JPH08143891A - Perfume composition - Google Patents

Abstract

PURPOSE: To obtain an essence being extracted from lavender of a specific mating species, showing excellent enhancing action on immunological function through fragrance, useful as an immunopotentiator, a cosmetic and an aroma for raising immunological function. CONSTITUTION: This essence is an essence of lavender of a mating species made by using a true lavender (Lavandula angustiforia) as a mother and a spike lavender (Lavandula latiforia) as a father. The essence is obtained by castrating a not flowered lavender two or three days before an expected day of mating, bagging the lavender, picking a stamen of a spike lavender on the expected day of mating, applying pollen to the pistil of the true lavender, germinating a seed obtained from a pollinated flower, raising the seed to obtain a plant, selecting an excellently raised individual having an improved color to give a lavender of mating species, drying and finely cutting the lavender and subjecting the finely cut lavender to steam distillation or extracting it with an organic solvent.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an essence of a hybrid lavender produced by using genuine lavender (Lavandula angustifolia) as a mother and spike lavender (Lavandula latifolia) as a father, and an immunopotentiator comprising the same. The present invention relates to a fragrance composition containing: an external preparation for skin, a bath preparation, a cleansing agent, and an fragrance containing the same.

[0002]

2. Description of the Related Art Immunity is an important function of the human body to protect itself from infectious diseases, etc. When immunity is weakened, immunity is seen from a fatal day as seen in acquired immunodeficiency disease (AIDS). It is known that infection can be life threatening.

Although immunostimulants such as krestin and picibanil have been developed, they are effective for treating cancer but not effective for infectious diseases, and are expensive.
Since the route of administration was also an injection, it could not be handled easily. Therefore, there is no means for enhancing immunity against infectious diseases.

[0004] Conventionally, lavender has been widely cultivated as a fragrance plant for taking perfume oil and the like. It has also been used as tea as a herb for tea and other purposes. However, it has not been known that perfume oils or herbal products produced from these lavenders have the effect of enhancing immunity.

[0005] Attempts have not yet been made to enhance immunity by utilizing scents such as fragrances.

[0006]

Therefore, the present invention provides a fragrance, a fragrance composition, a cosmetic composition containing the fragrance, which enhances immunity,
Another object of the present invention is to provide a fragrance containing the same.

[0007]

[Means for Solving the Problems] In view of the above situation, the inventors of the present invention have conducted earnest studies, and as a result, produced genuine lavender (Lavandula angustifolia) as a mother and spike lavender (Lavandula latifolia) as a father. It was discovered that the hybrid lavender (hereinafter referred to as the improved lavender) that had a scent that had the effect of enhancing the immune function, and applied for a patent. (Japanese Patent Application No. 5-178255) As a result of further detailed research,
It was confirmed that the fragrance composition obtained from this improved lavender also has an action of enhancing the immune function through the scent.
It was found that a cosmetic or fragrance that enhances immune function can be provided by forming this fragrance composition into a cosmetic or a fragrance, and completed the invention.

The improved lavender used in the present invention is described in detail in the above-mentioned Japanese Patent Application No. 5-178.
As described in 255, it has a special immunopotentiating effect as compared with other lavenders, and its production method is to emanate unflowered true lavender about 2-3 days before the scheduled mating date, The bag is hung up, the stamens of spiked lavender are picked with tweezers on the scheduled mating day, pollen is applied to the female core of genuine lavender, seeds are obtained from the pollinated flowers, this is germinated, and this is grown to obtain a plant. It was Improved lavender can be obtained by selecting individuals having good growth and excellent flower color among these plants. At this time, the flower color should be selected based on the following. That is, the surface color of the upper flower color is 10.0 PB5.0 in the color table according to JISZ8721.
/9.0 is out.

