JPH08151323A - Method for treating and preventing swine respiratory organ disease and therapeutic agent therefor - Google Patents

Abstract

PURPOSE: To obtain a therapeutic agent capable of killing the causal bacteria of swine respiratory organ diseases, reducing the death selection rate, relieving the lesions of swine lungs and nasal cavities, and consequently achieving economical effects such as the stimulation of the growth, the improvement of feed demand rate and the shortage of shipment age in day. CONSTITUTION: A disinfectant, glutaral, is diluted with water containing a buffering agent. The obtained dilution solution is fogged in a particle diameter of <=10μm with a fogging machine or a high pressure atomizer and sucked by a swine. Thereby, the fog particles intrude into the respiratory bronchial tubes of the swine lower respiratory tract and further into the alveoli to kill respiratory organ disease-causing bacteria: Mycoplasma hyopneumoniae, Pasteurella multocida, Actinobacillus pluropneumoniae, Himophilas parasis, Bordetella bronchiseptica, etc., which always live in the swine.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for treating and preventing respiratory diseases in pigs and a therapeutic agent.

[0002]

2. Description of the Related Art In pig diseases, respiratory diseases and diarrhea are so well known that they immediately come to mind, and these diseases occur frequently in the field, of which diarrhea has been decreasing in recent years. Parasitic diseases (such as toxoplasmosis, roundworm, lungworm, paratidium, etc.) are still present, but their loss is very small compared to respiratory diseases.

The respiratory disease is viral (influenza) and the bacterial disease is M. hn (Mycoplasma hyopneumoniae), P. m (pasturera multocida),
A. PP (Actinobacillus pleuroneumonier),
H. PS (Hemophilus parasuisse), B.I. b (Boldetella bronchicepteca), AR (atrophic rhinitis), etc. are resident in the respiratory tract of most pigs, and even in SPF pigs (specific pathogen-free pigs), chronic opportunistic infections ( P.m.
A. PP, H.M. (PS etc.) is often resident.

Loss due to these respiratory diseases causes serious damage to breeding pigs and fattening pigs, and for the prevention and treatment of these diseases, suspended aqueous mycillin, which acts on Gram-negative positive bacteria, and black Rumphenicol, vicozamycin, sulfamonomethoxine, tiamulin, metacylinum, chlortetracycline, oxytetracycline, amoxyline, oxophosphoric acid, sulfamonomethoxine and the like are used as injection, oral administration and feed additives.

[0005] Further, for disinfection of pig houses and the like, inverse soaps, amphoteric soaps, ortho agents, iodine holes and the like are used, but at present, satisfactory effects have not been obtained. In other words, a disinfectant used for livestock barn is particularly desired to have a sterilizing power that does not decrease under any conditions, but disinfectants satisfying such conditions have not easily appeared. .

[0006]

As described above, injection, oral administration, and feed addition of various antibiotics have been performed for respiratory diseases of pigs, but they have not exhibited satisfactory effects.
Although piggeries are also disinfected, there is no idea or report that these disinfectants are expected to have the effect of killing bacteria that cause respiratory diseases that are resident in the respiratory tract.

The inventors of the present invention changed their ideas and thought that it would be possible to treat and prevent respiratory diseases in pigs using the disinfectant glutaral used in the piggery. Thus, the present inventors have found that bacteria that cause respiratory illness in pigs (resident bacteria in the respiratory tract) can be killed and completed the present invention.

[0008]

According to a first aspect of the present invention, the disinfectant glutaral is diluted with water containing a buffer, and the resulting diluted liquid is used with a smoke atomizer or a high-pressure micromachine to make the particle diameter 10 μm or less. By inhaling fumes and inhaling them into the respiratory tracts of the lower respiratory tract of the pigs, as well as into the alveoli, inhaling fumes particles, the respiratory pathogens M. hn (Mycoplasma
High Pneumoniae), P. m (pasturera maltocida), A. PP (Actinobacillus pleuropneumoniae), H.P. PS (Hemophilus parasuisse), B.I. b
(Bordetera Bronquiseptica) is a method of treating respiratory diseases in pigs, which is characterized by dying.

