JPH0819385A - Method for extracting essence of fish, shellfish and alga containing large quantity of mineral component - Google Patents
Method for extracting essence of fish, shellfish and alga containing large quantity of mineral componentInfo
- Publication number
- JPH0819385A JPH0819385A JP6155598A JP15559894A JPH0819385A JP H0819385 A JPH0819385 A JP H0819385A JP 6155598 A JP6155598 A JP 6155598A JP 15559894 A JP15559894 A JP 15559894A JP H0819385 A JPH0819385 A JP H0819385A
- Authority
- JP
- Japan
- Prior art keywords
- extract
- fish
- shellfish
- algae
- mineral components
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Meat, Egg Or Seafood Products (AREA)
Abstract
Description
【0001】[0001]
【産業上の利用分野】本発明は、医薬品、機能性食品、
薬剤、各種成長剤、家畜の飼料添加剤、調味料等に有用
であり、原料魚貝藻類に含まれるリン、カルシウム、亜
鉛、鉄、カリウム等のミネラル成分及びタウリンが多く
含有される多量ミネラル成分含有魚貝藻類エキスの抽出
方法に関する。BACKGROUND OF THE INVENTION The present invention relates to pharmaceuticals, functional foods,
It is useful for medicines, various growth agents, feed additives for livestock, seasonings, etc., and minerals such as phosphorus, calcium, zinc, iron, potassium contained in raw material fish and shellfish algae, and a large amount of taurine-rich minerals. The present invention relates to a method for extracting contained fish and shellfish algae extracts.
【0002】[0002]
【従来の技術】従来魚貝藻類に蛋白分解酵素を作用さ
せ、エキス分と廃物とに分離して、魚貝藻類エキスを抽
出する方法が種々提案されている。得られる魚貝藻類エ
キスは、有効なペプチドや遊離アミノ酸を含有してお
り、各種機能性食品添加物、薬剤、成長剤、家畜の飼料
添加剤、調味料等に利用されている。具体的には例えば
特公昭53−31935号公報には、50〜60℃、p
H9〜10において耐アルカリ性蛋白分解酵素を原料魚
に添加して蛋白質を分解した後、pHを5〜6に調整し
て耐酸性蛋白分解酵素を添加・分解し、その分離液を濃
縮する方法が、特公平1−14885号公報には、原料
魚貝類に、枯草菌産生蛋白分解酵素と、麹菌産生蛋白分
解酵素とをpH6.0〜7.0において、2段階で作用
させ実質的に分子量3000以下のペプタイドアミノ酸
群及び遊離アミノ酸に分解し、その分離液を分離濃縮し
て薬理作用を有する魚貝類エキスを製造する方法が、特
公平5−73731号公報には、蛋白分解酵素により魚
類を分解して得た分子量3500〜15000のペプチ
ドを含む蛋白分解エキスを有効成分として含有する免疫
賦活剤が提案されている。この他に、原料魚貝類を耐酸
性蛋白分解酵素により1段階で処理して有効エキスを抽
出する方法、これらの得られたエキスを調味料、成長剤
等の用途に利用する方法等が提案されている。2. Description of the Related Art Conventionally, various methods have been proposed in which a proteolytic enzyme is allowed to act on fish and shellfish algae to separate it into an extract and wastes to extract the fish and shellfish algae extract. The resulting fish and shellfish algae extract contains effective peptides and free amino acids, and is used as various functional food additives, drugs, growth agents, livestock feed additives, seasonings and the like. Specifically, for example, JP-B-53-31935 discloses that the temperature is 50 to 60 ° C., p
In H9-10, a method of adding an alkali-resistant protease to the raw fish to decompose the protein, adjusting the pH to 5-6, adding / decomposing the acid-resistant protease, and concentrating the separated liquid Japanese Patent Publication No. 14885/1989 discloses that raw fish and shellfish are allowed to act in a two-step manner with a Bacillus subtilis-produced protease and an Aspergillus-produced protease at a molecular weight of 3000. The method for producing a fish and shellfish extract having a pharmacological action by decomposing the following peptide amino acid groups and free amino acids and separating and concentrating the separated liquid is disclosed in Japanese Examined Patent Publication No. 5-73731 and decomposes fish with a proteolytic enzyme. An immunostimulant containing a proteolytic extract containing the thus obtained peptide having a molecular weight of 3500 to 15000 as an active ingredient has been proposed. In addition, a method of treating raw fish and shellfish with an acid-resistant proteolytic enzyme in one step to extract an effective extract, a method of using the obtained extract for a seasoning, a growth agent, etc. are proposed. ing.
