JP3160862B2 - Bone-fortified foods, feeds and pharmaceuticals - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD OF THE INVENTION

The present invention relates to a bone-enhancing food, a bone-enhancing feed or a bone-enhancing agent containing a water-soluble fraction of whey protein or a water-soluble fraction of a proteolytic enzyme degradation product thereof. Furthermore, the present invention relates to a bone-enhancing food, a bone-enhancing feed or a bone-enhancing agent obtained by using an appropriate calcium salt in combination with these water-soluble fractions.

[0002]

[Prior art]

In recent years, as the population ages, the number of patients suffering from various bone diseases such as osteoporosis, fractures and back pain is increasing. It is said insufficient intake of calcium, lowering of calcium absorbing ability, and the like imbalance deficiency and hormone-activated vitamin D ₃ secretion is the cause. Currently calcium carbonate for the purpose of supplementing calcium,
Calcium salts such as calcium lactate and calcium phosphate and natural calcium agents such as bovine bone meal, eggshell and fish bone powder are used. Pharmaceuticals for osteoporosis and bone strengthening include vitamin 1α (OH) D ₃ and calcitonin preparations. However, these are medicines themselves, safe and long-term ingestion under mild conditions used for food materials,
No known bone strengthening substances are known.

[0003]

[Problems to be solved by the invention]

Milk is excellent as a natural material, but even if milk is taken as food or medicine as it is, a large amount of whey proteins and peptides cannot be consumed. The present invention has been made for the purpose of utilizing such conventional milk-derived whey proteins and peptides and for strengthening bones. That is, an object of the present invention is to provide a bone-enhancing food, a bone-enhancing feed, and a bone-enhancing agent that can absorb a large amount of whey proteins and peptides and can strengthen bone.

[0004]

[Means for Solving the Problems]

The present inventors have studied the production of foods, feeds, and medicines that can absorb whey proteins and peptides and can also promote calcium absorption from whey. As a result, the water-soluble fraction of whey or
A water-soluble fraction obtained by hydrolysis with a protease, or a protein mixture obtained by removing whey-derived salts from these fractions by a reverse osmosis (RO) membrane or electrodialysis (ED), etc.
They have the effect of strengthening bones, and have found that calcium absorption can be further promoted by adding a calcium agent having good absorbability to these.

[0005] Based on these findings, a food, medicine or feed having an action of strengthening bones has been obtained. That is, the present invention relates to the following bone-enhancing food, bone-enhancing feed, and bone-enhancing agent. (1) A bone-enhancing food, a bone-enhancing feed or a bone-enhancing agent having a molecular weight of 45,000 to 10,000 and an isoelectric point of p13 to 8 and containing a water-soluble fraction of whey protein. (2) A bone-enhancing food, a bone-enhancing feed or a bone-enhancing agent comprising the water-soluble fraction of whey protein according to claim 1 and a calcium salt having good absorbability. (3) A bone-fortifying feed or a bone-strengthening agent obtained by enzymatically decomposing a water-soluble fraction of whey protein with a protease and containing the resulting water-soluble fraction of a protease having a molecular weight of 20,000 to 300. . (4) A bone-strengthening feed or a bone-strengthening agent comprising a water-soluble fraction comprising the proteolytic enzyme hydrolyzate according to claim 3 and a calcium salt having good absorbability.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION

The raw material milk in the present invention includes milk, human milk, goat milk, sheep milk and the like. Whey is a clear yellow-green liquid that is free of coagulum produced by adding acid or rennet to milk or skim milk, and is obtained as a by-product of cheese or casein production. The whey protein in the present invention is a water-soluble protein obtained by subjecting this whey to ultrafiltration, reverse osmosis, chromatography, dialysis and the like. In particular, a water-soluble fraction of whey protein having a molecular weight of 10,000 to 45,000 and an isoelectric point of pI 3 to 8 is desirable.

In the present invention, this fraction is used as it is, or after being desalted and concentrated or dried. In the case of performing enzymatic degradation using a proteolytic enzyme, a proteolytic enzyme is added to this solution or suspension to perform enzymatic degradation. Pepsin, trypsin, chymotrypsin and the like can be used as the protease. The enzyme reaction is carried out by preparing a solution or a suspension so as to have an optimum pH and an optimum temperature of the enzyme to be used, adding the enzyme, and allowing the mixture to stand or stir for several tens of minutes to several hours. In this way, the molecular weight is 20,000-300
A degree of proteolytic degradation can be obtained. This proteolytic enzyme hydrolyzate is composed of whey protein or peptide.

