JPH08505121A - 分子プローブとしての４，７−ジクロロフルオレセイン染料 - Google Patents

分子プローブとしての４，７−ジクロロフルオレセイン染料

Info

Publication number
JPH08505121A
JPH08505121A JP6507415A JP50741594A JPH08505121A JP H08505121 A JPH08505121 A JP H08505121A JP 6507415 A JP6507415 A JP 6507415A JP 50741594 A JP50741594 A JP 50741594A JP H08505121 A JPH08505121 A JP H08505121A
Authority
JP
Japan
Prior art keywords
dye
dichlorofluorescein
group
dichloro
dyes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
JP6507415A
Other languages
English (en)
Japanese (ja)
Inventor
メンシェン，ステベン，エム
リー，リンダ，ジー
コンネル，チャールス，アール
ハーシー，エヌ，デービス
チャケリアン，バージン
ウー，サム，エル
ステベン フング
Original Assignee
アプライド バイオシステムズ，インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by アプライド バイオシステムズ，インコーポレイテッド filed Critical アプライド バイオシステムズ，インコーポレイテッド
Publication of JPH08505121A publication Critical patent/JPH08505121A/ja
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/78—Ring systems having three or more relevant rings
    • C07D311/80—Dibenzopyrans; Hydrogenated dibenzopyrans
    • C07D311/82—Xanthenes
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/06—Hydroxy derivatives of triarylmethanes in which at least one OH group is bound to an aryl nucleus and their ethers or esters
    • C09B11/08—Phthaleins; Phenolphthaleins; Fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pyrane Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Saccharide Compounds (AREA)
JP6507415A 1992-09-03 1993-09-01 分子プローブとしての４，７−ジクロロフルオレセイン染料 Withdrawn JPH08505121A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US93981392A 1992-09-03 1992-09-03
US939,813 1992-09-03
PCT/US1993/008227 WO1994005688A1 (en) 1992-09-03 1993-09-01 4,7-dichlorofluorescein dyes as molecular probes

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2004024814A Division JP4554224B2 (ja) 1992-09-03 2004-01-30 分子プローブとしての４，７−ジクロロフルオレセイン染料

Publications (1)

Publication Number Publication Date
JPH08505121A true JPH08505121A (ja) 1996-06-04

Family

ID=25473780

Family Applications (4)

Application Number Title Priority Date Filing Date
JP6507415A Withdrawn JPH08505121A (ja) 1992-09-03 1993-09-01 分子プローブとしての４，７−ジクロロフルオレセイン染料
JP2004024814A Active JP4554224B2 (ja) 1992-09-03 2004-01-30 分子プローブとしての４，７−ジクロロフルオレセイン染料
JP2008177253A Withdrawn JP2009000119A (ja) 1992-09-03 2008-07-07 分子プローブとしての４，７−ジクロロフルオレセイン染料
JP2011054840A Withdrawn JP2011162548A (ja) 1992-09-03 2011-03-11 分子プローブとしての４，７−ジクロロフルオレセイン染料

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2004024814A Active JP4554224B2 (ja) 1992-09-03 2004-01-30 分子プローブとしての４，７−ジクロロフルオレセイン染料
JP2008177253A Withdrawn JP2009000119A (ja) 1992-09-03 2008-07-07 分子プローブとしての４，７−ジクロロフルオレセイン染料
JP2011054840A Withdrawn JP2011162548A (ja) 1992-09-03 2011-03-11 分子プローブとしての４，７−ジクロロフルオレセイン染料

Country Status (4)

Country Link
EP (1) EP0658167B1 (US07906523-20110315-C00004.png)
JP (4) JPH08505121A (US07906523-20110315-C00004.png)
DE (1) DE69324494T2 (US07906523-20110315-C00004.png)
WO (1) WO1994005688A1 (US07906523-20110315-C00004.png)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001508494A (ja) * 1997-09-23 2001-06-26 モレキュラー プローブス インコーポレーテッド スルホン化キサンテン誘導体
JP2004043819A (ja) * 1996-05-03 2004-02-12 Applera Corp 硬質リンカ―を有するエネルギ―転移色素
JP2004225049A (ja) * 1992-09-03 2004-08-12 Applera Corp 分子プローブとしての４，７−ジクロロフルオレセイン染料

Families Citing this family (39)