【0003】またこれら従来の方法では、原料魚貝類等
の蛋白質を分解して、用途に応じた有効ペプチド等を抽
出することを目的としており、その精製工程は、例えば
遠心分離法、真空分離法等により蛋白分解酵素で分解し
たエキス分と、廃物とを分離する方法が採用されてお
り、エキス分を薬剤等に利用する場合には、前記分離さ
れたエキス分に対して更にイオン交換膜等を利用した精
製工程を施して純度の高い有効成分の抽出が行なわれて
いる。Further, these conventional methods are aimed at decomposing proteins such as raw fish and shellfish to extract effective peptides and the like according to the intended use, and the purification step thereof is, for example, a centrifugal separation method or a vacuum separation method. A method of separating the extract component decomposed by a proteolytic enzyme and the wastes is adopted, and when the extract component is used as a drug or the like, an ion exchange membrane or the like is added to the separated extract component. The active ingredient with high purity is extracted by carrying out a purification process utilizing
【0004】ところで、最近ミネラル成分のヒト又は家
畜等に対する影響が重要視されており、特に亜鉛成分
は、アルカリフォスファターゼ、アルコールデヒドロキ
ナーゼ、脱炭酸酵素、カルボキシペプチターゼA、ウリ
カーゼ、DNAポリメラーゼ、RNAポリメラーゼ等の
亜鉛酵素の構成成分、活性中心として働く他、有機性の
リガンドを形成して酵素を活性化する働きもあり重要な
役割を担っていることが提案されている。そこで現在、
前記エキス分を例えば機能性食品、家畜の飼料添加剤等
に利用する場合には、リン、カルシウム、亜鉛等の新た
なミネラル成分を添加して成分調整した後、最終製品化
されている。By the way, recently, the influence of mineral components on humans or livestock has been emphasized. Particularly, zinc components are alkaline phosphatase, alcohol dehydrokinase, decarboxylase, carboxypeptidase A, uricase, DNA polymerase, RNA polymerase. It has been proposed that it plays an important role in that it functions as a constituent component of zinc enzymes such as the above, as an active center, and also functions as forming an organic ligand to activate the enzyme. So now,
When the extract is used, for example, as a functional food or a feed additive for livestock, it is finalized after adding new mineral components such as phosphorus, calcium and zinc to adjust the components.
【0005】一方、前記エキス分を抽出する原料魚貝藻
類中には、種々のミネラル成分が含有されていることが
知られている。しかしながら、前述の従来の有効エキス
の抽出方法では、用途に応じた有効ペプチド等を含むエ
キスの抽出が目的とされているため、原料に含有される
ミネラル成分の殆どは、分離された廃物に含有された状
態で廃棄されているのが現状である。On the other hand, it is known that various mineral components are contained in the raw fish, shellfish and algae from which the extract is extracted. However, in the above-mentioned conventional method for extracting an effective extract, since the purpose is to extract an extract containing an effective peptide or the like according to the purpose, most of the mineral components contained in the raw material are contained in the separated waste. The current situation is that they are discarded in the state of being discarded.
【0006】そこで、原料魚貝藻類に含有されるミネラ
ル成分を有効に利用する方法として、前述のエキスと廃
物との分離工程の前に、ミネラル成分の精製工程を行な
ったり、また分離された廃物からミネラル成分を精製し
てエキス分に添加する方法が考えられるが、このような
精製工程の追加は、方法の煩雑化を招き、更にコスト的
にも分離されたエキス分に新たなミネラル成分を添加す
る現在の方法よりも高価となり、工業的生産には不向き
であるという欠点がある。Therefore, as a method of effectively utilizing the mineral components contained in the raw material fish and shellfish algae, a purification process of the mineral components is carried out before the separation process of the above-mentioned extract and the waste products, or the separated waste products. It is possible to purify the mineral component from the extract and add it to the extract, but the addition of such a purification step complicates the method, and a new mineral component is added to the separated extract in terms of cost. It has the disadvantage of being more expensive than the current method of addition and unsuitable for industrial production.
【0007】[0007]
【発明が解決しようとする課題】従って本発明の目的
は、魚貝藻類中に含まれるミネラル成分と、有効なペプ
チド成分とを多量に含むエキスを、容易で、且つ簡便な
方法で抽出することができる多量ミネラル成分含有魚貝
藻類エキスの抽出方法を提供することにある。Therefore, an object of the present invention is to extract an extract containing a large amount of a mineral component contained in fish and shellfish algae and an effective peptide component by an easy and simple method. An object of the present invention is to provide a method of extracting a fish and shellfish alga extract containing a large amount of mineral components.
【0008】[0008]
【課題を解決するための手段】本発明によれば、原料魚
貝藻類を蛋白分解酵素により蛋白分解し、エキス分と廃
物とに精製分離して魚貝藻類エキスを抽出する方法にお
いて、耐酸性蛋白分解酵素を作用させて蛋白分解する際
のpHを5.0未満とし、前記エキス分中にミネラル成
分を溶出させることを特徴とする多量ミネラル成分含有
魚貝藻類エキスの抽出方法が提供される。According to the present invention, a method of proteolytically decomposing raw material fish and shellfish algae with a proteolytic enzyme and purifying and separating into extract and waste to extract a fish and shellfish algae extract Provided is a method for extracting a large amount of mineral component-containing fish and shellfish alga extract, which is characterized in that a pH during proteolysis by acting a proteolytic enzyme is set to less than 5.0 and a mineral component is eluted in the extract. .
【0009】以下本発明を更に詳細に説明する。本発明
の抽出方法では、まず原料魚貝藻類を蛋白分解酵素によ
り蛋白質を分解するが、この際耐酸性蛋白分解酵素によ
る分解を、特定pHにおいて行なう。The present invention will be described in more detail below. In the extraction method of the present invention, the raw material fish and shellfish algae are first subjected to protein degradation with a protease, and at this time, the degradation with an acid-resistant protease is carried out at a specific pH.
