JPH08511280A - 合成親和性リガンドを用いた免疫グロブリンの精製のための高塩濃度結合バッファー - Google Patents
合成親和性リガンドを用いた免疫グロブリンの精製のための高塩濃度結合バッファーInfo
- Publication number
- JPH08511280A JPH08511280A JP7525628A JP52562895A JPH08511280A JP H08511280 A JPH08511280 A JP H08511280A JP 7525628 A JP7525628 A JP 7525628A JP 52562895 A JP52562895 A JP 52562895A JP H08511280 A JPH08511280 A JP H08511280A
- Authority
- JP
- Japan
- Prior art keywords
- gel
- supernatant
- binding
- igg
- avid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/US1994/003615 WO1995026977A1 (fr) | 1994-04-01 | 1994-04-01 | Tampon de fixation a haute teneur en sel pour la purification d'immunoglobulines avec un ligand d'affinite synthetique |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH08511280A true JPH08511280A (ja) | 1996-11-26 |
Family
ID=22242417
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP7525628A Pending JPH08511280A (ja) | 1994-04-01 | 1994-04-01 | 合成親和性リガンドを用いた免疫グロブリンの精製のための高塩濃度結合バッファー |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0701566A1 (fr) |
JP (1) | JPH08511280A (fr) |
AU (1) | AU7241294A (fr) |
NO (1) | NO954704D0 (fr) |
WO (1) | WO1995026977A1 (fr) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5906747A (en) * | 1995-11-13 | 1999-05-25 | Biosepra Inc. | Separation of molecules from dilute solutions using composite chromatography media having high dynamic sorptive capacity at high flow rates |
ES2797480T3 (es) * | 2005-03-11 | 2020-12-02 | Wyeth Llc | Un procedimiento de cromatografía de reparto débil |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4704366A (en) * | 1984-06-22 | 1987-11-03 | Bio-Rad Laboratories, Inc. | Process for binding IgG to protein A |
-
1994
- 1994-04-01 EP EP94921883A patent/EP0701566A1/fr not_active Withdrawn
- 1994-04-01 WO PCT/US1994/003615 patent/WO1995026977A1/fr not_active Application Discontinuation
- 1994-04-01 AU AU72412/94A patent/AU7241294A/en not_active Abandoned
- 1994-04-01 JP JP7525628A patent/JPH08511280A/ja active Pending
-
1995
- 1995-11-21 NO NO954704A patent/NO954704D0/no unknown
Also Published As
Publication number | Publication date |
---|---|
NO954704L (no) | 1995-11-21 |
EP0701566A1 (fr) | 1996-03-20 |
AU7241294A (en) | 1995-10-23 |
NO954704D0 (no) | 1995-11-21 |
WO1995026977A1 (fr) | 1995-10-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Teng et al. | Affinity chromatography on immobilized “biomimetic” ligands: Synthesis, immobilization and chromatographic assessment of an immunoglobulin G-binding ligand | |
JP5199063B2 (ja) | 弱分配クロマトグラフィー方法 | |
Guerrier et al. | New method for the selective capture of antibodies under physiolgical conditions | |
US10246484B2 (en) | Method for purifying recombinant protein | |
US20100069617A1 (en) | Enhanced protein aggregate removal by mixed mode chromatography on hydrophobic interaction media in the presence of protein-excluded zwitterions | |
Zachariou et al. | Adsorption and selectivity characteristics of several human serum proteins with immobilised hard Lewis metal ion–chelate adsorbents | |
JPH0543719B2 (fr) | ||
Tsang et al. | Optimum dissociating condition for immunoaffinity and preferential isolation of antibodies with high specific activity | |
AU2005217348A1 (en) | Antibody purification | |
Scholz et al. | Salt-independent binding of antibodies from human serum to thiophilic heterocyclic ligands | |
US20180186866A1 (en) | Affinity chromatography purification with low conductivity wash buffer | |
JP2015502959A (ja) | IgG2ジスルフィドアイソフォームの分離 | |
JPH06508058A (ja) | 吸着マトリックス | |
JP6309005B2 (ja) | アルブミンを精製するための方法 | |
US11332514B2 (en) | Cation and anion exchange chromatography method | |
CA1101847A (fr) | Traduction non-disponible | |
JPH08511280A (ja) | 合成親和性リガンドを用いた免疫グロブリンの精製のための高塩濃度結合バッファー | |
Ngo et al. | Avid AL, a synthetic ligand affinity gel mimicking immobilized bacterial antibody receptor for purification of immunoglobulin G | |
US20180186865A1 (en) | Method for the reduction of host cell proteins in affinity chromatography | |
JPWO2021207657A5 (fr) | ||
Ohlson et al. | High-performance liquid affinity chromatographic separation of mouse monoclonal antibodies with protein A silica | |
KR20220101168A (ko) | 이온 교환 크로마토그래피 동안 항체의 수율을 증가시키는 방법 | |
CN100591694C (zh) | 自雁形目鸟类卵中选择性分离IgY抗体的方法及由此获得的IgY抗体 | |
Cochet et al. | Chromatography of human immunoglobulin G on immobilized Drimarene Rubine R/K-5BL Study of mild, efficient elution procedures | |
CA2163619A1 (fr) | Tampon de fixation a haute teneur en sel pour la purification d'immunoglobulines avec un ligand d'affinite synthetique |