JPH078762B2 - Sterilizer for livestock - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a bactericide for livestock sheds. More specifically, the present invention relates to a bactericide that exhibits excellent effects against bacteria, mold and the like in livestock sheds.

[Conventional technology and its problems]

Livestock farms that raise livestock such as cows, pigs, and chickens are in an environment where excrements of these animals and residual dirt of food are present, and microorganisms are prone to breeding, and pathogenic bacteria, etc. to livestock and humans may occur. The livelihood is extremely high, so it is necessary to clean and sterilize the livestock shed. However, when breeding livestock for meat and eggs, a bactericide with low toxicity to animals and humans and strong antibacterial activity is required.

Although there is no bactericide that can sufficiently meet these requirements, a cationic surfactant having a certain level of antibacterial activity and safety and bactericidal activity is used. Examples of such compounds include alkyldimethylbenzylammonium salt, substituted benzalkonium salt, dialkyldimethylammonium salt and the like.

However, the above cationic surfactants are not sufficiently satisfied with a wide range of sterilization and mold, and when a large amount of excrement such as "dung" adheres to livestock / chicken, it is strongly adsorbed by "dung" and sterilized. There is a drawback that the property significantly decreases.

[Means for solving problems]

Therefore, as a result of advancing the development of compounds having an antibacterial activity and an excellent antibacterial spectrum to solve such problems, the present inventors did not reduce the antibacterial activity even in the presence of excrement, and moreover, including spore-forming bacteria. The present invention has been completed by discovering a bactericide having excellent efficacy against a wide range of microorganisms.

That is, the invention has the general formula (I)(In the formula, R₁And R₂Are each Or an alkyl group having 1 to 4 carbon atoms, at least one of which isAnd R_FiveIs an alkyl or alkenyl group having 4 to 22 carbon atoms
And R₆Is an alkyl or alkenyl group having 2 to 20 carbon atoms
And R₃And R_FourIs an alkyl group having 1 to 4 carbon atoms,
X Is a counter anion. ) A quaternary ammonium salt represented by
The present invention provides a bactericide for livestock housing.

The compound represented by formula (I) according to the present invention is produced by a known method. For example, it is produced by quaternizing a tertiary amine represented by the general formula (II) with a quaternizing agent such as methyl chloride, methyl bromide or dimethylsulfate.

{In formula (II), R ₁ , R ₂ and R ₃ are the same as in formula (I). } The tertiary amine represented by the formula (II) is also produced by a known method. For example, it is produced by a method of directly aminating the alcohol represented by the general formula (III) with methylamine or dimethylamine.

{In formula (III), R ₅ and R ₆ are the same as in formula (I), respectively. Of the compounds represented by the general formula (I) according to the present invention, the compounds represented by the following formula (IV) or (V) are preferable.

(In the formula, n + m = 6 to 18, X is Cl, Br or CH ₃ SO ₄
Is. ) (In the formula, n + m = 6 to 18, X is Cl, Br or CH ₃ SO ₄
Is. The quaternary ammonium salt represented by the general formula (I) used in the present invention is used as an aqueous solution or a water dispersant, and the concentration thereof is usually 50 to 10 although it varies depending on the object.
It is 00 ppm.

In addition, when using the quaternary amonium salt represented by the general formula (I) of the present invention, salts, surfactants, water-soluble thickeners and the like may be used in combination in the preparation, if necessary. I do not care.

Examples of salts include succinic acid, malonic acid, citric acid, gluconic acid, carboxylic acid metal salts such as glutaric acid, tripolyphosphoric acid, hexametaphosphoric acid, phosphoric acid compound metal salts such as phosphoric acid, Na ₂ SO ₄ , MgSO ₄ , etc. And the like. These can be used alone or in combination.

