JPH08206685A - Waste water treatment - Google Patents

Abstract

PURPOSE: To efficiently treat high concentration waste water and at the same time lessen the production quantity of excessive sludge by basically treating waste water by utilizing yeast and yeast-lysing bacterium. CONSTITUTION: Waste water is treated by utilizing yeast and yeast-lysing bacterium. That is, at first waste water purification is carried out by employing enzymes having high decomposing function against suspending substances in the waste water. Then, yeast propagated following the purification, is lysed by yeast-lysing bacterium. Continuously, after solid matters in the waste water which is treated by yeast are digested and BOD is lowered, the resulting waste water is treated by activated sludge. As the yeast, yeast having high decomposing function belonging to Hanzenura genus or other genuses is employed and as the yeast-lysing bacterium, yeast-lysing bacterium YLM-1 (gram negative bacterium) is preferable. Organic waste water, for example, is introduced to an yeast tank from a raw water tank by a pump P and brought into contact with yeast to be purified. At that time, the waste water in the tank is stirred and a blower B is driven to carry out ventilation and the pH in the tank is kept optimum.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a wastewater treatment method, and more particularly, to a novel wastewater capable of efficiently treating high-concentration wastewater and significantly reducing the amount of excess sludge produced. It relates to a processing method.

[0002]

2. Description of the Related Art As a method for treating organic wastewater, biological methods, chemical methods, physical or mechanical methods have been conventionally known. For example, food manufacturing wastewater, brewing wastewater, agricultural production wastewater, etc. A biological method such as an activated sludge method is frequently used for the treatment of sewage. However, these known methods are not yet fully satisfactory.

[0003]

[Problems to be Solved by the Invention] From the viewpoint of pollution prevention such as river pollution prevention, in particular, regulations on wastewater have been strengthened in recent years, and establishment of an efficient and excellent wastewater treatment method is strongly desired in the industry. ing.

On the other hand, in biological treatment such as activated sludge method, a large amount of sludge is produced as a byproduct after wastewater treatment, and secondary pollution occurs unless not only wastewater itself but also byproduct sludge is not efficiently treated. For the first time, the present inventors also paid attention to the risk.

The present invention has been made for the purpose of solving these problems all at once.

[0006]

[Means for Solving the Problems] As a result of studies from various fields to achieve the above object, in order to efficiently treat high-concentration wastewater, a method of treating with yeast before directly treating with activated sludge or We first focused on the need for a method of treating yeast alone.

As yeasts, all yeasts can be used alone or in combination of two or more, as long as they are yeasts having a high ability to decompose pollutants in wastewater. The yeast that can be used is, for example,
Hansenula yeasts such as Hansenula anorama, and Kluyveromyces yeasts such as Kluyveromyces marxianus, K. et al. Lactis, Candida yeast, and Trichosporon yeast.

Examples of representative strains include the following: Hansenula an
omala) J-224 (FERMP-7671), J622, J-45-0, H. fabianii J-640: Kluyveromyces marxianus (Kluyveromyces)
marxianus) J-673 (FERM P-10
385): Candida Species
sp. ) (FERM P-3965): Trichosporon species
p. ) AN-161.

Although the organic waste water is sufficiently treated by treating with yeast in this way, if it is treated to a higher degree or contains components that cannot be treated sufficiently with yeast, activated sludge treatment is required. It may be used together. The activated sludge treatment can be appropriately performed according to a conventional method.

However, in the present invention, when the wastewater is purified by yeast treatment and further treated with yeast-dissolving bacteria, the grown yeast is not only dissolved, but also the digestion of solids in the wastewater is significantly reduced. It was promoted, the BOD value was greatly reduced, and useful new knowledge was obtained that more advanced wastewater treatment is performed. It was also confirmed that the combined use of yeast lysing treatment can significantly suppress the generation of excess sludge and prevent the generation of secondary pollution due to sludge.
Moreover, it was confirmed that the yeast lysate treatment had no adverse effect on the activated sludge treatment, and based on these useful new findings, an efficient integrated drainage system was constructed.