The invention of claim 2 is characterized by using a diluent obtained by diluting a preparation containing 25% glutaral with water to which a buffer of substantially the same amount as that of the glutaral preparation is added by 10 to 100 times. This is a method for treating respiratory diseases.

A third aspect of the present invention is a method for treating respiratory disease in pigs, which comprises spraying 0.5L to 10L of the diluted liquid per 100 tsubo of pig pen.

A fourth aspect of the present invention is a method for preventing respiratory disease in pigs, which comprises periodically fogging the adjusted diluent to prevent respiratory disease in pigs.

[0012] The invention of claim 5 is a respirator which is present in a pig, which is caused by inhaling fumes with a particle size of 10 μm or less and inhaling them into the respiratory bronchi of the lower respiratory tract of the pig and further into the alveoli. Disease-causing bacterium M. hn (Mycoplasma hyopneumoniae), P. m (pasturera multocida),
A. PP (Actinobacillus pleuroneumonier),
H. PS (Hemophilus parasuisse), B.I. b (Bordetera Bronxepteca) and the like, which is a therapeutic agent for killing glutaral 25%, is diluted 10 to 100 times with water containing a buffer agent in an amount approximately equal to that of the glutaral preparation. It is a therapeutic agent for respiratory diseases in pigs characterized by

[0013]

In the present invention, the disinfectant glutaral is diluted with water to which a buffer is added, and the resulting diluted solution is atomized with a mist or a high-pressure micromachine to a particle size of 10 μm or less, and sucked by a pig. The respiratory tract of the lower respiratory tract of pigs is infiltrated with mist particles into the respiratory tract and further into the alveoli. hn (Mycoplasma hyopneumoniae),
P. m (pasturera maltocida), A. PP (Actinobacillus pleuropneumoniae), H.P. PS (Hemophilus parasuisse), B.I. b (Bordetera Bronquiseptica) etc. can be killed, so that the pig is killed,
Reduction of selection rate and reduction of lesions of lung and nasal cavity are observed, and as a result, economic effects such as promotion of growth, improvement of feed requirement rate and shortening of shipping date can be exhibited.

As glutaral preparations used in the present invention, glutaclean (trade name, manufactured by Yashima Sangyo Co., Ltd.) and glutar Z (trade name, manufactured by Yashima Sangyo Co., Ltd.) are known.

Glutaclean is a glutaraldehyde (general name: glutaral) preparation (a preparation containing 25% glutaral), which is the world's first bactericidal disinfectant for veterinary medicine. Glutaclean is also the best disinfectant for livestock, with no reduction in bactericidal activity even in the presence of organic substances. The component is a kind of aldehyde, but does not have a strong pungent odor like formalin (formaldehyde).

Glutar Z has a glutaral content substantially equal to that of glutaclean. However, since the buffer of glutar Z is a liquid, dissolution for dilution is easy and handling is simple.

The above-mentioned glutaclean and gluter Z have been approved as animal medicines for disinfecting livestock houses, poultry houses and equipment, eggs, and poultry raising equipment. Hereinafter, the component amounts of glutaclean and glutar Z, the dosage, and the germicidal spectrum of glutaral, which are commonly used, will be described.

1) Amount of ingredients Glutaclean (main ingredient) 100 mL (milliliter)
Contains 25 g of medium glutaraldehyde (glutaral). (Buffer) Contains 60 g of sodium bicarbonate and 40 g of sodium carbonate in 100 g. Glutar Z (main ingredient) contains 25 g of glutaraldehyde in 100 mL. (Buffer) Contains 300 g of potassium acetate and 34 g of disodium phosphate in 300 mL.

2) Dosage Glutaclean is a preparation that is adjusted when used. The glutaclean formulation is diluted with water as desired. Diluent 10L
Mix and use 1.5 g of glutaclean buffer per liter. However, when used for surgery or disinfection of dissection instruments and equipment, add 25.5 g of glutaclean buffer per 10 L of diluent.

[How to use GlutaClean]

[Table 1]

Gluter Z is a preparation for use at the time of use. The gluter Z base is diluted with water according to the purpose. Diluent 10
Mix and use 7.5 mL of buffer per L. However, when used for disinfection of surgical dissection equipment, add and mix 30 mL of gluter Z buffer per 1 L of diluent.

