JPH0819350A - Production of cultured pearl - Google Patents

Abstract

PURPOSE:To provide a pearl-culturing method in which pearl seeds are inoculated in the mother shells without loss of a cell-activating agent and a recovery- accelerator to develop these functions sufficiently thereby accelerating the growth of the pearl layers around the seed with death rate of the mother shells decreased. CONSTITUTION:An artificial pearl seed having a multilayer structure 5 and cell fragments of mantles 4 are inoculated into mother shells 1, respectively to form a coat layer 41 of mantle cells 4 around the seed 5 and the shells are cultured for pearl production wherein the pearl seeds 5 are impregnated with a recovery-accelerating agent for mother shells or a cell-activating agent or a combination thereof, then inoculated in the mother shells.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention relates to a method for producing cultured pearls, more specifically, a pearl nucleus consisting of a shell having a layered tissue and a cell piece consisting of a mantle are inserted into a mother oyster, and the cells are surrounded by the pearl nucleus. TECHNICAL FIELD The present invention relates to a method for producing cultured pearls, in which a cell bag composed of a piece-forming film is formed and cultured.

[0002]

2. Description of the Related Art Generally, during production of cultured pearls, mother oysters such as pearl oysters are subjected to an intubation procedure. In this intubation procedure, the shells of the oysters are opened with an opening device. It is fixed to a table, a scalpel is inserted from the opening of the shell to incise part of the fleshy part of the soft body in the mother shellfish, and from this incision into the soft body, first, the mantle taken from another shellfish is placed. Insert the cell pieces that are cut into small pieces,
Next, a spherical pearl nucleus made of a shell is inserted at the same position as the cell piece, and the pearl nucleus is brought into close contact with the cell piece. Then, the cell fragment undergoes cell division in the soft body portion and is regenerated to generate a cell bag that wraps around the pearl nucleus, and a pearl secretion mainly composed of calcium carbonate is produced from the cell bag. It is secreted and deposited around the pearl nucleus, and the nacre layer deposited on the pearl nucleus gradually grows to form a cultured pearl.

In addition, as a pretreatment before inserting the cell piece into a mother oyster, a cell activator is added to the cell piece with a spoid or the like in order to actively perform cell division in the soft body part of the cell piece. The cells are attached by dropping, and by inserting the cell debris to which the cell activator is adhered into the soft body part, the cell division of the cell debris in the soft body part is actively performed. The cell bag is generated surely and early so as to promote the growth of the nacre around the pearl nucleus by the cell bag.

[0004]

However, as described above, when the cell activator is attached to the cell debris, the cell debris of the soft body part cut with a scalpel from the shell opening of the mother shell is used. When inserted into the incision, the cell debris is brought into contact with these soft bodies and shells, and a part of the cell activator attached to the cell debris is easily lost. In particular, at the time of intubation treatment, the opening size of the shell is made as small as possible so as not to impair the activity of the mother shellfish,
Since the cell debris is inserted through the small opening of the shell, the cell debris easily contacts a part of the shell or the like, and the cell debris is moved to the inside of the incision of the soft body. Since the cell debris is brought into contact with a part of the incision portion or the soft body portion even when it is inserted, a part of the cell active agent is easily lost from the cell debris. When a part of the cell activator is lost from the cell debris, the activation effect of the cell debris by the cell activator is impaired, and cell division in the soft body part of the cell debris is actively performed. However, there is a problem that the production of the cell bag becomes uncertain or delayed, and the growth of the nacre around the pearl nucleus by the cell bag is deteriorated.

The above-mentioned cell activator is commercially available as a cell activator. By adhering to the cell debris, a cell activator for activating the vital activity of cells can be obtained, and the winding of beads is improved. However, there is a problem that the muscular mortality cannot be reduced even if the cytoactive agent is attached to the cells because it is insufficient for promoting and recovering action of the mussel.

[0006] The main purpose of the present invention is to ensure that the cell-activating agent can be inserted into the mother oysters without losing a part of the cell-activating agent at the time of performing the nuclear insertion procedure. Fully activates the activation effect of the cell debris by the cells, that is, activates cell division of the cell debris to generate the cell bag reliably and early, and the cell bag causes the growth of the nacre around the pearl nucleus. It is to provide a method of producing cultured pearls that can promote

Another object of the present invention is to provide a method for producing cultured pearls which can favorably restore the health of mother oysters after post-intubation and reduce the mortality rate thereof while promoting the growth of nacre. is there.

