JPH10177022A - Method for manufacturing blood sedimentation tube and synthetic resin body used for it - Google Patents

Abstract

PROBLEM TO BE SOLVED: To improve a method for manufacturing a conventional sedimentation tube and a synthetic resin body used for it. SOLUTION: In the sedimentation tube 1, a porous synthetic resin body 5 with a property for passing a gas but preventing a liquid from passing is wrested into a specific position from either opening of a synthetic resin tube 2, which has a uniform inner diameter but has both edges being open and either opening becomes a liquid flow-in port. In this case, the synthetic resin pipe 2 has a water repellency and the porous synthetic resin body 5 is composed of a water-repellent porous body, and at the same time a macromolecule with a high water absorption property in xero gel shape is contained by 10.0-20.0wt.% for the total weight in the water-repellent porous body. The synthetic resin body is manufactured by half-melting and forming the particle body of a thermoplastic resin as a porous body and forming the porous body in a specific shape and dipping it into a silicone oil, and then drying it.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to blood sedimentation tubes.
Specifically, a porous synthetic resin body having a property of allowing gas to pass therethrough but not allowing liquid to pass therethrough is inserted into a predetermined position from one of the openings of a conventional synthetic resin tube having a uniform inner diameter and having both ends open. The present invention relates to an improvement in a blood-sedimentation tube which is attached to one of the openings and the other opening is used as a liquid inlet.

[0002]

2. Description of the Related Art A conventional blood sedimentation tube is made of a glass tube having a length of 30 cm and an inner diameter of 3 mm, both ends being open. The glass tube is graduated 1 mm over 20 cm from one end opening. An inspection using such a conventional blood sedimentation tube is performed as follows. First, a blood sedimentation anticoagulant, for example, 3.8% sodium citrate, is added to the blood, and then the blood is sucked by a person to reduce the pressure in the blood sedimentation tube and put the blood to the zero point (base point) of the blood sedimentation tube.
Alternatively, blood was injected into the blood sediment tube up to point 0 using a syringe or the like. Next, after the blood sedimentation tube was allowed to stand vertically, the sedimented interface of red blood cells was measured every hour. Further, after completion of the blood sedimentation test, the used blood sedimentation tube was washed, sterilized, dried, etc. to regenerate the blood sedimentation tube. However, in order to perform a blood sedimentation test using such a conventional blood sedimentation tube, it is necessary for a person to suck blood by mouth or use a syringe to put blood to the zero point of the blood sedimentation tube, and furthermore, this operation is simple. Although it is skilled, it takes time for manual work and it is difficult to process many samples in a short time. In addition, in the above-mentioned work process or the work process such as washing by recycling, sterilization, and drying, a medical worker may have a blood-derived disease, such as viral hepatitis,
There is a possibility that AIDS or the like will be completed in the hospital, and the work involved in recycling will also be troublesome. In addition, if the above-mentioned blood sedimentation tube is used for a long period of time, fats, proteins, and the like adhere to the wall surface, so that the washing is likely to be incomplete, and the obtained data may be incomplete.

In order to solve such disadvantages, Japanese Patent Application No. 63-13735, filed by the inventor of the present invention, discloses a method.
There is No. 3. In this blood sedimentation tube, a multi-resistive synthetic resin body (containing a superabsorbent polymer) that allows gas to pass but does not allow liquid to pass through is inserted into a synthetic resin tube having a uniform inner diameter and open at both ends. At the time of use or in the collected blood, put the liquid inlet side of the sedimentation tube into the blood and reduce the pressure in the sedimentation tube from the other end of the sedimentation tube, thereby simultaneously introducing a large number of blood to the insertion position of the porous synthetic resin body. Is what you can do.

[0004] Furthermore, the conventional blood sedimentation tube was improved, and Japanese Patent Publication No. 7-82012 (a method for producing a blood sedimentation tube and a water repellent porous synthetic resin body used therein) was developed and disclosed by the present inventor. I have. However, in a blood sedimentation tube in which a porous synthetic resin body that allows gas to pass through but not liquid through the synthetic resin tube according to this improved invention is used when the porous synthetic resin body is used due to water infiltration or when the porous synthetic resin body is used. If even a small amount of water drops or blood is inadvertently applied, the air permeability will be instantaneously lost, and it will be impossible to use the blood-sedimentation tube.
In addition, since the porous synthetic resin body is water-immersed, blood cannot be stopped unless the pore diameter of the porous synthetic resin body is small and its length is long. As a result, it is necessary to increase the suction pressure in the blood sedimentation tube, and when the liquid comes into contact with a superabsorbent polymer such as corn starch in the xerogel state, the corn starch in the xerogel state on the surface of the porous synthetic resin body becomes a normal gel. In addition, this gel flows out into the blood sedimentation tube and affects sedimentation of red blood cells. Therefore, the present invention does not prevent the use of liquids such as water and blood by inadvertently losing air permeability and making it unusable.In addition, the suction pressure is increased and the superabsorbent polymer flows out into the blood. It is an improved blood-sedimentation tube.

