JPH08266255A - Composition for improving learning ability - Google Patents
Composition for improving learning abilityInfo
- Publication number
- JPH08266255A JPH08266255A JP7099779A JP9977995A JPH08266255A JP H08266255 A JPH08266255 A JP H08266255A JP 7099779 A JP7099779 A JP 7099779A JP 9977995 A JP9977995 A JP 9977995A JP H08266255 A JPH08266255 A JP H08266255A
- Authority
- JP
- Japan
- Prior art keywords
- sialic acid
- learning ability
- containing oligosaccharide
- oligosaccharide derivative
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Seeds, Soups, And Other Foods (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、シアル酸類を含有する
オリゴ糖誘導体が学習能力を向上する効果を見いだし、
その機能を食品及び医薬品へ応用することを目的とする
学習能力向上組成物に関する。INDUSTRIAL APPLICABILITY The present invention has found that oligosaccharide derivatives containing sialic acids improve learning ability,
The present invention relates to a learning ability improving composition intended to apply its function to foods and pharmaceuticals.
【０００２】[0002]
【従来の技術】近年、学習能力や記憶等の脳の機能を高
める物質の探索が盛んに行われている。学習能力を向上
させる成分としてドコサヘキサエン酸(DHA) が広く用い
られており、特開平１−２７９８２７公報記載の例で
は、ドコサヘキサエン酸はラットのＹ字迷路明暗弁別餌
取り実験で正反応率（％）を向上させることが知られて
いた。しかしながら、ドコサヘキサエン酸を食品等に添
加する際、独特の魚臭があり、その使用についてはいく
つかの限定が余儀なくされている現状であった。食品や
医薬品に用いる際に臭いや味に影響せず、学習能力や記
憶等の脳の機能を高める物質の開発と安定供給が強く求
められている。従来ガングリオシドは、脳細胞中に多く
発見されており脳の構成成分であり、学習能力や記憶等
の脳の機能を高める物質である可能性を持っていること
が知られている。たとえば、生後間もない段階で刺激を
与えたラットは、学習能力の点でコントロール群と比べ
優れており、脳内のシアル酸の含量を定量したところ有
意に増大することが報告されている（B. L. G. Morgan
and M. Winick: J. Nutr., 110, 425-432 (1980)）。し
かしながら、シアル酸含有オリゴ糖誘導体について、こ
の様な作用があることは知られていなかった。また、シ
アル酸含有オリゴ糖誘導体を工業的に調製する方法は知
られておらず、それらを産業上有効に利用する手段が構
成されていなかった。2. Description of the Related Art In recent years, substances that enhance brain functions such as learning ability and memory have been actively searched for. Docosahexaenoic acid (DHA) is widely used as a component for improving learning ability. In the example described in JP-A-1-279827, docosahexaenoic acid is a positive reaction rate (%) in a rat Y-shaped maze light / dark discrimination feeding experiment. Was known to improve. However, when docosahexaenoic acid is added to foods or the like, there is a peculiar fishy odor, and there are some restrictions on its use. There is a strong demand for the development and stable supply of substances that enhance brain functions such as learning ability and memory without affecting odor and taste when used in foods and pharmaceuticals. Many gangliosides have been conventionally found in brain cells, and are known to be constituent components of the brain and have the potential to be substances that enhance brain functions such as learning ability and memory. For example, it was reported that the rats stimulated in the early postnatal period were superior to the control group in terms of learning ability, and that the amount of sialic acid in the brain was quantified to be significantly increased ( BLG Morgan
and M. Winick: J. Nutr., 110, 425-432 (1980)). However, it has not been known that the sialic acid-containing oligosaccharide derivative has such an action. Further, a method for industrially preparing a sialic acid-containing oligosaccharide derivative has not been known, and a means for effectively utilizing them has not been established.
【０００３】[0003]
【発明が解決しようとする課題】本発明は、シアル酸含
有オリゴ糖誘導体を有効成分として含有することを特徴
とする学習能力向上組成物を提供するものである。DISCLOSURE OF THE INVENTION The present invention provides a composition for improving learning ability, which comprises a sialic acid-containing oligosaccharide derivative as an active ingredient.
