JPH09165312A - Microbial material for cultivating crop - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a microbial material, improved in preservation stability and useful for cultivating crops so as to stably provide a high production efficiency. SOLUTION: This microbial material for cultivating crops comprises a carrier containing water and a coal ash and a soil microorganism containing at least a bacterium belonging to the genus Azospirillum. The microbial material contains 2×10<8> to 10<12> individuals of the bacterium belonging to the genus Azospirillum based on 1g carrier. The content of the coal ash in the carrier is 3-75wt.% based on the total amount of the carrier and the moisture content based on the total amount of the carrier is 50-90wt.%.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a microbial material for cultivating crops, and more particularly to a microbial material for cultivating crops containing a bacterium belonging to the genus Azospirillum, the content of which is adjusted and which is excellent in storage stability.

[0002]

2. Description of the Related Art In recent years, attempts have been made to improve the production of agricultural products by utilizing microorganisms that act beneficially on plants. Representative examples of such microorganisms include root nodule bacteria, bacteria belonging to the genus Pseudomonas, and bacteria belonging to the genus Azospirillum. Among them, a bacterium belonging to the genus Azospirillum is one of plant growth-promoting rhizobacteria known to loosely co-exist with plants and promote plant growth. Inoculate crops with a pure culture of this bacterium. Attempts have been made to increase the yield, and to inoculate the legumes with this bacterium at the same time as the root nodule bacteria to increase the yield compared with the case of using the root nodule alone. To actually inoculate a crop with the bacterium belonging to the genus Azospirillum, after culturing the microbial cells, usually soil such as Kanuma soil or porous stone is used as a carrier, and the microbial cells are added to this. Generally, a method of producing a microbial material to which the culture of (1) is adsorbed and applying the microbial material to soil for cultivating a crop or the like is used.

However, in some of these microbial materials, the effective activity thereof declines with time, and the effective activity disappears as the storage period becomes longer. That is, it is not always possible to obtain a microbial material having a stable effective activity, and therefore there is a problem that the effect is not stable in a crop to which the microbial material is applied. In most cases, the maintenance of this effective activity is greatly influenced by the preservability of the bacterial cells contained in the microbial material. Therefore, in order to promote the practical use of the microbial material, it has been earnestly desired to develop a technique for improving the preservability of cells contained in the microbial material and stabilizing the effective activity of the microbial material.

[0004]

SUMMARY OF THE INVENTION The present invention has been made from the above viewpoint, and provides a microbial material having improved storage stability for cultivating crops that can stably obtain high production efficiency. Is an issue.

[0005]

Means for Solving the Problems As a result of repeated studies to solve the above problems, the present inventor has found that a carrier containing water,
In a microbial material for crop cultivation containing at least a soil microorganism containing a bacterium belonging to the genus Azospirillum,
By setting the content of bacteria belonging to the genus Azospirillum to 2 × 10 ⁸ to 10 ¹² individuals per 1 g of the carrier, the microorganisms in the microbial material can be stably preserved, and the use of this microbial material enables efficient cultivation of crops. The inventors have found that the above can be achieved and have completed the present invention.

That is, according to the present invention, in a microbial material for crop cultivation containing a carrier containing water and a soil microorganism containing at least bacteria belonging to the genus Azospirillum, the content of the bacteria belonging to the genus Azospirillum is 2 per 1 g of the carrier. It is a microbial material for cultivating crops of 10 ⁸ to 10 ¹² individuals.

The carrier used for the microbial material for cultivating crops of the present invention (hereinafter, also simply referred to as "microbial material") may be a carrier which contains water and can carry soil microorganisms containing bacteria belonging to the genus Azospirillum. There is no particular limitation, and it is possible to produce it by using an organic material or an inorganic material usually used for a microbial material together with an optional component added as necessary.
These materials can be used alone or in combination of two or more kinds. Further, in the present invention, it is preferable that the carrier contains coal ash in an amount of about 3 to 75% by weight based on the total amount of the carrier. Here, the content of coal ash in the carrier refers to the content when the carrier material is in the raw material state, whether the carrier material already contains water in the raw material state, or is it dry. Regardless of the above, it means the content calculated by using the weight in that state as it is.

