JPH0667817B2 - Cosmetic composition containing asparagus saponin - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to a cosmetic composition containing an asparagus extract, and particularly imparts at least one of antifungal properties, surface-active ability and hair-growth effect. Alternatively, it relates to a cosmetic composition formulated in a significantly improving amount.

[Prior Art] It has been well known that saponins are contained in plants. In general, saponin is a glycoside containing a complex alicyclic compound as an aglycone, and is a general term for a group of plant components whose aqueous solution exhibits foaming properties, fish toxicity, hemolytic properties and the like. Further, animal saponins such as sea cucumber and starfish saponins are also known.

Subsequent studies revealed the properties of saponin itself, and its classification was broadly divided into steroid saponins and triterpenoid saponins.

A solvent extraction method is known as a method for recovering the above-mentioned saponin component from these plants and animals.

On the other hand, cosmetics are compositions in which various active ingredients including surfactants and preservatives are blended in appropriate proportions.

Surfactants are incorporated for the purpose of imparting properties such as emulsifying property, foaming property and wettability to cosmetics. As these surfactants, chemically synthesized products such as sodium lauryl sulfate and polyoxyethylene sorbitan fatty acid ester are mainly used.

The preservative is added for the purpose of preventing contamination of various bacteria from fingers when using. As these antiseptics, benzoic acid and its derivatives, chemically synthesized products such as propionic acid and sorbic acid are mainly used.

In addition, there is a group of cosmetics that are classified as a hair nourishing agent in terms of efficacy and effect, and various agents that bring out a hair nourishing effect are blended. Chemically synthesized products such as capronium chloride, estradiol, and resorcin are mainly used as these drugs.

[Problems to be Solved by the Invention] However, when the above-mentioned chemical synthetic product is mixed as a surfactant, an antiseptic, or a hair nourishing agent, skin problems such as itch, rash and rough skin may occur. Therefore, in recent years, extracts such as crude drugs have been attracting attention in place of the above-mentioned chemically synthesized products. However, although crude drugs are superior in safety to conventional chemically synthesized products, no one has yet been found that exerts sufficient effects as surfactants, preservatives and hair nourishing agents. Is.

[Means for Solving the Problems] The inventors of the present invention have conducted extensive studies as a result of solving the problems, and as a result, an extract of asparagus which is an edible plant or a saponin which is a part of the components thereof has been identified. The present invention has been completed by discovering that it has an excellent antifungal effect as compared with conventional chemically synthesized products, and also has excellent surface-active ability and hair-growth effect.

That is, the present invention is a cosmetic composition characterized by incorporating an asparagus extract as an active ingredient, and more specifically, the asparagus extract is asparagus saponin and / or asparagus saponin-containing extract. The present invention relates to a cosmetic composition.

[Working] Asparagus extract used in the present invention means asparagus (Asparagus officinalis L.) stems, rhizomes, leaves,
It is a mixture of various active ingredients obtained by extraction from flowers and the like.

The extraction method is not particularly limited, for example, as an extraction solvent,
Water, primary alcohols such as methyl alcohol and ethyl alcohol, polyhydric alcohols such as propylene glycol and 1,3-butylene glycol, lower alkyl esters such as acetic acid ethyl ester, hydrocarbons such as benzene and hexane,
Examples thereof include ethyl ether, and these can be used alone or in combination of two or more. Among them, the preferable extraction solvent includes an aqueous solution of an organic solvent miscible with water, particularly an aqueous solution of ethyl alcohol, butyl alcohol, acetone and the like. It may be an extract obtained by extracting with an extraction solvent at room temperature or while heating, and filtering, or a concentrate thereof. Furthermore, if necessary,
These extracts can be purified and used. Specific examples of such concentrated or purified products include Japanese Patent Application No. 63-279349 “Saponin recovery method from asparagus waste” and Japanese Patent Application No. 1-67510 “Purified saponin from asparagus” by the present inventors. In the method disclosed in "Method of recovering a.", Through an extraction step using asparagus or its rhizome or waste portion usually used for food as a raw material,
The powdery saponin itself, which can be produced at low cost, or the extract obtained in each extraction step can be used according to its use.

As a form of use thereof, a powdered saponin component can be used directly or by dissolving or dispersing the powder in a solvent such as water or alcohol, and in the case of an extract, water or alcohol can be similarly used. It can be used after being dissolved or dispersed in a solvent such as.

These asparagus extracts can be used alone or in combination of two or more obtained in each extraction step, and further other preservatives, surfactants and hair-growing agents of the present invention can be used. It may be used in combination within a range that does not impair the effect.

