JPH0755800A - Blood-gathering apparatus - Google Patents

Abstract

PURPOSE: To provide a blood collecting instrument which can be filled with a more correct amount of anticoagulant to improve the solubility of the anticoagulant into a blood sample and which prevents formation of microclots in the blood sample collected. CONSTITUTION: This instrument is manufactured by a method of coating a receiving medium with K3 EDTA, comprising processes for preparing a coating solution composed of K3 EDTA and water, selecting the receiving medium to be coated, atomizing the coating solution to cause the solution to cling to the receiving medium, exposing the receiving medium coated with the coating solution to air for the required time to dry the solution, and allowing dry additives to remain there.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood collecting instrument used for performing hematology tests. In particular, the present invention allows loading of blood collection devices with more accurate amounts of anticoagulant and improves the solubility of the anticoagulant in blood samples as well as in blood samples collected. It relates to preventing the generation of micro clots.

[0002]

2. Description of the Related Art Blood collection tubes must have properties such that a filled anticoagulant acts effectively in a test or analysis for medical use, and the material of the blood collection tube does not interfere with the analysis. . Such tests include, but are not limited to, hematology, blood chemistry, blood typing, toxicology analysis and blood drug concentration measurements.

Examples of the method for filling the blood collecting device with the anticoagulant include a freeze drying method, a vacuum drying method, a liquid filling method, and a residue tube wall coating method. However, these conventional techniques are not preferable in the following points. Freeze-drying is undesirable because the anticoagulant can be rehydrated after drying. When the freeze-drying method and the vacuum drying method are used, the anticoagulant is unevenly distributed in the blood collection tube. Further, these conventional techniques have a problem in that only water is not used as a solvent.
For example, residue tube wall coating requires the use of media / binders such as silicones. Such additives add to the cost of the product and increase the likelihood of interacting with other product components, which may affect the measurement results obtained from blood samples collected.

In all of the above methods, in the case of plastic tubes, a volatile solvent, eg Freon, is required to promote drying at low temperatures so as not to damage the tube.

A further disadvantage of the liquid filling method is that the anticoagulant dilutes the blood sample to be measured. As a result, it may affect the analysis result of the blood sample, and the analysis error due to such dilution may exceed 2%.

[0006]

The present invention seeks to remedy these drawbacks. That is, the present invention enables more accurate, uniform and stable filling of dry anticoagulant,
And it aims at improving the solubility of the dried anticoagulant in a blood sample. Furthermore, the present invention aims to make the above improvements without the use of solvents which may be harmful to the environment or the product.

[0007]

SUMMARY OF THE INVENTION The present invention relates to a blood collection device for hematology tests.

The device is a bottomed tubular container made of plastic or glass with an anticoagulant such as tripotassium ethylenediaminetetraacetate (K ₃ ED) on the inner wall of the container.
TA) is coated by the spray coating method.

The coating is applied in a fine mist by a spray coating method using a medium solvent, preferably water, and the anticoagulant is applied to the inner wall. The tubular container having the anticoagulant applied by spraying to the inner wall is more accurately, stably and uniformly applied with the dry anticoagulant, and the dissolution rate of the anticoagulant in the blood sample is improved. Has the advantage. This effect is particularly remarkable when K ₃ EDTA is used as an anticoagulant. Furthermore, spraying the anticoagulant in a fine mist onto the inner wall of the tube substantially increases the surface area of the anticoagulant in contact with blood and at the same time reduces the generation of microclots in the blood sample. .

According to the present invention, the accuracy of anticoagulant filling is improved by the ability to accurately and easily adjust the solution concentration and the volume of solution dispensed. That is, since water is used as the solvent, a highly accurate metering system can be used to bring the solution to the spray coating system. The concentration of K ₃ EDTA at that time is desirably about 0.00020 ml to about 0.00 per square centimeter of the inner surface of the container.
130 ml, most preferably about 0.0007
It is 5 ml. This method improves the filling of the tubing container with the anticoagulant solution, corrects the incorrect distribution of the anticoagulant on the vessel wall, and facilitates dissolution of the anticoagulant during blood collection. To

The anticoagulant solution is a volumetric device (vol
umetric type device), such as a positive
It is preferable, but not limited to, to dispense by metering with a displacement pump. By appropriately determining the concentration of the solution (the amount of the anticoagulant per unit volume of the solution) and the distribution volume, a desired amount of the anticoagulant can be accurately distributed on the inner wall of the device.

