JPH09294592A - Decomposition of sodium normal-alkylbenzenesulfonate - Google Patents

Abstract

PROBLEM TO BE SOLVED: To efficiently degrade sodium normal-alkylbenzenesulfonate, a surfactant for detergent, by culturing a microorganism in Bacillus capable of degrading sodium normal-alkylbenzenesulfonate in a medium containing the alkylbenzenesulfonate. SOLUTION: A microorganism in Bacillus capable of degrading sodium normal alkylbenzenesulfonate, for example, Bacillus sp. No. E61806(FERM P-15576) is inoculated in a culture medium containing sodium normal- alkylbenzenesulfonate and reduced type nicotinic amide adenine dinucleotide as a coenzyme, cultured at 35 deg.C for 20 days whereby the objective sodium normal-alkylbenzenesulfonate which is used as an anionic surfactant for detergent and thought to accumulate in sludge can be efficiently degraded.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a method for decomposing linear sodium alkylbenzene sulfonate. More specifically, it relates to a method for degrading sodium linear alkylbenzene sulfonate by a microorganism.

[0002]

2. Description of the Prior Art Sodium straight-chain alkylbenzene sulfonate is most often used today as an anionic surfactant contained in detergents, but it is more toxic to fish and aquatic organisms than soap and the like. There are some problems such as concern about the impact on aquatic organisms due to the accumulation of substances. On the other hand, sodium linear alkylbenzene sulfonate is said to be decomposed by microorganisms under aerobic conditions in rivers, but the amount of decomposition is small and the linear alkylbenzene sulfonic acid remaining without decomposition is Sodium is believed to accumulate in sludge.

[0003]

SUMMARY OF THE INVENTION An object of the present invention is to provide a method for decomposing a linear sodium alkylbenzene sulfonate by a microorganism.

[0004]

SUMMARY OF THE INVENTION The object of the present invention is as follows.
A microorganism having the ability to decompose sodium linear alkylbenzene sulfonate belonging to the genus Bacillus is added to a medium containing sodium linear alkylbenzene sulfonate, preferably a medium to which a coenzyme is further added, and the linear alkylbenzene is obtained by culturing. This is achieved by a method of decomposing sodium sulfonate. Examples of microorganisms belonging to the genus Bacillus and capable of decomposing sodium linear alkylbenzene sulfonate include Bacillus sp. No. E618-06 (FERM P-
15576) is used.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION Bacillus sp. No. E618-06 was isolated from soil in Aso-gun, Kumamoto Prefecture, and its culture was carried out using any medium under shaking conditions at 35 ° C. ~Ten
It takes place for about a day. Specifically, test tube with 3 ml of L-broth medium containing sodium linear alkylbenzene sulfonate (1% tryptone, 0.5% yeast extract, 0.5% NaCl, 0.05% sodium linear alkylbenzene sulfonate; pH 7.0 before sterilization). In L-broth agar medium (agar concentration 12
%) And one platinum loop of Bacillus sp. No. E618-06 colony is added, and the mixture is cultivated by shaking at 35 ° C for 24 hours.

This microorganism has the following mycological properties. A. Morphology (1) Cell shape and size Bacilli, 0.5 × 1 μm (2) No motility (3) Spore formation (4) Gram stain positive B. Growth state in medium Broth agar plate culture Yellowish brown, with ridge C. Physiological properties (1) With reduction of nitrate (2) Without VP test (3) No formation of indole (4) No generation of hydrogen sulfide (5) Utilization of citric acid (6) No formation of dye (7) Urease No production (8) With oxidase activity (9) No decomposition of lysine (10) No decomposition of ornithine (11) No decomposition of arginine (12) Growth range pH 4-8, temperature 25-40 ° C (13) Oxygen Attitude Aerobic (14) Acid production from sugars Glucose-mannitol-adnitol-arabinose-inosit-rhamnose-sorbit-maltose-sucrose-medium: peptone 2g, NaCl 5g, K ₂ HPO ₄ 0.3g, carbohydrate 10
g, bromthymol blue 0.08g, agar 15g, distilled water 100ml
(pH7.1) Additive concentration: 1%

Based on the above-mentioned mycological properties, this strain was designated as B
As a result of searching by ergey's Mannual of Determinative Bacteriology 9th edition, it was confirmed to be a bacterium belonging to the genus Bacillus .