[0008]

In order to achieve the above object, the invention according to claim 1 is to insert a pearl nucleus 5 composed of a shell having a layered tissue and a cell piece 4 composed of a mantle into a mother shell 1. In a method for producing a cultured pearl, wherein a cell bag made of a film for producing the cell fragments 4 is formed in the pearl nucleus 5 to culture the pearl nucleus 5, the pearl nucleus 5 is impregnated with a cell activator, and then the mother shell 1 I decided to intubate.

According to the second aspect of the present invention, the pearl nucleus 5 is impregnated with a recovery accelerator for the mother pearl oyster 1 to give the pearl nucleus 5.
Was inserted into the mother oyster 1, and the invention according to claim 3 includes a cell activating agent in the recovery accelerating agent.

[0010]

[Function] At the time of performing the intubation operation, a cell formed by cutting a spherical pearl nucleus 5 composed of a shell having a layered tissue and a mantle taken from another shell into small pieces inside the mother shell 1 The piece 4 is inserted, and the mother clam 1 of the cell piece 4 is inserted.
The cell activator for actively performing cell division in the
It is not attached to the cell piece 4 side as in the conventional case, but is permeately supported on the pearl nucleus 5 side. That is, the invention according to claim 1 focuses on the fact that the shells such as freshwater bivalves such as the American blubbers and pig-toe shells that form the pearl nucleus 5 have a layered structure, By immersing the pearl nucleus 5 in the cell active agent, the cell active agent was permeated into the layered tissue of the pearl nucleus 5. Therefore, the cell activator can be reliably and firmly supported on the pearl nucleus 5, and as a result,
During the operation of inserting the pearl nucleus 5 into the mother shell 1, the pearl nucleus 5
Without losing some of the cell activator carried by,
The total amount of the cell activator carried on the pearl nucleus 5 can be reliably inserted into the mother shellfish 1 together with the pearl nucleus 5, and the pearl nucleus 5 can be closely attached to the mother shellfish 1 inside. The cell activator of the inserted cell piece 4 is sufficiently activated by the cell activator, that is, cell division of the cell piece 4 by the cell activator is actively carried out to reliably and early generate a cell bag. Thus, the growth of the nacre around the outer periphery of the pearl nucleus 5 by this cell bag can be promoted.

According to the second aspect of the invention, the pearl nucleus 5 is impregnated with the recovery promoter for the mother oyster 1, that is,
In order to promote the health recovery and growth of the mother oysters 1, the mother shellfish activator, which is used by being dissolved in seawater, is infiltrated into the pearl nucleus 5. The mortality of hay due to abnormal temperature or red tide can be reduced, the growth of nacre can be promoted, and the nacre with good winding can be formed. Moreover, since the cell activating agent according to claim 1 is included in the recovery accelerator according to claim 3, the growth of the nacre can be further promoted, and a higher quality nacre can be obtained.

[0012]

EXAMPLE FIG. 1 shows an example of a method for producing cultured pearls according to the present invention. In the figure, 1 is a mother shell made of pearl oysters and the like, and this mother shell 1 comprises a pair of left and right shells 2, 2. 2 and
Each of the shells 2 is provided with a soft body portion 3 located inside. The soft body part 3 includes a mantle 31 for secreting shell cortex and pearls to form the shell 2, a gonad 32 for performing a corectomy, a gill 33 having a respiratory function, and a foot having an ankle thread. 34
Etc. On the inner surface side of each of the shells 2, a pearl layer 21 composed of pearls secreted from the mantle 31 is formed.

However, at the time of performing an intubation operation, each of the shells 2 of the mother shell 1 is fixed to a pedestal in a state of being slightly opened by an opening device, and a female is inserted from the opening side of the shell 2 into the soft body. A cell piece 4 obtained by incising a part of the flesh of the gonad 32 in the part 3 and first cutting the mantle taken from another pearl oyster into a small piece into the inside of the gonad 32 from this incision. Then, a spherical pearl nucleus 5 consisting of a freshwater bivalve such as a US snail having a layered tissue at the same position as the cell piece 4 is closely inserted into the cell. Then, in the pearl nucleus 5, the gonad 32 of the cell piece 4 is formed.
The cell activator 6 for actively activating the cell division inside is permeated. In addition, the cell activator 6 is located in the pearl nucleus 5 by, for example, immersing the pearl nucleus 5 in the liquid cell activator 6 for 48 hours. The activator 6 can be permeated into the surface layer of the pearl nucleus 5 in a predetermined amount.