[0005]

There is a problem to be solved by the present invention in that such a conventional blood sedimentation tube has been improved.

[0006]

SUMMARY OF THE INVENTION The present invention has been developed in order to solve the above-mentioned problems, and has a uniform inner diameter and is open at both ends. At a predetermined position from the opening, a porous synthetic resin body having a property of passing gas but not passing liquid is inserted, and in the blood sedimentation tube having one of the other openings as a liquid inlet, the synthetic resin tube is repellent. And the porous synthetic resin body is composed of a water-repellent porous body, and the water-repellent porous body contains a xerogel-like superabsorbent polymer in an amount of 10.0 to 20.0% by weight based on the total weight thereof. It is intended to provide a blood sedimentation tube characterized by being contained, and a porous synthetic resin body is inserted into the synthetic resin tube and several drops of water are dropped through a dropper, and after a few minutes, a vacuum pump is operated. Via negative pressure 500mm
Hg, the pressure difference was measured, the length of the porous synthetic resin body was formed to be 1.0 mm to 3.0 mm, the porous synthetic resin body was inserted into the synthetic resin tube, and the liquid flow was measured. The inlet is inserted into the blood, and is then passed through a vacuum pump.
The purpose of the present invention is to provide a blood sedimentation tube for observing the blood flow state by suctioning at 0 mmHg for several minutes.

Further, a blood sedimentation tube characterized in that a thermoplastic resin powder or granule is semi-melted and formed into a porous body, and the porous body is formed into a predetermined shape, immersed in silicone oil and then dried. The synthetic resin body is a method for producing a synthetic resin body, wherein the synthetic resin body has a colored circular shape having a length of 3 mm, and has a property of passing gas but not liquid. Wherein the porous synthetic resin body is used for a blood-sedimentation tube made of a thermoplastic resin having water repellency such as polyethylene, polypropylene, ethylene, vinyl acetate copolymer, and polystyrene.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION The blood sedimentation tube of the present invention has a uniform inner diameter and allows gas to pass but not liquid to a predetermined position from one of the openings of a synthetic resin tube having both ends open. In a blood sedimentation tube in which a porous synthetic resin body having characteristics is inserted and one of the other openings is a liquid inlet, the synthetic resin tube is made to be water-repellent and the porous synthetic resin body is made of a water-repellent porous body. And a xerogel-like superabsorbent polymer is added to the repellent porous body based on the total weight thereof.
The porous synthetic resin body is inserted into the synthetic resin tube, and a few drops of water are dropped through a dropper. After a minute, a negative pressure of 500 mmHg is suctioned through a vacuum pump to measure a differential pressure, and the length of the porous synthetic resin body is formed to be 1.0 mm to 3.0 mm. A blood sedimentation tube that is inserted into the tube, inserts the liquid inlet into the blood, and is further suctioned at 500 mmHg for several minutes through a vacuum pump to observe the blood flow state. Can be provided.
That is, according to the blood sedimentation tube of the present invention, even if a small amount of water or blood is inadvertently brought into contact with the porous synthetic resin body in the blood sedimentation tube, both the synthetic resin tube and the porous synthetic resin body in the blood sedimentation tube are repellent. Therefore, not only does water and blood repel water and blood, but the water and blood hardly reach the superabsorbent polymer, and the superabsorbent polymer does not act. In addition, since the porous synthetic resin body is a water-repellent porous body, blood can be stopped even if the pore size is large and short in the longitudinal direction as compared with the water-penetrating one, and blood is sucked even if the suction pressure is low. After the water or blood comes into contact with the pore-absorbing pore molecules, the water and blood are not only immediately sealed but also not immersed, so that the pore-absorbing pore molecules hardly flow to the blood side. Further, since the porous synthetic resin body is colored, the starting point of the scale can be seen at a glance.