【０００４】[0004]
【課題を解決するための手段】本発明者らは、動物実験
の結果、シアル酸含有オリゴ糖誘導体が学習能力や記憶
等の脳の機能を高める物質であることを見いだし、本発
明を完成するにいたった。本願におけるシアル酸含有オ
リゴ糖誘導体とは、糖タンパク質分子又はガングリオシ
ドより遊離して存在するオリゴ糖及びオリゴ糖ペプチド
に限定されるものではなく、糖タンパク質分子中に存在
するオリゴ糖鎖、ガングリオシド中に存在するオリゴ糖
鎖をもさす。特に限定されるものではないが、好ましく
は、ペプチド分子と脂質分子を含まないシアル酸含有オ
リゴ糖誘導体と糖タンパク質分子由来のシアル酸含有オ
リゴ糖誘導体及びシアル酸含有オリゴ糖ペプチド、さら
に好ましくは糖タンパク質分子に結合したシアル酸含有
オリゴ糖誘導体及びシアル酸含有オリゴ糖ペプチドであ
る。As a result of animal experiments, the present inventors have found that a sialic acid-containing oligosaccharide derivative is a substance that enhances brain functions such as learning ability and memory, and completes the present invention. Came to The sialic acid-containing oligosaccharide derivative in the present application is not limited to oligosaccharides and oligoglycopeptides that are present free from glycoprotein molecules or gangliosides, and oligosaccharide chains and gangliosides present in glycoprotein molecules are not limited. It also refers to the oligosaccharide chains present. Although not particularly limited, preferably, a sialic acid-containing oligosaccharide derivative containing no peptide molecule and lipid molecule, a sialic acid-containing oligosaccharide derivative derived from a glycoprotein molecule, and a sialic acid-containing oligosaccharide peptide, and more preferably a sugar A sialic acid-containing oligosaccharide derivative and a sialic acid-containing oligosaccharide peptide bound to a protein molecule.
【０００５】本発明におけるシアル酸含有オリゴ糖誘導
体は、シアル酸が１残基以上オリゴ糖鎖分子に共有結合
し、かつアミノ酸を含まない低分子性の遊離糖鎖、また
はシアル酸類が１残基以上オリゴ糖ペプチド分子又は脂
質に共有結合している低分子性化合物である。ここでい
う糖ペプチドとは、オリゴ糖鎖にアミノ酸が１残基以上
結合した化合物をさす。ここでいうシアル酸とは、ノイ
ラミン酸のアシル誘導体であり、天然には、３０種類以
上の誘導体が存在する。特に限定するものではないがシ
アル酸の代表的なものは N−アセチルノイラミン酸やＮ
−グリコリルノイラミン酸である。The sialic acid-containing oligosaccharide derivative according to the present invention is a low-molecular free sugar chain in which sialic acid has one or more residues covalently bonded to an oligosaccharide chain molecule and contains no amino acid, or sialic acids have one residue. These are low molecular weight compounds that are covalently bound to oligosaccharide peptide molecules or lipids. The term “glycopeptide” as used herein refers to a compound in which one or more amino acid residues are bound to an oligosaccharide chain. The sialic acid here is an acyl derivative of neuraminic acid, and naturally there are 30 or more kinds of derivatives. Although not particularly limited, typical examples of sialic acid include N-acetylneuraminic acid and N
-Glycolyl neuraminic acid.
【０００６】本発明におけるシアル酸含有オリゴ糖誘導
体の起源については特に限定するものではないが、好ま
しくは鶏卵卵黄が望ましい。本発明における鶏卵卵黄と
は、ニワトリの卵の生卵黄、またはその加工品である液
状卵黄、卵黄粉末、凍結卵黄、脱脂処理卵黄などをさ
す。シアル酸含有オリゴ糖誘導体の製造法としては、特
に限定するものではないが、鶏卵卵黄に水または塩溶液
を加え、４〜８０℃、好ましくは４〜４０℃で１０分〜
３日攪拌した後、濾過を行い、得られた抽出濾液を脱塩
・濃縮し、さらに乾燥することによりシアル酸含有オリ
ゴ糖誘導体を得る方法がよい。上記記載の水又は塩溶液
とは糖タンパク質を効率良く抽出するために最適なイオ
ン強度を得るためのものであり、塩としてはナトリウム
塩及びカリウム塩など、例えばNaCl、KCl などの水溶性
の高い塩、Tris-HCl、AcOH-AcONaなどの緩衝液などであ
れば良い。また、塩濃度は塩が溶け得る量であれば特に
限定されるものではないが、好ましくは０〜０．５Ｍの
濃度が抽出効率の点で良い。The origin of the sialic acid-containing oligosaccharide derivative in the present invention is not particularly limited, but chicken egg yolk is preferable. The chicken egg yolk in the present invention refers to a raw egg yolk of a chicken egg, or a processed egg yolk such as liquid egg yolk, egg yolk powder, frozen egg yolk, and defatted egg yolk. The method for producing the sialic acid-containing oligosaccharide derivative is not particularly limited, but water or salt solution is added to chicken egg yolk at 4 to 80 ° C, preferably 4 to 40 ° C for 10 minutes to
After stirring for 3 days, filtration is performed, and the obtained extraction filtrate is desalted / concentrated, and further dried to obtain a sialic acid-containing oligosaccharide derivative. The water or salt solution described above is for obtaining the optimum ionic strength for efficient extraction of glycoprotein, and as the salt, sodium salt and potassium salt, etc., for example, highly soluble in water such as NaCl and KCl. Any buffer such as salt, Tris-HCl, AcOH-AcONa, etc. may be used. The salt concentration is not particularly limited as long as the salt can be dissolved, but a concentration of 0 to 0.5 M is preferable in terms of extraction efficiency.
