JPH10146184A - Device for filtering and discharging specimen - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide the disposable device enabling to separate the solid component and the solution component of a specimen from each other and discharge a necessary volume of the solution component. SOLUTION: This device for filtering and discharging of a specimen comprises a cylindrical main part 10 having a fine penetrated hole 11 at the tip, a filter member 20 disposed in the inner tip side of the main part 10, and a press cap 30 disposed at the base end of the main part 10. A specimen is charged on the filter member 20 in the main body 10, dissolved in a solvent such as water, and subsequently filtered with the filter member 20. A solution 41 in which bacteria contained in the specimen are floated is temporarily stored below the filter member 20 in the main part. When the press part 32 of the press cap 30 is pressed, only the solution containing the floated bacteria is discharged through the tip penetrated hole 11 of the main part.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to E. coli O.
When testing for the presence of bacteria such as -157 or the like, a test solution is prepared from a subject, and the required amount of the test solution is determined by using a test plate or a test stick (stic) coated with a test agent.
It relates to a tool for attaching to, for example, k).

[0002]

2. Description of the Related Art Conventional methods for testing bacteria include, for example, when testing for the presence of Escherichia coli O-157 in stool, a small amount of collected stool is placed in a tester, and this is dissolved in water. Injecting a drug called a buffer into it, separating the solid components of the subject, sucking only a small amount of the aqueous solution component in which bacteria swim, using a dropper, etc., and attaching it to a test stick etc. . In this case, it is extremely preferable that an extremely small amount of an aqueous solution component of about 0.07 ml is attached to the test plate, and if a larger amount of the test solution is applied, the test result will not appear clearly. .

[0003]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide a disposable and simple tool capable of separating a solid component and a solution component of a subject and discharging a required amount of the solution component. The purpose of the present invention is to provide a more sanitary tool by eliminating the trouble of taking out the test solution with a dropper or the like as in the past, thereby preventing the hands and clothes of the inspector from being contaminated with bacteria. It is.

[0004]

SUMMARY OF THE INVENTION In order to solve the above-mentioned problems, the present invention provides a main body 10 comprising a cylindrical body having a very thin through hole 11 at the front end, and a main body 10 provided on the inner front side of the main body 10. And a pressure means such as a pressing cap 30 provided at the base end of the main body 10. The subject is placed above the filtration member 20 in the main body 10, and a solvent such as water By dissolving the subject and passing through the filtering member 20, only the solution in which the bacteria in the subject swim is temporarily stored below the filtering member 20 in the main body, and pressurized by pressing the pressing cap 30 or the like. Only the solution in which the bacteria swim by the operation of the means can be discharged to the outside through the through hole 11 at the tip of the main body 10.

In the present invention, for example, a small amount of a patient's stool which is thought to have been infected with E. coli O-157 or the like as a subject is attached to the inner wall of the main body 10 above the filter member 20, and water is added thereto. , And the mixture is stirred with, for example, a stirring rod to dissolve the subject in water as a solvent. After a while, the solid component in the solution is filtered by the filter member 20, and only the solution in which bacteria swim is stored below the filter member 20. At this time, an extremely fine through-hole 11 is provided at the tip of the main body 10, but the solution does not leak from the through-hole 11 due to the viscosity or surface tension of the solution. After a certain period of time, when the solution containing bacteria accumulates inside the lower end of the main body 10, the main body 1
By attaching a pressing means such as a pressing cap 30 to the base end above the zero and pressing the pressing cap 30 with a finger or the like, a required amount of the test solution can be attached to the test stick or the like.

[0006] Extra-fine through-hole 1 provided at the tip of the main body
The diameter of 1 is equivalent to the diameter of the hole of the needle of the syringe,
It is about 1/3 mm to 1 mm. As described above, the filtration and
By the discharge tool, the hands and clothes etc. are not contaminated with bacteria, and in the required amount, for example, the above-mentioned test stick, etc.
A very small amount of the test solution of about 0.07 ml can be discharged and attached. The solvent for dissolving the analyte is not limited to water, and it is necessary to use other solvents other than water, for example, for blood tests. Further, in order to dissolve the subject in a solvent, the main body 10 can be shaken and stirred without using the above-described stirring rod.

[0007]

Embodiments of the present invention will be described below in detail with reference to the accompanying drawings. FIG. 1 shows a central longitudinal sectional view of a filtering / discharging tool according to the present invention, in which
And a filter member 20 provided on the inner tip side of the main body 10.
And a pressing cap 30 as pressing means attached to a base end above the main body 10. The main body 10 has a substantially cylindrical shape, and a lower end portion thereof is tapered, and an extremely fine through hole 11 is formed at the lower end portion. The inside diameter of the through hole 11 is substantially the same as the inside diameter of the syringe needle of about 3/1 mm to 1 mm. The length of the through hole 11 may be about 5 mm or more. The inner diameter of the upper part of the main body 10 is also about 1.0.
The thickness is about 1 to 5 cm, and the thickness can be determined as needed. In the present embodiment, the overall length of the main body 10 in the longitudinal direction is also approximately 4 to 8 cm. The material of the main body 10 is made of a translucent or transparent synthetic resin, and can be disposable after use.

