JPH08145796A - Three dimensional spectrum display device - Google Patents

Abstract

PURPOSE: To eliminate complexity of setting or resetting of a vertical axis scale before and after measurement of three dimensional spectrum by storing a maximum value, minimum value or peak information of a spectrum signal during measurement, and correcting the setting of the vertical scale so as to satisfy these information and preliminarily determined restriction conditions. CONSTITUTION: In a three dimensional spectrum, scattered light such as scattering of excitation light in the slant direction, semi-secondary light of the scattered light, and secondary light of the scattered light is observed. At the time of starting the measurement, an appropriate lower limit value and upper limit value of the vertical axis are determined and display of a graph on real time is started. For example, a minimum value and maximum value of the spectrum signal are temporarily determined, each one of spectrum data obtained is compared with these, and at the time point when smaller minimum value or larger maximum value is found, these values are replaced with the new values. These are adopted as the lower limit value and the upper limit value or a new spectrum vertical axis, calculated results are placed with contour line interval, spectrum display up to this time is erased, and re-evaluation is conducted with the new scale. This operation is repeated.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a three-dimensional spectrum display device.

[0002]

2. Description of the Related Art An example of three-dimensional spectrum measurement by a spectrofluorometer is Nissan Sangyo Co., Ltd., Hitachi, Ltd.
It is described in TECHNICAL DATA, FL No. 37. Here, the setting method of the vertical axis scale and the contour line interval in the contour line display is not described, and the measurer determines the suitable vertical axis scale and the contour line interval by the scale changing function after the measurement.

[0003]

In the prior art, in the three-dimensional measurement in the spectrofluorometer, the operator inputs and sets the vertical axis scale (including contour line intervals if contour lines are displayed) before measurement. Was being measured. In this method, in the case of continuously measuring a plurality of samples with greatly different emission intensities, the vertical scale must be changed for each measurement in order to confirm the details of the sample by the three-dimensional spectrum. No, it is complicated. In addition, since a spectrofluorometer equipped with a function to detect the maximum value of the light emission from the sample prior to the measurement is also commercially available, it is possible to use this function for each measurement and set the vertical scale. However, this function has the property of exerting its effect when the sample is set in the sample chamber, and is not suitable for a sample that easily changes with time or a sample that is easily transformed by the irradiation of excitation light.

In three-dimensional chromatography, it is very difficult to set a suitable scale prior to measurement.

The present invention eliminates the complexity of setting or resetting the vertical scale before and after the three-dimensional spectrum measurement, and always displays the three-dimensional spectrum in real time with a suitable vertical scale.

[0006]

[Means for Solving the Problems] The above problem is to store maximum and minimum values or peak information of a spectrum signal during measurement, and set a vertical axis scale so as to satisfy these information and preset constraint conditions. Achieved by reworking.

[0007]

The above problem is that the maximum value, the minimum value, or the peak position of the spectrum signal is detected and stored during the measurement, and the vertical axis scale is updated and displayed. Therefore, it is not necessary to set the vertical axis scale before and after the measurement. . In addition, by providing a constraint condition when detecting the maximum value and the minimum value, it becomes possible to display the target portion in the three-dimensional spectrum with a more suitable vertical scale. For example, in the case of a three-dimensional spectrum obtained by a spectrofluorometer, if the maximum value or the minimum value detected from this generation position is excluded by limiting the generation position of the excitation light scattering, its higher-order light, Raman scattering, or its higher-order light. Only the fluorescent component can be displayed on a suitable vertical scale, and if it is a three-dimensional chromatogram, if the retention time of the target component is known in advance, this time is the constraint condition and the maximum detected around this time. By using only the values and the minimum values, the three-dimensional spectrum can be displayed on a scale suitable for the target component.

[0008]

Embodiments of the present invention will be described below with reference to the drawings.

FIG. 1 shows a typical three-dimensional spectrum from a spectrofluorometer. Scattered light such as scattered excitation light, half-order light of scattered light, and secondary light of scattered light is observed in an oblique direction. These are often strongly observed by diffuse reflection on the surface, especially when measuring a solid sample.