The improved lavender thus obtained contains a fragrance component having an action of enhancing immunity to flowers and plants as a whole, and enhances immunity by extracting the fragrance component from flowers or whole plants by a conventional method. It is possible to obtain a perfume ingredient having an action. Specific examples of the method for extracting the fragrance component include a steam distillation method, a pressing method, a solvent extraction method, and a distillation method. A substance having an immunopotentiating effect can be extracted by any method. Considering the extraction efficiency, the steam distillation method and the solvent extraction method are preferable. Of these, in the solvent extraction method,
As the solvent to be used, hydrocarbons such as hexane and petroleum ether, ethers such as diethyl ether, acetone,
One or more selected from ketones such as methyl ethyl ketone, alcohols such as methanol and ethanol, halogenated hydrocarbons such as chloroform and methylene chloride, and the like can be exemplified. The solvent extraction may be carried out by immersing it in the solvent at room temperature for several days, or by immersing it in the vicinity of the boiling point of the solvent for several hours while stirring. Then, the insoluble matter is removed by filtration or the like, and then concentrated to obtain a fragrance component.

Before such extraction work, it is possible to pretreat the improved lavender flowers or whole grass so that the extraction work can be performed easily. Examples of the pretreatment include drying and finely cutting the plant.

By the way, the essence referred to in the present invention is
The product obtained by the extraction work, the product obtained by the extraction work further purified by column chromatography or the like,
It means a dried product of the whole plant or a part of it, or a sliced product of the dried product.

The essence thus obtained can be mixed with other fragrances to give a fragrance composition for perfuming. At this time, the preferable blending amount of the essence of the present invention is 0.
1 to 90% by weight is suitable. This is because if the amount is less than 0.1% by weight, the immunopotentiating effect cannot be expected, and if the amount exceeds 90% by weight, the effect reaches the ceiling. The essence of the present invention can be blended into various dosage forms such as an external preparation for skin, a bath agent, a cleansing agent, and a fragrance according to a conventional method either as a fragrance composition containing the essence or as an essence alone.

The essence of the present invention, together with optional components such as hydrocarbons, oils and fats, fatty acids, alcohols, humectants, surfactants, preservatives, antioxidants, powders, thickeners and water, It can be formulated into a skin external preparation such as cream, emulsion or lotion by a conventional method. At this time, the blending amount of essence with respect to the total amount of the external preparation for skin is preferably 0.1 to 10% by weight.
This is because if it is less than 0.1% by weight, an immune enhancing effect cannot be expected, and if it exceeds 10% by weight, the scent is too strong and it is not suitable as a skin external preparation.

The essence of the present invention is an inorganic salt,
It can be formulated into various bath agents together with optional components such as surfactants, humectants, preservatives, or antioxidants. At this time, the blending amount of the essence with respect to the total amount of the bath agent is preferably 0.1 to 10% by weight. This is because if it is less than 0.1% by weight, an immune enhancing effect cannot be expected, and if it exceeds 10% by weight, the scent is too strong and it is not suitable as a bath agent.

Further, the essence of the present invention can be formulated into a detergent together with optional components such as a surfactant such as soap, a coloring agent, a moisturizing agent, a preservative and a thickener. At this time, the blending amount of essence with respect to the total amount of the cleaning agent is preferably 0.1 to 10% by weight. This is because if it is less than 0.1% by weight, the immune enhancing effect cannot be expected, and if it exceeds 10% by weight, the scent is too strong and the scent remains after washing, which is not preferable.

Furthermore, the essence of the present invention is formulated into an aromatic agent such as eau de toilette, eau de parfum, perfume, room fragrance, car fragrance, etc. together with an excipient, an antioxidant, a surfactant, a carrier and the like according to a conventional method. it can. At this time, the blending amount of the essence with respect to the total amount of the aromatic is 0.1.
~ 90 wt% is preferred. This is because if the amount is less than 0.1% by weight, the immunopotentiating effect cannot be expected, and if the amount exceeds 90% by weight, the effect reaches the ceiling.

[0017]

The present invention will be described in more detail below with reference to examples, but it goes without saying that the present invention is not limited to these examples.

Example 1 Production Example 1 kg of the whole lavender plant with flowers was packed in a 20-liter round bottom flask, steam was blown into the flask to effect steam distillation, and the water phase portion of the liquid fraction obtained was cooled. After discarding, 4 g of liquid essence was obtained.

Example 2 Production Example 1 kg of whole lavender whole plant with flowers was packed in a 20 l round-bottomed flask, 5 l of ethanol was added thereto, and the mixture was heated under reflux for 2 hours and cooled to remove insoluble matter by filtration. After concentration under reduced pressure, 17 g of liquid essence was obtained.

