JPH09275843A - Suspended substance removing device and suspended substance removing method using the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a suspended substance removing device which effectively and easily collect useful water living bodies when the useful water living bodies such as rotatoria are cultivated in a large quantity and in a high density. SOLUTION: This suspended substance removing device is manufactured by machining a contact filter material where suspended substances are made to stick to a large surface area, in a shape which does not impede the stirring of cultivating water, and this suspended substance removing device is arranged in cultivating water tank for useful water living bodies such as rotatoria to be cultivated, or the useful water living bodies to be cultivated are made to flow in the cultivating water to make the suspended bodies on the surface of the device. The device on which the suspended bodies stick is taken out of the cultivating water tank and the sticking bodies are washed away to efficiently and easily remove the suspended bodies and also easily and efficiently collect the useful living bodies. The device after the removal of the stuck suspended bodies is arranged in the cultivating water tank and reused again for suspended body removal.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an apparatus for removing suspended matter and a method for removing suspended matter using the apparatus, and more particularly, suspension produced during the cultivation of useful organisms in water such as rotifers. The present invention relates to a substance removing device and a suspended substance removing method using the device.

[0002]

2. Description of the Related Art Especially rotifers (eg Rotifer Brachionus)
Underwater useful organisms such as rotundiformis) are very important as an initial feed for seed production of seafood.

At present, rotifers are cultivated by feeding fresh water chlorella, nannochloropsis, yeast and the like. The rotifer culture method is, for example,
In this method, nannochloropsis is cultivated in an aquarium, to which supplementary feed such as freshwater chlorella or baker's yeast is supplemented as a main feed, and culturing is performed while supplementing air. However, according to this method, the culture density is in the range of about 50 to 500 individuals per 1 ml of culture water, and the growth rate is as low as about 20 to 30%, which is not practical. Therefore, in order to practically cultivate large numbers of rotifers by such a method, a rotifer culture tank of several hundred tons and a Nannochloropsis culture tank several times its size are required, and an extremely huge facility, It requires a vast amount of land to build the equipment and a huge investment to build it. Furthermore, in this culturing method, since the culture of Nannochloropsis depends on the weather, the culture of rotifers depends on the culture of Nannochloropsis, and thus on the weather, and the culture of rotifers becomes extremely unstable. There is also the drawback that

Therefore, in the method of feeding rotifers in an aquarium, the present inventors aerated oxygen to keep the concentration of dissolved oxygen DO in water at least 2 ppm, and
We have succeeded in developing a method for cultivating rotifers in high density and in large quantities by adjusting the pH of the water in the aquarium so as to always maintain the pH within the range of 5 to 7.5 (JP-A-7-79659). issue). In this culture method, 1m of culture water
Rotifers can be cultured at a high density of several thousand to 10,000 individuals per liter.

However, in such a method for cultivating rotifers at a high density and in a large amount by feeding such concentrated freshwater chlorella, suspended substances such as chlorella residues, insoluble organic substances and microorganisms increase in the culture water, These clog the collection nets used to collect rotifers, which is a major obstacle to the collection of rotifers.

[0006]

SUMMARY OF THE INVENTION Therefore, the present invention provides a method for easily and efficiently recovering rotifers in order to efficiently and easily recover rotifers when mass-culturing rotifers at high density. It is an object of the present invention to provide a suspended matter removing device that can remove the suspended matter.

Another object of the present invention is to provide a method of removing suspended matter which can remove suspended matter efficiently and easily by using such a suspended matter removing apparatus.

In order to achieve the above-mentioned object, the suspended substance removing apparatus according to the present invention is characterized in that the contact filter medium to which the suspended substance is adhered is formed in a shape having a large surface area.

The method for removing suspended matter according to the present invention is
A suspended matter removing device in which the contact filter medium to which suspended matter is attached is formed to have a shape having a large surface area,
A useful aquatic organism that is placed in a culture water tank for cultivating a useful aquatic organism and operates the suspended substance removing device, or attaches and removes a suspended substance while flowing the culture water in the culture aquarium. And the suspended substance removing device is removed from the culture water tank after the culture, and the suspended substance adhering from the suspended substance removing device is removed to remove the suspended substance.