In the present invention, it is desirable to add a calcium salt having good absorbability to the water-soluble fraction. At this time, it is preferable to add a calcium salt having good absorbability such as calcium chloride, calcium carbonate, calcium lactate, calcium derived from eggshell or milk, or a content thereof. In the present invention, such a calcium salt-containing substance having good absorbability is referred to as a calcium salt having good absorbability.

The bone-enriched food, bone-enriched feed or bone-enhancing agent of the present invention comprises a water-soluble fraction of these, a water-soluble fraction comprising an enzymatically degraded product of a water-soluble fraction by a protease, or a water-soluble fraction thereof. It contains a product obtained by adding a calcium salt having good absorbability to the fraction. Food examples include beverages, jellies, tablets, breads,
There are noodles, soups, sausages and the like, and the feed includes tablets, granules, liquids and the like which can be orally administered with feed additives and other feeds as a bone strengthening agent. These meanings are orally administered and used for prevention or treatment of osteoporosis (osteoporosis). The dosage is for adults,
It is desirable to administer about 4000 to 8000 mg / day in several divided doses. The fraction was originally a component of milk, and no acute toxicity was observed in animal tests using rats.

Next, a method for producing the whey peptide and the protein of the present invention will be described with reference to examples.

Example 1 Preparation method of whey protein (WP-N) 6N hydrochloric acid was dropped into skim milk 100 to pH 4.7. After standing for 1 hour, the precipitated casein was removed by centrifugation to obtain whey. This whey is coated with DOW UF membrane GR61PP with a membrane area of 0.72 m ²
(Fraction molecular weight: 20,000 daltons). The whey protein was further purified by diafiltration. The purified liquid of 4.31 was sufficiently dialyzed against distilled water.
After dialysis, freeze-dried and powder containing whey protein fraction (WP) 46
8 g were obtained.

The WP is applied to a G-100 column of 90 mm × 500 mm and fractionated, and the fraction is fractionated. Whey protein (WP-N) 18 having a molecular weight cutoff of 10,000 to 45,000 is used.
0 g was obtained.

Example 2 Preparation of Whey Protein Tryptic Hydrolyzate (WP-T) Whey protein (WP-T) obtained by the method described in Example 1
N) 200 g was dissolved in 4.5 distilled water.

[0012] 4 g of trypsin was added thereto and reacted at 37 ° C. for 5 hours to produce a whey protein trypsin hydrolyzate. Thereafter, the enzyme was inactivated by holding at 84 ° C. for 5 minutes, freeze-dried, and powdered with whey protein trypsin hydrolyzate (WP-T) 19
2 g were obtained. Its molecular weight was between 20,000 and 300.

[0013]

Example 3 Bone-Strengthening Effect of Whey Protein (WP-N) and Whey Protein Trypsin Degradate (WP-T) (1) Composition of Rat Feed Added with Test Sample The results are shown in Table 2 and Table 2. Feed basic ingredients (g / 100g) Sucrose 49 Corn starch 15 Corn oil 5 DL methionine 0.3 Cellulose 5 Vitamin 1.2 (including choline)

[0014]

As shown in Table 2, WP, WP-N and WP-T obtained in Examples 1 and 2 were separately added to feed at 1.09%, 1%,
1% was added, and the amount of protein in the feed was adjusted to 20% with casein. Dietary calcium level was g
It was adjusted with calcium carbonate so that the amount of calcium per was 300 mg. Adjustments were made so that phosphorus was 400 mg, potassium was 350 mg, magnesium was 80 mg, and sodium was 100 mg. (2) Preparation of used animals and osteoporosis model animals As the animals, 5-week-old SD female rats were used. Osteoporosis model rats were prepared by preliminarily breeding for one week, performing oophorectomy, and breeding on a low calcium diet for one month. The test was performed with 7 animals in each test group. (3) Bone strengthening test After osteoporosis model rats were bred for one month with the above-mentioned test feed, the femur was excised and the bone strength was measured with a fracture characteristics measuring device. (4) Test results

The test results are shown in FIG. 1 and FIG. As shown in FIG. 1, the bone breaking force was significantly higher in the group to which whey protein (WP-N) was added and the group to which whey protein trypsin hydrolyzate (WP-T) was added, as compared with the control group. showed that. The bone breaking energy shown in FIG. 2 was also higher than the control group and the low calcium group in WP-N
And the group to which WP-T was added showed remarkably high values. Moreover, the value was higher than that of the sham CaCO ₃ added group. From this, it was found that whey protein (WP-N) and whey protein trypsin hydrolyzate (WP-T) have a bone strengthening effect.

[0017] The food, feed or medicine of the present invention will be specifically described with increasing working age.

