JP2637703B2 - Method for treating / preventing respiratory disease in swine and therapeutic agent - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for treating and preventing respiratory diseases in pigs and a therapeutic agent.

[0002]

2. Description of the Related Art In pig diseases, respiratory diseases and diarrhea are so well known that they immediately come to mind, and these diseases occur frequently in the field, of which diarrhea has been decreasing in recent years. Parasitic diseases (such as toxoplasmosis, roundworm, lungworm, paratidium, etc.) are still present, but their loss is very small compared to respiratory diseases.

[0003] Respiratory diseases are viral (influenza) and bacterial are Mycoplasma hyopneumoniae.
D (Mycoplasma hyopneumonia)
e), Pasteurella maltosida (Pasteurell)
a multicida), Actinobacillus pullo
Pneumoniae (Actinobacillus ple)
uropneumoniae), Haemophilus pallas
Chair (Haemophilus parasuis),
Bordetella Bronxepeptica
b.n. branchiceptica) , AR (atrophic rhinitis), etc. are resident in the respiratory tract of most pigs, and SP
Chronic opportunistic infections ( Pasturella maltosida , Actinobacillus pullo ) even in F pigs (pigs without specific pathogens)
Pneumoniae, haemophilus paraswiss ) are often resident.

[0004] The loss caused by these respiratory diseases has occurred in breeding pigs and fattening pigs, and has caused great damage. Preventive treatment for these diseases requires aqueous suspension of mycillin, which acts on Gram-negative bacteria, and Ramphenicol, bicozamycin, sulfamonomethoxine, tiamulin, metasilinam, chlortetracycline, oxytetracycline, amoxicillin, oxophosphoric acid, sulfamonomethoxine, and the like have been used as injections, oral administration, and feed additives.

[0005] Further, for disinfection of pig houses and the like, inverse soaps, amphoteric soaps, ortho agents, iodine holes and the like are used, but at present, satisfactory effects have not been obtained. In other words, a disinfectant used for livestock barn is particularly desired to have a sterilizing power that does not decrease under any conditions, but disinfectants satisfying such conditions have not easily appeared. .

[0006]

As described above, injection, oral administration, and feed addition of various antibiotics have been performed for respiratory diseases of pigs, but they have not exhibited satisfactory effects.
Although piggeries are also disinfected, there is no idea or report that these disinfectants are expected to have the effect of killing bacteria that cause respiratory diseases that are resident in the respiratory tract.

The inventors of the present invention changed their ideas and thought that it would be possible to treat and prevent respiratory diseases in pigs using the disinfectant glutaral used in the piggery. Thus, the present inventors have found that bacteria that cause respiratory illness in pigs (resident bacteria in the respiratory tract) can be killed and completed the present invention.

[0008]

According to a first aspect of the present invention, a disinfectant glutaral is diluted with water to which a buffer has been added, and the resulting diluent is reduced to a particle size of 10 μm or less using a fume machine or a high-pressure fine machine. and fumes, respiratory bronchial lower respiratory tract of pigs by suction pigs, by entering the fume particles to further alveolar, respiratory disease caused by bacteria mycoplasma Haionyu that resident pigs
-Monnier (Mycoplasma hyopneumo)
niae), Pasteurella maltosida (Pasteur)
ella multicida), Actinobacillus.
Plururo pneumoniae (Actinobacillus)
pleuropneumoniae ), haemophilus
Para Swiss (Haemophilus parasui)
s), Bordetella Bronxepeptica (Bordete)
a method of treating respiratory disease in pigs, which comprises killing C. lla bronchiseptica) .

The invention of claim 2 is characterized by using a diluent obtained by diluting a preparation containing 25% glutaral with water to which a buffer of substantially the same amount as that of the glutaral preparation is added by 10 to 100 times. This is a method for treating respiratory diseases.

[0010] The invention of claim 3 is a method for treating respiratory diseases of pigs, wherein 0.5 L to 10 L of the diluted solution adjusted per 100 tsubo of pig house is smoked.