In actual measurement, the EX wavelength (excitation wavelength) is set to the minimum value (200 nm) within the measurement range, the EM wavelength (fluorescence wavelength) is scanned, and after the scanning is completed, the excitation wavelength is changed to
The operation of setting a wavelength larger by a fixed wavelength (here, set to 5 nm) and scanning the fluorescence wavelength is repeated until the measurement of the maximum wavelength value (500 nm) within the excitation wavelength measurement range is completed.

Conventionally, a vertical axis scale and contour line intervals are set prior to measurement, and a three-dimensional spectrum is displayed according to these values.

In the present invention, at the start of measurement, the graph is started to be displayed in real time with appropriate lower and upper limit values for the vertical axis (for example, the lower and upper limits are set as 0-100). Here, for example, the minimum value of the spectrum signal is tentatively set to 10000, and the maximum value is set to 0, and the acquired spectrum data is compared with each other,
When a smaller minimum value or a larger maximum value is found, these values are replaced with new values. This is set as the lower limit value and upper limit value of the new spectrum vertical axis of the vertical axis, and the calculation result of (upper limit value-lower limit value) / 10 is set as the contour line interval,
The three-dimensional spectrum that has been displayed so far is erased from the display unit and redrawn with a new scale. Furthermore, the comparison of the new minimum and maximum values and the data point is restarted from the data point next to the detected spectrum data point, and when the minimum and maximum values are updated, the new vertical scale and contour interval are restarted. The operation of setting and redrawing the three-dimensional graph is repeated. However, if the spectrum data value gradually increases, the 3D graph will be erased and redisplayed for each data point, and it will take a lot of time to display it. When a value is detected, the minimum value is multiplied by, for example, 0.75, and the value is replaced as the minimum value. For the new maximum value, the maximum value is multiplied by 1.25, and the maximum value is changed. You may proceed with the process of deleting and re-displaying the graph by adding processing such as replacing.
Instead of comparing the minimum value data points, a condition such as always setting 0 may be added.

By the way, when the above-mentioned minimum value and maximum value retrieval processing is executed in the state of FIG. 1 and a three-dimensional spectrum is displayed, when the vertical axis scale is set for scattered light of excitation light or its higher order light. Is the most. Here, the fluorescence peak of the sample should be observed along the fluorescence wavelength axis on the longer wavelength side than the wavelength of the excitation light (observed in the lower right region of the excitation light in Fig. 1), and the scattering of the excitation light And its higher-order light are observed at the position of the fluorescence wavelength of (excitation wavelength × n) (where n is 1/2, 1, 2, 3 ...
* Represents). Based on this, the following constraint conditions are set in advance when detecting the maximum value.

1) Ignore the maximum value detected at a fluorescence wavelength smaller than the excitation wavelength.

2) Ignore the maximum value detected in the region of (excitation wavelength × n) − (bandwidth of spectroscope) to (excitation wavelength × n) − (bandwidth of spectroscope). However, n = 1 /
Set to 2, 1, 2.

By adding these two constraint conditions, it is possible to exclude the part caused by scattered light and its higher-order light from the factors that determine the vertical axis scale, and determine the vertical axis scale by the intensity of only the light emission from the sample. It will be possible.

Next, another embodiment will be described. FIG. 2 is a three-dimensional spectrum of a spectrofluorometer, but shows an example in which the influence of excitation light scattering and Raman scattering cannot be ignored.

The excitation wavelength is set to 200 nm, the fluorescence wavelength is scanned from 200 nm to 500 nm, the peak in the fluorescence wavelength scanning range is detected by the processing device, and the fluorescence wavelength value and the spectrum data value are stored. Next, the excitation wavelength is set to 205 nm, the fluorescence wavelength is scanned, and the peak within the scanning range is detected. The peak positions of scattering of excitation light and Raman scattering (similarly when each higher-order light is present) move when the excitation wavelength is changed, and the peak position of emission due to fluorescence does not move. Set constraints.

1) Of the peaks detected by the previous wavelength scanning on the fluorescence side and the peaks detected by the current wavelength scanning, the larger one of the peaks whose positions have not changed is adopted as the maximum value.