Example 4 (Beverage containing WP-N or WP-T)% by weight Mixed isomerized sugar 15.0 Fruit juice 10.0 Citric acid 0.5 WP-N or WP-T 0.5 Flavor 0.1 Calcium (CaCl ₂ ) 0.1 Water 73.8 Fruit juice beverages were produced according to the ratio by a usual production method.

[0018]

[Example 5] (Jelly containing WP-N or WP-T) wt% fruit juice 20.0 gnu sugar 15.0 water candy 5.0 agar 1.0 agar 1.0 WP-N or WP-T 0.5 flavor 0.1 calcium (CaCO ₃ ) 0.1 water 58.3 Jelly was produced according to the usual production method according to the compounding ratio.

[0019]

[Example 6] (Tablet containing WP-N or WP-T)% by weight Hydrous crystalline glucose 73.5 WP-N or WP-T 20.0 Calcium (CaCl ₂ ) 5.0 Sugar ester 1.0 Fragrance 0.5 Normal production method based on the above mixing ratio To produce tablets. The resulting tablets can be administered 5-6 tablets per day as a nutritional supplement for calcium and protein or 6-10 tablets per day for the prevention or treatment of osteoporosis.

[0020]

[Example 7] [Dog breeding feed (dog food)] Dog breeding basic feed weight% soybean meal 11 skim milk powder 14 soybean oil 4 corn oil 2 palm oil 2 corn starch 28 wheat flour 15 bran 8 vitamin mixture ¹⁾ 2 mineral mixture ²⁾ 9 Cellulose 2 WP-N or WP-T 3 100 ¹⁾ Vitamin mixture Vitamin A 1500 IU Vitamin D ₃ 300 IU Vitamin E 6.8 mg Vitamin B ₁ 0.9 mg Vitamin B ₂ 0.4 mg Vitamin B ₆ 0.5 mg Vitamin B ₁₂ 3.4 mg Vitamin C 50.0 mg Pantothenic acid 4.0 mg Folic acid 0.2 mg Choline 200.0 mg Biotin 24.4 μg Inositol 50.0 mg Niacin 10.5 mg Sucrose was added to 2 g. ²⁾ mineral mixture _{CaCO 3 3.0g KH 2 PO 4 2.0g} NaH 2 PO 4 1.5g MgO 0.5g MnCO 3 40.0mg FeC 6 H 5 O 7 30.0mg 70% ZnO 10.0mg 55% CaCO 3 4.5mg KIO 3 0.65mg was 9g with _{Na 2 SeO 3 · 5H 2 O} 0.05mg CrK (SO 4) · 12H 2 O 5.0mg sucrose. The vitamin mixture and the mineral mixture were added to the above-mentioned basic feed for dog breeding in an amount of 100 g in the above amount, and a feed for breeding dogs was produced by a usual production method.

[0021]

【The invention's effect】

The bone-fortified food, bone-strength feed, and bone-strengthening agent of the present invention are useful for preventing or treating health foods or various osteoarticular diseases, particularly osteoporosis, because they have the action of strengthening bone. It is also useful as feed.

[Brief description of the drawings]

1 and 2 show the results of the bone strengthening test of Example 3, respectively.

────────────────────────────────────────────────── ─── Continued on the front page (51) Int.Cl. ⁷ Identification code FI A23L 1/304 A23L 1/304 A61K 33/06 A61K 33/06 38/17 A61P 19/10 A61P 19/10 A23C 20/00 / A23C 20/00 C12P 21/06 C12P 21/06 A61K 37/16 (56) References JP-A-61-289846 (JP, A) JP-B-51-20578 (JP, B1) Monthly Food Chemical, (1987) ) Vol. 3, No. 6, p. 25-31 (58) Field surveyed (Int.Cl. ⁷ , DB name) A23L 1/305 A23J 3/08 A23J 3/34 A23L 1/304 A61K 38/17 JICST file (JOIS) JAFIC file (JOIS)

Claims (4)

(57) [Claims] (1) a molecular weight of 45,000 to 10,000 and an isoelectric point of p13 to
8. A bone-fortified food, a bone-strength feed or a bone-strengthening agent, which comprises a water-soluble fraction of whey protein. 2. A bone-enhancing food, bone-enhancing feed or bone-enhancing agent comprising the water-soluble fraction of whey protein according to claim 1 and a calcium salt having good absorbability. 3. A fortified feed or bone fortification comprising enzymatically decomposing a water-soluble fraction of whey protein with a protease and containing the resulting water-soluble fraction comprising a protease digest having a molecular weight of 20,000 to 300. Agent. 4. A bone-strengthening feed or a bone-strengthening agent comprising a water-soluble fraction comprising the proteolytic enzyme-decomposed product according to claim 3 and a calcium salt having good absorbability.