A fourth aspect of the present invention is a method for preventing respiratory disease in pigs, which comprises periodically fogging the adjusted diluent to prevent respiratory disease in pigs.

According to a fifth aspect of the present invention, there is provided a respiratory system which is resident in pigs by fogging the particles with a particle diameter of 10 μm or less and inhaling them into the respiratory bronchi of the lower respiratory tract of the pigs and further into the alveoli. Disease-causing bacteria Mycoplasma hyopneumoniae
(Mycoplasma hyopneumonia
e), Pasteurella maltosida (Pasteurell)
a multicida), Actinobacillus pullo
Pneumoniae (Actinobacillus ple)
uropneumoniae), Haemophilus pallas
Chair (Haemophilus parasuis),
Bordetella Bronxepeptica
A therapeutic agent for killing bronchiseptica), 10 to 100 and glutaral 25% containing formulations with water plus a buffer of the glutaric formulation substantially equivalent
It is a remedy for swine respiratory disease characterized by being diluted two-fold.

[0013]

According to the present invention, the disinfectant glutaral is diluted with water to which a buffer has been added, and the obtained diluted liquid is smoked with a particle size of 10 μm or less using a fogger or a high-pressure finer, and sucked into pigs. respiratory bronchial lower respiratory tract of pigs by entering the fume particles to further alveolar, respiratory disease caused by bacteria while resident in pigs
Icoplasma Hio Nyumonie (Mycoplas)
ma hyopneumoniae), Pasteurella ma
Russida (Pasteurella multicid)
a), Actinobacillus pleuropneumoniae (Act
inobacillus pleuropneumonon
iae), Haemophilus para swiss (Haemph)
ilus parasuis), Bordetella bronchi
Septica (Bordetella bronchise)
ptica) can be killed, thereby reducing pig mortality, reducing culling rates, reducing lesions in the lungs and nasal passages, resulting in economic growth such as accelerated growth, improved feed demand and reduced shipping dates. The effect can be exhibited.

As glutaral preparations used in the present invention, glutaclean (trade name, manufactured by Yashima Sangyo Co., Ltd.) and glutar Z (trade name, manufactured by Yashima Sangyo Co., Ltd.) are known.

Glutaclean is a glutaraldehyde (general name: glutaral) preparation (a preparation containing 25% glutaral), which is the world's first bactericidal disinfectant for veterinary medicine. Glutaclean is also the best disinfectant for livestock, with no reduction in bactericidal activity even in the presence of organic substances. The component is a kind of aldehyde, but does not have a strong pungent odor like formalin (formaldehyde).

Glutar Z has a glutaral content substantially equal to that of glutaclean. However, since the buffer of glutar Z is a liquid, dissolution for dilution is easy and handling is simple.

The above-mentioned glutaclean and gluter Z have been approved as animal medicines for disinfecting livestock houses, poultry houses and equipment, eggs, and poultry raising equipment. Hereinafter, the component amounts of glutaclean and glutar Z, the dosage, and the germicidal spectrum of glutaral, which are commonly used, will be described.

1) Component amount Glutaclean (base) 100 mL (milliliter)
Contains 25 g of medium glutaraldehyde (glutaral). (Buffer) Contains 60 g of sodium bicarbonate and 40 g of sodium carbonate in 100 g. Glutar Z (main ingredient) contains 25 g of glutaraldehyde in 100 mL. (Buffer) Contains 300 g of potassium acetate and 34 g of disodium phosphate in 300 mL.

2) Dosage Glutaclean is a preparation that is adjusted when used. The glutaclean formulation is diluted with water as desired. Diluent 10L
Mix and use 1.5 g of glutaclean buffer per liter. However, when used for surgery or disinfection of dissection instruments and equipment, add 25.5 g of glutaclean buffer per 10 L of diluent.

[How to use GlutaClean]

[Table 1]

Gluter Z is a preparation for use at the time of use. The gluter Z base is diluted with water according to the purpose. Diluent 10
Mix and use 7.5 mL of buffer per L. However, when used for disinfection of surgical dissection equipment, add and mix 30 mL of gluter Z buffer per 1 L of diluent.