【０００７】本発明のシアル酸含有オリゴ糖誘導体は、
抽出物をそのまま用いるだけではなく、より好ましくは
これらの酵素処理物を用いることが、より活性の強い成
分を得られることから望ましい。鶏卵卵黄に水または塩
溶液を加えた懸濁液、または鶏卵卵黄の水または塩溶液
による抽出液を酵素消化することにより卵黄中の高分子
性物質を水溶化させる。用いられる酵素としては糖分解
酵素、蛋白分解酵素、脂質分解酵素が挙げられる。蛋白
分解酵素は、鶏卵卵黄中の糖タンパク質からシアル酸含
有オリゴ糖ペプチドを遊離させ、脂質分解酵素は、ガン
グリオシドのようなシアル酸含有糖脂質からシアル酸含
有オリゴ糖誘導体を遊離させる。また、糖分解酵素は、
糖タンパク質や糖脂質からシアル酸含有オリゴ糖誘導体
を切り出してくる。これら酵素は特に限定されるもので
はなく上記記載の活性を有する酵素であればいずれを用
いてもかまわない。例えばAspergillus 属、Bacillus属
などの産生するプロテアーゼ等が考えられ、すなわち具
体的にはオリエンターゼ ONS、90N(阪急バイオ社製）、
プロテアーゼＮ（天野製薬社製）、ＡＯプロテアーゼ
（盛進製薬社製）、スミチームＬＰ−２０（新日本化学
社製）などの食品添加物用プロテアーゼ製剤や、アクチ
ナーゼＥ、キモトリプシン、トリプシン、サーモリシン
などの酵素製剤などを用いることができる。本発明のシ
アル酸含有オリゴ糖誘導体を有効成分とする学習能力向
上組成物は、医薬品や食品への用途に用いられ、形態と
しては粉末状のもの溶液状のものペレット状のものいず
れのものでも使用される。これらは、いずれの形状でも
使用され製剤の形態に合わせてもっとも用いやすい形態
で使用される。製剤としてその添加量は特に限定される
ものではないが０．１〜２０％の割合で用いられる。ま
たさらに望ましくは、０．１〜１０％の割合で添加して
使用する。The sialic acid-containing oligosaccharide derivative of the present invention is
It is desirable not only to use the extract as it is, but more preferably to use these enzyme-treated products because a component having higher activity can be obtained. A polymeric substance in egg yolk is solubilized by enzymatically digesting a suspension of chicken egg yolk with water or a salt solution or an extract of chicken egg yolk with a water or salt solution. Examples of the enzyme used include sugar degrading enzyme, proteolytic enzyme, and lipolytic enzyme. Proteolytic enzymes release sialic acid-containing oligosaccharide peptides from glycoproteins in egg yolk, and lipolytic enzymes release sialic acid-containing oligosaccharide derivatives from sialic acid-containing glycolipids such as gangliosides. In addition, the glycolytic enzyme is
Sialic acid-containing oligosaccharide derivatives are extracted from glycoproteins and glycolipids. These enzymes are not particularly limited, and any enzyme may be used as long as it has the above-mentioned activity. For example, Aspergillus genus, proteases produced by Bacillus genus, etc. are considered, that is, specifically, orientase ONS, 90N (manufactured by Hankyu Bio Inc.),
Protease formulations for food additives such as Protease N (manufactured by Amano Pharmaceutical Co., Ltd.), AO protease (manufactured by Morishin Pharmaceutical Co., Ltd.), Sumiteam LP-20 (manufactured by Shin Nippon Kagaku Co., Ltd.), actinase E, chymotrypsin, trypsin, thermolysin, etc. An enzyme preparation or the like can be used. The learning ability-improving composition containing the sialic acid-containing oligosaccharide derivative of the present invention as an active ingredient is used for application to medicines and foods, and the form is powdery or solution-like pellets used. These are used in any shape and are used in the most convenient form according to the form of the preparation. The amount of addition as a formulation is not particularly limited, but it is used at a rate of 0.1 to 20%. Further, more preferably, it is used by adding at a rate of 0.1 to 10%.