The amount of the asparagus extract compounded in the cosmetic composition of the present invention can be appropriately determined depending on the intended effect. For example, from various test results, when formulated for the purpose of suppressing the reproduction of fungi, usually 0.001 ~ 0.5
The range of wt.% is appropriate, and more preferably 0.01-0.1w
t.%. When compounded for the purpose of imparting or improving a surfactant action such as emulsifying property and foaming property, usually 0.5 to 20.
A range of 0 wt.% Is suitable, and more preferably 1.0 to 10.0 w
t.%. Moreover, when blending for the purpose of a hair-growth effect, the range of 0.0001 to 10.0 wt.% Is usually appropriate, and more preferably 0.001 to 5.0 wt.%. However, the above range is given as a reference for determining an appropriate blending amount, and is not a numerical limitation in the sense that no effect is obtained outside the range. Further, the blending amount is the content of the dry solid content as a solute, and when using the extract, it is sufficient to adjust the amount of the extract so that the content of the dry solid is within the above range. Thus, the concentration of the extract itself does not require any special limitation.

Examples of cosmetic compositions comprising the asparagus extract of the present invention include emulsions, lotions, basic cosmetics such as creams, hair tonics, hair liquids, hair creams,
Examples include hair cosmetics such as shampoo and conditioner, and cosmetics (quasi-drugs) of various dosage forms such as soap.

Further, in the composition of the present invention, in addition to the above-mentioned essential components, purified water, lower alcohols, polyhydric alcohols, oils and fats, which are usually used in cosmetic compositions, in a range not impairing the effects of the present invention,
Beauty ingredient, UV absorber, water-soluble polymer, pigment, powder,
A fragrance or the like can be appropriately selected and used.

Hereinafter, the present invention will be described in detail with reference to production examples and examples. However, the present invention is not limited to these examples.

[Production Example 1] 23,650 g of raw white asparagus root as a raw material
Measured (as fresh weight), then 60% alcohol
It adjusted so that it might become%, and ethanol was added.

These were ground using a food mixer (National MX-M3) to obtain a mixture.

Then, a mixture consisting of these raw materials and a solvent was put in a poly bucket and allowed to stand at room temperature for one day to extract, and then the extract was collected by suction filtration into a suction filtration bottle.

To the extraction residue, 25% of 60% ethanol was added again, and after repeating the above-mentioned operation, about 66 of the extract was concentrated by a rotary evaporator and the solvent was distilled off to obtain a brown concentrated extract of 1,00.
0 g was obtained (first step).

Next, a mixed solvent of n-butanol and water (1: 1) was added until the concentrated extract was completely dissolved, and the mixture was shaken well and then centrifuged at about 10,000 rpm for 30 minutes with a centrifuge. , Saponin components, etc. were extracted and separated into the n-butanol layer (second
Process).

Then, the n-butanol layer was concentrated by a rotary evaporator and the solvent was distilled off to obtain about 110 g of an extract (third step).

Then, water and benzene were added to the extract in an equivalent amount and suspended to obtain a milky white solution. This solution is centrifuged at 10,000 rpm for 30 minutes using a centrifuge, and the separated benzene layer is tilted by centrifuging the centrifuge tube to discard the benzene in the upper layer.
Alternatively, a pipette tube was used to remove only the upper layer by suction and remove the lipid component in the extract, and the same amount of fresh benzene was added to the remaining aqueous layer to carry out the same operation.
The degreasing step may be performed by adding only benzene, but it has been confirmed that efficient degreasing can be performed by using another solvent such as chloroform or ether (fourth step).

Then, water was concentrated from the obtained aqueous layer portion by a rotary evaporator and dried to dryness, and then about 1 of a mixed solvent of n-butanol and water (1: 1) was added to dissolve the extract, and the mixture was placed in a separating funnel. After that, the saponin component was extracted into the butanol layer by standing still for 24 hours.

Then, the butanol layer was concentrated and dried by a rotary evaporator to obtain about 18 g of extract (brown extract) (fifth step).

Next, 300 ml of methanol was added to the brown extract to dissolve it, and 75 ml of each was slowly dropped into a separately prepared ethyl ether (2) using an injection needle to form a white precipitate, which was allowed to stand for a while. After that, most of the ether was removed by the gradient method, and the precipitate was collected by suction filtration,
The precipitate was washed several times with fresh ether to wash away the dissolved ether of contaminants.

The almost white precipitate obtained by such an operation was dried in a vacuum desiccator and then ground in a mortar to obtain about 11 g of asparagus saponin powder (sixth step).

[Example 1] Using the asparagus saponin powder obtained in the sixth step of Production Example 1, a cream was produced by a conventional method according to the formulation shown in Table 1 below, and the antifungal effect was confirmed.

The antifungal properties of the obtained cream were tested under the following conditions, and it was found that the cream of the present invention had sufficient desired effect.