More specifically, the cell activator 6 is originally used by adhering to the cell debris 4 to activate the cell debris 4, and is commercially available, for example, EOSIN.Y.
(Manufactured by Wako Pure Chemical Industries, Ltd.) and the like are used. It is also possible to mix the cell activator 6 with, for example, a red colorant for coloring. When the cell activator 6 mixed with such a coloring agent is used, the outer surface thereof is colored red, so that the position of the pearl nucleus 5 when inserted into the gonad 32 can be easily confirmed. Therefore, when the pearl nucleus 5 is inserted into the same location as the cell piece 4, the pearl nucleus 5 can be inserted into the cell piece 4 in a precise and precise manner.

Further, as the pearl nucleus 5, for example, freshwater bivalve molluscs such as a blubber shell, a big toe shell shellfish and a niger head shellfish are used, and the shell shells of these shellfish are molded into a spherical shape and used for the intubation treatment. Such a shell, as shown in FIG.
Since each layered tissue 51 has a large number of laminated layered tissues 51 and each layered tissue 51 has innumerable pores, the cell active agent 6 can be penetrated into the pores of each layered tissue 51 to The cell activator 6 can be supported reliably and firmly.

Therefore, as described above, the layered tissue 51 of the pearl nucleus 5 is utilized to surely and firmly carry the cell activator 6 on the pearl nucleus 5 side, whereby the pearl nucleus 5 has the above-mentioned structure. The total amount of the cell active agent 6 contained in the pearl nucleus 5 is not lost in the gonal nucleus 5 during the operation of inserting the nucleus in the gon nucleus 32. It is possible to surely insert it together with 5. That is, at the time of performing the operation of inserting the pearl nucleus 5 into the gonad 32, the opening size of the shell 2 is made as small as possible so as not to impair the activity of the mother shell 1. Since the pearl nucleus 5 is inserted from a small opening, the pearl nucleus 5 may come into contact with a part of the shell 2, and the pearl nucleus 5 is incised in the gonad 32. Even when it is inserted into the inner part of the pearl nucleus 5, even though it is in contact with the incision and a part of the gonad 32, the layered tissue 51 is used in the pearl nucleus 5 to utilize the cell activating agent 6 Is reliably and firmly carried, the total amount of the cytoactive agent 6 carried on the pearl nucleus 5 is not lost at the time of the transection operation, and the total amount of the cytoactive agent 6 is carried on the gonad. It can be securely inserted in 32 together with the pearl nucleus 5, and Of the cell activator 6 carried on pearl nucleus 5, wherein in contact inserted and cell debris 4 into the gonads 32 in close contact shape and the pearl nucleus 5, the cell debris 4
It is possible to sufficiently exert the activating effect of the cell activator 6 on the.

Then, after the above-mentioned transcutting operation, the cell piece 4 undergoes cell division in the gonad 32 of the soft body part 3 to be regenerated, which is shown by a phantom line in FIG. A cell bag 4 that wraps around the outer periphery of the pearl nucleus 5
1 is produced, the pearl secretion mainly composed of calcium carbonate is secreted from the cell bag 41 and deposited around the pearl nucleus 5, and the pearl layer deposited on the pearl nucleus 5 gradually grows. The cultured pearls are formed. At this time, the cell activator 6 that promotes cell division of the cell pieces 4 is surely inserted into the gonad 32 together with the pearl nucleus 5 without being partially lost, The activation action of the cell piece 4 by the cell activator 6 can be sufficiently exerted, that is, the cell division of the cell piece 4 is actively performed to surely and early generate the cell bag 41, It is possible to promote the growth of the nacre around the pearl nucleus by the cell bag 41.

In the above embodiment, the pearl nucleus 5 is used.
Although the cell activator 6 is permeated in, the recovery promoter for the mother oyster 1 may be permeated therein.