The carrier used in the present invention contains water, and the content thereof is specifically 50 to 9 with respect to the total amount of the carrier.
It is preferably about 0% by weight, more preferably 5
It is about 5 to 85% by weight. Here, the water content of the carrier in the microbial material of the present invention means the water content calculated by the following formula from the weight of the carrier before and after drying the carrier at 70 ° C. for 3 days.

[0009]

## EQU1 ## Moisture content (% by weight) = 100 × (weight before drying−weight after drying) / weight before drying

The bacterium belonging to the genus Azospirillum contained in the microbial material of the present invention is not particularly limited as long as it is a Gram-negative bacterium identified as a bacterium belonging to the genus Azospirillum, and specifically, Azospirillum lipoferrum. , Azospirillum blasiliens, Azospirillum halopreface, Azospirillum amazonense, and the like. As described above, the content of the bacterium belonging to the genus Azospirillum contained in the microbial material of the present invention is approximately 2 × 10 ⁸ to 10 ¹² individuals per 1 g of the carrier, preferably 4 × 10 ⁸ to 10 ¹¹ individuals, and more preferably 5 × 10 ^{8 to} 5 × 10 ¹⁰ individuals.

The microbial material of the present invention can also be mixed with soil microorganisms other than the bacteria belonging to the genus Azospirillum as soil microorganisms. Examples of such soil microorganisms include microorganisms that generally live in soil and are said to exert useful effects on plants. Further, in the microbial material of the present invention, in addition to the above-mentioned carrier and soil microorganisms, it is also possible to mix optional components usually contained in the microbial material.

The microbial material for cultivating a crop of the present invention is used for cultivating a crop by the same method as using a normal microbial material. The microbial material for cultivating crops of the present invention enhances the storability of microorganisms by adjusting the content of the bacteria belonging to the genus Azospirillum in the microbial material to 2 × 10 ⁸ to 10 ¹² individuals per 1 g of the carrier, thereby improving the storability of microorganisms. The effect is much more stable than microbial materials. Further, when a crop is cultivated using the microbial material of the present invention, the effect of microorganisms can be stably obtained, and efficient cultivation of the crop becomes possible.

[0013]

Embodiments of the present invention will be described below. First, the carrier used for the microbial material of the present invention, usually, it is possible to use an organic or inorganic material used in the microbial material as the main material, specifically,
Inorganic materials such as Akadama soil, fired Akatama soil, Kanuma soil, Kuroboku soil, vermiculite, perlite, zeolite, coal ash, peat moss, pulp, straw, bacas, oil cake, fish cake, bone meal, blood meal, crab, charcoal, Organic materials such as fossil shells can be used. These inorganic and organic materials can be used alone or as a mixture of two or more kinds for the carrier of the microbial material of the present invention. Further, various minor components such as lime, which is blended for pH adjustment described below, can be blended in the carrier as required.

Further, in the carrier used in the present invention, the pH when 5 parts by weight of water is added to 1 part by weight of the carrier (hereinafter, when simply described as pH, the pH measured under the above conditions is Is preferably in the range of 5.5 to 8.0. For example, some peat moss and the like have a pH of 5.5 or less. Therefore, when this is used alone, the pH is adjusted within the above range by adding additives such as lime usually used for pH adjustment in microbial materials. It is preferable to adjust so that Even when a material other than peat moss is used, the pH can be adjusted as necessary in the same manner as above.

The microbial material carrier of the present invention includes, for example,
One kind or two or more kinds of the above-mentioned various materials are used, but it is preferable that the carrier contains coal ash, specifically, pulverized coal combustion ash, fluidized bed combustion ash pulverized powder and the like. Coal ash that is preferable as coal ash to be added to the carrier of the microbial material of the present invention has a calcium oxide content of 0.5% by weight or more and an average particle size of 5 mm or less. As the coal ash, more preferably, particles having a particle size of 3 mm to 10 μm occupy 80% or more of the entire coal ash. Further, as the coal ash used in the present invention, one having a pH of 9 or more when 5 parts by weight of water is added to 1 part by weight of the coal ash is more preferable. Further, the content of coal ash in the carrier is preferably 3 to 75% by weight based on the total amount of the carrier in dry weight. Further, when coal ash is added to the carrier as described above, it is preferable that the pH of the carrier is in the range of 5.5 to 8.0.