In the present invention, any microorganism can be used as long as it can dissolve yeast. For example, yeast lysing bacterium YLM-1 (named Rarobacter faecitabidus) is suitable as the microorganism used in the present invention. It is one of the things.

YLM-1 is a relatively small Gram-negative bacillus having the following properties, and no spores were observed. (1) Cell shape Bacillus (2) Cell size 0.3 to 0.4 ×
0.9-1.8 μm (3) Presence / absence of cell polymorphism None (4) Motile (5) Flagellation status of polar flagella (6) Gram stain positive (7) Acid-positive (8) Yeast (9) Growth pH 7-9 (growth optimum pH 7) (10) Growth optimum temperature 30-35 ° C

The separation method of YLM-1 is as follows:
YNB (East Nitrogen Base, Difco
1% agar and Hansenula anoma yeast
laJ-45-0 is added at about 2 × 10 ⁷ cells / ml. The YNB agar medium is sterilized in an autoclave, cooled to about 50 ° C., and then live yeast cells are added. The separated source sample is appropriately diluted, inoculated at the same time as yeast, mixed, poured into a petri dish, and cultured. YLM-1 bacteria were isolated by culturing at 35 ° C. for 5 to 10 days and deposited (FERM P-6781).

When YLM-1 was stab-cultured on a viable yeast agar medium, lysis of yeast was observed from the surface of the medium at 30 ° C. for 3 days. About one week later, the yeast in the whole medium in a test tube was cultured. Lysed. In addition, YLM-1 was inoculated on a yeast agar medium, solidified in a test tube, and cultured at 30 ° C. As a result, colonies and lytic spots were detected on the entire surface in one week. The lysis spectrum is as shown in Tables 1 and 2 below.

[0015]

[Table 1]

[0016]

[Table 2]

As is clear from the above results, YLM-1
After treating the organic wastewater with the various yeasts listed in these tables, the used yeast can be efficiently lysed. As described above, the yeast lysate not only lyses the yeast but also has another excellent wastewater treatment effect.

Yeast lysates include YLM-1 described above.
In addition to bacteria, any microorganism can be used as long as it is a microorganism capable of lysing yeast, and examples thereof include Oerskovia turbata (IF).
O 13506), Oerskovia xanthineolyt
ica), Oerskofia Citria (Oersko)
via city, FERM P-2003), Oerskophia species YS-016 (Oer
scovia sp. YS-016, FERM P-1
1737) and the like can be used.

Oerskophia citria produces a yeast cell wall lysing enzyme and is characterized by excellent yeast lytic properties, and exhibits the following mycological properties. (1) Morphology: Hyphae are formed in the early stage of culture and then (about 24
(Time) A bacterial cell becomes a bacterial cell by rupture, and the average size of the bacterial cell is 0.4 to 0.6 × 1.0 to 3.0 μm, and both ends are tinged. In old culture (after 72 hours), it changes to spherical or amorphous and the average size is 0.8 to 1.0.
× 0.8 to 1.0 μ. (2) Aerial mycelium: Does not form. (3) Motility: Bacterial cells that are observed due to rupture of hyphae have motility. (4) Endospores: do not form. (5) Flagella: 1-3 side hairs grow on each bacterial cell. (6) Gram stainability: Positive (7) Anti-acidity: None (8) Yeast solubility: Yes

Oerskophia YS-016 can dissolve ascomycete yeast, basidiomycete yeast and imperfect fungal yeast, has a broad lytic spectrum, and exhibits the following mycological properties. (1) Morphology: Mycelium is exhibited from the early stage to the middle stage of culture,
After that, due to rupture, it splits from short rod-shaped to coccoid. The average size is 0.3 to 0.6 × 1.3 to 3.0 ≦. (2) Aerial hyphae: None (3) Motility: Yes (4) Presence or absence of spores: None (5) Flagella: Extreme flagella (6) Gram stainability: Positive (7) Anti-acidity: None (8) Yeast lysis Gender: Yes

The same applies to yeasts, but yeast lysates can be used alone or in combination of two or more kinds.
Killed cells, cultures, and / or processed products can be used. As the processed product, a concentrate of the culture obtained by culturing yeast lysate, a paste, a dried product, a diluted product, a solid obtained by solid-liquid separation, and / or various cultures such as the liquid part All processed products are included.

