JPH08220050A - Electrolyte analyzer - Google Patents

Abstract

PURPOSE: To use one kind of sample as a high- and lowconcentration solutions for plotting calibration curve and an internal standard solution so as to easily change the concentration ranges of the solutions for plotting calibration curve and the internal standard solution by changing the rate of dilution of the internal standard solution. CONSTITUTION: An internal standard solution used as a reagent is used as a low-concentration solution for plotting calibration curve and a solution obtained by diluting the internal standard solution at a rate of dilution of 1.5-3.0 is used as a low-concentration curve for plotting calibration curve. Therefore, only three kinds of solutions of the internal standard solution, diluted internal standard solution, and comparison electrode solution are required. Namely, a calibration curve is plotted by automatically setting the concentration of the low- concentration solution by inputting the rate of dilution of the internal standard solution on the screen of a CRT. Then, by alternately measuring the diluted sample of a specimen and internal standard solution, the concentration of the specimen is calculated from the slope sensitivity and the potential difference between the internal standard solution and specimen. Therefore, no special reagent is required for plotting calibration curve and automatic calibration can be performed. In addition, the concentration range of calibration curves and the concentration of the internal standard solution can be easily changed.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an electrolyte analyzer by the ion electrode method.

[0002]

2. Description of the Related Art A flow chart of a typical conventional measurement procedure is shown in FIG. In the prior art, a calibration curve is created by measuring two kinds of reagents, a low-concentration solution and a high-concentration solution for creating a calibration curve. In this method, four kinds of reagents, a high-concentration solution for preparing a calibration curve, a low-concentration solution for preparing a calibration curve, a diluting solution, an internal standard solution, and a reference electrode solution, are required at least for calculating the concentration of a sample.

[0003]

In the prior art, in order to change the measurement conditions, it took a great deal of time for the above-mentioned various kinds of reagents and complicated manual operation.

An object of the present invention is to simplify the change of the calibration curve concentration range / internal standard solution concentration, the reagent management, and the routine cost reduction.

[0005]

To achieve the above object, the present invention changes the dilution ratio of the reagent of the internal standard solution for correcting potential drift.

[0006]

According to the present invention, by changing the dilution ratio of the internal standard solution for correcting potential drift, one kind of reagent serves as a high-concentration solution for preparing a calibration curve, a low-concentration solution for preparing a calibration curve, and an internal standard solution. It is possible to easily change the linear concentration range and the concentration of the internal standard solution.

[0007]

EXAMPLE A first example of the present invention is a clinical electrolyte analyzer. The flowchart of the measurement procedure is shown in FIG. 2, and the system diagram is shown in FIG. Sample dispensing nozzle 1, dilution tank 2, sodium / potassium / chlorine ion three-item integrated electrode 3, reference electrode 4, sipper syringe 5, diluent syringe 7, two-way solenoid valve for integrated electrode 8, two for reference electrode liquid One-way solenoid valve 9, two-way solenoid valve 10 for sipper syringe suction, two-way solenoid valve 11 for sipper syringe waste liquid, two-way solenoid valve 14 for diluting liquid discharge,
Two-way solenoid valve for diluting liquid suction, reference electrode liquid bottle 16,
Diluent bottle 18, two-way solenoid valve for discharging internal standard solution 19,
It is composed of an internal standard solution syringe 20, an internal standard solution suction two-way solenoid valve 21, and an internal standard solution bottle 22. The electromotive force of the ion-selective electrode is represented by Equation 1.

[0008]

[Equation 1] E = E ₀ + SL·logC (Equation 1) E: Measurement potential E ₀ : Reference potential SL: Slope sensitivity C: Concentration In the case of blood electrolyte measurement, from the clinician side, the measurement accuracy is 1 % Is required, which is
For example, in the case of measuring sodium ion in serum,
Since the normal range (reference range) is around 135 to 146 mmol / l, it is within ± 1.4 mmol / l. Therefore, the measurement error due to the device is required to be at least ± 0.5 mmol / l or less. The error of ± 0.5 mmol / l is within ± 0.1 mV from Eq. 2 when converted to electromotive force.

[0009]

[Equation 2]

From equation 3, slope sensitivity is 60 mV / decade
When the internal standard solution for electric potential correction was used as the high-concentration range sample, the difference in electromotive force between the calibration curve low-concentration solution (120 mMol / l) and the high-concentration solution (140 mmol / l) was 4.02 mV.
Is.

[0011]

(Equation 3)

The ratio of the low concentration liquid to the high concentration liquid in the calibration curve at this time is 1.31 from the equation (4).