By incorporating water into such a carrier, the carrier used for the microbial material of the present invention can be obtained. Or,
Water can be added after mixing the microorganisms with the above carrier. In addition, a carrier that already contains water in a normal state can be used as it is for the microbial material of the present invention. The appropriate amount of water contained in the carrier used in the present invention is as described above.

In addition to the above carrier, soil microorganisms containing at least bacteria belonging to the genus Azospirillum are blended with the microbial material of the present invention. Specific examples of the bacterium belonging to the genus Azospirillum are as described above, and more specifically, as Azospirillum lipoferrum, Azospirillum lipoferrum ATCC29709 and the like are used as Azospirillum fragilis.
Brasilens ATCC 29145, Azospirillum
Brasilence ATCC 29710 and the like can be mentioned as Azospirillum halopreferrance, Azospirillum halopreferrance ATCC43709 and the like, and Azospirillum amazonense and Azospirillum amazonense ATCC 35119 and the like. The bacterium belonging to the genus Azospirillum used in the present invention is not limited to these.

The soil microorganisms other than the above-mentioned bacteria belonging to the genus Azospirillum are not particularly limited as long as they are microorganisms that generally live in soil and exert a useful effect on plants. Genus, Azolyzobium, Pseudomonas, Rhizobium, Bacillus, Bradyrizobium, Agrobacterium, Streptomyces, Xanthomonas, Lactobacillus, Aeromonas, Anabena, Francia, Rhodopseudomonas, Trichoderma , Genus Glomus, genus Aspergillus, genus Penicillium, genus Rhizopus, genus Fusarium, genus Gliogladium, genus Gigaspora, genus Scutellospora, genus Nostok, or genus Azotobacter.

Further, one of these microorganisms may be used in the microbial material of the present invention together with a bacterium belonging to the genus Azospirillum, or a combination of two or more of these microorganisms may be used with a bacterium belonging to the genus Azospirillum. is there. When a bacterium belonging to the genus Azospirillum is used in combination with these microorganisms, it is preferable to appropriately select the combination and the blending amount that do not impair the usefulness of each microorganism for plants.

When these microorganisms are used in the present invention, as in the case of using microorganisms for ordinary microbial materials,
It is preferable to use a culture obtained by culturing the cells in a medium in which the cells can grow. For example, for bacteria belonging to the genus Azospirillum, RC medium (L-malic acid: 5 g /
L, KOH: 4.8 g / L, yeast extract: 0.5 g /
_{L, KH 2 PO 4: 0.5g} / L, MgSO 4 · 7H 2 O:
0.2 g / L, NaCl: 0.1 g / L, FeCl ₃ ·
6H ₂ O: 0.015 g / L, pH 7.0) and the like can be used to obtain a culture of bacterial cells by culturing under ordinary culture conditions, for example, at 25 to 37 ° C. for 2 to 3 days.

The culture of the bacterial cells thus obtained is used in the present invention as it is, or after separating the bacterial cells from the culture by centrifugation or the like, or after drying the culture or the bacterial cells. Is possible.

The mixing ratio of the microorganism and the water-containing carrier in the microbial material of the present invention is as described above for the bacterium belonging to the genus Azospirillum. For other microorganisms, it is superior to cultivated crops that the total number of cells of the microorganisms excluding the bacteria belonging to the genus Azospirillum is approximately 0.1 to 10 times the number of the cells of the bacteria belonging to the genus Azospirillum. In order to obtain a growth promoting effect, it is preferable.

A method for cultivating a crop using the microbial material for cultivating a crop of the present invention obtained as described above, may be carried out in the same manner as using an ordinary microbial material. Specifically, the following method can be mentioned.