Further, the production method of the present invention is characterized in that a thermoplastic resin powder or granule is semi-melted and formed into a porous body, and the porous body is formed into a predetermined shape, immersed in silicone oil and then dried. A method for producing a synthetic resin body used for a blood-sedimentation tube, wherein the synthetic resin body is colored 3 m in length.
m, made of a porous synthetic resin having the property of allowing gas to pass but not liquid, and further comprising a porous synthetic resin such as polyethylene, polypropylene, ethylene / vinyl acetate copolymer, polystyrene, etc. This is a method for producing a synthetic resin body used for blood-sedimentation tubes made of a thermoplastic resin having water repellency, so that the porous body may contain water-absorbing pore molecules, so that the resin itself does not have water repellency. It is also possible to easily produce a rupture-resistant porous synthetic resin body.

[0010]

Embodiments of the present invention will be described below with reference to the accompanying drawings. The drawing is a perspective view of the blood sedimentation tube of the present invention. In the drawing, reference numeral 1 denotes a blood sedimentation tube, and the blood sedimentation tube 1 is made of a synthetic resin tube 2 having water repellency, such as polyethylene, polystyrene, or polypropylene. The synthetic resin tube 2 may be made of a non-water repellent material, in which case it is necessary to perform a water repellent treatment. The synthetic resin tube 2 has a uniform inner diameter and is open at both ends. One end is a liquid inlet 3 through which a liquid such as blood flows. The other end of the synthetic resin tube 2 is formed in a tapered shape, and the tapered opening 4 is provided so that the porous synthetic resin body 5 can be easily inserted into a predetermined position in the synthetic resin tube 2.

The porous synthetic resin body 5 has a property of allowing gas to pass therethrough but not liquid, and is colored. The coloring is such that the positions of the porous synthetic resin bodies 5 which are usually used in three synthetic colors and which are inserted into the synthetic resin tube 2 can be easily confirmed. The porous synthetic resin body 5 has a cylindrical shape and a length of 3 mm. The synthetic resin pipe 2 is graduated from the liquid inlet 3 side at intervals of 1 mm at 20 cm, that is, at a zero point (base point). It is inserted into the synthetic resin tube 2 so that its one end face 5a is located. The porous synthetic resin body 5 has a length of 3
mm, it does not move in the synthetic resin tube 2 even if there is a differential pressure of ² kg / cm ² . Note that the shape of the porous synthetic resin body 5 is not limited to a columnar shape, and may be a spherical shape or another shape. Further, the porous synthetic resin body 5 is a porous body obtained by semi-solid molding a powder of a thermoplastic resin whose resin itself has water repellency, such as polyethylene, polypropylene, ethylene, vinyl acetate copolymer, and polystyrene. As will be described in more detail later, for example, the water-repellent water-repellent porous body contains 10.0 to 20.0% by weight of a superabsorbent polymer (xerogel) based on the total weight of the porous body. Some have been obtained. And this porous synthetic resin body 5
Although the resin itself has water repellency, it retains water repellency even when it is semi-melted, but since it is porous, it absorbs the liquid well due to the capillary phenomenon and consequently exhibits water immersion. Here, the semi-solid formation means that the filling layer is pressurized for the purpose of adjusting the porosity by filling the above-mentioned thermoplastic resin powder into a mold having a predetermined shape, and further pressing the powder-particle filling layer. Is a method of uniformly heating and cooling when the contact surface of the particles is fused in a semi-molten state to form a three-dimensional space (pores) in which voids between the particles are completely continuous. The polymer refers to a polymer compound having a water absorbing and sealing ability, such as corn starch, wheat starch, rice starch, rice starch, gelatin, agar, gum arabic, etc. Means Accordingly, the porous synthetic resin body 5 is obtained by subjecting the above-mentioned water-repellent thermoplastic resin granules to semi-melt molding, and then subjecting the repellent water treatment to a repellent porous body, whereby the superabsorbent polymer in a xerogel state is added. Since it is closely adhered, it allows gas such as air to pass through, but the liquid such as blood adheres the superabsorbent polymer in the xerogel state to the porous body. When the liquid comes into contact with the porous synthetic resin body 5, the superabsorbent polymer in the xerogel state instantly absorbs the liquid, and the superabsorbent polymer swells and does not pass through the liquid.

