JPH08143406A - Plant growth accelerator - Google Patents

Abstract

PURPOSE: To provide a plant growth accelerator which manifests the excellent action to give the effects of rooting promotion, spindly growth prevention, good seedling breeding, increased rooting rate in cuttage, a shortened period needed for breeding, increased yield or the like. CONSTITUTION: This plant growth promotor comprises (A) glycyrrhizin, (B) at least one vitamin selected from among β-carotene, thiamine, riboflavin, nicotinic acid amide, pyridoxine, ascorbic acid, tocopherol and quercetin as active ingredients. The weight ratio of these components A/B is 1/0.001-1/1000, particularly 1/0.1-1/10. The component A is an active ingredient of licorice, and a crushed product, an extract or a crude product of licorice may be directly used without isolation and purification. This growth accelerator is usually applied in the form of aqueous solution of 0.01-10,000mg/l, particularly of 0.5-500mg/l concentration.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a plant growth promoter containing glycyrrhizin and specific vitamins as active ingredients.

[0002]

2. Description of the Related Art In recent years, the dangers to the human body of chemically synthesized pesticides used in golf courses, residual pesticides of agricultural products, etc. have become a problem, and various research institutions are promoting the development of highly safe pesticides. There is. As one example, attempts have been made to use Chinese herbal medicines for fertilizers, agricultural chemicals and the like. For example, crude drug fermented liquid (Alum Green: trade name of Alum Co., Ltd.) is registered as a pesticide for promoting the growth of grass roots and enhancing disease resistance. In addition, JP-A-2-268
In Japanese Patent Publication No. 86, it is proposed to use a fermented product obtained by adding a mineral substance to an extraction residue of a medicinal plant and using it as a fertilizer. In Japanese Patent Laid-Open Publication No. 61-15806, Japanese ginseng, watermelon, loofah, armchair, Plant-extracted saponin extracted from cucumber, melon or licorice,
A plant growth promoter containing a fermentation product as an essential component has been reported.

Regarding the effect of adding various vitamins to plants, for example, nicotinic acid amide, choline chloride, vitamin K ₃ salt and the like have been reported as plant growth regulators. Furthermore, in Japanese Patent Laid-Open No. 4-59711,
It has been reported that the mixed use of a herbal medicine and a certain kind of vitamin exhibits a high plant growth promoting effect which is not seen when these herbal ingredients and vitamins are used separately.

[0004]

The present invention has the effect of further increasing the vitality of Chinese herbal medicines against plants and promoting growth,
That is, it provides a plant growth promoter having improved performance, which further stabilizes the effects of rooting promotion, prevention of overgrowth, growth of healthy seedlings, improvement of survival rate in cuttings, reduction of period required for growth, improvement of yield, etc. To do.

[0005]

Means for Solving the Problems As a result of intensive studies, the present inventors have found that glycyrrhizin in licorice, which is one of Chinese herbal medicines, and at least one of vitamins selected from a specific vitamin group as active ingredients. By combining the above, for plants, compared to the case of using various herbal medicines or vitamins known to date,
The inventors have found that they have further excellent effects, and have completed the present invention.

That is, the present invention comprises glycyrrhizin and
β-carotene (vitamin A precursor), thiamine (vitamin B ₁ ), riboflavin (vitamin B ₂ ), nicotinamide (vitamin B ₃ ), pyridoxine (vitamin B ₆ ),
It is intended to provide a plant growth promoter characterized by containing at least one vitamin selected from ascorbic acid (vitamin C), tocopherol (vitamin E) and quercetin (vitamin P) as an active ingredient.

Glycyrrhizin is a compound known as an active ingredient of licorice, and in the present invention, it can be used as a licorice crushed product, a licorice extract or a crude product without separation and purification. When licorice or licorice extract is fermented, glycyrrhizin is decomposed, and the intended effect of the present invention is lost.
Fermentation of licorice or licorice extract is not desirable. Further, in the present invention, it can be purified and used as glycyrrhizin or a salt thereof.
Examples thereof include metal salts such as sodium, potassium, lithium, calcium, magnesium, manganese, iron, zinc and the like, ammonium salts and the like, and the kind thereof is not limited as long as it is not a particularly harmful salt. In addition, these glycyrrhizins and salts thereof may be used alone or in admixture of two or more.

The vitamins used are all commercially available and any of them can be used. In general, its salt forms, for example, metal salts such as sodium, potassium, lithium, calcium, magnesium, manganese, iron, zinc and ammonium salts, hydrochlorides, nitrates,
There are sulfates, acetates, citrates, malates, etc., but the types thereof are not limited unless they are particularly harmful. Further, these metal salts and the like can be used alone or in combination of two or more kinds.