[How to use gluter Z]

[Table 2] Since gluter Z is a liquid, it is easily dissolved and easy to handle, and is suitable for fine mist disinfection.

3) Sterilization spectrum of glutaral

[Table 3] As mentioned above, glutaral is used for disinfection of livestock and poultry houses, but its disinfection spectrum is a wide range of disinfectants, including gram-negative bacteria, gram-positive bacteria, fungi and viruses.

The present inventors have conducted various studies to ascertain whether or not inhaling the disinfectant glutaral directly into pigs can kill bacteria causing respiratory diseases and treat or prevent respiratory diseases in pigs. Glutaral dilute solution is reduced to 10μ or less with a fogger or a high-pressure micromachine to reduce the size of particles to 10μ or less in a piggery, thereby invading the respiratory bronchi in the lower respiratory tract of pigs, and further into the alveoli, thereby causing resident respiratory disease. Causative fungus mycop
Razuma Hio Nyumonie (Mycoplasma)
hyopneumoniae), Pasteurella maltosi
Da (Pasteurella multitocida),
Actinobacillus pleuropneumoniae (Actino
bacillus pleuropneumonia
e), Haemophilus para-Swiss
us parasuis), Bordetella Bronxep
Chica (Bordetella bronchisept)
ica) can be killed.

Further, it has been found that the prevention and treatment of respiratory diseases can be achieved by killing these causative bacteria.

[0026]

EXAMPLES Hereinafter, test examples and examples in the present invention will be described. <Test Example> Test Example I With respect to the diluent of glutaral, it is considered appropriate to dilute glutaclean and glutar Z 10 to 100 times from the dosage of glutaclean and glutar Z shown in the above 2) dosage, The diluent was adjusted, considering that it is appropriate to use the same amount of glutaral buffer as glutaclean and gluter Z. The diluent adjusted for the particle diameter as described above was sprayed with a sprayer (particle diameter of 100 μ or more) and finely misted with a fine atomizer (particle diameter of about 11 to 99 μ) per 100 tsubo (control). Further, the above-mentioned diluted solution was subjected to fume (particle size of 10 μm or less) with a fume machine and fume (particle size of 10
μ or less) (the present invention).

The inhibitory effects were compared by sampling and examining the respiratory pathogens in the lower respiratory tract due to the difference in particle size. The results are shown in Table 4. The number in the table is the number of detections /
Indicates the number of test animals.

[Table 4] According to the above test in which the sampling test was conducted, if fumes with a particle diameter of 10 μ or less were found, respiratory bronchitis in the lower respiratory tract and glutaral particles penetrated into the alveoli, killing bacteria causing respiratory disease, Can be treated. The pigs were also found to gain weight. It has also been found that regular use of the fumes can prevent respiratory diseases. In contrast, the control failed to effectively kill the causative organism of the respiratory disease. This is considered to be because the particles cannot be penetrated deep into the respiratory tract of pigs due to the large particle size in spraying and fine mist, and therefore, a sufficient effect cannot be obtained. Therefore, it is determined that respiratory illness in the airway cannot be suppressed by spraying or fine mist.

Test Example II Haze test in a pig house of glutaral 1) Pig house 136 tsubo 56 fattening pigs The pig house was 12 mx 37.6 m = 451.2 m ² (136.
Each of the pigs has a total of 8 pigs in each of the four rooms on each side of the central aisle, surrounded by a 1-meter-high fence, and each pig holds 7 pigs. The upper part of each pig bun communicates. 2) Preparation of drug-Gluter Z 25-fold diluted solution 2.8 L of gluter Z main agent and 2.8 L of buffer solution are diluted with water to 70 L. -Gluter Z 50-fold diluted solution 1.4 L of gluter Z main agent and 1.4 L of buffer solution are diluted with water to 70 L. -Gluter Z 100-fold diluted solution 0.7 L of gluter Z base and 0.7 L of buffer solution are diluted with water to 70 L. 3) Amount of smoke medicine per pig house 13.6 L (0.1 L /
4) Spray method 4) Sprayers wore rubber gloves and a dust mask, held a spray fume machine, and smoked alternately from the central passage to the left and right pig bunches. The fume time was about 5 minutes per pig bun. 5) Measurement of personal exposure concentration of workers In this test, the dilution ratio of the reagent was 25 times, 50 times,
The glutaral personal exposure concentration of the worker in the spraying operation of 00 times was 0.24 ppm, 0.18 ppm, respectively.
0.1 ppm, and the lower the dilution ratio, the higher the exposure concentration. It was necessary to wear protective equipment for exposure protection, but no abnormalities were observed in the workers.

