter, the method for raising juvenile and juvenile fish of the present invention will be described in detail.
本発明で用いられる魚類の成長ホルモン様物質として
は、マグロ、タイ等の脳下垂体から抽出したもの、魚類
の成長ホルモン様物質の産生能を有する酵母、大腸菌、
枯草菌等の微生物本体及び該微生物から抽出したもの等
が挙げられる。The fish growth hormone-like substance used in the present invention, tuna, those extracted from pituitary gland such as Thailand, yeast having the ability to produce fish growth hormone-like substance, E. coli,
Examples include microbial bodies such as Bacillus subtilis and those extracted from the microorganisms.
また、上記成長ホルモン様物質を取込ませる餌料生物
としては、ワムシ、アルテミア、チグリオプス等、仔稚
幼魚の餌料生物として通常用いられているものを用いる
ことができる。As the feed organism for incorporating the growth hormone-like substance, those commonly used as feed organisms for larval and juvenile fish such as rotifer, artemia, and tigliops can be used.
而して、本発明を実施するには、先ず、上記成長ホル
モン様物質を上記餌料生物に取込ませる。この方法とし
ては、下記〜の方法が挙げられる。Therefore, in order to carry out the present invention, first, the growth hormone-like substance is incorporated into the feeding organism. Examples of this method include the following methods.
魚類の成長ホルモン様物質の産生能を有する酵母、大
腸菌、枯草菌等の微生物で、餌料生物を培養することに
より、魚類の成長ホルモン様物質を餌料生物に取込ませ
る方法。A method for incorporating a fish growth hormone-like substance into the feed organism by culturing the feed organism with a microorganism such as yeast, Escherichia coli, or Bacillus subtilis capable of producing the fish growth hormone-like substance.
魚類の成長ホルモン様物質の産生能を有する酵母、大
腸菌、枯草菌等の微生物から抽出した魚類の成長ホルモ
ン様物質をマイクロカプセル化したものを、餌料生物に
投与することにより、魚類の成長ホルモン様物質を餌料
生物に取込ませる方法。By microencapsulating a fish growth hormone-like substance extracted from a microorganism capable of producing a fish growth hormone-like substance, such as yeast, Escherichia coli, or Bacillus subtilis, into a feeding organism, A method of incorporating a substance into a feeding organism.
魚類から抽出した魚類の成長ホルモン様物質をマイク
ロカプセル化したものを、餌料生物に投与することによ
り、魚類の成長ホルモン様物質を餌料生物に取込ませる
方法。A method of incorporating a growth hormone-like substance of a fish into a feed organism by administering to the feed organism a microencapsulated substance of the growth hormone-like substance extracted from a fish.
上記〜の方法について更に説明すると、上記の
方法における餌料生物の培養は、魚類の成長ホルモン様
物質の産生能を有する微生物を餌料生物の餌料として使
用し、通常の餌料生物の培養法に従って実施すれば良
い。To further explain the above methods, the culture of the feed organism in the above method is carried out according to a usual culture method for the feed organism, using a microorganism having the ability to produce a growth hormone-like substance of fish as the feed for the feed organism. Good.
また、上記及びの方法で用いられる微生物は、遺
伝子組み換え技術を利用して製造することができる。Further, the microorganism used in the above and the above methods can be produced by using a gene recombination technique.
また、上記及びの方法における成長ホルモン様物
質のマイクロカプセル化は、常法に従って行えば良い。
マイクロカプセル化物の大きさは、径１〜50μｍが好ま
しいが、餌料生物の大きさにより適宜変更し得る。ま
た、マイクロカプセル化物の餌料生物への投与は、クロ
レラ等の餌料で培養している餌料生物に、餌料生物の仔
稚幼魚への投与前に集中的に行えば良い。Microencapsulation of the growth hormone-like substance in the above and the above methods may be performed according to a conventional method.