[How to Use Gluter Z]

[Table 2] Since gluter Z is a liquid, it is easily dissolved and easy to handle, and is suitable for fine mist disinfection.

3) Sterilization spectrum of glutaral

[Table 3] As mentioned above, glutaral is used for disinfection of livestock and poultry houses, but its disinfection spectrum is a wide range of disinfectants, including gram-negative bacteria, gram-positive bacteria, fungi and viruses.

As a result of various studies, the inventors of the present invention have conducted various investigations to directly inhale this disinfectant glutaral into pigs to kill the bacteria causing respiratory diseases and to treat / prevent respiratory diseases in pigs. , Glutaral diluting solution with a mist or high-pressure micromachine to reduce particle size to less than 10μ in a pig house to cause inhalation of respiratory particles in the lower respiratory tract of the pig and further into the alveoli, resulting in persistent respiratory disease. The causative bacterium M. hn
(Mycoplasma hyopneumoniae), P. m (pasturera maltocida), A. PP (Actinobacillus pleuropneumoniae), H.P. PS (Hemophilus parasuisse), B.I. It has been found that b (Boldetella bronchiseptica) and the like can be killed.

It was also found that the death of these causative bacteria can prevent and treat respiratory diseases.

[0026]

EXAMPLES Test examples and examples in the present invention will be described below. <Test Example> Test Example I Regarding Glutaral Diluted Solution From the dosages of Glutaclean and Gluter Z shown in 2) above, 1 of Glutaclean and Gluter Z was used.
It was considered appropriate to dilute it 0 to 100 times, and the glutaral buffer was considered to be equivalent to the amounts of glutaclean and gluta Z used. Particle Size The diluted solution prepared above was sprayed with a sprayer (particle size 100 μm or more) so as to be 5 L per 100 tsubo, and finely atomized with a fine mist (particle size 11 to 99 μm) (control). Also,
The above diluted solution was fogged (particle size: 10 μm or less) by a haze machine and 5 μm (particle size: 10 μm or less) by a high-pressure micromachine so that the diluted solution became 5 L per 100 tsubo (invention).

The inhibitory effects were compared by sampling and examining the respiratory pathogens in the lower respiratory tract due to the difference in particle size. The results are shown in Table 4. The numbers in the table are the number of detections /
Indicates the number of test animals.

[Table 4] (Note) P. m: Pasteurellosis A. PP: Pleural pneumonia H. PS: Glacer's disease According to the above-mentioned test that conducted a sampling test, if the particle size is 10 μm or less, it causes respiratory bronchitis in the lower respiratory tract, and glutaral particles invade the alveoli to kill the bacteria causing respiratory diseases. , I found that I could treat respiratory diseases. The pigs were also found to gain weight. It has also been found that regular use of the fumes can prevent respiratory diseases. In contrast, the control failed to effectively kill the causative organism of the respiratory disease. It is considered that this is because the particles cannot penetrate deep into the respiratory tract of the pig due to the large particle size in the case of spraying and fine mist, and therefore a sufficient effect cannot be obtained. Therefore, it is judged that the respiratory illness in the respiratory tract cannot be suppressed by spraying or fine mist.

Test Example II Haze test in a pig house of glutaral 1) Pig house 136 tsubo 56 fattening pigs The pig house was 12 mx 37.6 m = 451.2 m ² (136.
Each of the pigs has a total of 8 pigs in each of the four rooms on each side of the central aisle, surrounded by a 1-meter-high fence, and each pig holds 7 pigs. The upper part of each pig bun communicates. 2) Preparation of drug-Gluter Z 25-fold diluted solution 2.8 L of gluter Z main agent and 2.8 L of buffer solution are diluted with water to 70 L. -Gluter Z 50-fold diluted solution 1.4 L of gluter Z main agent and 1.4 L of buffer solution are diluted with water to 70 L. -Gluter Z 100-fold diluted solution 0.7 L of gluter Z base and 0.7 L of buffer solution are diluted with water to 70 L. 3) Amount of fumes medicine per pig house 13.6L (0.1L /
4) Spray method 4) Sprayers wore rubber gloves and a dust mask, held a spray fume machine, and smoked alternately from the central passage to the left and right pig bunches. The fume time was about 5 minutes per pig bun. 5) Individual exposure concentration measurement of workers In this test, the reagent reagent dilution ratio was 25 times, 50 times, 1
The individual exposure concentration of glutaral in workers in 00 times spraying work was 0.24ppm, 0.18ppm,
The exposure concentration was 0.1 ppm, and the lower the dilution ratio, the higher the exposure concentration. It was necessary to wear protective equipment for exposure protection, but no abnormalities were observed in the workers.