【0010】前記原料魚貝藻類としては、例えばアジ、
サバ、イワシ、サンマ、カツオ、ホッケ、タラ、イカ、
タコ、エビ、カキ、シジミ、アサリ、イガイ、モガイ、
アカガイ、ハマグリ、ワカメ、コンブ、ヒジキ又はこれ
らの混合物等を挙げることができる。また蛋白分解酵素
としては、蛋白質を分解することができるものであれば
特に限定されるものではなく、得られる有効エキス分の
用途や蛋白分解の条件等に応じて種々選択することがで
きる。具体的には枯草菌産生蛋白分解酵素、麹菌産生蛋
白分解酵素又はこれらの混合物等を挙げることができ
る。このように蛋白分解酵素としては、従来原料魚貝藻
類を蛋白分解酵素により蛋白分解し、エキス分と廃物と
に精製分離して魚貝藻類エキスを抽出する方法において
用いられている公知のものを使用することができるが、
蛋白分解酵素を1段階で作用させる場合には、耐酸性蛋
白分解酵素を用いる必要があり、また複数段階で蛋白分
解酵素を作用させる場合には、少なくとも1回は、好ま
しくは最終段階において耐酸性蛋白分解酵素を用いる必
要がある。該耐酸性蛋白分解酵素の使用は、原料魚貝藻
類の蛋白質を各種用途に応じたペプチドの分子量まで分
解するためである。Examples of the raw material fish and shellfish algae include horse mackerel,
Mackerel, sardines, saury, bonito, hockey, cod, squid,
Octopus, shrimp, oyster, blue clam, clam, mussel, mussel,
Examples thereof include red mussel, clam, seaweed, kelp, hijiki or a mixture thereof. The proteolytic enzyme is not particularly limited as long as it can decompose a protein, and various kinds can be selected depending on the use of the obtained effective extract, the conditions for proteolysis and the like. Specific examples thereof include Bacillus subtilis-produced protease, Aspergillus-produced protease, or a mixture thereof. As described above, as the proteolytic enzyme, a known one used in the method of extracting a fish and shell alga extract by proteolytically decomposing the raw material fish and shell alga with a proteolytic enzyme and purifying and separating it into an extract and a waste product is used. Can be used,
When the proteolytic enzyme acts in one step, it is necessary to use an acid-resistant proteolytic enzyme, and when the proteolytic enzyme acts in multiple steps, the acid-resistant protease should be used at least once, preferably in the final step. It is necessary to use proteolytic enzymes. The use of the acid-resistant proteolytic enzyme is for degrading the protein of the raw material fish and shellfish alga to the molecular weight of the peptide according to various uses.
【0011】前記原料魚貝藻類を蛋白分解するには、例
えばまず細切り・スラリー化等の前処理を施した原料魚
貝藻類、魚貝類の内臓等の一部を取り出した魚貝類の部
分若しくは丸ままの魚貝藻類を反応缶に投入する。投入
後好ましくは、直ちに75℃以上、特に好ましくは80
℃以上に昇温して魚貝藻類の中に含まれる自己分解酵素
を失活させて魚貝藻類特有の生くさみ、悪臭等の臭気を
除去するのが望ましい。次いで、温度40〜70℃、p
H5.0〜7.0、好ましくはpH5.5〜6.5にお
いて、耐アルカリ性蛋白分解酵素を添加して、魚貝藻類
に含まれる蛋白質を粗分解する。この耐アルカリ性蛋白
分解酵素による粗分解は必ずしも行なう必要はなく、耐
酸性蛋白分解酵素1段階により有効エキス成分までの分
解条件を緩和するための工程である。この際分解時間は
臨界的なものではないが、通常30分〜10時間、好ま
しくは1〜6時間行う。In order to proteolytically decompose the above-mentioned raw fish and shellfish algae, for example, the raw fish and shellfish algae which have been subjected to pretreatment such as shredding and slurrying, part of the internal organs of the fish and shellfish, or the portion of the fish shellfish or the whole Put whole fish and shellfish algae into the reaction can. Immediately after the addition, the temperature is preferably 75 ° C. or higher, particularly preferably 80.
It is desirable to raise the temperature to ℃ or more to deactivate the autolytic enzyme contained in the fish and shellfish algae to remove the raw shavings, malodors and the like peculiar to the fish and shellfish algae. Then, the temperature is 40 to 70 ° C., p
At H5.0 to 7.0, preferably pH 5.5 to 6.5, an alkali-resistant protease is added to roughly decompose proteins contained in fish and shellfish algae. It is not always necessary to perform the crude decomposition with the alkali-resistant protease, but it is a step for relaxing the decomposition conditions up to the effective extract component by one step of the acid-resistant protease. At this time, the decomposition time is not critical, but is usually 30 minutes to 10 hours, preferably 1 to 6 hours.
【0012】前記耐アルカリ性蛋白分解酵素による粗分
解を行なった場合には、温度を少なくとも75℃以上、
好ましくは80℃以上に昇温し、耐アルカリ性蛋白分解
酵素を完全に不活性化させるのが望ましい。不活性化時
間は通常10分〜1時間、好ましくは15〜30分であ
る。この不活性化工程を行なわない場合には、後述する
必須工程である耐酸性蛋白分解酵素による蛋白質分解工
程において、前段階の酵素が併存して作用し、目的とす
る有効ペプチドへの分解が困難になるので好ましくな
い。In the case of performing the crude decomposition with the alkali-resistant protease, the temperature is at least 75 ° C. or higher,
It is preferable to raise the temperature to 80 ° C. or higher to completely inactivate the alkali-resistant protease. The inactivation time is usually 10 minutes to 1 hour, preferably 15 to 30 minutes. If this inactivation step is not carried out, in the proteolytic step by an acid-resistant proteolytic enzyme, which is an essential step to be described later, the enzyme in the previous step acts together to make it difficult to decompose into the target effective peptide. Is not desirable.