【０００８】[0008]
実施例１ 鶏卵由来脱脂卵黄 100ｋｇを 1 m3 の 0.5 M NaCl 溶液
に懸濁し、室温で３時間攪拌した。濾紙（No．２；Adva
ntec）により濾過した後、４℃で２日静置し得られた上
清に分画分子量２万の膜を用い限外濾過を施した。濾過
液をさらに逆浸透膜（RO膜）により脱塩し、スプレード
ライにより乾燥したところ 134ｇの本発明品である学習
能力組成物を得た。本発明品である学習能力組成物のシ
アル酸含量についてTBA 発色法に基づき測定したところ
遊離のシアル酸は認められなかった。次に本発明品であ
る学習能力組成物（シアル酸含有オリゴ糖誘導体）を
０．１Ｎ Ｈ2 ＳＯ4 を用い８０℃，１時間加水分解し
た後にシアル酸含量を TBA発色法により定量したところ
1.5％ (w/w)と算出された。 実施例２ 脱脂卵黄４０ｋｇに水 160Ｌ、オリエンターゼ ONS 2.0
ｋｇを加え５０℃、１５時間インキュベート後濾過し、
得られた瀘液を分画分子量１万以下の膜を用いて限外濾
過した。濾過液を逆浸透膜により脱塩し、スプレードラ
イにより乾燥したところ、520 ｇの本発明品である学習
能力組成物を得た。本発明品である学習能力組成物のシ
アル酸含有についてTBA 発色法に基づき測定したところ
遊離のシアル酸は認められなかった。次に本発明品であ
る学習能力組成物（シアル酸含有オリゴ糖誘導体）を
０．１Ｎ Ｈ2 ＳＯ4 を用い８０℃，１時間加水分解し
た後にシアル酸含量を定量したところ TBA発色法により
１１％ (w/w)と算出された。Example 1 100 kg of defatted egg yolk derived from chicken egg was suspended in 1 m 3 of 0.5 M NaCl solution and stirred at room temperature for 3 hours. Filter paper (No.2; Adva
The resulting supernatant was subjected to ultrafiltration using a membrane having a molecular weight cut off of 20,000. The filtrate was further desalted with a reverse osmosis membrane (RO membrane) and dried by spray drying to obtain 134 g of the learning ability composition of the present invention. When the sialic acid content of the learning ability composition of the present invention was measured by the TBA color development method, no free sialic acid was observed. Next, the learning ability composition of the present invention (sialic acid-containing oligosaccharide derivative) was hydrolyzed with 0.1N H 2 SO 4 at 80 ° C. for 1 hour, and then the sialic acid content was quantified by the TBA coloring method.
It was calculated to be 1.5% (w / w). Example 2 40 kg of defatted egg yolk, 160 L of water, orientase ONS 2.0
Add kg and incubate at 50 ° C for 15 hours, then filter,
The obtained filtrate was ultrafiltered using a membrane having a molecular weight cutoff of 10,000 or less. The filtrate was desalted with a reverse osmosis membrane and dried by spray drying to obtain 520 g of the learning ability composition of the present invention. When the content of sialic acid in the learning ability composition of the present invention was measured by the TBA color development method, no free sialic acid was found. Next, the learning ability composition (sialic acid-containing oligosaccharide derivative) which is the product of the present invention was hydrolyzed with 0.1N H 2 SO 4 at 80 ° C. for 1 hour and then the sialic acid content was quantified. % (W / w) was calculated.
【０００９】試験例１ 実施例１で得られた本発明品である学習能力組成物（シ
アル酸含有オリゴ糖誘導体）の学習能力向上効果を調べ
るため乳児ラットを用いて実験を行った。まず、７日令
のＳＤラットにサンプルを飼料中に規定量添加する。ラ
ットへのサンプルの投与は、２週間のみ行い、その後
は、通常の飼料を与えた。試験区として本発明品である
学習能力組成物（シアル酸含有オリゴ糖誘導体）０．５
％，DHA ０．５％，生理食塩水の３種で実験を行った。
２１日令から１週間、毎日図１に示す迷路を走らせスタ
ートからゴールまでの到達時間の平均とゴールまでの到
達の割合から各サンプルの学習能力向上効果を測定し
た。結果を図２，図３に示す。図２，図３より明らかな
ようにシアル酸含有オリゴ糖誘導体は、DHA 以上にゴー
ルまでの到達時間もその到達率も優れており、乳児期で
のシアル酸含有オリゴ糖誘導体の添加は脳の発達に関与
し、結果的に学習能力の獲得に優れた効果を発揮した。Test Example 1 Experiments were carried out using infant rats in order to examine the effect of improving the learning ability of the learning ability composition (sialic acid-containing oligosaccharide derivative) which is the product of the present invention obtained in Example 1. First, a specified amount of a sample is added to the diet of 7-day-old SD rats. The samples were administered to the rats for only 2 weeks, after which they were fed a normal diet. A learning ability composition (sialic acid-containing oligosaccharide derivative) which is the product of the present invention as a test section 0.5
%, DHA 0.5%, and three kinds of physiological saline.