As a method of applying the microbial material to the cultivation soil, when the seedlings of the crop are grown or the crop is cultivated using a pot, a cell or the like, an appropriate amount of the microbial material is mixed into the cultivation soil to obtain a uniform mixture. Examples thereof include a method of mixing and sowing seeds of a crop there, or planting seedlings, cuttings, sowing tanemo, and the like. When cultivating crops in the field, a suitable amount of microbial material is uniformly mixed with the soil (cultivation soil) that covers the rhizosphere of the crop, and seeds are sown and seedlings are transplanted. A general method is a method of performing.

When the microbial material is applied to the cultivation soil, the application amount of the microbial material is 10 ³ to 1 as the number of bacteria belonging to the genus Azospirillum per 1 g of dry weight of the cultivation soil.
It is preferable to apply so as to contain 0 ⁷ pieces, and more preferably 10 ⁵ to 10 ⁷ pieces / g dry soil. If the application rate is less than 10 ³ pieces / g dry soil, the growth promoting effect of the crop tends to be unstable, and if the application rate exceeds 10 ⁷ pieces / g dry soil, the effect will reach a ceiling and will be economical. Not preferable.

As a base material for cultivation soil, a general material,
For example, one or more selected from field soil, rice soil, mountain soil, sand, red jade soil, charcoal, zeolite, perlite, vermiculite, Kanuma soil, pumice stone, coral sand and the like can be mentioned. Of these, the preferred substrate is artificial soil or sand in which several of the above materials are combined. Further, in addition to the above-mentioned base material, it is possible to mix various components according to various purposes in the cultivation soil, and for example, a slow-release fertilizer is often mixed.

The crop to which the microbial material for crop cultivation of the present invention is applied is not particularly limited, but specifically,
Examples include crops selected from legumes, brassicaceae, asteraceae, araceae, seriaceae, lilies, grasses, roses and the like. Among these, broad beans, peanuts, azuki beans,
Soybeans, green peas, green beans (above, legumes), komatsuna, cabbage, cabbage, radish, turnip, cauliflower, broccoli (above, cruciferous crops), lettuce, shungiku, burdock (above, asteraceae), taro, konjac. (Above, Araceae crops), celery, carrots (above, Aeriaceous crops), yams, leeks, onions, asparagus (above, Lilyaceae crops), corn (Poaceae crops), strawberries (Rosaceae crops), etc. The crop can be mentioned as a crop that can obtain a remarkable effect when cultivated using the microbial material of the present invention.

[0028]

Embodiments of the present invention will be described below. First, a production example of a bacterial cell culture used for the microbial material of the present invention will be described.

[0029]

[Production Example] Cultivation of bacterial cells As a bacterium belonging to the genus Azospirillum, Azospirillum brasilens ATCC 29145 was used.
C medium (L-malic acid: 5 g / L, KOH: 4.8 g /
L, yeast _{extract: 0.5g / L, KH 2 PO} 4: 0.5g
_{/ L, MgSO 4 · 7H 2} O: 0.2g / L, NaCl:
_{0.1g / L, FeCl 3 · 6H} 2 O: 0.015g /
L, pH 7.0) and cultured at 32 ° C. for 48 hours. After the culture was completed, the cells were collected by centrifugation.

In addition, in the examples described below, a bacterium belonging to the genus Bradyrizobium (Bradyrizobium japonicum ATCC 10324) was used as a soil microorganism in addition to the bacterium belonging to the genus Azospirillum. Bradyrizobium japonicum ATCC 103
24 to the medium (mannitol: 10 g / L, yeast extract:
0.4 g / L, KH ₂ PO ₄ : 0.5 g / L, MgSO ₄
・ 7H ₂ O: 0.2 g / L, NaCl: 0.1 g / L)
Was inoculated and cultured at 32 ° C. for 48 hours. After the culture was completed, the cells were collected by centrifugation.

