JPH08219957A - Cell for crushing frozen sample - Google Patents

Abstract

PURPOSE: To recover crushed sample while containing in a container by constituting a cell of a base body having inner circumferential face corresponding, in profile, to the outer circumferential face of a tubular bottomed container body and a cover body having outer circumferential face corresponding to the inner circumferential face of the container body. CONSTITUTION: A base body 4 is combined with a container body 1 and a cover body 8 and cooled sufficiently down to -190 deg.C in a Dewar vessel filled with nitrogen before being taken out on a cork board 14 having low thermal conductivity. The cover body 8 is removed and a frozen biological sample is placed in the container body 1 and then the cover body 8 is applied again. Subsequently, the cover body 8 is subjected to oscillation by means of an oscillatory press 9 thus crushing the frozen sample. When the crushed sample is recovered, the cover body 8 is removed and then the container body 1 is removed from the base body 4 and inclined. When a tapered groove is made in one side of the container body 1, the crushed sample can be recovered without scattering.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention relates to crushing a frozen tissue sample when extracting or analyzing a specific component from a sample of muscle tissue, hard tissue such as collagen or fibrous tissue,
The present invention also relates to a frozen sample crushing cell that can be collected in a frozen state.

[0002]

2. Description of the Related Art Crushing various substances and extracting or analyzing a certain component from the crushed material are performed by many researchers or production sites. Among them, the analysis of a biological sample has been performed using a cell as shown in FIG. 7, for example, from the viewpoint that it is preferable to crush the biological sample in a frozen state.

That is, as shown in FIG. 1, a container / stand b in which a conical recess a is formed in a central portion is formed from a lid main body d in which a conical projection c having a shape corresponding to the concavity a is partially formed. Was configured. And in this thing, the said lid main body d
After the frozen sample was put into the concave portion a, the cone body projection c of the lid main body d was engaged with the concave portion a, and the lid main body d was vibrated by a vibration press to be crushed.

[0004]

However, when recovering the crushed sample from the container / stand b as described above, the sample is returned to room temperature after being put in the container / stand b, and is then placed in another container. The method of washing it off was adopted. This was to use the container / stand b for an imminent next application, but it is necessary to collect such a sample in a crushed state in a pure state without adding anything in order to prevent alteration. However, in order to do so, a large number of containers / stands b must be prepared. However, in the above-mentioned conventional example, the container / stands b and the lid body d are combined. Therefore, the above ideal could not be realized.

[0005]

Therefore, in order to solve the above-mentioned problems, the frozen sample crushing cell according to the present invention has an inner circumference substantially corresponding to the outer circumference of a container body having a cylindrical bottom. The base body has a surface in at least a part thereof, and the lid body has an outer peripheral surface having a shape substantially corresponding to the inner peripheral surface of the container body in at least a part.

[0006]

In other words, the container body, the base body, and the lid body are combined, and after cooling to about -190 degrees, the lid body is removed, the frozen sample is put in the container body, and the lid body is covered again. The sample is crushed from the lid body by a vibration press. Then, the crushed sample is collected in the state of being put in the container body.

[0007]

EXAMPLE An example of a frozen sample crushing cell according to the present invention will now be described with reference to the drawings, in which 1 is a cylindrical container body having a bottom, and the bottom 2 of the container body 1 is In order to enhance the crushing efficiency by concentrating the sample in the center of the bottom portion 2 by the vibration when crushing the sample to be described later, it is formed in a conical concave shape.

Reference numeral 3 denotes a tapered groove portion formed from the inner surface of the container body 1 to the upper edge thereof, and this groove portion 3 functions as a guide groove for collecting the crushed sample from the container body 1 as described above. .

Reference numeral 4 denotes a base body having an inner peripheral surface having a shape substantially corresponding to the outer peripheral surface of the container main body 1 in at least a part thereof. As shown in FIG. 2, the container main body 1 is formed in a cylindrical shape and has a lower surface. Is flat, the recess 5 of the base body 4 has a shape corresponding to that shape, and as shown in FIG. 3, when the container body 1 is cylindrical and the lower surface is pyramidal, The concave portion of the table body 4 is also formed in a shape corresponding to that shape. In addition, when the lower surface of the container body 1 is formed flat as shown in FIG. 2, vibration is applied from above as described later. As a result, the corners of the lower surface of the container body 1 are not Can be deformed outward in a
As a result, there is a possibility that the container body 1 may be attached to and detached from the base body 4 tightly. Therefore, in order to prevent this, it is preferable to form the lower surface of the container body 1 into a conical shape as shown in FIG. However, even when formed in this way, the lower surface of the container body 1 may be deformed due to the vibration with respect to the container body 1, and the deformation is deformed toward the center of the tip 6 of the lower surface of the container body 1. Since it is expected that the tip portion 6 will extend downward, a small concave portion 7 that is one step lower is formed in the center of the concave portion 5 of the base body 4 in order to avoid the extended portion. .