This recovery promoter generally uses an activator for mother shells which is dissolved in seawater to promote the recovery of health of mother shellfish 1 and the growth thereof, and the recovery accelerator is permeated. By doing so, the health recovery of the mother oyster 1 can be promoted, and as a result, the resistance value of the mother oyster 1 against pest damage can be increased and the mortality of hay due to abnormally high temperature, abnormally low temperature, red tide, etc. can be reduced. .

Further, the above-mentioned recovery accelerating agent can promote the formation of a nacre and can form a high-quality nacre with good winding of the beads. good. By doing so, the formation of the nacre can be further promoted, and the nacre of higher quality can be formed.

[0021]

As described above, according to the invention described in claim 1, the pearl nucleus 5 made of a shell having a layered tissue and the cell piece 4 made of the mantle are inserted into the mother oyster 1 and the pearls are inserted. In a method for producing a cultured pearl in which a cell bag made of a film for producing the cell fragments 4 is formed in the nucleus 5 to culture the pearl, a cell-activating agent is allowed to penetrate into the pearl nucleus 5, and then the mother shell 1 is inoculated. Therefore, as in the conventional case, the cell debris 4
Since the cell activator is not attached to the above but is permeately carried on the pearl nucleus 5 side, the cell activator is reliably and firmly carried on the pearl nucleus 5 side. That is, since the pearl nucleus 5 is formed of a shell and the shell has a layered structure having a large number of pores, the pearl nucleus 5 is immersed in the cell activator, The layered tissue of the pearl nucleus 5 can be used to penetrate the cell active agent into the pearl nucleus 5 to securely and firmly support the cell active agent on the pearl nucleus 5 side. The total amount of the cell activator loaded on the pearl nucleus 5 without partially losing the cell activator loaded on the pearl nucleus 5 during the operation of inserting the pearl nucleus 5 into the mother shell 1. Securely in the mother shell 1 together with the pearl nucleus 5 It can be inserted into the mother shell 1 in close contact with the pearl nucleus 5 to fully exert the activation action of the cell debris 4 by the cell activator, that is, by the cell activator. The cell division of the cell pieces 4 is actively performed to surely and early produce the cell bag, and the growth of the nacre around the outer periphery of the pearl nucleus 5 by the cell bag can be promoted.

According to the second aspect of the invention, the pearl nucleus 5 is impregnated with the recovery accelerator for the mother oyster 1, that is,
In order to promote the health recovery and growth of the mother oysters 1, the mother shellfish activator, which is used by being dissolved in seawater, is infiltrated into the pearl nucleus 5. The mortality of hay due to abnormal temperature or red tide can be reduced, the growth of nacre can be promoted, and the nacre with good winding can be formed. Moreover, since the cell activating agent according to claim 1 is included in the recovery accelerator according to claim 3, the growth of the nacre can be further promoted, and a higher quality nacre can be obtained.

[Brief description of drawings]

FIG. 1 is a drawing showing an example of a method for producing cultured pearls according to the present invention.

FIG. 2 is an enlarged sectional view of a part of a pearl nucleus.

[Explanation of symbols]

 1 Mother Shell 4 Cell Fragments 41 Cell Bag 5 Pearl Nucleus 6 Cell Activator

Claims (3)

[Claims] 1. An artificial pearl nucleus (5) having a layered tissue and a cell fragment (4) consisting of a mantle are inserted into a mother oyster (1), and the pearl nucleus (5) contains the cell fragment (4) of the cell fragment (4). A method for producing a cultured pearl, which comprises culturing by forming a cell bag composed of a generative membrane, wherein the pearl nucleus (5) is impregnated with a cell-activating agent, and then the pearl nucleus (1) is inoculated. A method for producing cultured pearls characterized by: 2. An artificial pearl nucleus (5) having a layered tissue and a cell fragment (4) consisting of a mantle are inserted into a mother shell (1), and the pearl nucleus (5) contains the cell fragment (4). In the method for producing a cultured pearl, which comprises culturing by forming a cell bag composed of a productive membrane, the pearl nucleus (5) is impregnated with a mother shell recovery promoter,
A method for producing a cultured pearl, wherein the pearl nucleus (5) is inserted into the mother oyster (1). 3. The method for producing a cultured pearl according to claim 2, wherein the recovery accelerator contains a cell activator.