[0031]

Examples 1 and 2 Vermiculite having a water content adjusted to 55% was used as a carrier, and the bacteria belonging to the genus Azospirillum obtained in the above Production Example were added to the carrier in an amount of 10 ⁹ per 1 g of the carrier.
Three kinds of microbial materials were prepared by mixing and mixing uniformly so as to obtain 10 ¹⁰ individuals. The obtained microbial materials were used as the microbial materials of Example 1 and Example 2, respectively.
In addition, the wet bacterial cell itself obtained by the above Production Example, in which nothing was added to the wet bacterial cell of the bacterium belonging to the genus Azospirillum, was used as the microbial material of Comparative Example 1. Furthermore, the same carrier as used in the above Examples was mixed with the bacterium belonging to the genus Azospirillum obtained in the above Production Example so that 10 ⁶ or 10 ⁷ individuals per 1 g of the carrier were mixed and uniformly mixed. The microbial materials of 2 and Comparative Example 3 were prepared.

<Preservation test of bacteria belonging to the genus Azospirillum> The microbial materials of each of the above Examples and Comparative Examples were placed at the temperatures shown in Table 1, and after 240 days, the survival number of the bacterial cells was measured by the following method. The survival rate was calculated. 1 of microbial material
g was collected, suspended in 30 ml of 0.1 M MgSO ₄ and shaken for 1 hour, and the suspension was diluted 10000 times to prepare a standard agar medium (composition; yeast extract: 2.5 g / L, peptone:
5.0 g / L, glucose: 1.0 g / L, agar: 1
The number of bacteria at the time of culturing was measured using 5.0 g / L, pH; 6.0 to 7.0). 24 of the bacteria belonging to the genus Azospirillum in the microbial material of each Example and each Comparative Example
The survival rate after 0 days is shown in Table 1.

[0033]

[Table 1]

From these results, as compared with the microbial material of the comparative example in which no carrier is used or the content of the bacterium belonging to the genus Azospirillum is outside the scope of the present invention, the microbial material of the example contains the genus Azospirillum. It can be seen that the bacterium belonging to the group has a very good preservation property.

[0035]

Examples 3 to 5 Vermiculite having a water content adjusted to 55% was used as a carrier, and 10 ⁹ individuals of the bacterium belonging to the genus Azospirillum and the bacterium belonging to the genus Bradyrizobium obtained in the above production example were used per 1 g of the carrier. The microbial material of Example 3 was prepared by blending as described above and uniformly mixed. Similarly, each of the above-mentioned bacteria was blended so as to be 10 ¹⁰ individuals or 10 ¹¹ individuals per 1 g of the carrier, and uniformly mixed to obtain Example 4 (containing 10 ¹⁰ individuals / g) and Example 5.
A microbial material (containing 10 ¹¹ individuals / g) was prepared. In addition, the wet bacterial cell mixture itself obtained by uniformly mixing the wet bacterial cells of the bacterium belonging to the genus Azospirillum and the wet bacterial cells of the bacterium belonging to the genus Bradyrizobium obtained in the above Production Example is the microbial material of Comparative Example 3. And In the microbial material of Comparative Example 3, the bacterial cell concentration was 5 × 10 ¹¹ individuals /
g. Further, the same carrier as the carrier used in the above Examples was mixed with the bacterium belonging to the genus Azospirillum and the bacterium belonging to the genus Bradyrizobium obtained in the above Production Example so as to be 10 ⁶ or 10 ⁷ individuals per 1 g of the carrier, respectively. The mixture was uniformly mixed to prepare microbial materials of Comparative Example 4 (containing 10 ⁶ individuals / g) and Comparative Example 5 (containing 10 ⁷ individuals / g).

<Preservation test of each bacterium> The microbial materials of each of the above Examples and Comparative Examples were placed at the temperatures shown in Table 2, and after 240 days, the same method as the preservative test of the bacteria belonging to the genus Azospirillum was conducted. The survival rate was determined by measuring the number of surviving cells. Table 2 shows the survival rates of the bacteria belonging to the genus Azospirillum and the bacteria belonging to the genus Bradyrizobium after 240 days in the microbial materials of Examples and Comparative Examples.