[0013]

[Equation 4]

Similarly, the required concentration ratios described above for potassium ions and chlor ions can be calculated. Measurement error due to each device is ± 0.05mmol / l, ± 0.5mmol
Assuming 1 / l, the calibration curves for potassium ion and chlorion ion are respectively 1.26 and 1.3 for the high concentration liquid and the low concentration liquid.
It becomes 9. From this fact, when simultaneously measuring sodium, potassium and chlorion, the ratio of the dilution ratio of the calibration curve low concentration liquid and the high concentration liquid must be 1.39 or more. The ratio was set to 1.5.
Since the dilution ratio of the high concentration liquid is 1, the minimum effective dilution ratio is 1.5. In addition, the lower limit of the calibration curve low concentration is 30 mMol / l, 1 mMol / l, 30 mMol for sodium ion, potassium ion, and chlorion ion, respectively, from the results of studying the concentration that can obtain a stable linear range.
/ L, and the sodium, potassium, and chlorion concentrations of the internal standard solution are 140.0, 5.0, 100.
Since it is mmol / l, the lower limit of chlor was considered to be the limit, and the upper limit of the dilution ratio was set to 3 times. In the reagent used in the present invention, the internal standard solution also serves as the high-concentration-calibration-concentration solution, and the diluted internal standard solution is used as the low-calibration-curve solution. That is,
The required reagents are the internal standard solution, diluent, and reference electrode solution.
It is a kind. By inputting an arbitrary dilution ratio of the internal standard solution on the screen of the CRT, the concentration of the low-concentration liquid for preparing the calibration curve is automatically set and the calibration curve is prepared. The diluted sample of the sample and the internal standard solution are measured alternately. Dispense amount of sample is 20μ
1, the diluent was 580 μl, and the dilution ratio was fixed at 30 times. The sample concentration is calculated from the slope sensitivity and the potential difference between the internal standard solution and the sample. The composition of the reagents used is shown in Table 1, and the dilution factor setting conditions for serum are shown in Table 2.

[0015]

[Table 1]

[0016]

[Table 2]

The second embodiment of the present invention is a clinical electrolyte analyzer capable of arbitrarily setting the concentration of the calibration curve producing reagent by changing the dilution ratio of the internal concentrated liquid. The flow chart of the measurement procedure is shown in FIG. 4, and the flow path diagram is shown in FIG.
It was shown to. The flow path is a sample dispensing nozzle 1, a diluting tank 2, sodium / potassium / chlorine ion three-item integrated electrode 3, a reference electrode 4, a sipper syringe 5, an internal concentrate syringe 6,
Diluent syringe 7, two-way solenoid valve for integrated electrode 8, two-way solenoid valve for reference electrode solution 9, two-way solenoid valve for suction of sipper syringe 10, two-way solenoid valve for waste of sipper syringe 11, discharge of internal concentrated solution One-way solenoid valve 12, two-way solenoid valve for sucking internal concentrated liquid 13, two-way solenoid valve for discharging diluting liquid 14, two-way solenoid valve for sucking diluted liquid 15, reference electrode liquid bottle 16, internal concentrated liquid bottle 17, diluting liquid A bottle 18, a two-way solenoid valve 19 for discharging an internal standard solution, an internal standard solution syringe 20, a two-way solenoid valve 21 for sucking an internal standard solution, and an internal standard solution bottle 22.

By inputting an arbitrary dilution ratio of the internal concentrate on the CRT screen, the concentrations of the low-concentration liquid for preparing the calibration curve and the high-concentration liquid for preparing the calibration curve are automatically set to prepare the calibration curve. The diluted sample of the sample and the internal standard solution are measured alternately.
The amount of the sample dispensed is 20 μl, the diluent is 580 μl, and the dilution ratio is fixed at 30 times. The sample concentration is calculated from the slope sensitivity and the potential difference between the internal standard solution and the sample. Table 3 shows the composition of the reagents used.

[0019]

[Table 3]

Table 4 shows the conditions for setting the dilution ratio for serum.

[0021]

[Table 4]

For example, for sodium ion items, 112
Create a calibration curve in the concentration range of ~ 168 mmol / l, and
The concentration of the sample is calculated from the potential difference from the internal standard solution of 0 mmol / l. Similarly, Table 5 shows the conditions for setting the dilution ratio for urine.

[0023]

[Table 5]

Compared with the calibration curve for serum, the concentration range of sodium ion items was expanded to 84 to 280 mmol / l.