The suspended matter removing apparatus according to the present invention is formed by cultivating useful aquatic organisms such as rotifers, for example, by forming a contact filter medium such as a nylon mat into a shape having a large surface area. It is installed in the aquarium. By rotating this suspension substance removal device in a culture water tank at an appropriate speed, or by causing the culture water to stir or aerate at an appropriate speed to flow, it is possible to increase the useful organisms in water such as rotifers. While the part is prevented from adhering to the suspended substance removing device, while the suspended substance is attached to the surface of the suspended substance removing device, the suspended substance in the culture water is efficiently and easily removed, and Underwater useful organisms such as rotifers in the culture water can be easily and efficiently recovered.

In addition, as described above, the suspended substance removing device to which the suspended substance in the culture water is attached adheres to the surface of the suspended substance removed by removing the suspended substance from the culture water tank and washing with water or the like. Can be washed off and easily removed.

Further, the suspended substance removing device in which the suspended substances adhering to the surface thereof are washed off is installed again in the culture water tank and can be repeatedly used to remove the suspended substances. There is also an advantage.

Furthermore, by using the suspension removing apparatus according to the present invention, the scale of the culture tank for cultivating useful aquatic organisms such as rotifers can be made extremely small.

Therefore, in the present invention, it is possible to more efficiently cultivate underwater useful organisms such as rotifers by using a small culture water tank, and it is possible to easily and efficiently recover the organisms. is there.

[0015]

In the suspended substance removing device according to the present invention having the above-described action, for example, a contact filter medium such as nylon is molded into a shape having a large surface area. The contact filter material is not particularly limited in its material, and the suspended matter to be removed is attached to it, but on the other hand, a minute through hole is provided so that useful aquatic organisms such as rotifers can swim even if attached. It is preferable that it is in the form of a net or a net, and that it does not have any adverse effect on such useful aquatic organisms. In addition, even if the size of the through hole or net is large, suspended matter that you want to attach and remove does not pass through, but it is necessary that useful aquatic organisms such as culture water and rotifers can actively swim. . If the size of the mesh of the through hole or net is too small, suspended substances will adhere and block the mesh of the through hole or net, and free flow of useful water organisms such as culture water flow and rotifers. It is not preferable because it interferes with swimming, resulting in the culturing of useful aquatic organisms such as rotifers.

The suspended matter removing apparatus according to the present invention is
It is preferable to mold the above-mentioned contact filter medium, for example, a nylon filter medium, into a shape having a large surface area so that suspended substances can be easily attached. In the suspended matter removing device according to the present invention, the shape of increasing the surface area is not particularly limited, but for example, a shape of a hollow polygonal column (for example, a hollow column having a star (*) cross section) In addition, it is preferable to form the contact filter media in a radial arrangement, or to form the contact filter media in parallel in one row or several rows.
The method of processing the contact filter medium to be used into such a shape is not particularly limited, for example, by assembling a frame made of stainless steel or aluminum into a polygonal shape,
Contact filter media are attached to the frame, stainless steel or aluminum frames are radially assembled and the contact filter media are radially attached, or stainless steel or aluminum frames are arranged in one or several rows of contact filter media. It is also possible to assemble them so that they can be attached to each other and to form the contact filter media by attaching them in parallel in one row or in several rows.

The suspended substance removing device produced as described above is installed in a water tank for cultivating useful aquatic organisms such as rotifers. In order for the suspended matter removing device according to the present invention to be able to efficiently and easily recover useful aquatic organisms such as rotifers, it is possible to remove suspended substances efficiently. It is possible to move the culture water at an appropriate speed by moving the culture water at an appropriate speed, stirring the culture water in the culture water tank, or aeration of oxygen in the culture water. Good. In addition, the appropriate speed for flowing the culture water varies depending on the size of the culture tank and the amount of culture water, so even if useful aquatic organisms such as rotifers passively attach to the contact filter medium, they will be active. The suspension removing device or the culture water may be set to any condition as long as the speed is such that the adhesive filter medium can swim out. If the speed of moving the suspension remover or moving the culture water is too high, once useful aquatic organisms such as rotifers are attached to the suspension remover, the device will be removed. They will not be able to actively leave and eventually die.