[0037]

[Table 2]

From these results, the microbial material of the Example contains the azospirillum genus as compared with the microbial material of the comparative example in which no carrier is used or the content of bacteria belonging to the genus Azospirillum is outside the scope of the present invention. It can be seen that the bacterium belonging to the group has a very good preservation property. In addition, the bacteria belonging to the genus Bradyrizobium mixed with the bacteria belonging to the genus Azospirillum in the examples do not adversely affect the preservability of the bacteria belonging to the genus Azospirillum, and also the preservability of the bacteria themselves belonging to the genus Bradyrizobium. It turns out that is better.

[0039]

Examples 6 and 7 In Example 6, vermiculite having a water content adjusted to 50% was used as a carrier, and in Example 7, peat moss (lime was added to adjust pH to 5.5 to 6.
Peat moss adjusted to 0. All peat moss used below were peat moss treated in the same manner. )
1 part by weight of coal ash was added to 9 parts by weight, and the water content was 55% by weight.
As a microbial material, the bacterium belonging to the genus Azospirillum and the bacterium belonging to the genus Bradyrizobium obtained in the production examples are blended at a ratio of 10 ⁹ individuals per 1 g of the carrier and uniformly mixed to obtain a microbial material. Got The coal ash used here had a calcium oxide content of 0.5% by weight, an average particle size of 15 μm, and a pH of
Was 10. The coal ash used in the following examples was also the same coal ash.

<Preservation test of each bacterium> The microbial material of each of the above Examples was placed under a temperature condition of 25 ° C., and after 90 days and 240 days, by the same method as the preservation test of the bacteria belonging to the genus Azospirillum. The survival rate was determined by measuring the number of surviving cells. Table 3 shows the survival rates of the bacteria belonging to the genus Azospirillum and the bacteria belonging to the genus Bradyrizobium after 90 days and 240 days in the microbial materials of Examples and Comparative Examples.

[0041]

[Table 3]

From these results, it is understood that the storability of the cells is dramatically improved by adding coal ash to the carrier.

[0043]

Examples 8 to 13 In Example 8, the water content as a carrier was 5
Peat moss adjusted to 0% by weight was used as a carrier in Example 9.
Then add 5 parts by weight of coal ash to 95 parts by weight of peat moss
In Example 10, the content was adjusted to 55% by weight.
Water content by adding 10 parts by weight of coal ash to 90 parts by weight of Tomos
In Example 11 was adjusted to 55% by weight.
Add 70 parts by weight of moss and 30 parts by weight of coal ash to adjust the water content to 5
What was adjusted to 5% by weight was used in Example 12 as peat moss.
Add 50 parts by weight of coal ash to 50 parts by weight to add water content of 55 parts by weight.
What was adjusted to the amount% was peat moss 30 in Example 13.
Add 70 parts by weight of coal ash to 55 parts by weight of water content
Used as a microorganism on each carrier.
Bacteria belonging to the genus Azospirillum obtained in the production example are used as carriers
10 per gram ⁹Mix in proportions of individual and mix evenly
I got biological materials.

<Preservation test of bacteria belonging to the genus Azospirillum> Each of the microbial materials of the above Examples was placed under a temperature condition of 25 ° C., and after 240 days, the same method as the preservation test of bacteria belonging to the genus Azospirillum was performed. The number of surviving cells was measured to determine the survival rate. Table 4 shows the survival rate of the bacteria belonging to the genus Azospirillum after 240 days in the microbial material of each example.

[0045]

[Table 4]

From these results, it was confirmed that the storability of the cells was improved by adding coal ash to the carrier, and the appropriate amount of coal ash was found.

[0047]

Example 14 Peat moss 9 parts by weight of coal ash 1 part by weight was added to the carrier was adjusted to 55 wt% moisture content, bacteria 10 ⁹ individuals per carrier 1g belonging to Azospirillum genus obtained in Production Example Microorganisms, Bacteria belonging to the genus Bradyrizobium were blended at a ratio of 10 ¹⁰ individuals per 1 g of a carrier and uniformly mixed to prepare a microbial material of Example 14.

Crop cultivation was carried out using the microbial material obtained in each of the above examples, and the growth promoting effect of the microbial material of the present invention was evaluated.