From the above, it was determined that it was appropriate to apply a 10 to 100-fold diluted solution to a pig barn of 100 tsubo in an amount of 0.5 to 10 L.

Test Example III Time-Dependent Change Measurement of Air Concentration in Diluted Solution When the dilutions of each dilution in Test Example II2) were smoked in a pig barn, the change in air concentration of glutaral was examined. Both 50-fold dilution and 100-fold dilution have the highest geometric average concentration immediately after spraying,
One hour after spraying, the value was 30% or less immediately after spraying at 25-fold dilution and 50-fold dilution, and 60% or less at 100-fold dilution. Three hours after spraying, the geometric mean concentration was 0.02 ppm (below the detection limit) at all dilution factors.
It was still 0.02 ppm after 24 hours.

Test Example IV Comparison of Toxicity between Glutaral and Formalin The toxicity of glutaral and formalin used in the present invention was compared. The results are shown in Tables 5 and 6.

[Table 5] Glutaral 100% conversion

[0032]

[Table 6] Glutaral is a liquid, does not inhale, has low toxicity, and can be used safely, but formalin is gaseous at room temperature and is dangerous for workers to inhale.

Test Example V Therapeutic Effect Test of Pigs with Onset Lesion In a pig house in which pigs with onset lesion were previously placed, a test in which the diluent was smoked under the same conditions as in Test Example I was performed twice. Met. In Test 1, when fogging was performed on 20 affected lesion pigs, 14 of the 20 affected lesion pigs were identified as controls, but in the present invention, the number of pigs was reduced to 1 and the weight of the pig was also increased. The economic effect was improved. In Test 2, when fumes were applied to 28 affected lesion pigs,
In the control, 12 out of 18 affected pigs were found, but in the present invention, the number of pigs was reduced to 1 and the weight was increased, so that economic effects such as improvement of feed request rate and shortening of shipping date could be obtained. .

Further, in a pig house having an AR lesion in the turbinate of the pig, a 70-fold dilution of glutaral was applied to a pig house 100 times.
When 20 L of smoke was applied to the tsubo, the number of severely affected pigs decreased to 4 out of 9 pigs, and it was found that the pigs also exhibited a therapeutic effect on AR disease (atrophic rhinitis).

Example 1 Example 1 Glutaral's main ingredient, glutaral 200 m
200 g of buffer (120 g of sodium bicarbonate, 80 g of sodium carbonate) was added to L, and water was further added to make 2 L to make a 10-fold diluted solution.

Example 2 200 mL of a buffer was added to 200 mL of glutaral, the main ingredient of gluter Z, and water was further added to make 20 L to make a 100-fold diluted solution.

Example 3 In a pig house with a pneumonia lesion in a pig, 0.5 L of the diluted solution of Example 1 was sprayed with respect to 100 tsubo of the pig house. There was a 78.2% reduction in disease.

Example 4 When 2 L of the diluted solution of Example 2 was sprayed into a pig house having a pneumonia lesion in a pig with respect to 100 tsubo of the pig house, it was 77.2% as compared with the control group which was sprayed or atomized.
The disease was alleviated, the weight gained and the economic effect was exhibited.