【0013】前記自己分解酵素を失活させた原料魚貝藻
類又は耐アルカリ性蛋白分解酵素による粗分解物に、好
ましくは30〜60℃において、耐酸性蛋白分解酵素を
添加して蛋白分解する。この際pHは5.0未満、好ま
しくはpH4.5以下、特に好ましくはpH4.0未満
とする。pHが5.0以上の場合には、原料魚貝藻類中
に含有されるリン、カルシウム、亜鉛等のミネラル成分
及びタウリンが有効ペプチドを含むエキス分側に溶出せ
ず、最終的に得られる魚貝藻類エキス中のミネラル成分
含有量が極端に減少する。一方pHの下限は、pHが低
いほどミネラル成分のエキス分への溶出量が増大するの
で特に制限されるものではないが、操作性等の点から高
温度で処理する場合にはpH1.0以上が好ましく、4
0℃以下の低温度で処理する場合にはpH2.0以上が
好ましい。この耐酸性蛋白分解酵素による蛋白分解の分
解時間は、この分解に供する原料成分、耐酸性蛋白分解
酵素の種類、目的とする有効ペプチドの分子量等により
異なるが、通常30分〜5時間程度で行なうことができ
る。The acid-degrading protease is added to the crude fish-and-sea alga in which the autolytic enzyme is inactivated or the crude decomposed product by the alkali-resistant protease, preferably at 30 to 60 ° C., for protein degradation. At this time, the pH is less than 5.0, preferably 4.5 or less, and particularly preferably less than 4.0. When the pH is 5.0 or higher, the mineral components such as phosphorus, calcium, zinc and taurine contained in the raw material shellfish algae do not elute on the side of the extract containing the effective peptide, and the fish finally obtained. The content of mineral components in shellfish algae extract is extremely reduced. On the other hand, the lower limit of pH is not particularly limited because the lower the pH, the more the elution amount of the mineral component into the extract increases, but from the viewpoint of operability, the pH is 1.0 or more. Is preferred and 4
When treating at a low temperature of 0 ° C. or lower, pH of 2.0 or higher is preferable. The proteolytic degradation time by the acid-resistant protease is usually about 30 minutes to 5 hours, although it varies depending on the raw material components to be used for the decomposition, the type of acid-resistant protease, the molecular weight of the target effective peptide, and the like. be able to.
【0014】前記特定pH条件において、耐酸性蛋白分
解酵素による処理を行なうことにより、原料魚貝藻類の
蛋白質が、所望用途に応じた有効ペプチド成分にまで分
解され、該有効ペプチドを含むエキス分中に、原料魚貝
藻類に含有されているミネラル成分が多量溶出する。該
耐酸性蛋白分解酵素による処理の後、好ましくは直ちに
75℃以上、特に好ましくは80℃以上に昇温し、耐酸
性蛋白分解酵素を完全に不活性化するのが望ましい。By performing treatment with an acid-resistant proteolytic enzyme under the above-mentioned specific pH conditions, the protein of the raw material fish and shellfish algae is decomposed into effective peptide components according to the desired use, and the extract containing the effective peptide is decomposed. In addition, a large amount of mineral components contained in the raw fish, shellfish and algae are eluted. After the treatment with the acid-resistant protease, the temperature is preferably raised to 75 ° C. or higher, particularly preferably 80 ° C. or higher to completely inactivate the acid-resistant protease.
【0015】本発明の抽出方法では、前記耐酸性蛋白分
解酵素による処理の後、エキス分と廃物とに精製分離し
て目的の多量ミネラル成分含有魚貝藻類エキスを得るこ
とができる。In the extraction method of the present invention, after the treatment with the acid-resistant proteolytic enzyme, the extract component and the waste product are purified and separated to obtain the desired algae extract containing a large amount of mineral components.
【0016】前記エキス分と廃物との精製分離は、例え
ば公知の真空濾過法、遠心分離法等に従い常法にて行な
うことができる。分離されたエキス分は、所望用途に応
じた有効ペプチド類;イソロイシン、ロイシン、リジ
ン、メチオニン、フェニールアラニン、スレオニン、ト
リプトファン、バリン等の遊離アミノ酸類;タウリン及
びカリウム、リン、マグネシウム、カルシウム、亜鉛、
鉄、モリブデン、アルミニウム、マンガン、銅、リチウ
ム、スズ、ニッケル等の原料魚貝藻類に応じたミネラル
成分を主成分として含む水分量90重量%以上の成分で
あり、廃物は、油層及び骨片類等の未分解物である。Purification and separation of the extract and the waste can be carried out by a conventional method, for example, according to a known vacuum filtration method, centrifugal separation method or the like. The separated extract is an effective peptide according to the desired application; isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, free amino acids such as valine; taurine and potassium, phosphorus, magnesium, calcium, zinc,
Iron, molybdenum, aluminum, manganese, copper, lithium, tin, nickel, etc. A raw material is a component having a water content of 90% by weight or more, which contains a mineral component according to the raw fish, shellfish and algae as the main component, and the waste is an oil layer and bone fragments. It is an undecomposed material such as.