The maze shown in FIG. 1 was run daily for one week from the 21st day of age, and the learning ability improving effect of each sample was measured from the average time to reach the goal from the start and the rate of reaching the goal. The results are shown in FIGS. As is clear from Fig. 2 and Fig. 3, the sialic acid-containing oligosaccharide derivative was superior to DHA in the time to reach the goal and the arrival rate, and addition of the sialic acid-containing oligosaccharide derivative in the infancy was not effective in the brain. He was involved in development, and as a result, exerted an excellent effect on acquisition of learning ability.
【００１０】試験例２ 実施例１及び２で得られた本発明品である学習能力組成
物（シアル酸含有オリゴ糖誘導体）の学習能力向上効果
を調べるため乳児マウスを用いて実験を行った。条件付
け刺激後に与えられた電撃をレバー押しによって回避す
る非連続能動回避学習、いわゆるオペラント学習の成立
過程を確認し、その過程に対する本発明品である学習能
力組成物（シアル酸含有オリゴ糖誘導体）の効果を検討
した。まず、７日令のＳＤマウスにサンプルを飼料中に
規定量添加しておく。飼料には標準飼料にシアル酸含有
オリゴ糖誘導体（実施例品１及び２）で得られた本発明
品それぞれ、０．５％，ガングリオシド０．５％，生理
食塩水をそれぞれ添加し実験を行った。２１日令から１
週間毎日刺激回避のためにレバーを押した回数を測定
し、各群の平均回避率を計算した。結果を表１に示す。Test Example 2 Experiments were carried out using infant mice in order to examine the learning ability improving effect of the learning ability compositions (sialic acid-containing oligosaccharide derivatives) of the present invention obtained in Examples 1 and 2. The establishment process of discontinuous active avoidance learning that avoids electric shock given after conditioning stimulus by pushing the lever, so-called operant learning, was confirmed, and the learning ability composition (sialic acid-containing oligosaccharide derivative) of the present invention for the process was confirmed. The effect was examined. First, a predetermined amount of a sample is added to the diet of 7-day-old SD mice. Experiments were carried out by adding 0.5%, gangliosides 0.5%, and physiological saline to the standard feeds of the present invention products obtained from the sialic acid-containing oligosaccharide derivatives (Example products 1 and 2), respectively. It was 1 from 21 days of age
The number of times the lever was pushed for avoidance of stimuli was measured every day of the week, and the average avoidance rate of each group was calculated. The results are shown in Table 1.
【００１１】[0011]
【表１】 [Table 1]
【００１２】表１より明らかなように本発明品である学
習能力組成物（シアル酸含有オリゴ糖誘導体）は、ガン
グリオシド以上に刺激に対する平均回避率が高く、乳児
期でのシアル酸含有オリゴ糖誘導体の添加は脳の発達に
関与し、結果的に学習能力の獲得に優れた効果を発揮し
た。As is clear from Table 1, the learning ability composition (sialic acid-containing oligosaccharide derivative) of the present invention has a higher average avoidance rate against stimuli than ganglioside, and the sialic acid-containing oligosaccharide derivative in infancy. Was added to the brain development, and as a result, it exerted an excellent effect on the acquisition of learning ability.
【００１３】本発明の実施態様をあげれば以下の通りで
ある。 （１）シアル酸含有オリゴ糖誘導体を有効成分として含
有することを特徴とする学習能力向上組成物。 （２）シアル酸含有オリゴ糖誘導体を鶏卵由来であるこ
とを特徴とする前記（１）記載の学習能力向上組成物。 （３）シアル酸含有オリゴ糖誘導体を鶏卵卵黄由来であ
ることを特徴とする前記（１）記載の学習能力向上組成
物。 （４）シアル酸含有オリゴ糖誘導体が、鶏卵卵黄から水
または塩溶液抽出物由来であることを特徴とする前記
（１）記載の学習能力向上組成物。 （５）鶏卵卵黄または前記（４）記載の抽出液を酵素消
化して得たことを特徴とするシアル酸含有オリゴ糖誘導
体を有効成分とした学習能力向上組成物。 （６）シアル酸含有オリゴ糖誘導体が、シアル酸残基を
１残基以上含んだ化合物である前記（１）〜（５）記載
の学習能力向上組成物。The embodiments of the present invention are as follows. (1) A learning ability improving composition comprising an oligosaccharide derivative containing sialic acid as an active ingredient. (2) The learning ability improving composition according to (1) above, wherein the sialic acid-containing oligosaccharide derivative is derived from an egg. (3) The learning ability improving composition as described in (1) above, wherein the sialic acid-containing oligosaccharide derivative is derived from chicken egg yolk. (4) The learning ability improving composition according to (1) above, wherein the sialic acid-containing oligosaccharide derivative is derived from water or salt solution extract from chicken egg yolk. (5) A composition for improving learning ability comprising an sialic acid-containing oligosaccharide derivative as an active ingredient, which is obtained by enzymatically digesting hen egg yolk or the extract described in (4) above. (6) The learning ability improving composition according to the above (1) to (5), wherein the sialic acid-containing oligosaccharide derivative is a compound containing one or more sialic acid residues.