Example 3 Formulation Example The following ingredients were weighed out and mixed by stirring at room temperature to obtain a fragrance composition. Essence of Example 2 50 Linalyl acetate 20 Bergamot essence 25 Synthetic musk ketone 5

Example 4 Formulation Example The following components were weighed and solubilized by stirring at room temperature to obtain a lotion. Polyethylene glycol (400) 5 Propylene glycol 2 POE (60) Hydrogenated castor oil 1 Ethanol 10 Methylparaben 0.2 Perfume composition of Example 3 0.2 Purified water 81.6

Example 5 Formulation Example The following components A, B and C were weighed out, respectively, heated to 80 ° C. and dissolved, and B was gradually added to A while stirring.
Further C was added and emulsified. Cool this while stirring,
An emulsion was obtained. (A) Cetanol 1 Squalane 4 Stearic acid 1 Essence of Example 10 10 Butylparaben 0.1 Stearyl stearate 3 Stearyl monoglyceride 0.5 POE (25) Stearic acid 2.5 (B) Purified water 40 Caustic potassium 0.3 ( C) Purified water 27.2 Propylene glycol 10 Methylparaben 0.2 Carbopol 0.2

Example 5 Formulation Example The following components A, B and C were weighed and heated to 80 ° C. to dissolve them, and B was gradually added to A while stirring,
Further C was added, emulsified, and cooled with stirring to obtain a cream. (A) Cetyl stearate 3 Vaseline 5 Olive oil 5 Liquid paraffin 32 BHT 0.1 Butylparaben 0.1 Sorbitan monostearate 2.5 POE (20) Sorbitan monostearate 2.5 Essence 5 of Example 1 (B) ) Purified water 25 10% aqueous caustic potash solution 1.5 (C) Purified water 11.1 Propylene glycol 7 Methylparaben 0.2

Example 6 Formulation Example The following components were heated and stirred at 80 ° C. to dissolve, and the mixture was stirred and cooled to obtain a shampoo. N-myristyl-β-alanine 7 Palm oil fatty acid diethanolamide 3 POE (20) lauryl ether sulfate triethanolamine 10 propylene glycol 10 methylparaben 0.3 Essence of Example 1 0.2 Purified water 69.5

Example 7 Formulation Example The following components were heated and stirred at 80 ° C., dissolved, stirred and cooled to obtain a body shampoo. N-myristic acid-β-alanine 10 Lauric acid 12 Triethanolamine 10 Propylene glycol 7 Essence of Example 2 6 Purified water 55

Example 8 Formulation Example The following components were weighed in a kneader, heated and kneaded at 80 ° C., rolled and molded to obtain soap. Lauric acid 20 Stearic acid 10 Oleic acid 5 Behenic acid 1 34% aqueous sodium hydroxide solution 18 Ethanol 18 White sugar 14 Cetanol 1 Glycerin 5 Fragrance composition of Example 3 5 Purified water 3

Example 9 Formulation Example The following components were weighed in a kneader and kneaded to obtain a bath agent. Sodium Sulfate 91 Essence of Example 2 8 POE (40) Behenyl Ether 1

Example 10 Formulation Example The following components were weighed out, heated and stirred, dissolved and then cooled to obtain a bath agent. Palm oil fatty acid diethanolamide 1 Butylparaben 0.1 Methylparaben 0.2 Ethanol 15 Propylene glycol 10 Fragrance composition of Example 3 0.2 Purified water 73.5

Example 11 Formulation Example The following ingredients were weighed out, mixed by heating with stirring, filled in a mold and cooled to obtain a room fragrance. Fragrance composition of Example 3 10 Carnauba wax 40 Microcrystalline wax 30 Castor oil 15 Sorbitan monostearate 5

Example 12 Formulation Example The following ingredients were weighed out, and mixed and filtered with stirring to obtain an eau de toilette. Essence of Example 2 20 Triethyl citrate 10 Benzyl alcohol 5 Ethanol 50 Purified water 15

Example 13 Formulation Example The following components were weighed, stirred, mixed and filtered, and filled in a glass bottle equipped with a cotton core to obtain a room fragrance. Essence 90 of Example 2 Diethyl phthalate 5 Ethanol 5