The third embodiment of the present invention is a clinical electrolyte analyzer in which the calibration concentration range can be fixed or arbitrary. The flow chart is shown in FIG. 6 and the flow chart of the measurement procedure is shown in FIG. . The flow path is a sample dispensing nozzle 1, a diluting tank 2, a sodium / potassium / chlorine ion three-item integrated electrode 3, a reference electrode 4, a sipper syringe 5, an internal concentrated liquid syringe 6, a diluted liquid syringe 7, and a two-way integrated electrode. Solenoid valve 8,
Two-way solenoid valve for reference electrode liquid 9, two-way solenoid valve for sipper syringe suction, two-way solenoid valve for sipper syringe waste liquid 1
1, two-way solenoid valve for discharging internal concentrated liquid 12, two-way solenoid valve for sucking internal concentrated liquid 13, two-way electromagnetic valve for diluting liquid discharging 14, two-way solenoid valve for sucking diluting liquid 15, comparison electrode liquid bottle 16, It is composed of an internal concentrate bottle 17 and a diluent bottle 18.
By inputting an arbitrary dilution ratio of the internal concentrate on the CRT screen, the concentrations of the low-concentration solution for creating the calibration curve, the high-concentration solution for creating the calibration curve, and the internal standard solution are automatically set to create the calibration curve. The diluted sample of the sample and the internal standard solution are measured alternately.
The amount of the sample dispensed is 20 μl, the diluent is 580 μl, and the dilution ratio is fixed at 30 times. The sample concentration is calculated from the slope sensitivity and the potential difference between the internal standard solution and the sample. The composition of the reagents used is shown in Table 6.

[0026]

[Table 6]

First, the type of sample is selected from among three modes, serum, urine, and arbitrary. For serum and urine, Table 7,
The high-concentration solution, low-concentration solution, and internal standard solution for the calibration curve are automatically adjusted according to the dilution ratio in Table 8 to measure the sample.

[0028]

[Table 7]

[0029]

[Table 8]

When any is selected, 70 to 420 mmol / l for sodium ion, 2.5 to 15 mmol / l for potassium ion, and 50 to 3 for chlor ion.
If the concentration of any one of the high-concentration solution and the low-concentration solution for the calibration curve is arbitrarily set within the range of 00 mmol / l, the internal standard solution concentration is automatically set at the center of the calibration curve concentration range.
The measurement of the sample is performed.

[0031]

EFFECTS OF THE INVENTION A reagent for preparing a calibration curve is unnecessary, and automatic calibration is possible. Further, it is possible to easily change the concentration range of the calibration curve and the concentration of the internal standard solution. Facilitates reagent management and reduced routine costs.

[Brief description of drawings]

FIG. 1 is a flowchart of a conventional measurement procedure.

FIG. 2 is a flowchart of the first embodiment of the present invention.

FIG. 3 is a systematic diagram of an apparatus according to the first embodiment of the present invention.

FIG. 4 is a flowchart of the measurement procedure of the second embodiment of the present invention.

FIG. 5 is a system diagram of an apparatus according to a second embodiment of the present invention.

FIG. 6 is a system diagram of an apparatus according to a third embodiment of the present invention.

FIG. 7 is a flowchart of the measurement procedure of the third embodiment of the present invention.

[Explanation of symbols]

1 ... Nozzle for sample dispensing, 2 ... Diluting tank, 3 ... Sodium
Potassium / chlorine ion three-item integrated electrode, 4 ... comparison electrode,
5 ... Shipper syringe, 6 ... Internal concentrate syringe, 7 ...
Diluent syringe, 8 ... Two-way solenoid valve for integrated electrode, 9 ... Two-way solenoid valve for reference electrode solution, 10 ... Two-way solenoid valve for suction of sipper syringe, 11 ... Two-way solenoid valve for waste of sipper syringe,
12 ... Two-way solenoid valve for discharging internal concentrated solution, 13 ... Two-way solenoid valve for sucking internal concentrated solution, 14 ... Two-way solenoid valve for discharging diluted solution, 1
5 ... Two-way solenoid valve for sucking diluent, 16 ... Comparative electrode solution bottle, 17 ... Internal concentrate bottle, 18 ... Diluent bottle, 1
9 ... Two-way solenoid valve for discharging internal standard solution, 20 ... Internal standard solution syringe, 21 ... Two-way solenoid valve for sucking internal standard solution, 22 ... Internal standard solution bottle.

Claims (3)

[Claims] 1. An electrolyte analyzer according to the dilution potential difference method, characterized in that a high-concentration solution, a low-concentration solution and an internal standard solution for preparing a calibration curve are prepared by an internal standard solution for potential drift correction. 2. The concentration of the internal standard solution can be arbitrarily set within the concentration range of the high-concentration liquid and the low-concentration liquid for preparing the calibration curve by changing the dilution ratio from 1.5 to 3.0. Possible electrolyte analyzer. 3. The electrolyte analyzer according to claim 1, which has a function of selecting either fixed or arbitrary setting of the calibration concentration range.