DE69324494D1 - 4,7-dichlorofluoroszein-farbestoffe als molekulare sonden - Google Patents

4,7-dichlorofluoroszein-farbestoffe als molekulare sonden

Info

Publication number
DE69324494D1
DE69324494D1 DE69324494T DE69324494T DE69324494D1 DE 69324494 D1 DE69324494 D1 DE 69324494D1 DE 69324494 T DE69324494 T DE 69324494T DE 69324494 T DE69324494 T DE 69324494T DE 69324494 D1 DE69324494 D1 DE 69324494D1
Authority
DE
Germany
Prior art keywords
dichlorofluoroszein
dyes
molecular probe
probe
molecular
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69324494T
Other languages
English (en)
Other versions
DE69324494T2 (de
Inventor
Steven Menchen
Linda Lee
Charles Connell
N Hershey
Vergine Chakerian
Sam Woo
Steven Fung
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of DE69324494D1 publication Critical patent/DE69324494D1/de
Publication of DE69324494T2 publication Critical patent/DE69324494T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/78—Ring systems having three or more relevant rings
    • C07D311/80—Dibenzopyrans; Hydrogenated dibenzopyrans
    • C07D311/82—Xanthenes
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/06—Hydroxy derivatives of triarylmethanes in which at least one OH group is bound to an aryl nucleus and their ethers or esters
    • C09B11/08—Phthaleins; Phenolphthaleins; Fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pyrane Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Saccharide Compounds (AREA)
DE69324494T 1992-09-03 1993-09-01 4,7-dichlorofluoroszein-farbestoffe als molekulare sonden Expired - Lifetime DE69324494T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US93981392A 1992-09-03 1992-09-03
PCT/US1993/008227 WO1994005688A1 (en) 1992-09-03 1993-09-01 4,7-dichlorofluorescein dyes as molecular probes

Publications (2)

Publication Number Publication Date
DE69324494D1 true DE69324494D1 (de) 1999-05-20
DE69324494T2 DE69324494T2 (de) 1999-09-23

Family

ID=25473780

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69324494T Expired - Lifetime DE69324494T2 (de) 1992-09-03 1993-09-01 4,7-dichlorofluoroszein-farbestoffe als molekulare sonden

Country Status (4)

Country Link
EP (1) EP0658167B1 (de)
JP (4) JPH08505121A (de)
DE (1) DE69324494T2 (de)
WO (1) WO1994005688A1 (de)