Example 14 Measurement of Immunity-Enhancing Effect With respect to the lotion of Example 4, changes in the concentration of secretory immunoglobulin A in saliva before and after its use were measured to determine the immunity-enhancing effect of the cosmetic composition of the present invention. I confirmed. That is,
A lotion of 10 people in each group was used, and the essence of Example 2 was replaced with a normal lavender oil for the lotion of Example 4 (Example) and the fragrance composition in the lotion of Example 4 (Comparative Example). , Saliva was collected before and after use of the lotion in three groups of the perfume composition of Example 3 in which the perfume composition of Example 3 was replaced with water (control example), and secretory immunoglobulin A in saliva was collected. Was measured by the sandwich antibody method of Nishida et al. ("The History of Medicine" 129 (11),
767-770, 1984).

Here, the method for measuring secretory immunoglobulin A will be described in detail. A crude goat anti-human α-chain antibody (IgG fraction, CAPELL Co.
(Manufactured by K.K.) was physically adsorbed and insolubilized. On the other hand, 0.2%
0.05M phosphate buffer (pH 7.5, buffer A below containing BSA, 1% NaCl and 0.05% Tween 20)
Say ) Standard secretory immunoglobulin A (s
-IgA, CAPPEL Co. Manufactured) solution 100 μl,
Alternatively, 100 μl of a sample obtained by diluting saliva 400 times with buffer A
Add l. Here, above polystyrene ball 1
Then, add 400 parts in buffer A as the second antibody.
Peroxidase-labeled rabbit anti-SC antibody (DAK
300 μl (manufactured by OPATTS) was added. 37 ° C, 4
After the time incubation, the polystyrene balls were washed 3 times with 1 ml of buffer A. Then 0.2
0.1M citric acid-0.1M phosphate buffer (p containing 5% o-phenylenediamine and 0.015% hydrogen peroxide (p
H4.7) Transfer the polystyrene balls to a test tube containing 500 μl and incubate at 37 ° C. for 30 minutes,
2 ml of 1N sulfuric acid was added to the test tube to stop the enzymatic reaction, and the absorbance was measured at 492 nm. The concentration of secretory immunoglobulin A was calculated from the ratio of the absorbances of the standard solution and the test solution. Table 1 shows the rate of increase in the concentration of secretory immunoglobulin A after using the lotion as compared with before use. Than this,
It can be seen that the lotion of the present invention has an excellent immune enhancing effect.

[0034]

[Table 1]

Example 15 Immunopotentiating Effect A panel of 10 persons per group was allowed to stay in a closed room with room fragrance for 30 minutes, and the concentration of secretory immunoglobulin A in saliva before and after the stay Was measured. The room fragrance used was the room fragrance of Example 13 (Example), or the room fragrance of Example 13 in which the essence of Example 2 was replaced with normal lavender oil (Comparative Example). The method for measuring the concentration of secretory immunoglobulin A was in accordance with Example 14. Table 2 shows the results. From this, it is understood that the room fragrance of the present invention has an excellent immune enhancing effect.

[0036]

[Table 2]

[0037]

The fragrance, the fragrance composition, the cosmetic, the bath agent, the detergent and the fragrance of the present invention are very useful because they have an immunopotentiating effect.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification number Internal reference number FI Technical indication location A61K 7/46 A 301 7/50 35/78 ABD Q 8217-4C ADA (72) Inventor Chiho Suga Child 27-1 Takashimadai, Kanagawa-ku, Yokohama-shi, Kanagawa Pola Chemical Industry Co., Ltd. Yokohama Research Laboratory

Claims (7)

[Claims] 1. An essence of a hybrid lavender produced by using genuine lavender (Lavandula angustifolia) as a mother and spike lavender (Lavandula latifolia) as a father. 2. An immunopotentiator comprising the essence according to claim 1. 3. A fragrance composition comprising the essence according to claim 1. 4. The fragrance composition according to claim 3, wherein the content of the essence according to claim 1 is 0.1 to 90% by weight. 5. The essence according to claim 1 is 0.1 to 1.
An external preparation for skin containing 0% by weight. 6. The essence according to claim 1 is 0.1 to 1.
A bath agent containing 0% by weight. 6. The essence according to claim 1 is 0.1 to 1.
Detergent containing 0% by weight. 7. The essence according to claim 1 is 0.1 to 9
Fragrance containing 0% by weight.