2) If no peak satisfying 1) is detected, the maximum value is not updated.

3) When the peak satisfying 1) is not detected at the end of the measurement, the constraint condition for detecting the maximum value shown in the example of FIG. 1 is applied to the spectrum data to draw the spectrum.

By providing these constraint conditions when the maximum value is detected, it becomes possible to display the light emission from the sample on a suitable vertical scale.

In addition, if the peak position of the sample to be observed is known in advance, a constraint condition may be set such that the maximum value detected near this peak position is adopted as the maximum value.

In addition, if the retention time of the component to be observed is known in advance in the three-dimensional chromatogram, setting the constraint condition that the maximum value detected in the vicinity of this time is adopted as the peak is The components can be observed in real time on a suitable vertical scale.

[0025]

According to the present invention, a three-dimensional spectrum can be observed in real time on a suitable vertical scale.

[Brief description of drawings]

FIG. 1 is an explanatory diagram of a fluorescence three-dimensional spectrum that is greatly affected by scattered light and its higher-order light.

FIG. 2 is an explanatory diagram of fluorescence three-dimensional spectrum and peak detection that are greatly affected by Raman scattering.

Claims (3)

[Claims] 1. A detector for detecting a spectrum signal, a storage device for storing the spectrum signal, an image display device for reading the storage information of the storage device and displaying it on a screen, an acquisition of the spectrum signal and a spectrum A data processing device for displaying and processing data, wherein the spectral signal is determined as a function of two factors P1 and P2, and the image display device comprises the factors P1 and P2 of the storage device. What is displayed as a contour map, a bird's-eye view, a gradation display according to the spectrum signal intensity or a combination thereof in a coordinate system, the maximum value or the minimum value of the spectrum signal or the P1 axis or P2 axis during the measurement of the spectrum signal The value and position of the peak along
Stored in the storage device, the vertical axis scale of the spectrum display is automatically set to the difference between the maximum value and the minimum value of the spectrum signal or a constant multiple thereof, and a three-dimensional spectrum is displayed. When a spectrum signal is detected, the maximum or minimum value is updated, the three-dimensional spectrum displayed by the old scale is deleted, and a new maximum or minimum value is updated based on the updated new maximum or minimum value. A three-dimensional spectrum display device, characterized in that the three-dimensional spectrum is re-displayed with a vertical axis scale, and the three-dimensional spectrum is displayed with a suitable vertical axis scale at the end of measurement. 2. A detector for detecting a spectrum signal, a storage device for storing the spectrum signal, an image display device for reading the storage information of the storage device and displaying it on a screen, an acquisition of the spectrum signal and a spectrum A data processing device for displaying and processing data, wherein the spectral signal is determined as a function of two factors P1 and P2, and the image display device comprises the factors P1 and P2 of the storage device. What is displayed as a contour map, a bird's-eye view, a gradation display according to the spectrum signal intensity or a combination thereof in a coordinate system, the maximum value or the minimum value of the spectrum signal or the P1 axis or P2 axis during the measurement of the spectrum signal The value and position of the peak along
When stored in the storage device, the vertical axis scale of the spectrum display is automatically set to the difference between the maximum value and the minimum value of the spectrum signal or a constant multiple thereof, and the spectrum signal which is larger or smaller by the end of the measurement is detected. The maximum value or minimum value is updated, a new vertical scale is set again based on the updated new maximum value or minimum value, and the three-dimensional spectrum is re-set with a suitable vertical scale at the end of measurement. A three-dimensional spectrum display device characterized by displaying. 3. The factor P according to claim 1 or 2, when the maximum value or the minimum value of the spectral signal is detected.
1, P2, the preset constraint that only the maximum or minimum value of the spectral signal detected under the specific P1, P2 condition is stored as a new maximum or minimum value of the maximum or minimum value. A three-dimensional spectrum display device which is applied as a constraint condition at the time of detection to automatically set a vertical axis scale in a three-dimensional display and which displays a three-dimensional spectrum with a suitable vertical scale at the end of measurement.