Further, the suspended substance removing apparatus according to the present invention is
Although it largely depends on the flow rate of the culture water, it is generally preferable that the culture water is placed in the culture water tank so as to be substantially parallel to the flow direction of the culture water. If the suspended solid removal device is placed in the culture water tank so as to be perpendicular to the flow direction of the culture water, useful aquatic organisms such as rotifers will easily adhere to the contact filter medium and will be activated from the contact filter medium. There is a risk that you will not be able to swim and leave. Even in this case, as long as the flow rate of the culture water is slow and does not affect the removal of useful aquatic organisms such as rotifers from the contact filter medium, the suspended matter removing device is placed in the flow direction of the culture water. It can also be arranged vertically in the culture water tank.

The suspended substance removing device having the above-mentioned structure can be easily taken out from the culture water tank, and after taking out, the attached suspended substances can be easily washed off and removed by washing with water. The suspended substance removing device, which has washed away and removed the suspended substances in this way, can be installed again in the culture water tank and used to remove the suspended substances.

A large amount of useful aquatic organisms such as rotifers can be easily recovered from the culture water after the suspended substance removing device is taken out from the culture water tank as described above.

[0021]

BEST MODE FOR CARRYING OUT THE INVENTION Next, with reference to the drawings, an outline of an example of a culture water tank in which a suspended matter removing apparatus according to the present invention can be installed will be described.

As shown in FIG. 1, the culture water tank comprises a water tank 1 using an Artemia hatcher, and culture water 2 made of filtered seawater (pH 8.2) is put in the water tank. The water tank 1 is provided with an oxygen disperser 3 for discharging oxygen into the culture water 2 in the form of small bubbles and an air discharger 4 for discharging air bubbles into the culture water 2. A small oxygen generator 5 is arranged in the oxygen disperser 3 and supplies oxygen to the oxygen disperser 3. An air introducing pipe 6 is connected to the air ejector 4 and is arranged so as to introduce air into the air ejector 4 from the outside of the water tank 1. Further, a fresh water chlorella storage container 9 is disposed in the water tank 1, and a fixed amount fresh water chlorella 7 serving as a feed for rotifers and the like is supplied to the culture water 2 by a fixed amount pump 10. Further, a titanium heater 11 is provided in the culture water 2 in the water tank 1 so that the temperature of the culture water 2 can be adjusted. Note that reference numeral 12 indicates a control unit of the titanium heater 11. Further, in the water tank 1, a suspended substance removing device 13 according to the present invention is arranged in a state of being suspended in the culture water 2. Further, in the figure, reference numeral 15
Is a pH adjusting agent container containing the dilute hydrochloric acid solution 14 as a pH adjusting agent, and 16 is a metering pump for supplying the dilute hydrochloric acid solution 14 into the culture water 2.

FIG. 2 shows an example of the suspended substance removing apparatus according to the present invention. In FIG. 2, the suspended solid removing device has a structure in which a stainless steel frame 30 is assembled so that its cross section has a star shape, and a mat 20 made of a contact filter material such as nylon is attached to the outer surface thereof. ing.

Further, FIG. 3 shows another example of the suspended substance removing apparatus according to the present invention. In FIG. 3, the suspended substance removing device is shown as an inner upper frame 30 assembled concentrically.
A stainless steel frame composed of a double ring-shaped upper frame portion 30 composed of a and the outer upper frame 30b and a lower frame composed of double rings assembled in the same concentric shape is contacted with, for example, nylon. The mat 20 made of a filter medium is radially mounted between the double rings.

For example, when a suspension removing device having a shape as shown in FIG. 2 or 3 is installed in a culture water tank, the suspension removing device is generally installed at the center of the culture water tank. Arranged, ventilating oxygen etc. from the lower part of the central part,
It is preferable that the culture water is allowed to flow between the adjacent mats 20 by flowing from the bottom to the top.