As described above, according to the present invention, the waste water is treated with yeast, and then with yeast lysing bacteria, and if necessary, activated sludge treatment is performed (the yeast lysing treatment and the activated sludge treatment are simultaneously performed in parallel. The waste water can be efficiently purified. For example, as shown in the treatment flow shown in Table 3 below, yeast having a high degree of degrading pollutants was added to the wastewater to purify it, and then, along with the purification, the yeast that had proliferated was subjected to a lysing treatment with a yeast lysing bacterium to perform yeast treatment. Digests solids in wastewater, and further B
After reducing OD, combine with low-concentration wastewater if necessary,
Then, it may be treated with activated sludge.

[0023]

[Table 3]

FIG. 1 shows an example embodying such a processing flow. FIG. 1 is an embodiment of an apparatus for carrying out the wastewater treatment method according to the present invention, the operation of which is as follows. First, the organic waste water is introduced into the yeast tank from the raw water tank by the pump P. The organic wastewater is thoroughly contacted with the yeast in the yeast tank to purify the wastewater. If necessary, the inside of the tank is stirred and blower B is operated to ventilate. Also,
In the yeast tank, the pH in the tank is measured by a pH controller, and when leaning toward the alkali side, an acid such as sulfuric acid is introduced into the tank by the pump P to maintain the optimum pH. Furthermore, if desired, an adjusting tank (not shown) may be provided separately from the yeast tank to control pH, control wastewater concentration and the like.

In this manner, yeasts belonging to Hansenula and Candida are treated with various wastewater (washed wastewater (pH 4.
0): A, clarifier water from a starch manufacturing plant: B, malt whiskey distilling waste liquid: C, dilute sake (10 times): D), the growth yield when aerated and stirred is as shown in Table 4 below. Is.

[0026]

[Table 4]

The effluent purified by the yeast treatment while preventing the yeast lysing bacteria from being mixed is introduced into the digestion tank. In the digestion tank, as in the case of the yeast tank, stirring and aeration are performed, not only the yeast is lysed by the action of yeast lysing bacteria (YLM-1, etc.), but also the solid content in the waste water is digested. BOD is also reduced. Table 5 below shows the growth yield when Hansenula yeast was used as the substrate and YLM-1 was used as the yeast lysate. In this case, since TOC is reduced due to the endogenous respiration of the yeast even if only the yeast cells are aerated and agitated, the growth yield of YLM-1 is reduced to the TO of the first yeast.
T based on C and after shaking culture of yeast alone
It was calculated as an average value with OC as a reference.

[0028]

[Table 5]

After treatment in the digestion tank, it may be directly treated in the activated sludge tank, but after once adding low-concentration wastewater in the adjusting tank or adjusting the pH and the concentration. The activated sludge may be treated.

The activated sludge treatment may be carried out according to a conventional method. For example, the wastewater is introduced into the activated sludge tank from the adjusting tank by the pump P and is sufficiently contacted with the activated sludge while being aerated from the blower B to treat the wastewater and also to accumulate and proliferate the yeast-dissolving bacteria. After the activated sludge treatment, it is transferred to a settling tank to separate the solid and liquid, and the liquid portion is transferred to the treated water tank or discharged to a river or the like, while the solid portion is partially returned to the activated sludge tank as return sludge. In this way, not only batch processing but also continuous processing became possible.

In the present invention, various organic wastewaters are efficiently treated. For example, wastewater for sake production such as rice washing wastewater; various shochu distillation waste liquids; whiskey, brandy distillation waste liquids;
Distillers solubles; various agricultural production waste liquids such as starch production waste liquids; kitchen wastewater; and various other organic wastewaters can be treated extensively. Examples of the present invention will be described below.