Example 5 Pig Actinobacillus pullo
Pneumoniae (Actinobacillus ple)
When a 10-fold dilution of glutaral was inhaled in a pig hut with a risk of 2 ul per 100 tsubo in a pig hut where there was a possibility of the occurrence of three severely ill pigs, two mildly ill pigs became zero. The suppression of PP was completely recognized, and the growth promotion and feed requirement were also improved.

Example 6 In a pig house where there is a risk of respiratory disease in pigs, 5 L of a 50-fold diluted glutaral solution was sprayed on 100 tsubo of a pig house, and compared with the control group which was sprayed or atomized, it was 77.6. % Of pigs gained weight.

Example 7 In a pig house with AR lesions in the turbinates of pigs, when a 100-fold dilution of glutaral was inhaled in a volume of 10 L per 100 tsubo of the pig house, the number of affected lesion pigs was reduced to 4 out of 9 pigs, and the therapeutic effect was reduced. Demonstrated.

[0042]

As described above in detail, according to the present invention, glutaral is diluted to 10 to 100 times the amount thereof,
0.5 to 10 L of fumes kills the causative organisms of respiratory diseases in pigs, resulting in a reduction in mortality and reduction of lung and nasal lesions. It can provide economic effects such as improvement and shortening of the shipping date, and can also have excellent effects of preventing respiratory diseases in pigs.

 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Takenori Saiga 757-1, Futako, Takatsu-ku, Kawasaki, Kanagawa Prefecture Inside (72) Inventor Yoichiro Tsubaki 77-1, Futako, Takatsu-ku, Kawasaki, Kanagawa Prefecture Within Sangyo Co., Ltd. (72) Inventor Junichi Takada Inside Yashima Sangyo Co., Ltd., 757-1 Futako, Takatsu-ku, Kawasaki City, Kanagawa Prefecture

Claims (5)

(57) [Claims] 1. A disinfectant glutaral is diluted with water to which a buffer has been added, and the resulting diluted liquid is smoked with a particle size of 10 μm or less using a fogger or a high-pressure finer, and sucked by a pig. Infiltration of fume particles into the respiratory bronchi of the lower respiratory tract and even into the alveoli, causing the mycop, a respiratory disease-causing bacterium that is resident in pigs
Razuma Hio Nyumonie (Mycoplasma)
hyopneumoniae), Pasteurella maltosi
Da (Pasteurella multitocida),
Actinobacillus pleuropneumoniae (Actino
bacillus pleuropneumonia
e), Haemophilus para-Swiss
us parasuis), Bordetella Bronxep
Chica (Bordetella bronchisept)
A method of treating respiratory disease in pigs, comprising killing ica) . 2. A preparation containing 25% of glutaral is prepared by adding 10 to 10 to 10 parts of water containing approximately the same amount of buffer as the glutaral preparation.
The method for treating respiratory disease in pigs according to claim 1, wherein a dilute solution diluted to 0-fold is used. 3. The diluent prepared per 100 tsubo of pork house is diluted with 0.1 g
The method for treating swine respiratory disease according to claim 1 or 2, wherein fumes of about 5 L to 10 L are produced. 4. A method for preventing respiratory disease in pigs, which comprises periodically performing the method according to claim 1, 2 or 3 to prevent respiratory disease in pigs. 5. A respiratory tract-causing bacterium that is resident in pigs by fogging the particles with a particle diameter of 10 μm or less and inhaling them into the respiratory bronchi of the lower respiratory tract of the pigs and further into the alveoli.
Mycoplasma Hio Nyumonie (Mycopla)
sma hyopneumoniae), Pasteurella
Malto fern (Pasteurellamultocid)
a), Actinobacillus pleuropneumoniae (Act
inobacillus pleuropneumonon
iae), Haemophilus para swiss (Haemph)
ilus parasuis), Bordetella bronchi
Septica (Bordetella bronchise)
A therapeutic agent for killing ptica), comprising diluting a formulation containing 25% glutaral with water containing a buffer equivalent to the glutaral formulation by a factor of 10 to 100 times. Remedy for respiratory disease.