The plant growth promoter of the present invention comprises glycyrrhizin and at least one vitamin selected from β-carotene, thiamine, riboflavin, nicotinamide, pyridoxine, ascorbic acid, tocopherol and quercetin by a method known per se. It is possible to mix them into a dosage form such as a powder, granules, emulsifiers, and liquids, although not particularly limited thereto. Glycyrrhizin and vitamins are mixed in a weight ratio of 1: 0.001 to 1: 1000, preferably 1: 0.01 to 1: 100, and more preferably 1: 0.1 to 1:10 in terms of the effect. .

If desired, the plant growth promoter of the present invention can be further formulated with additives such as sugars, amino acids, organic acids, alcohols and minerals, and in order to enhance the effect thereof. Other pesticides, such as fungicides, insecticides, plant hormones, etc., fertilizers, natural mineral substances, such as barley stones, Io stones, etc., may be prescribed together.

The plant growth promoter of the present invention is not particularly limited, but is applied to foliar spraying treatment, soil irrigation treatment, seed or cutting treatment, or hydroponic culture, addition to a culture solution for tissue culture. It can be applied to plants by treatment and the like, and is usually 0.01 to 100 as an aqueous solution, although it varies depending on the type of plant.
00 mg / liter, preferably 0.1-1000 mg
/ Liter, more preferably 0.5-500 mg / liter. The plant to be applied is not particularly limited, for example, rice, wheat, grains such as corn, radish, carrot, tomato, komatsuna, vegetables such as Chinese cabbage, mandarin orange, peach,
Fruit trees such as grapes, flowers such as chrysanthemums, tulips, and pansies, beans such as peas, broad beans, soybeans, potatoes such as potatoes and sweet potatoes, lawns such as sweet potatoes, hornworts, leeks such as onions, onions, and lacquer trees. , Grass such as alfalfa, clover, Satsuki, Japanese smelt,
Examples include trees such as cedar and cypress. Further, the plant growth promoter of the present invention can be used in combination with other pesticides, fertilizers and the like.

[0012]

EXAMPLES Next, the present invention will be described in more detail with reference to experiments and examples, but the present invention is not limited thereto. Unless otherwise specified, the chemicals used in the following experiments and examples are commercial grade reagents. Experiment 1 Effect of fermented licorice extract on initial growth of Komatsuna 5 g of licorice extract (glycyrrhizin content: about 3%
(Hereinafter, referred to as GL)) to 100 ml of water, and 0.5 g of each of two types of commercially available microbial agents (Bimfood Co., Ltd. Shimamoto Microorganisms Co., Ltd., EM-1 Japan Food Co., Ltd.), respectively. The culture was carried out at 30 ° C. for 5 days with stirring and static culture, respectively. The solution was diluted 100-fold and used in a germination test using komatsuna and the GL content was quantified by HPLC. For germination test, 1 on the filter paper in a petri dish.
0 ml was added. 20 pieces of komatsuna were inoculated on the filter paper, and after culturing at 25 ° C. for 5 days, stem length and root length were measured. The results are shown in Table 1. The results in Table 1 are ratios to control (water only) cultures. GL was quantified by HPLC using a reverse layer ODS column after pretreatment with a Seppak column. As a result, GL decreased with the progress of fermentation, and the growth promoting effect on the roots of Komatsuna decreased. Thus, when the fermented liquor of the licorice extract was used, the growth promoting effect was rather decreased.

[0013]

[Table 1] Culture conditions Culture days GL content, stem length (%) root length (%) mg / liter 0 248 127 180 stirred culture 2 124 138 140 beim hood 5 24 132 116 0 335 109 171 static culture 2 178 127 147 EM-1 5 Not detected 127 125

Example 1 Glycyrrhizin (GL) and β-carotene (PrV.A), thiamine (VB ₁ ), riboflavin (VB ₂ ), nicotinamide (VB ₃ ), pyridoxine (VB ₆ ), ascorbic acid ( VC), tocopherol (VE) and quercetin (V.
P) is an aqueous solution having a concentration shown in Table 2 below (pH 6.0).
Then, 10 ml of each was added on the filter paper in the petri dish. 20 seeds of Komatsuna are sown on this filter paper, and 25
The root length was measured after culturing at 5 ° C. in the light for 5 days. Table 2 shows the results based on the root length without addition (100%). As is clear from Table 2, the plant vitalizing agent of the present invention showed a remarkable growth promoting effect, which was superior to the case where each compound was used alone.

[0015]

[Table 2] (Concentration: mg / liter) No. GL PrV.A VB ₁ VB ₂ VB ₃ VB ₆ VC VE * VP Index% 1 100 2 100 157 3 10 104 4 100 10 168 5 100 10 10 202 6 10 158 7 100 10 162 8 100 10 10 259 9 10 107 10 100 10 168 11 100 10 10 233 12 10 118 13 100 10 190 14 100 10 10 217 15 10 110 16 100 10 168 17 100 10 10 184 18 10 130 19 100 10 188 20 10 99 21 100 10 165 22 100 10 10 176 23 10 104 24 100 10 188 25 100 10 10 205 * A tenfold amount of surfactant (Tween 20) was used for emulsification.