The size of the microencapsulated product is preferably 1 to 50 μm in diameter, but can be appropriately changed depending on the size of the feed organism. Further, the administration of the microencapsulated product to the feeding organism may be intensively performed on the feeding organism cultured in the feeding such as Chlorella before the administration of the feeding organism to the larvae of the fry.
然る後、上述の方法により成長ホルモン様物質を取込
ませた餌料生物を仔稚幼魚に投与する。この投与は、通
常の餌料生物の仔稚幼魚への投与と同様の方法により行
えば良い。Thereafter, the feeding organism in which the growth hormone-like substance is incorporated by the above-mentioned method is administered to the larvae and juveniles. This administration may be carried out in the same manner as in the administration of normal feeding organisms to juveniles and juveniles.
投与された餌料生物は仔稚幼魚に補食され、その結
果、餌料生物に取込まれた魚類の成長ホルモン様物質が
仔稚幼魚の有効に作用しその効果が発揮される。The administered feeding organism is supplemented by the young and juvenile fish, and as a result, the growth hormone-like substance of the fish taken up by the feeding organism acts effectively on the young and juvenile fish to exert its effect.
本発明の方法による飼育の対象となる魚類としては、
特に制限されるものではなく、例えば、マグロ、タイ、
スズキ、ヒラメ、ハマチ、フグ、シマアジ、サケ、マス
等が挙げられる。The fish to be raised by the method of the present invention include:
It is not particularly limited, for example, tuna, Thailand,
Examples include sea bass, flounder, yellowtail, blowfish, striped horse mackerel, salmon, and trout.
以下に実施例を挙げ、本発明を更に詳しく説明する。 Hereinafter, the present invention will be described in more detail with reference to Examples.
実施例１ 孵化後５日のマダイ仔魚（平均体長3.4mm）一群100匹
を40日間飼育した。Example 1 100 groups of red sea bream larvae (average body length 3.4 mm) 5 days after hatching were bred for 40 days.
試験区においては、飼育期間中、遺伝子組み換え技術
により製造したマグロ成長ホルモン産生能を有する酵母
で培養したワムシを１週間当たり２回の割合で投与して
飽食させ、その日以外はクロレラで培養したワムシを投
与して飽食させた。対照区においては、クロレラで培養
したワムシのみで飼育した。試験区及び対照区につい
て、飼育前及び飼育後の平均体長並びに成長率を下記第
１表に示す。In the test area, during the rearing period, rotifers cultured with a yeast having the ability to produce tuna growth hormone produced by gene recombination technology were administered twice a week to allow them to saturate, and other days, rotifers were cultured with chlorella. Was administered to cause satiety. In the control group, only rotifers cultured in Chlorella were bred. Table 1 below shows average body lengths and growth rates before and after breeding for the test plots and the control plots.
実施例２ 孵化後５日のクロダイ仔魚一群50匹を30日間、実施例
１と同様に飼育した。その結果を下記第２表に示す。 Example 2 A group of 50 black larvae larvae 5 days after hatching was bred for 30 days in the same manner as in Example 1. The results are shown in Table 2 below.
実施例３ 孵化後５日のヒラメ仔魚一群50匹を40日間飼育した。 Example 3 A group of 50 flounder larvae 5 days after hatching was bred for 40 days.
試験区においては、飼育期間中、遺伝子組み換え技術
により製造したマグロ成長ホルモン産生能を有する大腸
菌から抽出した成長ホルモン（50％純度）を、ミルクカ
ゼインから作成した直径15〜20μｍのマイクロカプセル
を用いてマイクロカプセル化したものを、仔魚投与前の
ワムシに充分に取り込ませ、このワムシを１週間当たり
３回の割合で投与して飽食させた。その他は、実施例１
と同様に飼育した。その結果を下記第３表に示す。In the test area, during the breeding period, growth hormone (50% purity) extracted from Escherichia coli capable of producing tuna growth hormone produced by gene recombination technology was used, and microcapsules made from milk casein with a diameter of 15 to 20 μm were used. The microencapsulated product was sufficiently incorporated into the rotifer before the administration of the larva, and the rotifer was administered at a rate of 3 times per week to saturate. Otherwise, Example 1
Breed in the same manner as. The results are shown in Table 3 below.