<Evaluation of Microbial Material of the Present Invention in Crop Cultivation> (1) Growth Effect on Soybean-1 Soybean varieties (Sapporo Midori) are used as crops, the soil for cultivation is red ball soil, and the container is a 9 cm diameter black vinyl pot. Using. Comparative Example 6 was not added to the cultivation soil, and Comparative Example 7 was used.
In addition, 1 part by weight of coal ash was added to 9 parts by weight of peat moss to adjust the water content to 55% by weight.
In Test Example 2, the microbial material prepared in Example 0 was added to the microbial material prepared in Example 7 at a rate of 1 g per pot, and mixed uniformly. Add soybean seeds to the pot, leaving a 2 cm gap at the top, and add 2 soybean seeds.
Each seed was sown. 20 pots were prepared for each of Comparative Example 6, Comparative Example 7, Test Example 1, and Test Example 2.

Place these pots in a vinyl greenhouse,
Irrigation was performed once a day, and the temperature was controlled to 25 ° C during the day and 15 ° C at night. After the seeds germinated, the seeds were thinned out to one per pot and grown for one month. 1 from the start of cultivation
At the time of a month, all the seedlings were pulled out, the soil attached to the root was washed with water, and the above-ground part and the root part were cut off, and then the stem length was measured. Then, the above-ground part and the root of the sample were separately dried at 110 ° C. for 3 days, and the respective weights were measured. In each Example and Comparative Example, the root weight, ground weight, and stem length were averaged for 20 pieces, and the relative value was calculated with the value of Comparative Example 6 as 100%. Table 5 shows the results.

[0051]

[Table 5]

(2) Growth Effect on Soybean-2 As a crop, a soybean variety (Sapporo Midori) was used, the cultivation soil was red jade soil, and the container was a 9 cm diameter black vinyl pot. In Comparative Example 8, 9 parts by weight of peat moss and 1 part by weight of coal ash were added to the soil for cultivation to adjust the water content to 55% by weight. In Test Example 3, the microbial material produced in Example 10 was used, and in Test Example 4, the microbial material was used. In Test Example 5, the microbial material prepared in Example 7 was added to and uniformly mixed with the microbial material prepared in Example 14 at a rate of 1 g per pot. These cultivation soils were added to the pot leaving a 2 cm gap at the top, and two soybean seeds were sown. 20 pots were prepared for each of Comparative Example 8, Test Example 3, Test Example 4, and Test Example 5.

These pots were managed in the same manner as in the experiment of (1) above, and soybeans were cultivated for one month. afterwards,
In the same manner as the experiment of (1) above, the root weight, the ground weight, and the stem length of soybean were measured for each of the Examples and Comparative Examples, and the average of 20 plants was calculated. Further, the value of Comparative Example 8 was set to 100%. The relative value was calculated. Table 6 shows the results.

[0054]

[Table 6]

From these results, in the crop of the test example in which the microbial material of the present invention was applied to the cultivation soil and cultivated, the crop of the comparative example cultivated without any addition or with only the carrier added. Compared with the above, the weight of the root part, the above-ground part, and the stem length are large, and it can be seen that the growth condition of the whole crop is good. It was also confirmed that it is possible to effectively combine bacteria belonging to the genus Azospirillum with other microorganisms.

[0056]

INDUSTRIAL APPLICABILITY The microbial material for cultivating crops of the present invention is much more stable in terms of storability of microorganisms than conventional microbial materials. Further, when a crop is cultivated using the microbial material of the present invention, the effect of microorganisms can be stably obtained, and efficient cultivation of the crop becomes possible.

Claims (3)

[Claims] 1. A microbial material for crop cultivation containing a carrier containing water and a soil microorganism containing at least a bacterium belonging to the genus Azospirillum, wherein the content of the bacterium belonging to the genus Azospirillum is 2 × 10 ⁸ per 1 g of the carrier. -10
Microbial material for cultivation of ¹² individuals. 2. The carrier is coal ash in an amount of 3 to 7 relative to the total amount of the carrier.
The microbial material according to claim 1, containing 5% by weight. 3. The water content of the carrier is 50 with respect to the total amount of the carrier.
The microbial material according to claim 1 or 2, which is ˜90% by weight.