Next, a method of using the blood sedimentary tube having the above-described configuration will be described. First, the blood sedimentation tube 1 of the present invention is prepared, and the liquid inlet 3 of the blood sedimentation tube 1 is put into a test tube or the like containing blood that has been prevented from coagulation by sodium citrate. At this time, even if water droplets or blood is inadvertently dropped on the blood sedimentation tube 1, the synthetic resin tube 2 and the porous synthetic resin body 5 are both water repellent, so that the water droplets and blood are xerogel-like superabsorbent polymers. Do not reach. Therefore, the blood sedimentation tube 1 can be used as long as a small amount of water droplets or blood is generated.

Next, a tapered opening 4 is formed by a vacuum pump or the like.
When the inside of the synthetic resin pipe 2 is decompressed from the side, the porous synthetic resin body 5
Can withstand a pressure difference of ² kg / cm ² , so that blood can enter the synthetic resin tube 2 from the liquid inlet 3 without moving inside the synthetic resin tube 2. When this blood reaches the point 0 on the scale, that is, one end face 5a of the porous synthetic resin body 5, the superabsorbent polymer in a xerogel state closely adhered to the porous synthetic resin body 5 instantaneously absorbs water in the blood. The blood swells and becomes a sealed state, and the blood stops at zero point. At this time, since the porous synthetic resin body 5 has water repellency, even if the pore diameter of the porous body is large, for example, 30 μm, it is difficult to pass blood but easy to ventilate, so that blood is sucked at a low suction pressure. can do. Thereafter, when the blood reaches the porous synthetic resin body 5, the superabsorbent polymer immediately swells and becomes a sealed state as described above. The chance of contact with the conductive polymer is relatively small and hardly flows out to the blood side. As a result, the gel does not affect the sedimentation state of red blood cells. The operation of putting blood into the blood sedimentation tube 1 up to the zero point can be performed easily and instantly. Thereafter, the blood sedimentation tube 1 is set vertically, and the sedimentation state of red blood cells may be measured at predetermined time intervals. After the measurement, the used blood sedimentation tube 1 is discarded.

Next, data of a comparison experiment between the blood sedimentation tube of the present invention and a conventional blood sedimentation tube are shown. (Experimental Example 1) As shown in FIG. 2, two drops of water (one drop of about 0.05 ml) were placed in a blood sedimentation tube in which a porous synthetic oil / fat body was inserted into a synthetic resin tube 2 as shown in FIG. After 5 minutes, the differential pressure is measured by suctioning with a vacuum pump at a negative pressure of 500 mmHg. Further, for comparison, the pressure difference when no water is applied is measured by the same method as described above.

(Experimental Example 2) The same measurement as in Experimental Example 1 is performed using a blood sedimentation tube in which a porous synthetic resin body subjected to water repellent treatment (silicon treatment) is inserted into a synthetic resin tube 2.

Comparative Example 1 The same measurement as in Experimental Example 1 is performed using a blood sedimentation tube in which a water-permeable porous synthetic resin body is inserted into a synthetic resin tube 2.

The measurement results are as shown in Table 1.

[0018]

[Table 1] From Table 1, Experimental Examples 1 and 2 show no difference in pressure when water is applied and no pressure difference when water is not applied. In Comparative Example 1, however, the mist enters the porous synthetic resin and gels. Can be predicted to be crushed. In addition, although the pressure difference in the state where water is applied is significantly different between the experimental examples 1 and 2 and the comparative example 1, since the comparative example 1 is infiltrating and hardly stops blood, a porous body having a small pore diameter is used. This is because it is used for a synthetic resin body.

(Experimental Examples 3 to 7) As shown in FIGS. 3 and 4, the liquid inlet of the blood sedimentation tube of Experimental Example 1 having a length of 1 mm to 3.0 mm was put into blood. Then, blood is sucked at 500 mmHg for 5 minutes through a vacuum pump, and the state of blood flow is visually observed.

(Comparative Examples 2 to 6) With respect to the blood sedimentation tube of Comparative Example 1 in which the length of the porous synthetic resin body was 1 mm to 3.0 mm,
The same measurement as in Experimental Example 3 is performed.

The measurement results are as shown in Table 2.