[0032]

Example 1 A filtrate obtained by filtering a barley shochu distilled lees having the properties shown in Table 6 below using the apparatus shown in FIG. 1 (barley shochu distilled lees dehydrated filtrate: its properties are shown in Table 7 below). Was processed.

[0033]

[Table 6]

[0034]

[Table 7]

The yeast treatment, digestion treatment and activated sludge treatment were carried out under the conditions shown in Table 8 below. Hans as yeast
Enula anomala J-45-0 was used, and YLM-1 (FERM P-678 was used as a yeast lysate.
1) was used.

[0036]

[Table 8]

The apparatus was operated for 60 days, and the BOD of treated water and the solid content of the treated water in the digestion tank were measured over time to obtain the results shown in FIGS. 2 and 3, respectively.

The properties of yeast treated water and digested treated water were also measured, and the results shown in Table 9 below were obtained. Further, the results of Table 10 and Table 11 below were obtained for the influence of volume load on the yeast treatment and the solubilization of the solid content by the digestion treatment, respectively.

[0039]

[Table 9]

[0040]

[Table 10]

[0041]

[Table 11]

[0042]

EFFECTS OF THE INVENTION According to the present invention, by adopting a novel constitution in which yeast treatment and yeast lysis treatment are combined, not only various organic wastewaters can be efficiently treated, but also proliferated yeast is lysed. By doing so, generation of excess sludge can be suppressed, sludge treatment can be significantly reduced, and secondary pollution caused by sludge can be suppressed or prevented in advance.

The yeast lysing treatment not only lyses the used yeast, but also further digests the solids in the wastewater and reduces the BOD, resulting in an unexpected new effect, which is very efficient. Wastewater treatment is possible.

[Brief description of drawings]

FIG. 1 shows an embodiment of an apparatus for carrying out wastewater treatment according to the present invention.

FIG. 2 shows the daily change of treated water BOD in a yeast tank and a digester tank.

FIG. 3 shows daily changes in solid content in a yeast tank and a digester tank.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Jin Shimohan 2-6-30 Takinogawa, Kita-ku, Tokyo Inside the National Tax Agency Brewing Laboratory (72) Inventor Makoto Tatenuma 2-6-30 Takinogawa, Kita-ku, Tokyo National Tax Agency Brewing Test In-house (72) Inventor Osamu Suzuki 1443 Nakajuku, Annaka-shi, Gunma TDI Biotechnology Laboratory

Claims (7)

[Claims] 1. A method for treating wastewater, which comprises treating the wastewater with yeast and yeast-lysing bacteria. 2. In the wastewater treated with yeast, the pollutants in the wastewater are purified by using yeast having a high decomposing ability, and then the yeast that has grown in association with the purification is subjected to a lysing treatment with yeast-dissolving bacteria. The method for treating waste water, which comprises digesting the solid content of, and further reducing BOD, and then treating with activated sludge. 3. The method according to claim 1 or 2, wherein after the bacteriolysis treatment, low-concentration wastewater is added to perform activated sludge treatment. 4. A high-resolution yeast belonging to the genera Hansenula, Kluyveromyces, Candida, and / or Trichosporone is used as yeast. The method according to item 1. 5. The yeast lysate used is a live bacterium, a dead bacterium, a culture, and / or a treated product of the yeast lysate, which is used in any one of claims 1 to 4. Or 1
The method described in the section. 6. A treated product of a yeast lysate culture is a concentrate, a paste, a dried product, a diluted product, a solid product obtained by solid-liquid separation of a culture obtained by culturing a yeast lysate, and The method according to claim 5, wherein the method is the same liquid material. 7. The method according to any one of claims 1 to 6, wherein YLM-1 having the following mycological properties is used as a yeast lysing bacterium. (1) Cell shape Bacillus (2) Cell size 0.3 to 0.4 ×
0.9-1.8 μm (3) Presence / absence of cell polymorphism None (4) Motile (5) Flagellation status of polar flagella (6) Gram stain positive (7) Acid-positive (8) Yeast Has lytic properties