【0017】得られたエキス分は、そのまま目的とする
多量ミネラル成分含有魚貝藻類エキスとすることができ
る他、前記エキス分中にミネラル成分を溶出させた後、
pHを上げる方法等により目的とする多量ミネラル成分
含有魚貝藻類エキスを得ることができる。前記pHは、
原料魚貝藻類のもとのpHまで上げるのが好ましく、具
体的にはpH5.5〜7.0にするのが望ましい。更に
必要に応じ精製法を用いて有機ミネラルを抽出すること
もできる。またミネラル成分含有量を維持した状態で、
更に有効ペプチド類を高純度化するために、イオン交換
樹脂膜等を利用した精製を常法に従って行なうこともで
きる他、好ましくは60℃以下において水分量30〜6
0%程度に減圧濃縮する方法等により、目的とする魚貝
藻類エキスとすることができる。The extract thus obtained can be used as it is as a target fish and shellfish alga extract containing a large amount of mineral components, and after the mineral components are eluted into the extract,
The target fish and shellfish alga extract containing a large amount of mineral components can be obtained by a method of increasing pH and the like. The pH is
It is preferable to raise the pH to the original pH of the raw fish, shellfish and algae, and specifically, it is desirable to adjust the pH to 5.5 to 7.0. If necessary, the organic mineral can be extracted by using a purification method. In addition, while maintaining the content of mineral components,
Further, in order to highly purify the effective peptides, purification using an ion exchange resin membrane or the like can be carried out by a conventional method, and preferably, the water content is 30 to 6 at 60 ° C. or lower.
The target fish and shell alga extract can be obtained by a method such as vacuum concentration to about 0%.
【0018】本発明の抽出方法により得られる魚貝藻類
エキスは、全て天然物のみからなっているため毒性はな
く、極めて安全な物質であり、医薬品、各種機能性食
品、薬剤、各種成長剤、家畜の飼料添加剤、調味料等の
用途に応じてその配合量等を適宜選択して利用すること
ができる。またミネラル成分が多く含有されているの
で、従来行なわれている新たなミネラル成分の添加を行
なう必要がないか、若しくは少量の添加で実施すること
ができる。The fish and shellfish alga extract obtained by the extraction method of the present invention is a non-toxic and extremely safe substance because it consists entirely of natural products, and is a drug, various functional foods, drugs, various growth agents, The blending amount and the like of the livestock feed additive, seasoning and the like can be appropriately selected and used. Further, since a large amount of mineral component is contained, it is not necessary to add a new mineral component which is conventionally used, or it can be carried out by adding a small amount.
【0019】[0019]
【発明の効果】本発明の魚貝藻類エキスの抽出方法で
は、耐酸性蛋白分解酵素を作用させて、蛋白分解する際
のpHを特定範囲とするという従来の工程における条件
を変えるのみで、原料魚貝藻類に含まれるミネラル成分
をエキス分に溶出させることができるので、工業的にも
利用可能な簡便な方法で、医薬品、各種機能性食品、薬
剤、各種成長剤、家畜の飼料添加剤、調味料等の用途に
応じた有効ペプチド等を含み、且つ原料魚貝藻類に含有
される多量のミネラル成分をも含有する魚貝藻類エキス
を得ることができる。INDUSTRIAL APPLICABILITY In the method for extracting a fish and shellfish alga extract of the present invention, the acid-resistant proteolytic enzyme is allowed to act, and the pH in the case of proteolysis is changed to a specific range by simply changing the condition in the conventional process. Since the mineral components contained in fish and shellfish algae can be eluted into the extract, it is a simple method that can be used industrially, such as pharmaceuticals, various functional foods, drugs, various growth agents, feed additives for livestock, It is possible to obtain a fish and shellfish alga extract containing an effective peptide or the like depending on the use such as a seasoning and also containing a large amount of mineral components contained in the raw fish and shellfish alga.
【0020】[0020]
【実施例】以下本発明を実施例及び比較例により更に詳
細に説明するが、本発明はこれらに限定されるものでは
ない。EXAMPLES The present invention will be described in more detail with reference to Examples and Comparative Examples, but the present invention is not limited thereto.
【0021】[0021]
【実施例1】カキ2kgを前処理を行わず、丸のまま、
10kgの水と共に撹拌機付き反応缶に入れ、80℃に
加熱した。15分後に50℃に温度を下げ、pH5.0
において市販の枯草菌産生蛋白分解酵素、商品名「アロ
アーゼAP−10」(ヤクルト生化学K.K.製)2g
を添加し、5時間反応させた。次いで80℃に昇温し、
15分間維持した後、50℃になるまで冷却し、クエン
酸を添加してpH4.0に調製した。これに麹菌産生蛋
白分解酵素、商品名「パンチターゼNP−2」(ヤクル
ト生化学K.K.製)2gを添加し、3時間反応させ
た。その後80℃に昇温して再び酵素を不活性化させ
た。この反応液を常法により遠心分離機でエキス層、油
層、骨片等未分解層に分離し、エキス層は濾過後60℃
において減圧濃縮して水分50%以下のカキエキスを調
製した。ミネラル成分、遊離アミノ酸成分及びペプタイ
ドアミノ酸群成分の割合を表1に示す。得られたエキス
成分中のミネラル成分の含有量を、エキス100g当た
りの量で示し、またタウリン、タンパク質、遊離アミノ
酸及びペプタイドアミノ酸群の含有量を、エキス成分全
量に対する重量%で示す。[Example 1] 2 kg of oysters were not pretreated and were left as they were,
It was put in a reaction can equipped with a stirrer together with 10 kg of water and heated to 80 ° C. After 15 minutes, lower the temperature to 50 ° C and adjust to pH 5.0.