Furthermore, FIG. 4 shows another example of the suspended substance removing apparatus according to the present invention. In FIG. 4, the suspended solid removing device has, for example, a structure in which a mat 30 made of a contact filter material such as nylon is attached in parallel to one another or in a plurality of rows on a frame 30 made of rectangular stainless steel. There is. In the case of the suspension removing apparatus having such a shape, generally, the mat 20 is placed in a rectangular culture water tank in which the culture water can be horizontally flowed and cultured so that the mat 20 is substantially horizontal. It is preferable that the culture water flow on the upper surface and the lower surface of the mat.

The suspended substance removing apparatus according to the present invention and its effects will be described below with reference to examples. In addition,
The present invention is not limited to the examples described below, and it is understood that the following examples are merely examples for explaining the suspended matter removing device according to the present invention. It should be.

[0028]

【Example】

Example 1 In this example, the rotifer (Brachi
nonus rotundiformis, length of armor, 190 ± 14 mm) was used. As a feed, commercially available concentrated freshwater Chlorella
regularis, manufactured by Nisshin Fine Chemical Co., Ltd., cell density:
150x108 individual / ml, PCV: 600 ml / l, hereinafter referred to as chlorella) was used.

In this example, a culture water tank 1 equipped with a suspended matter removing device as shown in FIG. 2 was used. This suspended matter removing device is, for example, a stainless steel frame (inner diameter 20
cm, outer diameter 60 cm, height 40 cm) with a contact filter material (nylon mat, trade name: Vilene mat) attached. The suspended substance removing device used was a hollow polygonal one, for example, one whose cross section was processed into a star (*) shape.

The density of fresh water chlorella was counted three times under a microscope using a hemocytometer.

The rotifer density is 100 per 1 ml of culture water.
It was diluted with seawater so as to be less than the number of individuals, and 1 ml thereof was collected and counted 6 times under a stereoscopic microscope.

In this example, two surfaces of a 500-liter water tank were used, one of which was a suspension substance removal device installation section (test section) and the other was a non-installation section (target section). The suspended matter removing device in the installation area was taken out of the water tank and washed after collecting rotifers every morning. The culture was initially carried out by a thinning method in which 4000 rotifers / ml were inoculated and the growth was collected from the next day, and the experimental period was 5 days. For other culture methods, oxygen gas was aerated (1-5 liters / minute), the pH was controlled to pH 7 and the culture water temperature was 32 ° C. according to a known method. Hydrochloric acid (7%) as a pH adjuster
Was used. The feeding amount of Chlorella was O.5 liter / 100 million individual rotifers, and this amount was fed by a metering pump for about 20 hours. In addition, the amount of fresh food chlorella remaining in the culture water was counted every morning. The amount of suspended solids in the culture water was measured by a known method every morning by taking 1 ml of culture water from both sections into 6 centrifuge tubes before collecting the rotifer.
Spinning was performed for 30 minutes at 00 rotation and PV (Packed v
olume). The amount of deposits on the suspended matter removal device is about 5 every morning after picking up and cleaning the suspended substance removal device.
It was allowed to stand for a minute, its weight was measured, and the difference was calculated.

The amount of residual feed of freshwater chlorella in the culture water remained in the range of 2 to 9 × 10 ⁴ cell individuals / ml in the setting area. The amount of the suspension adhered to the suspended matter removing device was in the range of 1.9 to 3.3 kg.

In the non-installed section, a large number of Chlorella cells were present in the flocs of the suspended substance, so that counting was not possible.

When collecting and washing the rotifer, the collection net was not clogged during the culture period in the installation area. On the other hand, in the non-installed section, the cells were clogged from the day after the culture, and in order to recover a predetermined amount, the cells had to be collected and washed in 3 to 4 times, and a large suspended substance remained after the washing. .

In addition, since the rotifers could be collected and washed without any trouble in the installation area, this flocculated suspended substance is the main cause of clogging the recovery net which constitutes the suspended substance removing apparatus of the present invention. it is conceivable that.