Reference numeral 8 denotes a lid main body having an outer peripheral surface having a shape substantially corresponding to the inner peripheral surface of the container main body 1 in at least a part thereof, and the container main body 1 has a cylindrical shape with a bottom formed in a conical recess. In the case of a shape, the lower surface of the lid body 8 is formed into a columnar shape having a conical convex portion.

Further, a recess 10 into which the vibration press 9 can be inserted is formed in the upper portion of the lid main body 8. However, if the vibration press 9 can be properly abutted, the recess 10 shown in the drawing is formed. There is no particular need to form it.

Reference numeral 11 denotes a hanging metal fitting that is rotatably connected to the base body 4. The hanging metal fitting 11 is in a state in which the container body 1 and the lid body 8 are engaged with the base body 4. It is formed in a gate shape having a height (depth) at which it can rotate. Reference numeral 12 is a hook metal fitting having a hook portion 13 formed at the tip thereof.
It is used to transport the table body 4 with the container body 1 and the lid body 8 engaged with each other.

Then, the container main body 1 and the lid main body 8 are stacked on the base main body 4 so as to engage with each other in a male and female state, and they are stored in a Dewar bottle containing liquid nitrogen until the temperature becomes about -190.degree. After cooling, the product is taken out onto an object having a low thermal conductivity, for example, a cork plate 14. When taking out in this way, the hanging metal fittings 12 arranged on the base body 4 by the above-mentioned hanging metal fittings 12 are used.

Next, the lid main body 8 is removed, the cooled biological sample is put into the container main body 1, the lid main body 8 is engaged again, and the vibration press 9 is brought into contact with the lid main body 8 to attach it to the lid main body 8. By vibrating, the frozen sample will be crushed.

When recovering the crushed sample, the lid body 8 may be removed, the container body 1 may be removed from the base body 4, and the container body 1 may be tilted.
By forming the above-mentioned groove portion 3 in the above, it becomes possible to collect in a state where it is not scattered.

[0016]

As described above, according to the frozen sample crushing cell of the present invention, at least a part thereof has an inner peripheral surface having a shape substantially corresponding to the outer peripheral surface of a container body formed in a cylindrical bottomed shape. The crushed sample can be collected in the state of being housed in the container body because it is composed of the base body and the lid body having at least a part of the outer peripheral surface of a shape substantially corresponding to the inner peripheral surface of the container main body. Also, it is possible to prevent alteration of the sample, and it is possible to use the same base body and lid body if a plurality of container bodies are prepared.

[Brief description of drawings]

FIG. 1 is a perspective view showing a state in which a frozen sample crushing cell according to the present invention is separated.

FIG. 2 is a sectional view taken along the line AA of FIG.

FIG. 3 is a perspective view showing an example in which a recess is formed in the base body and the lower surface of the container body is formed in a pyramidal shape.

FIG. 4 is a perspective view showing another example of the container body.

FIG. 5 is a cross-sectional view showing a state where a hanging metal fitting is arranged on the base body.

FIG. 6 is a perspective view of a hook.

FIG. 7 is a sectional view showing a conventional example.

[Explanation of symbols]

 1 Container Main Body 2 Bottom Part 3 Groove Part 4 Unit Main Part 5 Recessed Part 6 Pointed Part 7 Small Recessed Part 8 Lid Main Body 9 Vibration Press 10 Recessed Part 11 Hanging Fitting 12 Hooking Fitting 13 Hook Part 14 Cork Board

Claims (1)

[Claims] 1. A base body having at least a portion of an inner peripheral surface having a shape substantially corresponding to the outer peripheral surface of a container body formed in a cylindrical bottom shape, and an outer periphery having a shape substantially corresponding to the inner peripheral surface of the container body. A frozen sample crushing cell comprising a lid main body having a surface in at least a part thereof.