【００１４】（７）シアル酸含有オリゴ糖誘導体が、鶏
卵を起源とした材料から調製されたことを特徴とする化
合物である前記（１）〜（６）記載の学習能力向上組成
物。 （８）シアル酸含有オリゴ糖誘導体が、アミノ酸残基を
１残基以上含んだ化合物である前記（１）〜（７）記載
の学習能力向上組成物。 （９）シアル酸含有オリゴ糖誘導体が、アミノ酸残基を
含まない遊離オリゴ糖である前記（１）〜（８）記載の
学習能力向上組成物。 （１０）塩溶液の塩濃度が、０−０．５Ｍの濃度である
前記（４）〜（５）記載の学習能力向上組成物。 （１１）酵素が、糖分解酵素である前記（５）記載の学
習能力向上組成物。 （１２）酵素が、蛋白分解酵素である前記（５）記載の
学習能力向上組成物。 （１３）酵素が、脂質分解酵素である前記（５）記載の
学習能力向上組成物。 （１４）蛋白分解酵素としては、Aspergillus 属、 Bac
illus 属などの産生するプロテアーゼである前記（５）
記載の学習能力向上組成物。(7) The learning ability improving composition according to the above (1) to (6), which is a compound characterized in that the sialic acid-containing oligosaccharide derivative is prepared from a material derived from chicken eggs. (8) The learning ability improving composition according to the above (1) to (7), wherein the sialic acid-containing oligosaccharide derivative is a compound containing one or more amino acid residues. (9) The learning ability improving composition according to the above (1) to (8), wherein the sialic acid-containing oligosaccharide derivative is a free oligosaccharide containing no amino acid residue. (10) The learning ability improving composition according to the above (4) to (5), wherein the salt solution has a salt concentration of 0 to 0.5M. (11) The learning ability improving composition according to the above (5), wherein the enzyme is a glycolytic enzyme. (12) The learning ability improving composition according to the above (5), wherein the enzyme is a protease. (13) The learning ability improving composition according to the above (5), wherein the enzyme is a lipolytic enzyme. (14) As a protease, Aspergillus genus, Bac
The above (5), which is a protease produced by the genus illus
The composition for improving learning ability described.
【００１５】（１５）鶏卵卵黄から有効成分であるシア
ル酸含有オリゴ糖誘導体を抽出し、学習能力向上作用を
有した組成物。 （１６）鶏卵卵黄から有効成分であるシアル酸含有オリ
ゴ糖誘導体を水で抽出し、学習能力向上作用を有した組
成物。 （１７）鶏卵卵黄から有効成分であるシアル酸含有オリ
ゴ糖誘導体を塩溶液で抽出し、学習能力向上作用を有し
た組成物。 （１８）鶏卵卵黄の水抽出液から有効成分であるシアル
酸含有オリゴ糖誘導体を酵素消化で調製した学習能力向
上作用を有した組成物。 （１９）鶏卵卵黄の塩溶液抽出液から有効成分であるシ
アル酸含有オリゴ糖誘導体を酵素消化で調製した学習能
力向上作用を有した組成物。 （２０）鶏卵卵黄の水抽出液から有効成分であるシアル
酸含有オリゴ糖誘導体を蛋白分解酵素消化で調製した学
習能力向上作用を有した組成物。 （２１）鶏卵卵黄の塩溶液抽出液から有効成分であるシ
アル酸含有オリゴ糖誘導体を蛋白分解酵素消化で調製し
た学習能力向上作用を有した組成物。(15) A composition having a learning ability improving action by extracting an sialic acid-containing oligosaccharide derivative as an active ingredient from chicken egg yolk. (16) A composition having an ability to improve learning ability by extracting an sialic acid-containing oligosaccharide derivative which is an active ingredient from chicken egg yolk with water. (17) A composition having an action of improving learning ability by extracting a sialic acid-containing oligosaccharide derivative which is an active ingredient from chicken egg yolk with a salt solution. (18) A composition having an ability to improve learning ability, which is prepared by enzymatically digesting a sialic acid-containing oligosaccharide derivative which is an active ingredient from a water extract of chicken egg yolk. (19) A composition having an ability to improve learning ability, which is prepared by enzymatically digesting a sialic acid-containing oligosaccharide derivative as an active ingredient from a salt solution extract of chicken egg yolk. (20) A composition having an ability to improve learning ability, which is prepared by proteolytically digesting an oligosaccharide derivative containing sialic acid, which is an active ingredient, from a water extract of chicken egg yolk. (21) A composition having an ability to improve learning ability, which is prepared by proteolytically digesting a sialic acid-containing oligosaccharide derivative which is an active ingredient from a salt solution extract of chicken egg yolk.