That is, M containing Candida albicans (IFO1061) was used using the cream of the present invention and a control cream having a composition obtained by removing asparagus saponin from the composition shown in Table 1, respectively.
A filter paper disc (φ6 mm) thinly coated with each cream on Y agar medium was placed so that the surface of the cream was in contact with the agar, and cultured overnight at a temperature of 37 ° C., the cream of the present invention was obtained. On the coated filter paper, formation of a transparent inhibition circle in which bacteria did not grow was observed, whereas on the paper coated with the control cream, no inhibition circle was observed.

[Example 2] Using the asparagus saponin powder obtained in the sixth step of Production Example 1 and the asparagus extract obtained by dissolving the butanol fraction in the fifth step of Production Example 1 in methanol, the surface activity of these components was determined. The ability was evaluated based on a usual test method in terms of foaming property, emulsifying property, wettability, solubilizing property, and dispersibility.
The results are shown in Table 2.

As can be seen from the results in Table 2, the asparagus saponin powder or the asparagus extract has almost the same foamability, wettability, and dispersibility as the standard contrast agent Tween80 (polyoxyethylene sorbitan trioleate). It was confirmed to have an effect.

Next, the emulsion stability and the like of creams A and B containing the above two components were evaluated by leaving them at 40 ° C. for 2 months. The cream formulation is as shown in Table 3.

The creams A and B of the present invention were not inferior in both emulsion stability and feeling of use when compared with those prepared using a conventional chemically synthesized surfactant.

[Example 3] The formulations shown in Table 4 using the asparagus saponin powder obtained in the sixth step of Production Example 1 and the asparagus extract of the butanol layer portion obtained in the third step of Production Example 1 as samples. The sample was prepared and the hair-feeding effect was evaluated by the test method shown below.

Hair nourishing effect test method: Male C3H / HeNCrj mice (8 weeks old) were made into one group of 10 mice,
The test was conducted. That is, about 2 x 4 cm of the back of the mouse was shaved, and the samples (1) to (3) were applied once a day from the next day every day, and the part where the skin color began to change from pink to gray (hair regeneration) (The part where the beginning of the) started, the evaluation points (0 to 5) for the area of the part that entered the regeneration period and the evaluation points (0 to 5) of the results of observation with the naked eye regarding the state of the regeneration period were evaluated according to the following criteria, respectively. The total score (0 to
Using 10) as the evaluation score for one mouse, the average score of the two evaluators was calculated. The results are shown in Table 5.

As is clear from Table 5, the hair nourishing agent of the present invention had a remarkable hair regeneration promoting effect as compared with the hair nourishing agent of the sample (3) as the control.

[Example 4] Cream formulation example (formulation A): A cream was produced according to the above-mentioned formulation A by the following procedure.

I. (1) to (10) are heated to 75 ° C. and mixed and dissolved.

II. (11)-(14) are heated to 75 ° C and mixed and dissolved.

Add II to III.I, mix, add (15) and cool.

The thus-prepared cream of the present invention was excellent in antifungal effect and emulsion stability, and had a good feeling in use.

[Example 5] Prescription example of hair nourishing agent (prescription B): Based on the above-mentioned prescription B, the hair nourishment was manufactured in the following procedure.

I. (1) to (4) and (6) and (8) are mixed and dissolved.

II. (5) and (7) are mixed and dissolved.

Add II to III.I.

The hair nourishing agent of the present invention thus produced was excellent in the hair regeneration promoting effect.

[Effects of the Invention] As described above, the cosmetic composition of the present invention is important in terms of antifungal properties, surface-active ability, hair-growth effect, and the like, which are conventionally imparted to cosmetics by blending various agents obtained by chemical synthesis. These properties are satisfied by the combination of extracted components obtained from natural and edible plant asparagus, so it is safe for human body, has no health hazard, and itches even after long-term use. There is no risk of skin problems such as rashes and rough skin. Since the blended asparagus extract is particularly excellent in antifungal properties, cosmetics can be used safely without being contaminated with various bacteria for a long period of time. In addition, since the hair nourishing effect is also remarkable, it is possible to manufacture hair cosmetics containing this as a main component, and in this case, the antifungal effect and the surface-active effect are automatically exerted. And a cosmetic composition that is safer for the human body.

Claims (5)

[Claims] 1. A hair nourishing agent containing asparagus saponin or an asparagus saponin-containing extract as a hair growth promoter. 2. The hair nourishing agent according to claim 1, wherein the content of the asparagus saponin or the asparagus saponin-containing extract is 0.0001 to 10.0 wt%. 3. A cosmetic composition characterized by reducing or eliminating the addition amount of a preservative composed of a chemically synthesized product by adding and using asparagus saponin or an asparagus saponin-containing extract as an antifungal agent. object. 4. The cosmetic composition according to claim 3, wherein the content of the asparagus saponin or the asparagus saponin-containing extract is 0.001 to 0.5 wt%. 5. A cream comprising 0.001 to 0.5 wt% of asparagus saponin or an asparagus saponin-containing extract as a preservative.