From the above, it was determined that it was appropriate to apply a 10 to 100-fold diluted solution to a pig barn of 100 tsubo in an amount of 0.5 to 10 L.

Test Example III Measurement Test of Time-Dependent Change in Concentration in Air of Diluted Solution When the diluting solutions of Test Example II2) were sprayed into a pig pen, fluctuations in the air concentration of glutaral were examined. The results are shown in FIG. Thus, the 25-fold dilution, the 50-fold dilution, and the 100-fold dilution all had the highest geometric mean concentration immediately after spraying, and 25 hours diluted 1 hour after spraying,
When diluted 50 times, the value was 30% or less immediately after spraying, and when diluted 100 times, the value was 60% or less. Three hours after spraying, the geometric mean concentration was 0.02 ppm (below the detection limit) at all dilution factors. It was still 0.02 ppm after 24 hours.

Test Example IV Toxicity Comparison between Glutaral and Formalin Toxicity comparison between glutaral and formalin used in the present invention was carried out. The results are shown in Tables 5 and 6.

[Table 5] Glutaral 100% conversion

[0032]

[Table 6] Glutaral is a liquid, does not inhale, has low toxicity, and can be used safely, but formalin is gaseous at room temperature and is dangerous for workers to inhale.

Test Example V Therapeutic effect test of pigs with onset lesions A pig house containing pigs with onset lesions was subjected to the test of spraying the diluted solution twice under the same conditions as in Test Example I. The results are as follows. Met. In Test 1, when fogging was performed on 20 affected lesion pigs, 14 of the 20 affected lesion pigs were identified as controls, but in the present invention, the number of pigs was reduced to 1 and the weight of the pig was also increased. The economic effect was improved. In Test 2, when fumes were applied to 28 affected lesion pigs,
As a control, 12 out of 18 affected pigs were found, but in the present invention, the number was reduced to one, the weight was increased, and the economic effect such as improvement of feed conversion rate and shortening of shipping date could be achieved. .

Further, in a pig house having an AR lesion in the turbinate of the pig, a 70-fold dilution of glutaral was applied to a pig house 100 times.
When 20 L of smoke was applied to the tsubo, the number of severely affected pigs decreased to 4 out of 9 pigs, and it was found that the pigs also exhibited a therapeutic effect on AR disease (atrophic rhinitis).

<Example> Example 1 Glutaral 200 m, the main ingredient of Glutaclean
200 g of buffer (120 g of sodium bicarbonate, 80 g of sodium carbonate) was added to L, and water was further added to make 2 L to make a 10-fold diluted solution.

Example 2 200 ml of a buffering agent was added to 200 ml of a main ingredient of Gluter Z, glutaral, and water was further added to make 20 liters to prepare a 100-fold diluted solution.

Example 3 In a pig house with a pneumonia lesion in a pig, 0.5 L of the diluted solution of Example 1 was sprayed with respect to 100 tsubo of the pig house. There was a 78.2% reduction in disease.

Example 4 When 2 L of the diluted solution of Example 2 was sprayed into a pig house having a pneumonia lesion in a pig with respect to 100 tsubo of the pig house, it was 77.2% as compared with the control group which was sprayed or atomized.
The disease was alleviated, the weight gained and the economic effect was exhibited.

Example 5 Pig A. When 2L of 10 times diluted glutaral diluted solution was sprayed to 100 tsubo of a pig house in a pig house with the risk of PP (Actinobacillus pleuropneumoniae), 3 pigs with severe symptoms were 0, 2 pigs with mild symptoms were also 0, A. Suppression of PP is completely recognized, and promotion of growth
Feed conversion rates have also improved.

Example 6 In a pig house where there is a risk of respiratory disease in pigs, 5 L of a 50-fold diluted glutaral solution was sprayed on 100 tsubo of a pig house, and compared with the control group which was sprayed or atomized, it was 77.6. % Of pigs gained weight.