[0022]

[Table 2] From Table 2, Comparative Examples 2, 3, and 4 were compared with Examples 3, 4, and 5.
Indicates that the blood is clearly passing and that the blood is hard to stop, and by making the water turbulent, the blood is easily stopped. This also indicates that since Examples 3, 4, and 5 are not water-immersed, the gelled superabsorbent polymer due to moisture in the blood does not easily flow into the blood.

[0023]

According to the blood sedimentation tube of the present invention, even if a small amount of water or blood is inadvertently dropped on the blood sedimentation tube, both the synthetic resin tube and the porous synthetic resin body in the blood sedimentation tube are repellent. In addition, it repels water and blood, making it difficult for water and blood to reach the superabsorbent polymer, and the superabsorbent polymer does not act. In addition, since the porous synthetic resin body is a water-repellent porous body, it can stop blood even if it has a large pore diameter and is short in the longitudinal direction as compared with a water-impregnated one, and sucks blood even if the suction pressure is low. Water and blood are sealed immediately after the water and blood come into contact with the superabsorbent polymer. Furthermore, since it is not water-immersed, it becomes difficult for the superabsorbent polymer to flow to the blood side.

Accordingly, even if liquid such as water or blood is inadvertently applied to the blood sediment tube, if the amount becomes large, the blood sediment tube can be used as it is, so that the handling is easy. In addition, since a water repellent porous material having a large pore size can be used, blood can be sucked even at a low suction pressure, and the high-absorbent polymer in a gel state hardly flows into the blood, affecting the sedimentation state of red blood cells. Has the effect of not exerting any effect. In addition, when the porous synthetic resin body is colored, in addition to the above-described effects, since the base point of the scale is changed at a glance, handling of the blood sedimentation tube is further facilitated.

Furthermore, since one end surface of the porous synthetic resin body is positioned at a base position by attaching a scale from the fluid inlet side, blood such as blood can reach the base point of the scale by suction from the opening side. Immediately flows in and no more flows in due to the porous synthetic resin body. Therefore, it is not necessary to adjust the liquid such as blood to the base point of the scale, so that the reference work of the blood sedimentation test becomes easy.

[Brief description of the drawings]

FIG. 1 is a perspective view showing a blood sedimentation tube of the present invention.

FIG. 2 is a schematic view showing an experimental example of the present invention.

FIG. 3 is a schematic view showing an experimental example of the present invention.

FIG. 4 is a schematic view showing an experimental example of the present invention.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 Blood sedimentation tube 2 Synthetic resin tube 3 Liquid inflow port 4 Tapered opening (one opening) 5 Porous synthetic resin body 5a One end surface

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification code FI C08J 9/36 C08J 9/36

Claims (5)

[Claims] 1. A porous synthetic resin body having a characteristic of allowing gas to pass therethrough but not allowing liquid to pass therethrough at a predetermined position from one of openings of a synthetic resin pipe having a uniform inner diameter and open at both ends. In the blood-sedimentation tube which is inserted and one of the other openings is a liquid inlet, the synthetic resin tube is made to be water-repellent and the porous synthetic resin body is made of a water-repellent porous material. A blood sedimentation tube containing 10.0 to 20.0% by weight of a xerogel-like superabsorbent polymer based on the total weight of the tube. 2. A porous synthetic resin body is inserted into the synthetic resin tube, a few drops of water are dropped through a dropper, and a few minutes later, a negative pressure of 500 mmHg is suctioned through a vacuum pump to reduce a differential pressure. The blood sedimentation tube according to claim 1, which is measured. 3. The length of the porous synthetic resin body is 1.0 m.
m to 3.0 mm, the porous synthetic resin body is inserted into a synthetic resin tube, and a liquid inlet is inserted into blood.
3. The blood sedimentation tube according to claim 1, wherein the blood is further sucked at 500 mmHg for several minutes via a vacuum pump to observe the state of blood flow. 4. A blood sedimentation tube characterized in that a thermoplastic resin powder is semi-melted and formed into a porous body, and the porous body is formed into a predetermined shape, immersed in silicone oil, and then dried. Production method of synthetic resin body used for 5. The colored synthetic resin body is 3 mm in length.
Made of a porous synthetic resin having a characteristic of passing a gas but not a liquid, wherein the porous synthetic resin further comprises polyethylene, polypropylene, ethylene, a vinyl acetate copolymer, polystyrene, etc. The method for producing a synthetic resin body used for a blood sedimentation tube according to claim 4, comprising a thermoplastic resin having water repellency.