【００１６】[0016]
【発明の効果】以上のように本発明品である鶏卵卵黄か
ら調製したシアル酸含有オリゴ糖誘導体が学習能力を向
上することができる。さらに、本発明品は、医薬品素
材、機能性食品素材として使用される際、ドコサヘキサ
エン酸などと比較し、臭いや味に影響を与えることなく
その効果を強く発揮する化合物である。本発明品を用い
ることは効果、安全性の点をも考え併せて産業界にとっ
て極めて有益である。As described above, the sialic acid-containing oligosaccharide derivative prepared from the egg yolk of the present invention can improve the learning ability. Furthermore, the product of the present invention is a compound that exerts its effect strongly without affecting odor or taste when used as a drug material or a functional food material, as compared with docosahexaenoic acid. The use of the product of the present invention is extremely beneficial to the industrial world in consideration of effects and safety.
【００１７】[0017]
【図１】迷路の平面図である。FIG. 1 is a plan view of a maze.
【図２】ゴールまでの到達率（％）の図である。FIG. 2 is a diagram of a reaching rate (%) to a goal.
【図３】ゴールまでの到達時間の図である。FIG. 3 is a diagram of arrival time to a goal.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 金 武祚 三重県四日市市赤堀新町９番５号 太陽化 学株式会社内 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Kim Takehisa 9-5 Akahori Shinmachi, Yokkaichi-shi, Mie Taiyo Kagaku Co., Ltd.
Claims (3)
ことを特徴とする学習能力向上組成物。1. A composition for improving learning ability, which comprises a sialic acid-containing oligosaccharide derivative.
黄より得られたものであることを特徴とする請求項１記
載の学習能力向上組成物。2. The learning ability improving composition according to claim 1, wherein the sialic acid-containing oligosaccharide derivative is obtained from chicken egg yolk.
して得たことを特徴とする請求項１，２記載の学習能力
向上組成物。3. The learning ability improving composition according to claim 1, wherein the sialic acid-containing oligosaccharide derivative is obtained by enzymatic digestion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7099779A JPH08266255A (en) | 1995-03-30 | 1995-03-30 | Composition for improving learning ability |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7099779A JPH08266255A (en) | 1995-03-30 | 1995-03-30 | Composition for improving learning ability |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH08266255A true JPH08266255A (en) | 1996-10-15 |
Family
ID=14256440
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7099779A Pending JPH08266255A (en) | 1995-03-30 | 1995-03-30 | Composition for improving learning ability |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH08266255A (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003300891A (en) * | 2002-04-10 | 2003-10-21 | Taiyo Kagaku Co Ltd | Cerebral function ameliorating composition |
| EP2116139A1 (en) * | 2008-05-08 | 2009-11-11 | Nestec S.A. | Sialic acid to support brain health in the elderly |
| US8703737B2 (en) | 2010-12-31 | 2014-04-22 | Abbott Laboratories | Nutritional formulations including human milk oligosaccharides and antioxidants and uses thereof |
| US8802650B2 (en) | 2010-12-31 | 2014-08-12 | Abbott Laboratories | Methods of using human milk oligosaccharides for improving airway respiratory health |
| US9283240B2 (en) | 2010-12-31 | 2016-03-15 | Abbott Laboratories | Human milk oligosaccharides for modulating inflammation |
| EP1874336B1 (en) | 2005-04-29 | 2016-04-27 | MJN U.S. Holdings LLC | A method of improving learning and memory in an infant comprising administering casein glycomacropeptide |
| US9539269B2 (en) | 2010-12-31 | 2017-01-10 | Abbott Laboratories | Methods for decreasing the incidence of necrotizing enterocolitis in infants, toddlers, or children using human milk oligosaccharides |
| US9763970B2 (en) | 2010-12-31 | 2017-09-19 | Abbott Laboratories | Nutritional compositions comprising human milk oligosaccharides and nucleotides and uses thereof for treating and/or preventing enteric viral infection |
| US9795623B2 (en) | 2010-12-31 | 2017-10-24 | Abbott Laboratories | Methods for reducing the incidence of oxidative stress using human milk oligosaccharides, vitamin C and anti-inflammatory agents |
| KR20190019064A (en) * | 2016-06-14 | 2019-02-26 | 쏘나피 (쏘시에떼 빠르 악시옹 쌩쁠리피에) | Anhydrous food based on cocoa butter and dried plant material |
| US10639319B2 (en) | 2011-08-29 | 2020-05-05 | Abbott Laboratories | Human milk oligosaccharides for preventing injury and/or promoting healing of the gastrointestinal tract |