Bacillus subtilis-produced proteolytic enzyme, trade name "Aroase AP-10" (manufactured by Yakult Biochemical KK) in Japan
Was added and reacted for 5 hours. Then raise the temperature to 80 ° C,
After maintaining for 15 minutes, the mixture was cooled to 50 ° C., and citric acid was added to adjust the pH to 4.0. 2 g of a koji mold-produced proteolytic enzyme, trade name "Punchtase NP-2" (manufactured by Yakult Biochemical KK) was added to this and reacted for 3 hours. Then, the temperature was raised to 80 ° C. to inactivate the enzyme again. This reaction solution is separated into an extract layer, an oil layer, an undecomposed layer such as bone fragments by a centrifuge by a conventional method, and the extract layer is filtered at 60 ° C.
Was concentrated under reduced pressure to prepare an oyster extract having a water content of 50% or less. Table 1 shows the ratios of the mineral component, the free amino acid component and the peptide amino acid group component. The content of the mineral component in the obtained extract component is shown by the amount per 100 g of the extract, and the contents of taurine, protein, free amino acid and peptide amino acid group are shown by weight% with respect to the total amount of the extract component.
【0022】[0022]
【実施例2】麹菌産生蛋白分解酵素を添加する際のpH
を2.0、商品名「パンチターゼNP−2」を商品名
「スミチームAP」(新日本化学工業(株)製)に代え
た以外は、実施例1と同様に行い水分50%以下のカキ
エキスを調製した。ミネラル成分、遊離アミノ酸成分及
びペプタイドアミノ酸群成分の割合を表1に示す。[Example 2] pH at the time of adding a protease produced by Aspergillus oryzae
Was changed to 2.0 and the trade name "Punchtase NP-2" was changed to the trade name "Sumiteam AP" (manufactured by Shin Nippon Chemical Industry Co., Ltd.), and oyster extract having a water content of 50% or less was prepared in the same manner as in Example 1. Prepared. Table 1 shows the proportions of mineral components, free amino acid components and peptide amino acid group components.
【0023】[0023]
【比較例1】麹菌産生蛋白分解酵素を添加する際のpH
を6.0に代えた以外は、実施例1と同様に行い水分5
0%以下のカキエキスを調製した。ミネラル成分、遊離
アミノ酸群及びペプタイドアミノ酸群成分の割合を表1
に示す。[Comparative Example 1] pH at the time of adding the protease of Aspergillus oryzae
The same procedure as in Example 1 was repeated, except that the water content was changed to 6.0.
An oyster extract of 0% or less was prepared. Table 1 shows the proportions of mineral components, free amino acids and peptide amino acids.
Shown in
【0024】[0024]
【表1】 [Table 1]
【0025】[0025]
【実施例3】実施例1で用いたカキと各成分の含有量が
異なるカキを用いた以外は、実施例1と同様にして行い
水分50%以下のカキエキスとした。ミネラル成分、遊
離アミノ酸群及びペプタイドアミノ酸群成分の割合を表
2に示す。Example 3 An oyster extract having a water content of 50% or less was prepared in the same manner as in Example 1 except that the oysters used in Example 1 were different from each other in the content of each component. Table 2 shows the proportions of the mineral component, the free amino acid group and the peptide amino acid group component.
【0026】[0026]
【実施例4】麹菌産生蛋白分解酵素を添加する際のpH
を3.0、商品名「パンチターゼNP−2」を商品名
「Acid protease A」(天野製薬(株)製)にし、濃縮
前にpHを6.0とした以外は、実施例3と同様にして
行い水分50%以下のカキエキスとした。ミネラル成
分、遊離アミノ酸成分及びペプタイドアミノ酸群成分の
割合を表2に示す。[Example 4] pH at the time of adding a protease produced by Aspergillus oryzae
In the same manner as in Example 3, except that the trade name "Punchtase NP-2" was changed to "Acid protease A" (manufactured by Amano Pharmaceutical Co., Ltd.) and the pH was adjusted to 6.0 before concentration. The oyster extract having a water content of 50% or less was obtained. Table 2 shows the ratios of the mineral component, the free amino acid component and the peptide amino acid group component.
【0027】[0027]
【比較例2】麹菌産生蛋白分解酵素を添加する際のpH
を5.0にした以外は、実施例3と同様にして行い水分
50%以下のカキエキスとした。ミネラル成分、遊離ア
ミノ酸群及びペプタイドアミノ酸群成分の割合を表2に
示す。[Comparative Example 2] pH at the time of adding a protease produced by Aspergillus oryzae
The oyster extract having a water content of 50% or less was obtained in the same manner as in Example 3 except that the oyster extract was changed to 5.0. Table 2 shows the proportions of the mineral component, the free amino acid group and the peptide amino acid group component.