Example 7 In a pig house with AR lesions in the turbinates of pigs, when a 100-fold dilution of glutaral was inhaled in a volume of 10 L per 100 tsubo of the pig house, the number of affected lesion pigs was reduced to 4 out of 9 pigs, and the therapeutic effect was reduced. Demonstrated.

[0042]

As described above in detail, according to the present invention, glutaral is diluted to 10 to 100 times the amount thereof,
By inhaling 0.5 to 10 L of smoke, bacteria causing respiratory diseases of pigs are killed, resulting in reduction of mortality and selection of lung and nasal lesions, resulting in promotion of growth and improvement of feed requirement rate. Economic effects such as improvement and shortening of shipping date can be achieved, and excellent effects of preventing respiratory disease in pigs can be achieved.

[Procedure amendment]

[Submission date] December 14, 1994

[Procedure Amendment 1]

[Document name to be amended] Statement

[Name of item to be corrected] 0030

[Correction method] Change

[Correction content]

[0030] When each dilution dilution of aging Measurement Test Test Example II2) of Air Concentration in Test Example III dilutions were examined for variations in air concentrations glutaral in case of fumes pig house, 2 5-fold dilution , 50 times and 100 times dilution all have the highest geometric mean concentration immediately after spraying,
One hour after the application, the dilution was 25 times, the 50 times dilution was 30% or less immediately after the application, and the 100 times dilution was 60% or less. Three hours after spraying, the geometric mean concentration was 0.02 ppm (below the detection limit) at all dilution factors.
It was still 0.02 ppm after 24 hours.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Yoichi Sato 2-5-12 Tabatagiriyama, Narita, Azumi-cho, Koriyama-shi, Fukushima Prefecture (72) Takenori Saiga 757-1, Futako, Takatsu-ku, Kawasaki-shi, Kanagawa Yashima Sangyo Incorporated (72) Inventor Yoichiro Tsubaki, 757-1, Futako, Takatsu-ku, Kawasaki, Kanagawa, Yashima Sangyo Co., Ltd. (72) Inventor, Junichi Takada, 757-1, Futako, Takatsu, Kawasaki, Kanagawa, Yashima Sangyo, Ltd.

Claims (5)

[Claims] 1. The disinfectant glutaral is diluted with water to which a buffer is added, and the resulting diluted solution is atomized to a particle size of 10 μm or less using a fog machine or a high-pressure micromachine and sucked by the pig. The respiratory tract bronchi in the lower respiratory tract, and further infiltrate fumes into the alveoli, causing the respiratory bacterium M. hn
(Mycoplasma hyopneumoniae), P. m (pasturera maltocida), A. PP (Actinobacillus pleuropneumoniae), H.P. PS (Hemophilus parasuisse), B.I. A method for treating respiratory disease in pigs, which comprises killing b (bordetera bronxeptheca) and the like. 2. A formulation containing 25% of glutaral is added with water containing a buffering agent in an amount approximately equal to that of the glutaral formulation, and the mixture is added to 10 to 10 parts of water.
The method for treating respiratory disease in pigs according to claim 1, wherein a dilute solution diluted to 0-fold is used. 3. The diluent prepared per 100 tsubo of pork house is diluted with 0.1 g
The method for treating swine respiratory disease according to claim 1 or 2, wherein fumes of about 5 L to 10 L are produced. 4. A method for preventing respiratory disease in pigs, which comprises periodically performing the method according to claim 1, 2 or 3 to prevent respiratory disease in pigs. 5. A respiratory disease-causing bacterium that is resident in pigs, M. cerevisiae, is caused by inhaling the smoke particles with a particle diameter of 10 μm or less and inhaling them into the respiratory bronchi of the lower respiratory tract of the pigs and further into the alveoli. hn (Mycoplasma hyopneumoniae),
P. m (pasturera maltocida), A. PP (Actinobacillus pleuropneumoniae), H.P. PS (Hemophilus parasuisse), B.I. b (bordetera bronxepteca) and the like, which is a therapeutic agent for killing, etc., a 25% glutaral preparation is diluted 10 to 100-fold with water to which an approximately equivalent amount of a buffer is added. A therapeutic agent for respiratory disease in pigs characterized by being