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JPH08505121A (ja) * 1992-09-03 1996-06-04 アプライド バイオシステムズ，インコーポレイテッド 分子プローブとしての４，７−ジクロロフルオレセイン染料
US5658751A (en) * 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US6020481A (en) 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US6162931A (en) * 1996-04-12 2000-12-19 Molecular Probes, Inc. Fluorinated xanthene derivatives
WO1997040033A1 (en) * 1996-04-19 1997-10-30 Lexigen Pharmaceuticals Corp. Inhibition of the binding of human ige to its receptor by tetracyclic compounds for the alleviation of ige-mediated immune response
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5847162A (en) * 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
US7825237B2 (en) 1996-05-03 2010-11-02 Applied Biosystems, Llc Oligonucleotides and analogs labeled with energy transfer dyes
US7388092B2 (en) 1996-05-03 2008-06-17 Applera Corporation Oligonucleotides and analogs labeled with energy transfer dyes
US6080852A (en) 1996-06-27 2000-06-27 The Perkin-Elmer Corporation 4,7-dichlororhodamine dyes
US6017712A (en) * 1996-06-27 2000-01-25 Lee; Linda 4,7-dichlororhodamine dyes
US5830912A (en) * 1996-11-15 1998-11-03 Molecular Probes, Inc. Derivatives of 6,8-difluoro-7-hydroxycoumarin
US5696157A (en) * 1996-11-15 1997-12-09 Molecular Probes, Inc. Sulfonated derivatives of 7-aminocoumarin
US6130101A (en) * 1997-09-23 2000-10-10 Molecular Probes, Inc. Sulfonated xanthene derivatives
US6008379A (en) * 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US6583168B1 (en) 1997-11-25 2003-06-24 Applera Corporation Sulfonated diarylrhodamine dyes
US5936087A (en) * 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
US6664047B1 (en) 1999-04-30 2003-12-16 Molecular Probes, Inc. Aza-benzazolium containing cyanine dyes
US6420131B1 (en) 1999-08-19 2002-07-16 Gentest Corporation Use of fluorescein aryl ethers in high throughput cytochrome P450 inhibition assays
US6221604B1 (en) 2000-02-07 2001-04-24 Pe Corporation Electron-deficient nitrogen heterocycle-substituted fluorescein dyes
US7169922B2 (en) 2000-08-04 2007-01-30 Invitrogen Corporation Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings
US6716979B2 (en) 2000-08-04 2004-04-06 Molecular Probes, Inc. Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings
ATE352586T2 (de) 2000-09-29 2007-02-15 Molecular Probes Inc Modifizierte carbocyaninfarbstoffe und deren konjugate
DE10106682A1 (de) 2001-02-14 2002-08-29 Clariant Gmbh Farbmittelkomposition
US7005518B2 (en) 2002-10-25 2006-02-28 Li-Cor, Inc. Phthalocyanine dyes
US7417726B2 (en) 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
US7432372B2 (en) 2003-10-31 2008-10-07 Invitrogen Corporation Fluorinated resorufin compounds and their application
US20050214807A1 (en) 2003-11-19 2005-09-29 Iain Johnson Environmental sensitive fluorogenic compounds and their application for singlet oxygen and protein detection
US7776529B2 (en) 2003-12-05 2010-08-17 Life Technologies Corporation Methine-substituted cyanine dye compounds
US8039642B2 (en) 2003-12-09 2011-10-18 Life Technologies Corporation Pyrenyloxysulfonic acid fluorescent agents
JP5306811B2 (ja) 2005-05-11 2013-10-02 ライフ テクノロジーズ コーポレーション ２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用
US7888506B2 (en) 2007-01-26 2011-02-15 Duquesne University Of The Holy Spirit Composition, synthesis, and use of a new class of fluorophores
US8586743B2 (en) 2007-01-30 2013-11-19 Life Technologies Corporation Labeling reagents and methods of their use
EP2175275A4 (de) 2007-07-31 2010-09-01 Univ Osaka Zusammensetzung zur messung der bindungsaffinität zwischen nukleinsäure und substanz sowie ihre verwendung
EP2473563B1 (de) 2009-08-31 2019-06-19 Promega Corporation Reaktive cyaninverbindungen
US8623324B2 (en) 2010-07-21 2014-01-07 Aat Bioquest Inc. Luminescent dyes with a water-soluble intramolecular bridge and their biological conjugates
WO2013078244A1 (en) 2011-11-21 2013-05-30 Promega Corporation Carboxy x rhodamine analogs
JP6367307B2 (ja) 2013-03-15 2018-08-01 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 遺伝的同一性における使用のための新規ケイ素およびゲルマニウム色素
WO2015175870A1 (en) 2014-05-16 2015-11-19 Life Technologies Corporation Carbopyronone compounds useful as diagnostic adjuvants
CN108473505B (zh) 2016-03-28 2019-11-05 阿特生物探索公司 聚芴并[4,5-cde]氧杂环庚三烯偶联物及其在分析物检测方法中的用途
WO2021154933A1 (en) 2020-01-30 2021-08-05 Aat Bioquest, Inc. Uv excitable polyfluorene based conjugates and their use in methods of analyte detection

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Publication number Priority date Publication date Assignee Title
US4230558A (en) 1978-10-02 1980-10-28 Coulter Electronics, Inc. Single drop separator
US4318846A (en) * 1979-09-07 1982-03-09 Syva Company Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers
JPS5782387A (en) * 1980-10-29 1982-05-22 Syva Co Novel ether substituted fluorescein compound
US4439356A (en) 1981-03-03 1984-03-27 Syva Company Unsymmetrical fluorescein derivatives
DE3382290D1 (de) 1983-08-31 1991-06-20 Lumex Traegersystem zur verlagerung von daempferfluessigkeit und methode zu dessen herstellung.
US5188934A (en) * 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
JPH08505121A (ja) * 1992-09-03 1996-06-04 アプライド バイオシステムズ，インコーポレイテッド 分子プローブとしての４，７−ジクロロフルオレセイン染料

Also Published As

Publication number Publication date
JP2011162548A (ja) 2011-08-25
EP0658167A1 (de) 1995-06-21
JP2009000119A (ja) 2009-01-08
JP4554224B2 (ja) 2010-09-29
JP2004225049A (ja) 2004-08-12
EP0658167B1 (de) 1999-04-14
DE69324494T2 (de) 1999-09-23
JPH08505121A (ja) 1996-06-04
WO1994005688A1 (en) 1994-03-17

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: APPLERA CORP., FOSTER CITY, CALIF., US

8327 Change in the person/name/address of the patent owner

Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US

8380 Miscellaneous part iii

Free format text: PFANDRECHT

8380 Miscellaneous part iii

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