| US11337990B2 (en) | 2010-12-31 | 2022-05-24 | Abbott Laboratories | Human milk oligosaccharides to promote growth of beneficial bacteria |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4535662B2 (en) * | 2002-04-10 | 2010-09-01 | 太陽化学株式会社 | Brain function improving composition |
| JP2003300891A (en) * | 2002-04-10 | 2003-10-21 | Taiyo Kagaku Co Ltd | Cerebral function ameliorating composition |
| EP1874336B1 (en) | 2005-04-29 | 2016-04-27 | MJN U.S. Holdings LLC | A method of improving learning and memory in an infant comprising administering casein glycomacropeptide |
| EP2116139A1 (en) * | 2008-05-08 | 2009-11-11 | Nestec S.A. | Sialic acid to support brain health in the elderly |
| US10369164B2 (en) | 2010-12-31 | 2019-08-06 | Abbott Laboratories | Nutritional formulations including human milk oligosaccharides and antioxidants and uses thereof |
| US10471081B2 (en) | 2010-12-31 | 2019-11-12 | Abbott Laboratories | Methods for decreasing the incidence of necrotizing enterocolitis in infants, toddlers, or children using human milk oligosaccharides |
| US8802650B2 (en) | 2010-12-31 | 2014-08-12 | Abbott Laboratories | Methods of using human milk oligosaccharides for improving airway respiratory health |
| US9539269B2 (en) | 2010-12-31 | 2017-01-10 | Abbott Laboratories | Methods for decreasing the incidence of necrotizing enterocolitis in infants, toddlers, or children using human milk oligosaccharides |
| US9763970B2 (en) | 2010-12-31 | 2017-09-19 | Abbott Laboratories | Nutritional compositions comprising human milk oligosaccharides and nucleotides and uses thereof for treating and/or preventing enteric viral infection |
| US9795623B2 (en) | 2010-12-31 | 2017-10-24 | Abbott Laboratories | Methods for reducing the incidence of oxidative stress using human milk oligosaccharides, vitamin C and anti-inflammatory agents |
| US9808474B2 (en) | 2010-12-31 | 2017-11-07 | Abbott Laboratories | Nutritional formulations including human milk oligosaccharides and antioxidants and uses thereof |
| US11633412B2 (en) | 2010-12-31 | 2023-04-25 | Abbott Laboratories | Nutritional formulations including human milk oligosaccharides and antioxidants and uses thereof |
| US8703737B2 (en) | 2010-12-31 | 2014-04-22 | Abbott Laboratories | Nutritional formulations including human milk oligosaccharides and antioxidants and uses thereof |
| US9283240B2 (en) | 2010-12-31 | 2016-03-15 | Abbott Laboratories | Human milk oligosaccharides for modulating inflammation |
| US11524018B2 (en) | 2010-12-31 | 2022-12-13 | Abbott Laboratories | Nutritional formulations including human milk oligosaccharides and antioxidants and uses thereof |
| US10709720B2 (en) | 2010-12-31 | 2020-07-14 | Abbott Laboratories | Human milk oligosaccharides for modulating inflammation |
| US10813940B2 (en) | 2010-12-31 | 2020-10-27 | Abbott Laboratories | Nutritional compositions comprising human milk oligosaccharides and nucleotides and uses thereof for treating and/or preventing enteric viral infection |
| US10973837B2 (en) | 2010-12-31 | 2021-04-13 | Abbott Laboratories | Nutritional formulations including human milk oligosaccharides and antioxidants and uses thereof |
| US11197875B2 (en) | 2010-12-31 | 2021-12-14 | Abbott Laboratories | Nutritional formulations including human milk oligosaccharides and antioxidants and uses thereof |
| US11207335B2 (en) | 2010-12-31 | 2021-12-28 | Abbott Laboratories | Methods of using human milk oligosaccharides for improving airway respiratory health |
| US11311562B2 (en) | 2010-12-31 | 2022-04-26 | Abbott Laboratories | Methods for reducing the incidence of oxidative stress using human milk oligosaccharides, vitamin c and anti-inflammatory agents |
| US11337990B2 (en) | 2010-12-31 | 2022-05-24 | Abbott Laboratories | Human milk oligosaccharides to promote growth of beneficial bacteria |
| US10639319B2 (en) | 2011-08-29 | 2020-05-05 | Abbott Laboratories | Human milk oligosaccharides for preventing injury and/or promoting healing of the gastrointestinal tract |
| KR20190019064A (en) * | 2016-06-14 | 2019-02-26 | 쏘나피 (쏘시에떼 빠르 악시옹 쌩쁠리피에) | Anhydrous food based on cocoa butter and dried plant material |
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