〔発明の効果〕 本発明の仔稚幼魚の飼育方法によれば、仔稚幼魚に魚
類の成長ホルモンを容易且つ効率的に取込ませることが
できるため、仔稚幼魚期を短時間で通過させる事がで
き、斃死率を減少させると共に沖出し時期や放流時期を
早める事が可能となった。 [Effects of the Invention] According to the method for rearing juvenile and juvenile fish of the present invention, it is possible to allow the juvenile and juvenile fish to take up growth hormone of fish easily and efficiently. It has become possible to reduce the death rate and accelerate the time of offshore release and release.
Claims (1)
る微生物で、餌料生物を培養することにより、又は、魚
類の成長ホルモン様物質の産生能を有する微生物から抽
出した魚類の成長ホルモン様物質をマイクロカプセル化
したもの若しくは魚類から抽出した魚類の成長ホルモン
様物質をマイクロカプセル化したものを、餌料生物に投
与することにより、魚類の成長ホルモン様物質を餌料生
物に取込ませ、然る後、この餌料生物を仔稚幼魚に投与
することを特徴とする仔稚幼魚の飼育方法。1. A fish growth hormone-like substance extracted by culturing a feed organism with a microorganism capable of producing a fish growth hormone-like substance or extracted from a microorganism capable of producing a fish growth hormone-like substance. The microencapsulated product or microencapsulated fish growth hormone-like substance extracted from fish is administered to the feeding organism to allow the fish growth hormone-like substance to be incorporated into the feeding organism. , A method for raising juveniles and juveniles, which comprises administering this feeding organism to juveniles and juveniles.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62170505A JP2558715B2 (en) | 1987-07-08 | 1987-07-08 | How to rear young larvae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62170505A JP2558715B2 (en) | 1987-07-08 | 1987-07-08 | How to rear young larvae |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6413928A JPS6413928A (en) | 1989-01-18 |
| JP2558715B2 true JP2558715B2 (en) | 1996-11-27 |
Family
ID=15906199
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62170505A Expired - Lifetime JP2558715B2 (en) | 1987-07-08 | 1987-07-08 | How to rear young larvae |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2558715B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372252A (en) * | 2017-08-29 | 2017-11-24 | 大连海洋大学 | Extra large huge legendary turtle shrimp offspring seed cultivation method |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6447355A (en) * | 1987-08-18 | 1989-02-21 | Taiyo Fishery Co Ltd | Raising of young fish |
| KR20050114004A (en) * | 2004-05-31 | 2005-12-05 | (주)진투프로테인 | The growth and survival enhancement method of shrimp larvae using recombinant bovine growth hormone |
| CN113854205A (en) * | 2021-11-09 | 2021-12-31 | 宁德市鼎诚水产有限公司 | Large-scale fingerling ecological polyculture method for epinephelus akaara |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5944020A (en) * | 1982-09-06 | 1984-03-12 | Nippon Telegr & Teleph Corp <Ntt> | Directional phase shifter |
| JPH0730656B2 (en) * | 1984-07-17 | 1995-04-10 | 株式会社ツ−デン | Automatic door controller |
| JPH062065B2 (en) * | 1985-07-22 | 1994-01-12 | 協和醗酵工業株式会社 | Fish growth hormone gene and polypeptide encoding the gene |
-
1987
- 1987-07-08 JP JP62170505A patent/JP2558715B2/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372252A (en) * | 2017-08-29 | 2017-11-24 | 大连海洋大学 | Extra large huge legendary turtle shrimp offspring seed cultivation method |
| CN107372252B (en) * | 2017-08-29 | 2020-08-04 | 大连海洋大学 | Method for cultivating young crayfish |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6413928A (en) | 1989-01-18 |
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