With reference to FIGS. 5 to 8, the effect of installing the suspended matter removing device in this embodiment will be described. FIG.
And FIG. 6 shows the case where a suspended matter removing device is installed. Further, FIGS. 7 and 8 show the case where the suspended matter removing device is not installed. As shown in FIGS. 5 and 7, it is apparent that there is almost no difference in the rotifer density between the case where the suspended substance removing device is installed and the case where it is not installed. However, as shown in FIG. 6, when the suspended matter removing device is installed, the suspended matter such as microorganisms and dirt and the rotifer are separated into layers,
The rotifer can be easily separated from the suspension, whereas as shown in FIG. 8, when the suspended matter removing device is not installed, the suspension of the microorganisms and dirt and the rotifer can be separated. It was impossible to easily separate the rotifer from the suspension because they were mixed and integrated.

[Brief description of drawings]

FIG. 1 is a schematic view of a culture water tank in which a suspended matter removing device according to the present invention can be used.

FIG. 2 is a perspective view showing an example of a suspended matter removing device according to the present invention.

FIG. 3 is a plan view showing another example of the suspended substance removing apparatus according to the present invention.

FIG. 4 is a plan view showing still another example of the suspended substance removing apparatus according to the present invention.

FIG. 5 is a graph showing the rotifer density when the suspension substance removing apparatus according to the present invention according to Example 1 is attached and cultured.

FIG. 6 is a graph showing the experimental results when the suspension substance removing apparatus according to the present invention according to Example 1 was attached and cultured.

FIG. 7 is a graph showing the rotifer density in the case of culturing without the suspension substance removing apparatus according to the present invention according to Example 1 being attached.

FIG. 8 is a graph showing the experimental results in the case of culturing without the suspension substance removing device according to the present invention according to Example 1.

[Explanation of symbols]

 1 Water Tank 13 Suspended Substance Removal Device 20 Mat

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[Procedure amendment]

[Submission date] May 16, 1996

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] Claim 1

[Correction method] Change

[Correction contents]

[Procedure amendment 2]

[Document name to be amended] Statement

[Correction target item name] Claim 2

[Correction method] Change

[Correction contents]

[Procedure 3]

[Document name to be amended] Statement

[Correction target item name] 0008

[Correction method] Change

[Correction contents]

In order to achieve the above-mentioned object, the apparatus for removing suspended matter according to the present invention is formed so that the contact filter medium to which suspended matter adheres has a large surface area and does not inhibit stirring of culture water. It is characterized by that.

[Procedure amendment 4]

[Document name to be amended] Statement

[Correction target item name] 0009

[Correction method] Change

[Correction contents]

The method for removing suspended matter according to the present invention is
The contact substance filter to which the suspended substance is attached has a large surface area and is shaped so as not to hinder the stirring of the culture water.The suspended substance removing device is arranged in the culture water tank for cultivating useful organisms in water. , Moving the suspension substance removing device, or culturing useful organisms in water while adhering and removing the suspended substance while flowing the culture water in the culture water tank, The suspended substance is removed by removing it from the culture water tank and removing the suspended substance adhering from the suspended substance removing device.

Claims (5)

[Claims] 1. An apparatus for removing suspended solids, characterized in that a contact filter medium to which suspended solids are attached is formed to have a shape having a large surface area. 2. A suspended matter removing device, which is formed by shaping a contact filter medium to which suspended matter adheres to have a shape having a large surface area, is disposed in a culture water tank for cultivating useful aquatic organisms, and the suspension is provided. The turbid substance removing device is moved, or the useful substance in water is cultured while the suspended substance is adhered and removed while flowing the culture water in the culture water tank, and after the culturing, the suspended substance removing device is cultivated. A method for removing suspended matter, which comprises removing the suspended matter by removing it from the water tank and removing the suspended matter adhering from the suspended matter removing apparatus. 3. The method for removing suspended solids according to claim 2, wherein the suspended solids are passively attached to the contact filter medium, but the aquatic useful organisms are active even when attached to the contact filter medium. To move the suspended matter removing device or to make the culture water flow to such an extent that the subject can swim. 4. The method for removing suspended substances according to claim 2 or 3, wherein the suspended substance removing device is arranged in the culture water tank so as to be substantially parallel to the flow direction of the culture water. To do. 5. The method for removing suspended matter according to any one of claims 2 to 4, wherein the culture water is adjusted to pH 5 to pH 5.
Adjust to 7.5.