【0028】[0028]
【表2】 [Table 2]
【0029】[0029]
【実施例5】実施例1で用いたカキと各成分の含有量が
異なるカキを用いた以外は、実施例1と同様にして行い
水分50%以下のカキエキスとした。ミネラル成分、遊
離アミノ酸成分及びペプタイドアミノ酸群成分の割合を
表3に示す。Example 5 An oyster extract having a water content of 50% or less was prepared in the same manner as in Example 1 except that the oysters used in Example 1 had different contents of each component. Table 3 shows the ratios of the mineral component, the free amino acid component and the peptide amino acid group component.
【0030】[0030]
【実施例6】麹菌産生蛋白分解酵素を添加する際のpH
を2.5、商品名「パンチターゼNP−2」を商品名
「精製パパイン」(アサヒビール社製)にし、濃縮後p
Hを7.0とした以外は、実施例4と同様にして行い水
分50%以下のカキエキスとした。ミネラル成分、遊離
アミノ酸群及びペプタイドアミノ酸群成分の割合を表3
に示す。[Example 6] pH at the time of adding Aspergillus oryzae-produced protease
2.5, the trade name "Punchatase NP-2" is changed to the trade name "Purified papain" (manufactured by Asahi Breweries), and after concentration p
An oyster extract having a water content of 50% or less was obtained in the same manner as in Example 4 except that H was 7.0. Table 3 shows the proportions of mineral components, free amino acids and peptide amino acids.
Shown in
【0031】[0031]
【比較例3】麹菌産生蛋白分解酵素を添加する際のpH
を6.0にした以外は、実施例4とと同様にして行い水
分50%以下のカキエキスとした。ミネラル成分、遊離
アミノ酸成分及びペプタイドアミノ酸群成分の割合を表
3に示す。[Comparative Example 3] pH at the time of adding the protease of Aspergillus oryzae
Was carried out in the same manner as in Example 4 except that the oyster extract had a water content of 50% or less. Table 3 shows the ratios of the mineral component, the free amino acid component and the peptide amino acid group component.
【0032】[0032]
【表3】 [Table 3]
【0033】[0033]
【実施例7】サバ2.5kgを前処理を行わず、丸のま
ま、12kgの水と共に撹拌機付き反応缶に入れ、80
℃に加熱した。15分後に50℃に温度を下げ、pH
8.0において市販の枯草菌産生蛋白分解酵素、商品名
「アロアーゼAP−10」(ヤクルト生化学K.K.
製)2gを添加し、5時間反応させた。次いで80℃に
昇温し、15分間維持した後、50℃になるまで冷却
し、酢酸を添加してpH4.0に調製した。これに麹菌
産生蛋白分解酵素、商品名「パンチターゼNP−2」
(ヤクルト生化学K.K.製)2gを添加し、3時間反
応させた。その後80℃に昇温して再び酵素を不活性化
させた。この反応液を常法により遠心分離機でエキス
層、油層、骨片等未分解層に分離し、エキス層は濾過後
60℃において減圧濃縮して水分40%以下のサバエキ
スを調製した。ミネラル成分、遊離アミノ酸群及びペプ
タイドアミノ酸群成分の割合を表4に示す。[Example 7] Mackerel 2.5 kg was put into a reaction can equipped with a stirrer together with 12 kg of water without pretreatment,
Heated to ° C. After 15 minutes, lower the temperature to 50 ° C and adjust the pH.
Commercially available Bacillus subtilis proteolytic enzyme in 8.0, trade name "Aroase AP-10" (Yakult Biochemistry KK
2 g), and reacted for 5 hours. Then, the temperature was raised to 80 ° C. and maintained for 15 minutes, then cooled to 50 ° C. and acetic acid was added to adjust the pH to 4.0. Proteolytic enzyme produced by Aspergillus oryzae, product name "Punchase NP-2"
(Yakult Biochemical KK) 2 g was added and reacted for 3 hours. Then, the temperature was raised to 80 ° C. to inactivate the enzyme again. This reaction solution was separated into an extract layer, an oil layer, an undegraded layer such as bone fragments by a centrifugal separator by a conventional method, and the extract layer was filtered and concentrated under reduced pressure at 60 ° C. to prepare a mackerel extract having a water content of 40% or less. Table 4 shows the proportions of the mineral component, the free amino acid group and the peptide amino acid group component.
【0034】[0034]
【比較例4】麹菌産生蛋白分解酵素を添加する際のpH
を6.0にした以外は、実施例6と同様に行い水分40
%以下のサバエキスを調製した。ミネラル成分、遊離ア
ミノ酸群及びペプタイドアミノ酸群成分の割合を表4に
示す。[Comparative Example 4] pH at the time of adding Aspergillus oryzae proteolytic enzyme
Was performed in the same manner as in Example 6 except that the water content was changed to 6.0.
% Mackerel extract was prepared. Table 4 shows the proportions of the mineral component, the free amino acid group and the peptide amino acid group component.
【0035】[0035]
【表4】 [Table 4]
Claims (2)
分解し、エキス分と廃物とに精製分離して魚貝藻類エキ
スを抽出する方法において、耐酸性蛋白分解酵素を作用
させて蛋白分解する際のpHを5.0未満とし、前記エ
キス分中にミネラル成分を溶出させることを特徴とする
多量ミネラル成分含有魚貝藻類エキスの抽出方法。1. A method of proteolytically degrading a raw fish and shellfish algae with a proteolytic enzyme to purify and separate into an extract and a waste product to extract a fish and shellfish algae extract, by acting an acid-resistant proteolytic enzyme. A method for extracting a fish and shellfish alga extract containing a large amount of mineral components, characterized in that the pH at the time is less than 5.0 and the mineral components are eluted into the extract.