The blood collecting device of the present invention may be a vacuum type blood collecting device or a non-vacuum type blood collecting device.

According to a preferred embodiment of the present invention, the dissolution of anticoagulant in a blood sample is improved by a number of factors.
First, K ₂ EDT, which has been conventionally used as an anticoagulant
A or K ₃ EDTA, which is more soluble than Na ₂ EDTA, is used as an anticoagulant. This results in a large contact area between the anticoagulant and blood and a rapid time for the anticoagulant to dissolve in the sample. Also, the increased contact area between the anticoagulant and blood reduces the generation of micro-clots in plasma samples.

Further, water can be used as a solvent without using a volatile solvent such as Freon. Water has the advantage of being less harmful to the product and the environment.

It will be apparent and will be readily apparent to those skilled in the art that various minor changes can be made without departing from the scope and spirit of the invention.

The invention is illustrated by the following examples.
The following example is merely an example, and the present invention is not limited to this example.

[0017]

EXAMPLE 3,600 mg of K ₃ EDTA was added to distilled water 10
Dissolved in ml. Next, this K ₃ ED per test tube
0.010 ml of the TA aqueous solution was sprayed onto the inner wall of 1,000 test tubes by spray coating.

Next, the test tube is dried with air and K _{3 is applied} to the inner wall.
A test tube coated with EDTA was obtained. The test tube was then sealed and sterilized by gamma irradiation with 2.5 megarads.

The product was rated as functional.

 ─────────────────────────────────────────────────── ─── Continued Front Page (72) Inventor Robert Rosada, Twenty Third Street, Astoria, New York, USA 23-63 (72) Inventor Judith A. Raychenbach, Pumpton Plains, NJ, USA , West Parkway 130

Claims (12)

[Claims] 1. A dry additive having an opening, a bottom, and a sidewall having an inner surface and an outer surface and extending from the opening to the bottom, using water as a medium, and subsequently substantially removing the solvent. A blood collecting device in which K ₃ EDTA is applied to the inner surface by a spray application method that allows the residue to remain. 2. The device according to claim 1, wherein the blood collection device is made of plastic or glass. 3. The device according to claim 1, wherein the blood collecting device is a tubular body. 4. The concentration of K ₃ EDTA is about 240 mg / m 2.
4. The device of any one of claims 1-3, wherein the device is from 1 to about 480 mg / ml. 5. The concentration of K ₃ EDTA is about 360 mg / m 2.
l a and, and the blood collection square about 0.00020ml amount of about 0.00130ml per cm K ₃ EDTA on the inner surface of the instrument is sprayed claim 4 device according. 6. The concentration of K ₃ EDTA is about 360 mg / m 2.
a l, and, K ₃ EDTA in an amount of square centimeter per about 0.00075ml the inner surface of the blood collecting device is sprayed claim 5 device according. 7. A coating solution comprising (a) K ₃ EDTA and water is prepared, (b) a receiving medium to be coated is selected, and (c) the coating solution is made into a fine mist and attached to the receiving medium. And (d) a method of coating the receiving medium, which comprises the steps of: (d) drying the solution by applying air to the coated receiving medium for a required time to leave the dried additive. 8. The method of claim 7, wherein the receiving medium is made of plastic. 9. The receiving medium is made of glass.
The method described. 10. The method of claim 7, wherein the receiving medium is a blood collection device. 11. About 240 mg / ml to about 480 mg
K ₃ EDTA at a concentration of / ml is about 0.00020 ml to about 0.001 per square centimeter of the inner surface of the receiving medium.
The method according to claim 7, wherein the amount of 30 ml is sprayed. 12. K ₃ ED at a concentration of about 360 mg / ml.
TA is approximately 0.0 per square centimeter of the inner surface of the receiving medium.
The method according to claim 11, wherein spraying is performed in an amount of 0075 ml.