As the surfactant, nonionic surfactants and anionic surfactants are used, and examples of the nonionic surfactants and / or anionic surfactants include polyoxyethylene (hereinafter referred to as POE). ) Alkyl (6 to 2 carbon atoms
2) Non-ion such as ether, POE alkyl (C4-18) phenol ether, polyoxypropylene polyoxyethylene (block or random) alkyl ether, POE phenylphenol ether, POE styrenated phenol ether, POE tribenzylphenol ether Surfactant, lignin sulfonate, alkylbenzene sulfonate, alkyl sulfonate, POE alkyl sulfonate, POE alkyl phenyl ether sulfonate, POE alkyl phenyl ether phosphate ester salt, POE phenyl phenol ether sulfonate , POE phenylphenol ether phosphate salt, naphthalene sulfonate, naphthalene sulfonic acid formalin condensate, POE tribenzylphenol ether sulfonate, POE tribenzyl phenylpheno Anionic surfactants such as ether phosphoric acid ester salts. These can be used alone or in combination. Its content in the fungicide is 0 to 20% by weight, preferably 1 to 10% by weight.

As the water-soluble thickener, natural, semi-synthetic and synthetic water-soluble thickeners can be used, natural mucilage, zansan gum derived from microorganisms, Zanflow, plant-derived pectin,
Gum arabic, guar gum, etc. are semi-synthetic mucilages such as methylated products of cellulose or starch derivatives, carboxyalkylated products, hydroxyalkylated products (including methylcellulose, carboxymethylcellulose, hydroxymethylcellulose, etc.), sorbitol, etc. Acrylate, polymaleate,
Specific examples include polyvinylpyrrolidone and pentaerythritol E0 adduct. The water-soluble thickener is incorporated in the bactericide in an amount of about 0 to 3.0% by weight, preferably about 0.05 to 0.5% by weight.

[Action / effect]

Although the bactericide of the present invention is not necessarily clear about the mechanism of exerting a remarkable bactericidal effect which has not been obtained by the quaternary ammonium salt so far, for sterilization in livestock houses such as cattle, pigs and chickens, etc. , As the alkyl group of the quaternary ammonium salt, It is considered that the introduction of such a branching group causes irreversible denaturation of the cell membrane and kills the bacteria. In addition, it is thought that adsorption is unlikely to occur to the "fun" of livestock housing due to its molecular bulkiness, and reversible desorption occurs even if it is adsorbed.

〔Example〕

Next, the disinfectant of the present invention will be specifically described with reference to examples using various fields, but the disinfectant of the present invention is not limited to these examples.

The structures of the products of the present invention (Nos. 1 to 6) and comparative products (Nos. 1 to 3) used in the examples are shown below.

Invention product No. Invention product No. Comparative product No. Example 1 E. Coli and Klebsiella cultivated in various concentrations of the product of the present invention and the comparative product and then cultured in a liquid medium in advance
Pneumonia (Klebsiella pne-umoniae) was contacted for 5 minutes, then the cells were collected, planted in a new medium, allowed to stand for a certain period of time, and the growth state of these cells was evaluated according to the following criteria.

The results are shown in Table 1.

<Evaluation> + No growth (no colony formation) ± Slight growth (up to 5 colonies) + Growth (5 or more colonies) Example 2 An accurately weighed sample was dissolved in water to prepare each concentration, and 1 m
l was poured into a 9 cm dish. 9m of heated agar medium
It was dispensed, mixed uniformly and solidified. Each plate was inoculated with Aspergillus niger, Cladosporium herbarium, and Botoritis cinerea test strains, and after culturing under certain conditions, the minimum concentration that completely inhibits growth (Minimum inhibitory conce-ntration MIC μg / m
l) was sought.

The results are shown in Table 2.

Example 3 A solution having a concentration of 10,50,100 ppm of the product of the present invention and the comparative product was prepared,
The dried chicken "dung" was added to 0.8% of the mixture, the mixture was shaken and stirred for 10 minutes, and then the chicken "dung" was removed by a centrifuge. After that, Escherichia coli was contacted for 5 minutes, and after harvesting, it was planted in a new medium and left for a certain period of time, and then evaluated by the same method as in Example 1. The results are shown in Table 3.

Claims (1)

[Claims] 1. A general formula (I)(In the formula, R₁And R₂Are eachOr an alkyl group having 1 to 4 carbon atoms, at least one of which isAnd R_FiveIs an alkyl or alkenyl group having 4 to 22 carbon atoms
And R₆Is an alkyl or alkenyl group having 2 to 20 carbon atoms
And R₃And R_FourIs an alkyl group having 1 to 4 carbon atoms,
X Is a counter anion. ) A quaternary ammonium salt represented by
Sterilizer for livestock.