As the filter member 20 disposed on the inner front end side of the main body 10, various types of filter paper, filter cloth and other materials can be used, but an appropriate mesh member is selected according to the type of the subject. do it. In this embodiment, a filter obtained by joining a filter paper on a synthetic resin net is used. The filtering member 20 is provided with a conical portion 12 inside the main body 10.
Is fixed to the upper end.

In the opening at the base end above the main body 10,
A pressing cap 30 as a pressing means is attached. The pressing cap 30 includes a synthetic resin cap section 31 fitted into the opening of the main body section 10 and a rubber pressing section 32 covering the cap section 31 from above. The through hole 33 is formed so that the pressure inside the main body 10 is increased by pressing the pressing portion 32 downward from above with a finger or the like. The pressing portion 32 may be made of synthetic resin instead of rubber.

The method of using the filtering / discharging tool is as follows. A small amount of the stool 40 collected from a subject, for example, a patient suspected of being infected with E. coli O-157
0 is attached to the upper wall surface of the filter member 20 inside. In this case, water is introduced as a solvent into the main body 10 and stirred with a stirring rod to dissolve the subject in the water. This causes the bacteria to swim in the solvent. The stir bar is
Disposable paper can be used, and the main body 10 can be shaken and stirred without using a stirring rod. Here, when adding a solvent and stirring, a certain coagulant,
And, in some cases, antibiotics (such as FOM) can be added. By allowing a little time in this state, the solid components in the solution are removed by the filter member 20, and only the solution 41 in which bacteria swim is stored below the filter member 20.

After leaving for a certain period of time, the solution 4
In order to attach 1 to an inspection stick or the like, the pressing cap 30 is fitted into the opening at the base end of the main body 10, and the pressing section 32 is pressed with a finger or the like to form a through hole at the lower end of the main body 10. 1
The required amount of solution 41 can be discharged through 1. In this tool, since the inner diameter of the through hole 11 formed at the lower end of the main body 10 is extremely small, the solution 41 to be inspected filtered by the filter member 20 is not affected by its own viscosity or surface tension. The inside of the main body 10 is pressurized only when the pressing portion 32 of the upper pressing cap 30 is pressed without leaking to the outside from the through hole 11, so that the solution 41 to be inspected is outside the main body 10. Is to be discharged. As described above, in the present invention, even if the required amount of the solution to be inspected is necessary, for example, a very small amount of the inspection solution of about 0, It can be attached.

Although the present invention has been described above, the embodiment can be modified as follows. First,
The material, shape, size, and the like of the main body 10 and the pressing means can be appropriately changed as needed.
However, in order to make this tool disposable, it is desirable that its material, shape, size, and the like be inexpensive. The main body portion 10 is formed of a cylindrical body, and any object may be used as long as the object can be put in the inside thereof and the object can be dissolved with a solvent.
Can be designed freely.

The configuration of the pressing cap 30 as the pressing means is completely free, and the whole may be formed from a rubber cap. Further, a clip or the like may be combined with the opening of the rubber cap so that the opening can be easily attached to the opening of the main body 10. A female screw is provided on the inner peripheral surface of the cap portion 31 of the pressing cap 30, and a male screw is provided on the outer peripheral portion of the upper base end of the main body portion 10.
The pressing cap 30 may be screwed to the main body 10. Furthermore, it goes without saying that the pressing means may be a plunger or a piston-type pressing body instead of the pressing cap.

The pressurizing means is not disposable, but only the pressurizing means such as the pressing cap 30 can be used repeatedly, and only the main body 10 provided with the filter member 20 can be disposable. . Immediately after the subject is put in the main body 10 and dissolved in water, the pressing cap 30 may be attached and placed. In that case, a small through hole is provided at an appropriate position above the main body 10 and an air inlet is provided so that the solution to be inspected is filtered well and can be dropped below the filtering member 20. Need to put. Then, when pressurizing the filtered test solution, the press cap 30 may be pressed while the through-hole is closed with a finger.

[0015]

In the present invention according to the above aspect, the solid component of the subject and the solution component containing the bacteria and the components of the bacteria are separated by one tool, and even if the amount is extremely small, It is possible to discharge a required amount of the solution to be inspected, thereby preventing bacterial contamination of the hands and clothes of the inspector. In addition, the tool according to the present invention,
It can be carried out as a disposable one, and by disposing after use, it is possible to eliminate the trouble of cleaning and sterilizing the tool and to perform more sanitary treatment. As described above, the present invention
This is a simple configuration that exhibits a great effect.

[Brief description of the drawings]

FIG. 1 is an overall central longitudinal sectional view of an embodiment of a tool according to the present invention.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 10 ... Body part, 11 ... Through-hole, 20 ... Filter member, 30 ...
Press cap

Claims (1)

[Claims] 1. A main body (10) comprising a cylindrical body having an extremely fine through-hole (11) at a front end, a filtering member (20) provided at an inner front end of the main body (10), and a main body. (10) is composed of pressing means such as a pressing cap (30) provided at the base end portion, and the subject is placed above the filtering member (20) in the main body (10), and is subjected to a solvent such as water. Only the solution in which the bacteria in the subject swim by temporarily dissolving the subject and passing through the filtering member (20) is temporarily stored below the filtering member (20) in the main body, and presses the pressing cap (30). A device for filtering and discharging a subject, wherein only a solution in which bacteria swim by actuation of a pressurizing means such as a filter can be discharged to the outside through a through hole (11) at the tip of a main body (10). .