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US5658751A (en) * 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US6020481A (en) * 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US6162931A (en) 1996-04-12 2000-12-19 Molecular Probes, Inc. Fluorinated xanthene derivatives
AU2678997A (en) * 1996-04-19 1997-11-12 Lexigen Pharmaceuticals Corp. Inhibition of the binding of human ige to its receptor by tetracyclic compounds for the alleviation of ige-mediated immune response
US7825237B2 (en) 1996-05-03 2010-11-02 Applied Biosystems, Llc Oligonucleotides and analogs labeled with energy transfer dyes
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US7388092B2 (en) 1996-05-03 2008-06-17 Applera Corporation Oligonucleotides and analogs labeled with energy transfer dyes
US5847162A (en) * 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
US6080852A (en) 1996-06-27 2000-06-27 The Perkin-Elmer Corporation 4,7-dichlororhodamine dyes
US6017712A (en) * 1996-06-27 2000-01-25 Lee; Linda 4,7-dichlororhodamine dyes
US5696157A (en) * 1996-11-15 1997-12-09 Molecular Probes, Inc. Sulfonated derivatives of 7-aminocoumarin
US5830912A (en) * 1996-11-15 1998-11-03 Molecular Probes, Inc. Derivatives of 6,8-difluoro-7-hydroxycoumarin
US6008379A (en) * 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US5936087A (en) * 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
US6583168B1 (en) 1997-11-25 2003-06-24 Applera Corporation Sulfonated diarylrhodamine dyes
US6664047B1 (en) 1999-04-30 2003-12-16 Molecular Probes, Inc. Aza-benzazolium containing cyanine dyes
JP2003507466A (ja) 1999-08-19 2003-02-25 ジェンテスト・コーポレーション ハイスループットチトクロームｐ４５０阻害アッセイにおけるフルオレセインアリールエーテル類の使用
US6221604B1 (en) * 2000-02-07 2001-04-24 Pe Corporation Electron-deficient nitrogen heterocycle-substituted fluorescein dyes
US7169922B2 (en) 2000-08-04 2007-01-30 Invitrogen Corporation Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings
AU2001279185B2 (en) 2000-08-04 2005-07-07 Molecular Probes, Inc. Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings
EP1322710B2 (en) 2000-09-29 2015-02-18 Life Technologies Corporation Modified carbocyanine dyes and their conjugates
DE10106682A1 (de) 2001-02-14 2002-08-29 Clariant Gmbh Farbmittelkomposition
US7005518B2 (en) 2002-10-25 2006-02-28 Li-Cor, Inc. Phthalocyanine dyes
US7417726B2 (en) 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
EP1685117B1 (en) 2003-10-31 2015-01-07 Life Technologies Corporation Fluorinated resorufin compounds and their application in detecting hydrogen peroxide
US20050214807A1 (en) 2003-11-19 2005-09-29 Iain Johnson Environmental sensitive fluorogenic compounds and their application for singlet oxygen and protein detection
US7776529B2 (en) 2003-12-05 2010-08-17 Life Technologies Corporation Methine-substituted cyanine dye compounds
US8039642B2 (en) 2003-12-09 2011-10-18 Life Technologies Corporation Pyrenyloxysulfonic acid fluorescent agents
US7598390B2 (en) 2005-05-11 2009-10-06 Life Technologies Corporation Fluorescent chemical compounds having high selectivity for double stranded DNA, and methods for their use
US7888506B2 (en) 2007-01-26 2011-02-15 Duquesne University Of The Holy Spirit Composition, synthesis, and use of a new class of fluorophores
US8586743B2 (en) 2007-01-30 2013-11-19 Life Technologies Corporation Labeling reagents and methods of their use
EP2482076A1 (en) 2007-07-31 2012-08-01 Osaka University Composition for measuring the binding affinity between nucleic acid and test substance, and use thereof
JP5730311B2 (ja) 2009-08-31 2015-06-10 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 反応性シアニン化合物
US8623324B2 (en) 2010-07-21 2014-01-07 Aat Bioquest Inc. Luminescent dyes with a water-soluble intramolecular bridge and their biological conjugates
EP2782916B1 (en) 2011-11-21 2018-02-28 Promega Corporation Carboxy x rhodamine analogs
US9139868B2 (en) 2013-03-15 2015-09-22 Promega Corporation Silicon and germanium dyes for use in genetic identity
WO2015175870A1 (en) 2014-05-16 2015-11-19 Life Technologies Corporation Carbopyronone compounds useful as diagnostic adjuvants
WO2017172608A1 (en) 2016-03-28 2017-10-05 Aat Bioquest, Inc. Polyfluoreno[4,5-cde]oxepine conjugates and their use in methods of analyte detection
CN115280147A (zh) 2020-01-30 2022-11-01 阿特生物探索公司 可紫外激发的基于聚芴的缀合物及其在分析物检测方法中的用途

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4230558A (en) 1978-10-02 1980-10-28 Coulter Electronics, Inc. Single drop separator
US4318846A (en) * 1979-09-07 1982-03-09 Syva Company Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers
JPS5782387A (en) * 1980-10-29 1982-05-22 Syva Co Novel ether substituted fluorescein compound
US4439356A (en) 1981-03-03 1984-03-27 Syva Company Unsymmetrical fluorescein derivatives
JPS61500202A (ja) 1983-08-31 1986-02-06 ルメックス、インコーポレイテッド 減衰型流体変位支持装置およびその製法
US5188934A (en) * 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
DE69324494T2 (de) * 1992-09-03 1999-09-23 Perkin Elmer Corp 4,7-dichlorofluoroszein-farbestoffe als molekulare sonden

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004225049A (ja) * 1992-09-03 2004-08-12 Applera Corp 分子プローブとしての４，７−ジクロロフルオレセイン染料
JP4554224B2 (ja) * 1992-09-03 2010-09-29 アプライド バイオシステムズ， エルエルシー 分子プローブとしての４，７−ジクロロフルオレセイン染料
JP2004043819A (ja) * 1996-05-03 2004-02-12 Applera Corp 硬質リンカ―を有するエネルギ―転移色素
JP2001508494A (ja) * 1997-09-23 2001-06-26 モレキュラー プローブス インコーポレーテッド スルホン化キサンテン誘導体

Also Published As

Publication number Publication date
JP2009000119A (ja) 2009-01-08
DE69324494T2 (de) 1999-09-23
WO1994005688A1 (en) 1994-03-17
JP2011162548A (ja) 2011-08-25
EP0658167B1 (en) 1999-04-14
DE69324494D1 (de) 1999-05-20
EP0658167A1 (en) 1995-06-21
JP2004225049A (ja) 2004-08-12
JP4554224B2 (ja) 2010-09-29

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Legal Events

Date Code Title Description
A521 Request for written amendment filed

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Effective date: 20040130

A911 Transfer to examiner for re-examination before appeal (zenchi)

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Effective date: 20040601

A912 Re-examination (zenchi) completed and case transferred to appeal board

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Effective date: 20040617

A761 Written withdrawal of application

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Effective date: 20080423