Example 2 Crude glycyrrhizin having a purity of 60% and choline chloride (C
h), phytic acid (Ph), ascorbic acid (V.
C), Murashige-Skoog inorganic salt medium (hereinafter referred to as MS), and a comparison of the effects on the growth of Komatsuna using a commercial plant growth promoter containing a herbal herb fermented liquid (trade name of Alum Green Co., Ltd. Alum) investigated. Table 3
As shown in Fig. 10, 10% of each concentration of drug was placed on the filter paper in the petri dish.
ml was added. Put 20 seeds of Komatsuna on this filter paper.
The root length was measured after sowing the seeds, culturing for 5 days at 25 ° C. in the light. The concentration of the commercially available growth promoter was used as the optimum concentration (all diluted 1000 times). Table 3 shows the results based on the root length in the case of no addition (100%). As is clear from Table 3, GL and V. The test solution in which C was used in combination showed a remarkable growth promoting effect as compared with the other solutions, and it was possible to further enhance the effect of the fertilizer by adding it to another growth promoting agent or the like.

[0017]

[Table 3] Plant growth promoter MS ** GL * VC * Ph * Ch * index% 1/2 100 1/2 100 162 1/2 100 10 197 1/2 100 20 174 1/2 100 10 10 171 Chinese medicine Crude drug fermented liquid 138 Kampo crude drug fermented liquid 100 10 181 Commercial product A 119 Commercial product A 100 10 168 Commercial product B 122 Commercial product B 100 10 159 *: Concentration display mg / l **: MS medium is 1/2 the normal concentration The amount was doubled.

Example 3 100 mg / liter and 10 mg of crude glycyrrhizin having a purity of 60% and ascorbic acid (VC), respectively.
/ L and the pH is adjusted to 6 with calcium hydroxide.
Each aqueous solution having 0 was prepared. By the same method as in Example 2, 20 seeds of rice, leeks, cabbage, and tomato were sown, and after culturing, the stem length and the root length were measured. The culture conditions are as follows. The results are shown in Table 4. The numerical values in the table show the elongation ratio of the added plot to the stem length and root length of the non-added plot. As is clear from Table 4, GL and V.I. When C is used in combination, a more excellent growth promoting effect is recognized as compared with the case where each is used alone, and it is particularly remarkable in roots. Culture conditions Rice: 25 ° C, dark place, 10 days Leek: 25 ° C, light place, 7 days Cabbage: 25 ° C, dark place, 7 days Tomato: 25 ° C, light place, 10 days

[0019]

[Table 4] (Concentration: mg / liter) Plant name GL VC Stem length (%) Root length (%) 100 100 Rice 10 121 115 100 97 148 100 10 109 172 100 100 Negi 10 89 91 100 110 151 100 10 121 168 100 100 Cabbage 10 81 129 100 100 127 100 10 98 141 100 100 Tomato 10 109 102 100 94 139 100 10 91 144

[0020]

Effect of the Invention Glycyrrhizin and β-carotene, thiamine, riboflavin, nicotinic acid amide, pyridoxine,
By using at least one selected from ascorbic acid, tocopherol and quercetin, it shows an excellent growth promoting effect on plants, and as a result, rooting promotion, prevention of overgrowth, healthy seedling growth, improved survival rate in cuttings, etc. The effect of shortening the required period and improving the yield can be obtained.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Office reference number FI technical display area A01N 43:78) (A01N 43/16 43:90) (A01N 43/16 43:40) (A01N 43/16 43:08)

Claims (11)

[Claims] 1. A plant growth promoter comprising glycyrrhizin and at least one vitamin selected from β-carotene, thiamine, riboflavin, nicotinamide, pyridoxine, ascorbic acid, tocopherol and quercetin as active ingredients. Agent. 2. The plant growth promoter according to claim 1, wherein the weight ratio of glycyrrhizin: vitamin is 1: 0.001 to 1: 1000. 3. The plant growth promoter according to claim 1, which contains glycyrrhizin and β-carotene as active ingredients. 4. The plant growth promoter according to claim 1, which contains glycyrrhizin and thiamine as active ingredients. 5. The plant growth promoter according to claim 1, which contains glycyrrhizin and riboflavin as active ingredients. 6. The plant growth and plant promoting agent according to claim 1, which contains glycyrrhizin and nicotinic acid amide as active ingredients. 7. The plant growth promoter according to claim 1, which contains glycyrrhizin and pyridoxine as active ingredients. 8. The plant growth promoter according to claim 1, which contains glycyrrhizin and ascorbic acid as active ingredients. 9. The plant growth promoter according to claim 1, which contains glycyrrhizin and tocopherol as active ingredients. 10. The plant growth promoter according to claim 1, which contains glycyrrhizin and quercetin as active ingredients. 11. The plant growth promoter according to claim 1, which is applied to plants as an aqueous solution having a concentration of 0.01 to 10000 mg / liter.