[0008] Degradation of sodium linear alkylbenzene sulfonate using this bacterium is about 0.001-0.1
%, Preferably 0.05% sodium linear alkylbenzene sulfonate (Tokyo Kasei Product) -containing L-broth medium (121 ° C, 1.
Bacillus sp. No. E618-06 (FER), which had been pre-cultured in 10 ml of L-broth medium (sterilized at 1 kg / cm ² for 15 minutes)
M P-15576) is added in an amount of 10 ⁸ to 10 ¹² cells / ml, and the mixture is cultured under shaking conditions at 35 ° C. for about 20 days.

When culturing the microorganism in this way, along with sodium linear alkylbenzenesulfonate in the medium,
Degradation of sodium linear alkylbenzene sulfonate can be further promoted by adding about 0.1 to 10 mM, preferably about 0.5 to 5 mM of nicotinamide adenine dinucleotide reduced form and coenzyme such as ubiquinoline quinone.

[0010]

INDUSTRIAL APPLICABILITY Sodium linear alkylbenzene sulfonate can be decomposed by a single microorganism, and the addition of coenzyme can further accelerate the decomposition of sodium linear alkylbenzene sulfonate.

[0011]

Next, the present invention will be described by way of examples.

Example 1 10 ml of a culture medium (0.57% Na ₂ HPO ₄ , 0.386% NaH ₂ PO ₄ ; pH 7.0 before sterilization) supplemented with 0.05% sodium linear alkylbenzene sulfonate was added to Bacillus sp. E618-06 to 10 ¹¹
The cells were added under the condition of shaking at 140 rpm, 35 ° C., and 20 days. The culture broth was centrifuged at 6000 rpm for 5 minutes to collect the cells, and the supernatant was sterilized using a 0.2 μm filter, and the presence or absence of sodium linear alkylbenzene sulfonate was confirmed by capillary electrophoresis. .
For comparison, culturing was carried out without the addition of microorganisms, and a comparison was made with the one in which the presence or absence of linear alkylbenzene sulfonate was similarly confirmed. The sodium peak was not observed when microorganisms were added, and it was confirmed that the linear sodium alkylbenzenesulfonate was completely decomposed.

Example 2 0.003 g of sodium linear alkylbenzene sulfonate, (N
H ₄ ) ₂ SO ₄ 1 g, KH ₂ PO ₄ 10 g, KOH 1.94 g, MgSO ₄ 0.1 g, sodium citrate 0.5 g and nicotinamide adenine dinucleotide reduced form (Sigma) 0.075 g liquid medium 27 ml In addition, one platinum loop of a single colony of Bacillus sp. No. E618-06 was added to L-broth medium containing 0.01% sodium linear alkylbenzene sulfonate at 35 ° C, 2
When 3 ml of the culture solution which had been cultivated for 4 hours was added and shake culturing was carried out at 35 ° C., the number of days until sodium linear alkylbenzenesulfonate could not be detected was 8 days.

Example 3 In Example 2, the same amount of ubiquinoline quinone (manufactured by Sigma) was used in place of the reduced form of nicotinamide adenine dinucleotide, and the same culture was carried out. It was 10 days before I could not be detected.

When the same culture was carried out without the addition of coenzyme, the number of days until the detection of sodium linear alkylbenzenesulfonate could not be detected was 20 days.

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Claims (2)

[Claims] 1. A sodium linear alkylbenzene sulfonate characterized by comprising adding a microorganism belonging to the genus Bacillus and capable of degrading sodium linear alkylbenzene sulfonate to a medium containing sodium linear alkylbenzene sulfonate and culturing the same. Disassembly method. 2. The method for degrading sodium linear alkylbenzene sulfonate according to claim 1, wherein a medium supplemented with coenzyme is used.