せた後、pHを上げることを特徴とする請求項1記載の
多量ミネラル成分含有魚貝藻類エキスの抽出方法。2. The method for extracting a seaweed alga extract containing a large amount of mineral components according to claim 1, wherein the pH is raised after the mineral components are eluted in the extract.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP6155598A JP2696485B2 (en) | 1994-07-07 | 1994-07-07 | Extraction method of fish and shellfish algae extract containing large amount of mineral components |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP6155598A JP2696485B2 (en) | 1994-07-07 | 1994-07-07 | Extraction method of fish and shellfish algae extract containing large amount of mineral components |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH0819385A true JPH0819385A (en) | 1996-01-23 |
JP2696485B2 JP2696485B2 (en) | 1998-01-14 |
Family
ID=15609530
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP6155598A Expired - Lifetime JP2696485B2 (en) | 1994-07-07 | 1994-07-07 | Extraction method of fish and shellfish algae extract containing large amount of mineral components |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP2696485B2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002535364A (en) * | 1999-01-29 | 2002-10-22 | マーズ ユー ケー リミテッド | Antioxidant composition for pet animals and use thereof |
JP2011182723A (en) * | 2010-03-10 | 2011-09-22 | Tablemark Co Ltd | New seasoning composition |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59154966A (en) * | 1983-02-24 | 1984-09-04 | Sadao Nishimura | Preparation of sea tangle essence |
JPH06153863A (en) * | 1992-11-17 | 1994-06-03 | Mutsumi Shoji | Preparation of fish and shellfish extract prepared by removing heavy metal |
-
1994
- 1994-07-07 JP JP6155598A patent/JP2696485B2/en not_active Expired - Lifetime
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59154966A (en) * | 1983-02-24 | 1984-09-04 | Sadao Nishimura | Preparation of sea tangle essence |
JPH06153863A (en) * | 1992-11-17 | 1994-06-03 | Mutsumi Shoji | Preparation of fish and shellfish extract prepared by removing heavy metal |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002535364A (en) * | 1999-01-29 | 2002-10-22 | マーズ ユー ケー リミテッド | Antioxidant composition for pet animals and use thereof |
JP4966450B2 (en) * | 1999-01-29 | 2012-07-04 | マーズ ユー ケー リミテッド | Antioxidant composition for pet animals and method of use thereof |
JP2011182723A (en) * | 2010-03-10 | 2011-09-22 | Tablemark Co Ltd | New seasoning composition |
Also Published As
Publication number | Publication date |
---|---|
JP2696485B2 (en) | 1998-01-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Guérard et al. | Recent developments of marine ingredients for food and nutraceutical applications: a review. | |
Drummond et al. | Proteins recovery from meat processing coproducts | |
WO2005027657A1 (en) | Process for producing protein hydrolysate and protein hydrolysate | |
JP3117779B2 (en) | Novel peptide α-1000 | |
JPH06166697A (en) | Novel peptide, its preparation and use | |
JP2696485B2 (en) | Extraction method of fish and shellfish algae extract containing large amount of mineral components | |
JP2870871B2 (en) | A method for treating crustacean shells using enzymes | |
JP3787585B2 (en) | Angiotensin converting enzyme inhibitor, method for producing the same, and antihypertensive agent using the same | |
Akhila et al. | Seafood processing waste as a source of functional components: Extraction and applications for various food and non-food systems | |
JP2004073186A (en) | Method for producing amino acid component by enzymatic degradation of fish egg shell | |
JPS6287058A (en) | Novel peptide | |
JP5829844B2 (en) | Method for producing water-soluble elastin peptide | |
Das et al. | Fish protein hydrolysate production, treatment methods and current potential uses: A review | |
JP2960894B2 (en) | Production method of fermented seasonings | |
RU2207033C2 (en) | Method for wasteless complex reprocessing of chitin-containing raw material | |
JP2006042613A (en) | Method for producing harmful heavy metal-eliminated food material | |
JP3406341B2 (en) | New peptides, their production methods and applications | |
WO2012078223A1 (en) | A new method to produce marine proten hydrolysate with ultra-low heavy metal contamination | |
JP3518778B2 (en) | Method for preparing polyamine | |
JPH0420581B2 (en) | ||
JPH06239897A (en) | New hydrophilic peptide | |
JP3401280B2 (en) | New peptides, their production methods and applications | |
JP4512948B2 (en) | Useful amino acid composition, food or food compounding agent containing the same, and method for producing the same | |
CN114045323B (en) | Method for preparing fish skin and scale collagen peptide powder by multistage enzymolysis process | |
JP3281475B2 (en) | Zinc food material using seafood and its production method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313114 |
|
R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20070919 Year of fee payment: 10 |
|
FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20080919 Year of fee payment: 11 |
|
FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090919 Year of fee payment: 12 |
|
FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20100919 Year of fee payment: 13 |
|
FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110919 Year of fee payment: 14 |
|
FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110919 Year of fee payment: 14 |
|
FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120919 Year of fee payment: 15 |
|
FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130919 Year of fee payment: 16 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
EXPY | Cancellation because of completion of term |